JP5191525B2 - Testosterone 5α-reductase inhibitor and androgen receptor binding inhibitor, anti-androgen hormone agent, hair nourishing cosmetic, and prostate hypertrophy inhibitor - Google Patents

Description

  The present invention relates to a testosterone 5α-reductase inhibitor and an androgen receptor binding inhibitor, an anti-androgen hormone agent, a hair nourishing cosmetic, a prostate hypertrophy inhibitor, and a health supplement. Specifically, a novel testosterone 5α-reductase inhibitor that inhibits the action of testosterone 5α-reductase, which reduces testosterone to active 5α-dihydrotestosterone (active 5α-DHT), and 5α-DHT generated from testosterone Novel androgen receptor binding inhibitor that inhibits binding to receptor, testosterone 5α-reductase inhibitory activity and / or novel antiandrogen agent comprising plant extract having androgen receptor binding inhibitory activity, and testosterone 5α -It relates to a hair nourishing cosmetic, a prostate hypertrophy inhibitor and a health supplement using a reductase inhibitor and / or the androgen receptor binding inhibitor.

  Many steroid hormones are expressed in the form of molecules secreted from production organs and bind to receptors to express their effects. In the case of male hormones collectively called androgens, for example, testosterone enters the cells of target organs and becomes testosterone 5α. -It is reduced to 5α-DHT by reductase and then binds to the receptor, thereby expressing the action as an androgen.

  Androgen is an important hormone, but when it acts excessively, it induces various unfavorable symptoms such as androgenetic baldness, hirsutism, seborrhea, acne, benign prostatic hyperplasia, prostate tumor, premature boyhood, etc. . Therefore, conventionally, in order to improve these various symptoms, a method for suppressing the action of excess androgen, specifically, by inhibiting the action of testosterone 5α-reductase which reduces testosterone to active 5α-DHT. A method for suppressing the generation of active 5α-DHT and a method for inhibiting the binding of 5α-DHT generated from testosterone to the receptor to prevent the expression of androgenic activity were investigated. As a result, cyproterone acetate, The effectiveness of oxendron, chlormadinone acetate, etc. was confirmed.

  However, since these are steroid hormone derivatives, they have the disadvantage of having undesirable side effects such as hormone-like effects.

  On the other hand, testosterone 5α-reductase inhibitory action (for example, see JP-A-60-1466829), action of inhibiting the binding of 5α-DHT and its receptor with respect to some crude drugs and plant extracts from plant bodies ( For example, Japanese cedar cedar, persimmon, hooded umbrellas, and centripetal lotus, which are active ingredient extraction raw materials in the present invention, are known as testosterone 5α-reductase inhibitors. The action of and the action as an androgen receptor binding inhibitor were not known, and the use as an anti-androgen agent was not known.

  An object of the present invention is to find a novel plant extract having testosterone 5α-reductase inhibitory action and androgen receptor binding inhibitory action, to provide a powerful and safe anti-androgen agent, and to provide treatment and prevention suitable for the above symptoms. It is to provide a novel prostatic hypertrophy inhibitor and the like.

  The testosterone 5α-reductase inhibitor according to the present invention is characterized by comprising one or more plant extracts selected from the group consisting of camellia, hooded umbrellas, and centripetal lotus. In addition, the androgen receptor binding inhibitor is characterized by comprising one or more plant extracts selected from the group consisting of camellia, hooded umbrellas and centripetal lotus.

  The anti-androgen hormone agent according to the present invention is one or more plant extracts selected from the group consisting of camellia, hooded umbrellas, and centripetal lotus, and has a testosterone 5α-reductase inhibitory action and / or It is characterized by having an androgen receptor binding inhibitory action.

  Furthermore, the hair nourishing cosmetic, the prostate hypertrophy inhibitor and the health supplement according to the present invention each contain the testosterone 5α-reductase inhibitor and / or the androgen receptor binding inhibitor according to the present invention.

According to the present invention, a novel testosterone 5α-reductase inhibitor and androgen receptor binding inhibitor with few side effects can be provided. With these inhibitors, as an androgen receptor, male and female baldness, hirsutism, seborrhea, acne, benign prostatic hyperplasia, prostate tumor, premature male sexuality, etc., excessive amounts of testosterone or 5α-DHT can be The combination can greatly contribute to the improvement or prevention of each symptom.

  Hereinafter, the present invention will be described in more detail. The testosterone 5α-reductase inhibitor according to the present invention is selected from the group consisting of red bean cedar, lantern, horsal umbrella, and senshinren. It consists of one or two or more selected plant extracts, and contains a testosterone 5α-reductase activity inhibitor as an active ingredient.

  In addition, the androgen receptor binding inhibitor according to the present invention is one or more plant extracts selected from the group consisting of red cedar, bamboo shoots, hooded umbrellas and centripetal lotus, and androgen receptor binding An inhibitory substance is contained as an active ingredient.

  Furthermore, the anti-androgenic hormone agent according to the present invention is one or more plant extracts selected from the group consisting of red bean cedar, birdcage, hooded umbrella and centripetal lotus, and has a testosterone 5α-reductase inhibitory action. And / or has an androgen receptor binding inhibitory action.

  In the present invention, the above-mentioned plant is usually used in a dry state, and its use site is not limited, but from the viewpoint of extraction efficiency, a site belonging to each plant is preferably used as described below. Specifically, red bean cedar (Coutosan) is Taxus chinensis (Pilg.) Rehd., An evergreen tree, and the above-ground part is preferably used mainly. The uranium is an orchid family Canarium pimela Koenig, which is an evergreen tree, and leaves are preferably used in the present invention. Further, the crow umbrella is an evergreen Takagi plant, Heteropanax fragrans (Roxb.) Seem., And the leaves are preferably used in the present invention. Senshinren is a plant, Andrographis paniculata (Burm. F.) Nees, which is a vegetation, and its leaf portion is preferably used. It is to be noted that centrifugal lotus is known for its prevention of white hair, improvement effect (see JP-A-4-202115) and whitening effect (see JP-A-9-30946). These four kinds of plants are testosterone 5α-reductase. It is not known to have an inhibitory action and an androgen receptor binding inhibitory action.

  Moreover, although the details of the testosterone 5α-reductase inhibitor and the androgen receptor binding inhibitor obtained from these plants are unknown, both inhibitory activities are observed in the plant extracts obtained from the above plants using various solvents. . The plant extract according to the present invention includes water, various aliphatic lower alcohols such as ethanol and isopropanol, hydrophilic organic solvents such as 1,3-butylene glycol, ethylene glycol, glycerin and isopropylene glycol, and these various hydrophilic organic solvents. The plant extract exhibits high testosterone 5α-reductase inhibitory activity and androgen receptor binding inhibitory activity. In addition, these aqueous solvents are easy to handle and can be extracted relatively easily.

  These extraction target plants are generally used after each part is dried and then chopped, chopped, or pulverized. At this time, the extraction method and extraction conditions are not particularly limited, but preferably immersed in the extraction solvent in an amount of 5 to 15 times the amount of the plant used in a weight ratio, and heated at room temperature to about 90 ° C. Under heating, elute soluble components with gentle stirring. Thereafter, the extract is filtered or centrifuged, and solid-liquid separation is performed to obtain the target extract.

  The extract thus obtained can be used as it is as the testosterone 5α-reductase inhibitor and androgen receptor binding inhibitor or anti-androgen hormone agent according to the present invention. It is also possible to use it as a dry extract by further drying to a solid or using a method such as spray drying. Moreover, you may give a simple refinement | purification process as needed in the range which does not inhibit the said activity. Needless to say, this extract and dried extract can be used as they are, or various excipients such as starch and lactose can be added.

  In the present invention, the plant extract obtained as described above may be used alone or in combination of two or more kinds, or at the time of extraction, the above plant may be extracted by using two or more kinds. Of course.

  The testosterone 5α-reductase inhibitor according to the present invention thus obtained is a variety of factors resulting from excessive activity of testosterone 5α-reductase, excessive secretion of testosterone, such as male pattern baldness, hirsutism, seborrhea, acne, prostatic hypertrophy. Can be used for symptoms. The androgen receptor binding inhibitor according to the present invention is also used for various symptoms caused by binding of 5α-DHT to the androgen receptor, such as androgenetic baldness, hirsutism, seborrhea, acne and benign prostatic hyperplasia. . That is, the anti-androgen agent according to the present invention is used for all purposes which are meaningful in inhibiting the action of testosterone 5α-reductase and / or inhibiting the binding of 5α-DHT to the androgen receptor.

  In addition, these plant extracts may be used alone or in a known manner by formulating general plant extracts, or by using appropriate auxiliaries, for skin preparations, oral liquids, internal solids, injections, suppositories. It is made into various preparations such as pills and can be widely used as a component of any external preparation for skin, cosmetics, quasi-drugs, pharmaceuticals, etc., especially hair restoration cosmetics, prostate hypertrophy inhibitors and health supplements Is suitable. As the prostatic hypertrophy inhibitor, tablets, capsules, powders, liquids and the like can be used. As hair nourishing cosmetics, for example, hair tonics, hair creams, hair liquids, hair lotions, pomades, hair shampoos, hair rinses, creams, lotions, emulsions, packs, ointments, bath preparations, etc. are possible. It is. Examples of health supplements include soft drinks, Japanese and Western confectionery such as candy and chewing gum, dairy products, and other livestock processed products.

  The blending amount of the plant extract in the preparation may be appropriately examined in consideration of the purpose of use, sex, symptoms, etc., but the amount of the plant extract is about 0.005 to 10% by weight. In addition, the above plants have been used as medicinal plants since ancient times, and their safety has been confirmed, and can be used without side effects in the above-mentioned pharmaceuticals and cosmetics using these plant extracts. Is. In addition, it cannot be overemphasized that you may mix | blend 1 type or 2 types or more of 4 types of plant extracts which concern on this invention.

  Further, the hair nourishing cosmetic and the prostate hypertrophy inhibitor and the health supplement according to the present invention may be used as long as they do not interfere with the testosterone 5α-reductase inhibitory action or the androgen receptor binding inhibitory action. Various main agents and other optional auxiliaries usually used in the production of pharmaceuticals and health supplements can be used.

  Specific examples of these hair nourishing cosmetics, prostate hypertrophy inhibitors and health supplements that can be used in combination are as follows. As astringents, citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof, aluminum chloride, potassium aluminum sulfate, allantochlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenolsulfonate, zinc sulfate, diu extract, age extracts, Clam melamine extract, Gentian pepper extract, tea catechins, proanthocyanidins, diatomaceous earth extract, nettle extract, hypericum extract, diaper extract, cornflower extract, horsetail extract, kizuta extract, cucumber extract, maronier extract, salvia extract, melissa extract, tamarind husk extract, etc. Can be mentioned.

  Further, as bactericidal / antibacterial agents, benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, alkyldiaminoethylglycine chloride solution, chlorhexidine hydrochloride, orthophenylphenol, photosensitizer 101, photosensitizer No. 201, salicylic acid, sodium salicylate, sorbic acid, halocarban, resorcin, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan decomposed product, jellyfish extract, cucumber extract, enmiso extract, loquat extract, walrus extract, hop extract, Yucca extract, aloe extract, cinnamon extract, gadget extract, oil-soluble licorice extract (liquorice hydrophobic flavonoids, glabrizine, glabrene, lyco Rukon A), and the like.

  Further, as UV absorbers, β-isopropylfuranone derivatives, urocanic acid, ethyl urocanate, oxybenzone, oxybenzonesulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, cinoxalate, methyl diisopropylcinnamate, octyl methoxycinnamate, paraamino Glyceryl benzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, butylmethoxydibenzoylmethane, titanium oxide, β-carotene, γ-oryzanol, rice bran extract, aloe extract, birch extract , White birch extract, chamomile extract, white squirrel extract, hawthorn extract, henna extract, butterfly Extract, horse chestnut extract, ginkgo leaf extract, chamomile extract, oil-soluble licorice extract and the like.

  Furthermore, as a moisturizer, serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin heparin, glycerophospholipid, sphingophospholipid, glycosphingolipid, linoleic acid or esters thereof , Eicosapentaenoic acid or its esters, pectin, algae colloid, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil , Soybean phospholipid, γ-oryzanol, Bellows oyster extract, Yokuinin extract, Giant extract, Taisou extract, Gypsophila extract, Kidachi aloe extract, Burdock extract, Maronnier extract, Mannen extract, Arnica extract Peach leaf extract, wheat bran, rice bran, and the like.

  Further, as a cell activator, placental extract, riboflavin or derivative thereof, pyridoxine or derivative thereof, nicotinic acid or derivative thereof, pantothenic acid or derivative thereof, α-tocopherol or derivative thereof, arnica extract, carrot extract, rapeseed extract, Ezoukogi extract, loofah extract (saponin), sicon extract, white birch extract, agate extract, button pi extract, peonies extract, mugwort extract, safflower extract, ashitaba extract, loquat leaf extract, abalone extract, saxifrage extract, japonica extract, salvia extract, garlic An extract, a mannen wax extract, etc. are mentioned.

  Anti-inflammatory / antiallergic agents include azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or its derivatives, glycyrrhetinic acid or its derivatives, photosensitizer 301, photosensitizer 401, diphenhydramine hydrochloride, Tranexamic acid or its derivatives, adenosine phosphate, estradiol, eslon, ethinyl estradiol, cortisone, hydrocortisone, prednisone, progesterone, corticosterone, arnica extract, inchinkou extract, sanshishi extract, jujube extract, licorice extract, pearl millet extract, mugwort extract , Warmoko extract, Gentian extract, Psycho extract, Senkyu extract, Bow-fu extract, Seiryogiri extract Coptis extract, mint extract, horse chestnut extract, Scutellaria root extract, and the like.

  Antioxidant and active oxygen scavengers include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, melissa extract, oxon extract, agetsu extract, loquat leaf extract, hop extract , Hamamelis extract, Peonies extract, Sage extract, Kina extract, Chamomile extract, Eucalyptus extract, Perilla extract, Ginkgo biloba extract, Thyme extract, Callamon extract, Caraway extract, Nutmeg extract, Mace extract, Laurel extract, Clove extract, Turmeric extract, Yanagita extract Etc.

  Furthermore, the following can be used as auxiliaries: fats and oils such as soybean oil, linseed oil, tung oil, sesame oil, nuka oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, coconut oil, almond Oil, castor oil, peanut oil, cacao oil, owl, palm oil, palm kernel oil, beef tallow, mink oil, egg yolk oil, jojoba oil, evening primrose oil, horse oil and the like.

  Examples of waxes include carnauba wax, candelilla wax, beeswax, white beeswax, whale wax, serax, and lanolin.

  Examples of hydrocarbons include liquid paraffin, petrolatum, microcrystalline wax, ceresin, squalane, and polyethylene powder.

  Examples of fatty acids include stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hebenic acid, lanolinic acid, oleic acid, undecylenic acid, and isostearic acid.

  Further, as alcohols, lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol, hexadecyl alcohol, 2-octyldodecanol, glycerin, sorbitol, propylene glycol, 1,3-butylene glycol, ethylene Examples include glycols and polymers thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol, and the like.

  Further, as esters, decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, propylene glycol dioleate, diethyl phthalate, Examples include propylene glycol monostearate, ethylene glycol monostearate, glyceryl monostearate, glyceryl trimyristate, lanolin acetate, cetyl lactate, and the like.

  Examples of the surfactant include an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and a nonionic surfactant.

  Examples of the fragrance include menthol, carvone, eugenol, anethole, mint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil, and other oily fragrances.

  Further, the testosterone 5α-reductase inhibitor, androgen receptor binding inhibitor and anti-androgen hormone agent according to the present invention are also added to food for pet animals such as dog food and livestock for the purpose of preventing and treating hair loss. be able to.

Examples of the present invention will be described below, and the present invention will be described in more detail.
Example 1
1000 g of the extraction solvent shown in Table 1 is added to 100 g of each dried product (red bean cedar, strawberry, hood umbrella, chinshin lotus), heated and extracted with a reflux extractor for 2 hours, and filtered while hot. . The same extraction process was further performed on the residue. Thereafter, the obtained extracts were combined, concentrated under reduced pressure, and further dried to obtain various plant extracts. The yield for each example is shown in Table 1.

[Testosterone 5α-reductase inhibitory action]
The testosterone 5α-reductase inhibitory effect was tested on the 50 v / v% ethanol extract of red bean cedar, cocoon, hooded bonito, and shinshin lotus obtained in the Examples by the following test method.

(Test method)
Testosterone (Tokyo Kasei Co., Ltd.) (4.2 mg) was dissolved in propylene glycol (1 ml), and 20 μl of NADPH (Oriental Yeast Co., Ltd.) was dissolved to a concentration of 1 mg / ml in a 5 mM Tris-HCl buffer ( pH 7.2) Add 825 μl and mix. Furthermore, 75 μl of S-9 (rat liver homogenate: manufactured by Oriental Yeast Co., Ltd.) was added to 80 μl of each sample ethanol solution or 50 v / v% ethanol solution and distilled water, mixed, and incubated at 37 ° C. for 30 minutes. To do. Thereafter, 1 ml of methylene chloride is added to stop the reaction and shaken vigorously. Thereafter, the mixture is centrifuged, the methylene chloride layer is separated, and reaction products such as dihydroxytestosterone and androstanediol, which are reaction products, are quantified by gas chromatography. Separately, as a control, the same amount of sample solvent (80 μl) was used instead of the sample solution, and the reaction product was quantified in the same manner. The ratio of the amount of reaction product (total peak area amount) at the time of sample addition to the amount of reaction product (total peak area amount) in control was calculated to determine the testosterone 5α-reductase activity inhibition rate (%).

In this way, the sample concentration at the time of sample addition was variously changed to determine the testosterone 5α-reductase activity inhibition rate (%) at each concentration, and the sample concentration IC ₅₀ that inhibits the enzyme activity by 50% was obtained from the result by interpolation. Asked. The results are shown in Table 2.

[Androgen receptor binding inhibitory action]
The androgen receptor binding inhibitory action of each 50% v / v ethanol extract of red bean cedar obtained in the Examples, each of the ethanol extracts of persimmons, hooded straws and centripetal lotus was tested by the following test method.

(Test method)
96-well microplate of androgen-dependent mouse breast cancer cells SC-3 cells at a cell density of 1.0 × 10 ⁴ cells / well / 100 μl using MEM medium containing 2% FBS (hereinafter abbreviated as MEM-2 medium). And cultured at 37 ° C. under 5% CO ₂ -95% air. After 24 hours, the medium was replaced with a sample and 0.5% BSA-containing HamF12 + MEM medium (hereinafter abbreviated as HMB medium) to which DHT was added to a concentration of 10 ⁻⁹ molar, and cultured for 48 hours. Thereafter, the medium was replaced with MEM-2 medium containing 0.97 mM MTT, and after culturing for 2 hours, the medium was replaced with isopropanol to extract blue formazan produced in the cells. With respect to isopropanol containing eluted blue formazan, the absorbance As1 at 570 nm where the absorption maximum of blue formazan is present is measured. In order to correct the influence of the adherent cells, the absorbance at 650 nm is also measured at the same time, and the difference between the two absorbances is set to a value proportional to the amount of blue formazan produced (absorbance As0, Ab1, Ab0 in the following formula for calculating the binding inhibition rate). Is the corrected absorbance.) Next, only the sample is added instead of DHT and the above-described culture is performed, and the absorbance As0 for correction is measured. In addition, culture was carried out in an HMB medium to which no sample was added, and the absorbance Ab1 when DHT was added and the absorbance Ab0 when DHT was not added were measured, and the androgen receptor binding inhibition rate (%) was determined by the following formula. It was.
Androgen receptor binding inhibition rate (%) = {1- (As1-As0) / (Ab1-Ab0)} × 100

The inhibition rate is measured by changing the concentration of the sample solution step by step, and the androgen receptor binding inhibition rate (%) at each concentration is obtained, and the androgen binding is inhibited by 50% from the result by interpolation. The sample concentration IC ₅₀ was determined. The results are shown in Table 3.

(Hair loss inhibiting action)
About the hair nourishing cosmetic containing the koji ethanol extract obtained in the examples, the hair loss inhibiting action was tested by the following method.

(Test method)
Ten men aged 30 to 45 years with symptoms of androgenetic alopecia and cranial seborrheic dermatitis are divided into two groups, one group for each group and the other group for each group. Use 50% v / v ethanol aqueous solution containing 0.2% w / w (solid content) of salmon ethanol extract twice a day for 3 weeks instead of the usual hair tonic in the morning and evening. I got it. In the unused group, a 50 v / v% aqueous ethanol solution was used under the same conditions in place of the normally used hair tonic. Everyone was washed on the test start date and the test end date under the same conditions, and the number of hair removal at that time was measured. In order to adjust the conditions, the hair was washed under the same conditions 24 hours before washing for measuring the number of hair removal. The test results are shown in Table 4.

  In addition, in the above use test, no irritation or sensitization to the skin or scalp was observed by using a 50 v / v% ethanol aqueous solution containing the ethanol extract of persimmon according to the examples of the present invention, and its side effects were also observed. There wasn't. Furthermore, as shown in Table 4, a clear decrease was observed in the hair loss rate, and it was confirmed that hair loss was well prevented.

[Formulation example]
Next, when the following formulation example was manufactured using the plant extract obtained above, a favorable formulation could be obtained in any formulation example.

(Formulation Example 1 Tablet)
The brine extract according to Example 1 0.2 (parts by weight)
Lactose 95.0
Magnesium stearate 0.01
Coloring agent Slight amount of perfume Appropriate amount Based on the above component amounts, a tablet which is a prostate hypertrophy inhibitor according to the present invention was manufactured by a conventional method for tablet production.

(Formulation Example 2 Hair Tonic)
Purified water 70.0 (parts by weight)
Top umbrella rice bran ethanol extract according to Example 1 0.2
Pyridoxine hydrochloride 0.1
Resorcin 0.01
D-pantothenyl alcohol 0.1
Dipotassium glycyrrhizinate 0.1
Assembly extract liquid ET 0.2
l-Menthol 0.05
1,3-butylene glycol 4.0
Carrot extract 0.5
Cousin extract 0.3
Ethanol 25.0
Perfume Appropriate A hair tonic, which is a hair nourishing cosmetic according to the present invention, was manufactured based on the above component amounts and processed according to a conventional method of hair tonic manufacture.

(Formulation Example 3 Hair Lotion)
50% v / v ethanol extract 0.1% by weight according to Example 1
1,3-butylene glycol 6.0
Ethanol 8.0
Polyoxyethylene hydrogenated castor oil (40E.O.) 1.0
Polyoxysorbitan monostearate (20E.O.) 1.5
Stearyl glycyrrhetinate 0.2
Enmezo extract 0.5
Tocopherol acetate 0.05
Methyl paraoxybenzoate 0.1
Phenoxyethanol 0.3
Fragrance 0.05
Total amount of purified water remaining 100.0
Based on the said component content, it processed by the conventional method of hair lotion manufacture, and manufactured the hair lotion which is the hair nourishing cosmetics based on this invention.

