JP5224539B2 - 特異的遺伝子の多重検出によるSalmonellaTyphimuriumの迅速同定法 - Google Patents
特異的遺伝子の多重検出によるSalmonellaTyphimuriumの迅速同定法 Download PDFInfo
- Publication number
- JP5224539B2 JP5224539B2 JP2009140192A JP2009140192A JP5224539B2 JP 5224539 B2 JP5224539 B2 JP 5224539B2 JP 2009140192 A JP2009140192 A JP 2009140192A JP 2009140192 A JP2009140192 A JP 2009140192A JP 5224539 B2 JP5224539 B2 JP 5224539B2
- Authority
- JP
- Japan
- Prior art keywords
- seq
- base sequence
- set forth
- sequence set
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 title claims description 7
- 108090000623 proteins and genes Proteins 0.000 title description 65
- 238000001514 detection method Methods 0.000 title description 29
- 108091034117 Oligonucleotide Proteins 0.000 claims description 414
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 215
- 241000607142 Salmonella Species 0.000 claims description 179
- 239000002773 nucleotide Substances 0.000 claims description 85
- 125000003729 nucleotide group Chemical group 0.000 claims description 85
- 238000000034 method Methods 0.000 claims description 82
- 230000003321 amplification Effects 0.000 claims description 28
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 21
- 230000008569 process Effects 0.000 claims description 10
- 108020004414 DNA Proteins 0.000 description 378
- 239000013615 primer Substances 0.000 description 183
- 230000000295 complement effect Effects 0.000 description 79
- 230000004544 DNA amplification Effects 0.000 description 51
- 239000000427 antigen Substances 0.000 description 12
- 108091007433 antigens Proteins 0.000 description 12
- 102000036639 antigens Human genes 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 10
- 239000012071 phase Substances 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 244000144972 livestock Species 0.000 description 8
- 238000007400 DNA extraction Methods 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 101100309767 Arabidopsis thaliana SDR3b gene Proteins 0.000 description 6
- 101100386054 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) CYS3 gene Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 101150035983 str1 gene Proteins 0.000 description 6
- 101100365087 Arabidopsis thaliana SCRA gene Proteins 0.000 description 5
- 101100309766 Arabidopsis thaliana SDR3a gene Proteins 0.000 description 5
- 101100532855 Arabidopsis thaliana SDR5 gene Proteins 0.000 description 5
- 101000604054 Homo sapiens Neuroplastin Proteins 0.000 description 5
- 101000806155 Homo sapiens Short-chain dehydrogenase/reductase 3 Proteins 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 101150105073 SCR1 gene Proteins 0.000 description 5
- 101100134054 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NTG1 gene Proteins 0.000 description 5
- 102100037857 Short-chain dehydrogenase/reductase 3 Human genes 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 101100532854 Arabidopsis thaliana SDR4 gene Proteins 0.000 description 4
- 101000668170 Homo sapiens RNA-binding motif, single-stranded-interacting protein 2 Proteins 0.000 description 4
- 102100039690 RNA-binding motif, single-stranded-interacting protein 2 Human genes 0.000 description 4
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 101100102853 Arabidopsis thaliana VTI11 gene Proteins 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 101100365490 Drosophila melanogaster Jon99Ci gene Proteins 0.000 description 3
- 101100533231 Drosophila melanogaster Jon99Ciii gene Proteins 0.000 description 3
- 101150052146 SER2 gene Proteins 0.000 description 3
- 241001138501 Salmonella enterica Species 0.000 description 3
- 241000392514 Salmonella enterica subsp. enterica serovar Dublin Species 0.000 description 3
- 241000607132 Salmonella enterica subsp. enterica serovar Gallinarum Species 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 101150008449 ser3 gene Proteins 0.000 description 3
- 101150077543 st gene Proteins 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 101100256278 Arabidopsis thaliana SCR gene Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 101150027175 SGR1 gene Proteins 0.000 description 2
- 101150083866 SGR2 gene Proteins 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000011331 genomic analysis Methods 0.000 description 2
- 210000004201 immune sera Anatomy 0.000 description 2
- 229940042743 immune sera Drugs 0.000 description 2
