JP5193216B2 - Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用 - Google Patents

Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用 Download PDF

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JP5193216B2
JP5193216B2 JP2009534568A JP2009534568A JP5193216B2 JP 5193216 B2 JP5193216 B2 JP 5193216B2 JP 2009534568 A JP2009534568 A JP 2009534568A JP 2009534568 A JP2009534568 A JP 2009534568A JP 5193216 B2 JP5193216 B2 JP 5193216B2
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desaturase
seq
gene
sequence
nucleic acid
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JP2010508019A (ja
JP2010508019A5 (cg-RX-API-DMAC7.html
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チーシオン・シュエ
ナレンドラ・エス・ヤダヴ
クィン・クン・チュー
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2009534568A 2006-10-30 2007-07-19 Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用 Expired - Fee Related JP5193216B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85517706P 2006-10-30 2006-10-30
US60/855,177 2006-10-30
PCT/US2007/016490 WO2008054565A1 (en) 2006-10-30 2007-07-19 Δ17 desaturase and its use in making polyunsaturated fatty acids

Publications (3)

Publication Number Publication Date
JP2010508019A JP2010508019A (ja) 2010-03-18
JP2010508019A5 JP2010508019A5 (cg-RX-API-DMAC7.html) 2010-09-02
JP5193216B2 true JP5193216B2 (ja) 2013-05-08

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JP2009534568A Expired - Fee Related JP5193216B2 (ja) 2006-10-30 2007-07-19 Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用

Country Status (8)

Country Link
US (3) US7556949B2 (cg-RX-API-DMAC7.html)
EP (1) EP2087105B1 (cg-RX-API-DMAC7.html)
JP (1) JP5193216B2 (cg-RX-API-DMAC7.html)
CN (1) CN101617040B (cg-RX-API-DMAC7.html)
AU (1) AU2007314481B2 (cg-RX-API-DMAC7.html)
CA (1) CA2663807C (cg-RX-API-DMAC7.html)
DK (1) DK2087105T3 (cg-RX-API-DMAC7.html)
WO (1) WO2008054565A1 (cg-RX-API-DMAC7.html)

Families Citing this family (27)

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US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US9175318B2 (en) 2008-12-18 2015-11-03 E. I. Dupont De Nemours And Company Reducing byproduction of malonates by yeast in a fermentation process
CN102595917A (zh) 2009-06-16 2012-07-18 纳幕尔杜邦公司 来自改善的优化的解脂耶氏酵母菌株的高二十碳五烯酸油
EP2475679B1 (en) 2009-06-16 2016-02-10 E. I. du Pont de Nemours and Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CA2763424C (en) 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
JP5920890B2 (ja) * 2010-01-19 2016-05-18 ディーエスエム アイピー アセッツ ビー．ブイ． エイコサペンタエン酸生成微生物、脂肪酸組成物、ならびにそれらを作る方法およびそれらの使用
CA2795460A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
CA2805882A1 (en) 2010-08-11 2012-02-16 E. I. Dupont De Nemours And Company Improved aquaculture meat products
CA2805891A1 (en) 2010-08-11 2012-02-16 Scott E. Nichols A sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
US8658413B2 (en) 2010-08-26 2014-02-25 E I Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
AU2011293180B2 (en) 2010-08-26 2017-03-02 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
KR101964168B1 (ko) 2010-10-01 2019-04-01 고쿠리쓰다이가쿠호진 규슈다이가쿠 스트라메노파일의 형질 전환 방법
US8735137B2 (en) 2010-12-30 2014-05-27 E I Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA3026436A1 (en) 2011-02-11 2012-08-16 E. I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2952183A1 (en) 2011-07-21 2015-12-09 DSM IP Assets B.V. Fatty acid compositions
EP2935591B1 (en) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
JP2016165226A (ja) * 2013-07-12 2016-09-15 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
WO2015029966A1 (ja) 2013-08-27 2015-03-05 国立大学法人京都大学 ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
JP2017195777A (ja) * 2014-08-29 2017-11-02 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
JP6026709B1 (ja) 2014-12-25 2016-11-16 国立大学法人京都大学 新規ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
CN106010993B (zh) * 2016-06-12 2019-07-05 福建师范大学 生产亚麻酸的圆红冬孢酵母及其制备方法
WO2019209241A1 (en) 2018-04-23 2019-10-31 Dupont Nutrition Biosciences Aps Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid

Family Cites Families (15)

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DE69917668T2 (de) * 1998-10-23 2005-05-19 Sosei Co., Ltd. Substanz gm-95, verfahren zu ihrer herstellung und ihre verwendung
EP1315570A4 (en) * 2000-08-03 2006-12-13 Caliper Life Sciences Inc METHOD AND DEVICES FOR FLUID PROMOTION WITH HIGH THROUGHPUT
US6635451B2 (en) * 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
WO2005049805A2 (en) * 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
PL1720988T3 (pl) 2004-02-27 2012-05-31 Basf Plant Science Gmbh Sposób wytwarzania nienasyconych kwasów tłuszczowych omega-3 w organizmach transgenicznych
EP1723220B1 (de) * 2004-02-27 2013-04-10 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
US7192762B2 (en) * 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7189559B2 (en) 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7198937B2 (en) * 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
DE102005013779A1 (de) * 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
TWI258780B (en) * 2005-04-29 2006-07-21 Asustek Comp Inc Button structure
US7465793B2 (en) * 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
AU2007287585B2 (en) 2006-08-24 2013-08-29 Basf Plant Science Gmbh Isolation and characterization of a novel Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains

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Publication number Publication date
WO2008054565A1 (en) 2008-05-08
CA2663807A1 (en) 2008-05-08
EP2087105A1 (en) 2009-08-12
CN101617040A (zh) 2009-12-30
EP2087105B1 (en) 2015-02-25
JP2010508019A (ja) 2010-03-18
CA2663807C (en) 2018-05-01
CN101617040B (zh) 2012-07-04
US7556949B2 (en) 2009-07-07
US20090280996A1 (en) 2009-11-12
US20130011846A1 (en) 2013-01-10
US8241884B2 (en) 2012-08-14
US20080125326A1 (en) 2008-05-29
DK2087105T3 (da) 2015-05-26
AU2007314481A1 (en) 2008-05-08
AU2007314481B2 (en) 2012-11-29

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