(Formulation example 4 hair restorer)
Red soybean cedar 50 v / v% ethanol extract 0.5 according to Example 1 (parts by weight)
Hinokitiol 0.1
Glycyrrhetinic acid 0.1
Cephalanthin 0.02
Polyoxyethylene hydrogenated castor oil (20E.O.) 1.5
1,3-butylene glycol 3.0
Ethanol 60.0
Tocopherol acetate 0.1
Assembly extract 0.3
Total amount of purified water remaining 100.0
Based on the said component content, it processed by the normal method of the hair restorer, and the hair restorer which is the hair nourishing cosmetics based on this invention was manufactured.

(Formulation Example 5 Shampoo)
Centrifugal lotus 50 v / v% ethanol extract according to Example 1 0.05 (parts by weight)
The brine extract according to Example 1 0.15
Polyoxyethylene lauryl sulfate
Triethanolamine salt 14.0
Ethylene glycol distearate 2.0
Lauryldimethylaminoacetic acid betaine 4.0
Lauric acid diethanolamide 5.0
Glycerin 2.0
Keratin hydrolyzate 3.0
Mukuroji extract 0.2
Twilight extract 0.5
Oat extract 0.3
Rosemary extract 0.5
Paraoxybenzoic acid 0.1
Fragrance 0.05
Total amount of purified water remaining 100.0
Based on the said component content, it processed by the normal method of the shampoo, and manufactured the shampoo which is the hair nourishing cosmetics based on this invention.

(Formulation Example 6 Lotion)
Purified water 70.0 (parts by weight)
Red bean cedar 50 v / v% ethanol extract according to Example 1 0.2
Dipotassium glycyrrhizinate 0.2
1,3-butylene glycol 4.0
Oleyl alcohol 4.0
Polyoxyethylene sorbitan monolaurate (20E.O.) 1.5
Polyoxyethylene lauryl ether (20E.O.) 0.5
Ethanol 15.0
Preservative Appropriate amount of perfume Appropriate amount Based on the above component amounts, a lotion, which is an anti-androgenic agent according to the present invention, was prepared by a conventional method for producing lotions.

(Formulation Example 7 cream)
Stearyl glycyrrhetinate 0.1 (parts by weight)
Amber ethanol extract according to Example 1 0.2
Bead wax 10.0
Cetanol 5.0
Hydrophilic lanolin 8.0
Squalane 35.5
Glyceryl monostearate 2.0
γ-oryzanol 0.05
Purified water 40.0
Polyoxyethylene sorbitan monolaurate (20E.O.) 2.0
Polyethylene glycol 0.5
Preservative Appropriate amount of perfume Appropriate amount A cream which is an anti-androgenic agent according to the present invention was manufactured based on the above component amounts by a conventional method for producing cream.

(Formulation Example 8)
Sucrose 70 (parts by weight)
Minamata 30
Citric acid 1
Fragrance 0.1
Top Umbrella Ethanol Extract according to Example 1 0.01
Based on the said component content, it processed by the normal method of koji manufacture, and koji which is a health supplement based on this invention was manufactured.

(Formulation example 9 chewing gum)
Chewing gum base 70 (parts by weight)
Sucrose 54
Minamata 20
Softener 4
Perfume (mint oil) 1
Red bean cedar 50 v / v% ethanol extract 1 according to Example 1
The chewing gum which is a health supplement based on this invention was manufactured by processing by the conventional method of chewing gum manufacture based on the said component content.

  In addition, the hair nourishing cosmetics and the like, which are preparation examples of the present invention, all showed good storage stability.

Claims (5)

A testosterone 5α-reductase inhibitor comprising an extract from bird cage and / or hood umbrella. An androgen receptor binding inhibitor comprising an extract from birdcage and / or hood umbrella. An anti-androgen agent, which is an extract from birdcage and / or hood umbrella and has testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action.   A hair nourishing cosmetic comprising the testosterone 5α-reductase inhibitor according to claim 1 and / or the androgen receptor binding inhibitor according to claim 2.   A prostatic hypertrophy inhibitor comprising the testosterone 5α-reductase inhibitor according to claim 1 and / or the androgen receptor binding inhibitor according to claim 2.