- 238000000126 in silico method Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 101150111766 sc gene Proteins 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 102100037425 17-beta-hydroxysteroid dehydrogenase 14 Human genes 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 101100060192 Anopheles gambiae ser4 gene Proteins 0.000 description 1
- 101100151777 Arabidopsis thaliana SYP22 gene Proteins 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- 101100533228 Drosophila melanogaster Jon99Cii gene Proteins 0.000 description 1
- 102100027626 Ferric-chelate reductase 1 Human genes 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 101150072282 HSD17B14 gene Proteins 0.000 description 1
- 101000862406 Homo sapiens Ferric-chelate reductase 1 Proteins 0.000 description 1
- 101000668165 Homo sapiens RNA-binding motif, single-stranded-interacting protein 1 Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102100039692 RNA-binding motif, single-stranded-interacting protein 1 Human genes 0.000 description 1
- 101150031862 SE gene Proteins 0.000 description 1
- 206010039438 Salmonella Infections Diseases 0.000 description 1
- 101100387184 Salmonella enteritidis PT4 (strain P125109) SEN1436 gene Proteins 0.000 description 1
- 101100487011 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) STM4428 gene Proteins 0.000 description 1
- 101100179880 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) iolE gene Proteins 0.000 description 1
- 101100018978 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) iolG gene Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- -1 dTPT Chemical compound 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000004226 microchip electrophoresis Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 206010039447 salmonellosis Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 101150106567 ser1 gene Proteins 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000007862 touchdown PCR Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009140192A JP5224539B2 (ja) | 2008-07-08 | 2009-06-11 | 特異的遺伝子の多重検出によるSalmonellaTyphimuriumの迅速同定法 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008177942 | 2008-07-08 | ||
JP2008177942 | 2008-07-08 | ||
JP2009140192A JP5224539B2 (ja) | 2008-07-08 | 2009-06-11 | 特異的遺伝子の多重検出によるSalmonellaTyphimuriumの迅速同定法 |
Related Child Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011170210A Division JP5224557B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaCholeraesuisの迅速同定法 |
JP2011170239A Division JP5224558B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaGallinarumの迅速同定法 |
JP2011170228A Division JP5279049B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaEnteritidisの迅速同定法 |
JP2011170233A Division JP5279050B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaDublinの迅速同定法 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2010035552A JP2010035552A (ja) | 2010-02-18 |
JP2010035552A5 JP2010035552A5 (de) | 2011-09-22 |
JP5224539B2 true JP5224539B2 (ja) | 2013-07-03 |
Family
ID=42008731
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009140192A Active JP5224539B2 (ja) | 2008-07-08 | 2009-06-11 | 特異的遺伝子の多重検出によるSalmonellaTyphimuriumの迅速同定法 |
JP2011170233A Active JP5279050B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaDublinの迅速同定法 |
JP2011170228A Active JP5279049B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaEnteritidisの迅速同定法 |
JP2011170210A Active JP5224557B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaCholeraesuisの迅速同定法 |
JP2011170239A Active JP5224558B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaGallinarumの迅速同定法 |
Family Applications After (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011170233A Active JP5279050B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaDublinの迅速同定法 |
JP2011170228A Active JP5279049B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaEnteritidisの迅速同定法 |
JP2011170210A Active JP5224557B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaCholeraesuisの迅速同定法 |
JP2011170239A Active JP5224558B2 (ja) | 2008-07-08 | 2011-08-03 | 特異的遺伝子の多重検出によるSalmonellaGallinarumの迅速同定法 |
Country Status (1)
Country | Link |
---|---|
JP (5) | JP5224539B2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108148892A (zh) * | 2018-01-24 | 2018-06-12 | 深圳市儿童医院 | 沙门氏菌的pcr检测引物、核酸杂交膜条、试剂盒及方法 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5727255B2 (ja) * | 2011-02-21 | 2015-06-03 | 日本ハム株式会社 | 多重pcrによるサルモネラ属菌血清型の迅速簡易判別法およびそのプライマーセット |
JP5279051B2 (ja) * | 2011-08-10 | 2013-09-04 | 独立行政法人農業・食品産業技術総合研究機構 | SalmonellaTyphimuriumを検出するためのオリゴヌクレオチドとそれらを用いた血清型迅速同定法 |
US20160201116A1 (en) * | 2013-08-20 | 2016-07-14 | E. I. Du Pont De Nemours And Company | Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium |
CN106226536B (zh) * | 2016-08-01 | 2017-11-10 | 福建省产品质量检验研究院 | 一种沙门氏菌h抗原的位相变异试验方法 |
CN106244690B (zh) * | 2016-08-03 | 2019-06-18 | 扬州大学 | 一种快速鉴定肠炎沙门菌、鸡白痢/鸡伤寒沙门菌以及都柏林沙门菌的多重pcr检测试剂盒 |
CN111733266A (zh) * | 2020-07-13 | 2020-10-02 | 扬州大学 | 一种快速检测沙门菌并鉴定鸡白痢/鸡伤寒沙门菌的pcr检测试剂盒及其应用 |
CN113388539B (zh) * | 2020-12-30 | 2022-05-20 | 广东省微生物研究所(广东省微生物分析检测中心) | 含有特异性分子靶标的沙门氏菌标准菌株及其检测和应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0479885A (ja) * | 1990-07-24 | 1992-03-13 | Kitasato Inst:The | 新規ビルレンスプラスミド及びこれに関連する塩基配列 |
GB9312508D0 (en) * | 1993-06-17 | 1993-08-04 | Bioteknologisk Inst | Compounds |
-
2009
- 2009-06-11 JP JP2009140192A patent/JP5224539B2/ja active Active
-
2011
- 2011-08-03 JP JP2011170233A patent/JP5279050B2/ja active Active
- 2011-08-03 JP JP2011170228A patent/JP5279049B2/ja active Active
- 2011-08-03 JP JP2011170210A patent/JP5224557B2/ja active Active
- 2011-08-03 JP JP2011170239A patent/JP5224558B2/ja active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108148892A (zh) * | 2018-01-24 | 2018-06-12 | 深圳市儿童医院 | 沙门氏菌的pcr检测引物、核酸杂交膜条、试剂盒及方法 |
CN108148892B (zh) * | 2018-01-24 | 2021-07-27 | 深圳市儿童医院 | 沙门氏菌的pcr检测引物、核酸杂交膜条、试剂盒及方法 |
Also Published As
Publication number | Publication date |
---|---|
JP2011212026A (ja) | 2011-10-27 |
JP5224557B2 (ja) | 2013-07-03 |
JP5224558B2 (ja) | 2013-07-03 |
JP5279050B2 (ja) | 2013-09-04 |
JP2011212027A (ja) | 2011-10-27 |
JP2011254829A (ja) | 2011-12-22 |
JP5279049B2 (ja) | 2013-09-04 |
JP2010035552A (ja) | 2010-02-18 |
JP2011212028A (ja) | 2011-10-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5224558B2 (ja) | 特異的遺伝子の多重検出によるSalmonellaGallinarumの迅速同定法 | |
Olive et al. | Principles and applications of methods for DNA-based typing of microbial organisms | |
Chen et al. | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources | |
Blackall, JK Miflin et al. | Identification and typing of Pasteurella multocida: a review | |
Mohammadi et al. | Comparison of three PCR-based methods for simplicity and cost effectiveness identification of cutaneous Leishmaniasis due to Leishmania tropica | |
Klancnik et al. | PCR in food analysis | |
Bailey et al. | Serotyping and ribotyping of Salmonella using restriction enzyme PvuII | |
Shekhawat et al. | Random amplified polymorphic DNA-based molecular heterogeneity analysis of Salmonella enterica isolates from foods of animal origin | |
Gand et al. | Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java | |
Debruyne et al. | Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli | |
JP2005517396A (ja) | 病原性大腸菌用高分解能タイピングシステム | |
Galluzzi et al. | Current molecular techniques for the detection of microbial pathogens | |
WO2023021978A1 (ja) | 自己免疫疾患を検査する方法 | |
JP5279051B2 (ja) | SalmonellaTyphimuriumを検出するためのオリゴヌクレオチドとそれらを用いた血清型迅速同定法 | |
JP5224541B2 (ja) | SalmonellaHadarを検出するためのオリゴヌクレオチドとそれらを用いた血清型迅速同定法 | |
JP2024509840A (ja) | 蛍光振幅調整を使用する単一プローブによる多重化遺伝子型判定アッセイ | |
Margos et al. | Species identification and phylogenetic analysis of Borrelia burgdorferi sensu lato using molecular biological methods | |
EP2539458B1 (de) | Multiplexverstärkungsreaktionsverfahren zur festlegung von eingekapseltem campylobacter-jejuni-penner | |
Phraephaisarn et al. | Multiple-locus variable-number tandem repeat analysis using multiplex polymerase chain reaction and next-generation sequencing-A novel high-throughput method for subtyping Listeria strains | |
Hashemi et al. | Multiple-locus variable-number tandem-repeat analysis in Salmonella isolates as an effective molecular subtyping method | |
CN114480680B (zh) | 检测沙门菌血清型抗原基因分型的引物组及其应用 | |
JP5224559B2 (ja) | SalmonellaInfantisを検出するためのオリゴヌクレオチドとそれらを用いた血清型迅速同定法 | |
CN113774156B (zh) | 印第安纳沙门菌及其三种血清抗原同时检测实时荧光定量pcr引物、探针、试剂盒和方法 | |
CN111197094B (zh) | 用于副溶血弧菌基因分型的组合物、试剂盒和方法 | |
Boldis et al. | Molecular typing of Coxiella burnetii: a review of available methods with major focus on PCR-based techniques |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110803 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20110803 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20121226 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130207 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130304 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130308 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5224539 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160322 Year of fee payment: 3 |
|
S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |