JP2002179584A - TESTOSTERONE 5alpha-REDUCTASE INHIBITOR AND ANDROGEN RECEPTOR BINDING INHIBITOR, HAIR AGENT AND SKIN COSMETIC - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a novel compound excellent in testosterone 5α-reductase- inhibiting action and androgen receptor binding-inhibiting action, effective for preventing depilation and in hair generating and growing effects and excellent in safety. SOLUTION: A testosterone 5α-reductase inhibitor and/or an androgen receptor binding inhibitor relating to this invention are obtained by using one species or two or more species of Chiraito, preferably its above-ground part, Jamane mandro, preferably its bark, Timur, preferably its fruit, Pashanbhed, preferably its root and Padamchal, preferably its root, extracting them with various solvents such as water, ethanol, acetone, glycerol and the like. Further, various hair cosmetics such as a hair tonic, a hair shampoo, a lotion and the like and skin cosmetics are obtained by using the testosterone 5α-reductase inhibitor or the androgen receptor binding inhibitor.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to testosterone 5α- which reduces testosterone to 5α-dehydrotestosterone (5α-DHT), which is the active form of testosterone.
A novel and safe testosterone 5α-reductase inhibitor that inhibits the action of reductase, a novel and safe androgen receptor binding inhibitor that inhibits binding of androgen to its receptor, and containing them The present invention relates to a novel hair preparation and skin cosmetic.

[0002]

BACKGROUND OF THE INVENTION Physiological signs such as male pattern baldness, hirsutism, and seborrhea are known to be based on increased androgen stimulation due to excessive accumulation of androgens in the metabolic system. Have been. In some organs such as hair root and sebaceous gland, the main body of androgen activity is 5α-
DHT, which is known to be produced by the reduction of testosterone by testosterone 5α-reductase.

[0003] 5α-DHT binds to a receptor and exerts an action as an androgen. While androgens are important hormones, their excessive action can cause a variety of undesirable symptoms, such as male pattern baldness, trichomes, and seborrhea, as described above. As a means for improving these symptoms, a method for inhibiting the production of active 5α-DHT by inhibiting the action of testosterone 5α-reductase, which reduces testosterone to 5α-DHT, and a method for inhibiting the production of 5α-DHT produced from testosterone A method in which the action is not exhibited by inhibiting the binding to the body may be considered. In these cases, due to the inhibition of testosterone 5α-reductase activity, testosterone 5α-reductase is an enzyme that reduces testosterone to active 5α-DHT, and it is considered that the above-mentioned symptoms can be dealt with by inhibiting this reductase. . Until now, inhibitors having a steroid-like structure have been synthesized and used. However, these have some undesirable hormone-like effects and have the disadvantage that the hormone-like effects are expressed as side effects. It is thought that the above-mentioned symptoms can be dealt with by inhibiting binding of androgen to a receptor (receptor) by inhibiting androgen receptor binding. As a substance effective in inhibiting the binding between 5α-DHT and an androgen receptor, extracts such as assembly, Japanese gourd, licorice, and chimpanzee have been known. But,
None of these plant extracts have yet been found to exhibit satisfactory effects as androgen receptor binding inhibitors.

[0004] On the other hand, for the purpose of preventing and treating alopecia, hair preparations containing various drugs have hitherto been known. For example, vitamins such as vitamin B group, amino acids such as methionine, vasodilators such as acetylcholine derivatives, anti-inflammatory agents such as purple root extract, female hormones such as estradiol, and skin function enhancers such as cepharanthin are compounded. ing. However, no satisfactory effect has yet been obtained.

[0005] Furthermore, all of the above-mentioned conventional drugs used in hair preparations have only one of the inhibitory effect on testosterone 5α-reductase and the inhibitory effect on androgen receptor binding, and are effective in preventing alopecia. Prophylactic treatment required the use of multiple drugs.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above-mentioned problems of the prior art, and has excellent testosterone 5α-reductase inhibitory action and androgen receptor binding inhibitory action, and prevents hair loss, hair growth and hair growth. As a result of examining various naturally-occurring substances in order to provide a novel substance that is effective for hair growth and has excellent safety, it is possible to solve this problem with extracts obtained from certain plants and mixtures thereof. The present inventors have found an effective action and have completed the present invention.

[0007]

The testosterone 5α-reductase inhibitory activity according to the present invention comprises one or more plant extracts selected from the group consisting of tilite, jamanemandro, timul, pashanhead and padamkar. It is characterized by:

[0008] The androgen receptor binding inhibitor according to the present invention is characterized in that it comprises one or more plant extracts selected from the group consisting of tilite, jamanemandro, Timur, Pashanhead and Padamkar. I have.

Furthermore, the hair preparation according to the present invention comprises the testosterone 5α-reductase inhibitor and / or
Alternatively, it is characterized by containing the androgen receptor binding inhibitor according to the present invention.

[0010] Further, the skin cosmetic composition according to the present invention comprises the testosterone 5α-reductase inhibitor according to the present invention and / or
Alternatively, it is characterized by containing the androgen receptor binding inhibitor according to the present invention.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Testosterone 5α according to the present invention
-Reductase inhibitor or androgen receptor binding inhibitor, tilite, jamanemandro, timul,
1 selected from the group consisting of Pashanhead and Padamkar
It is characterized by being composed of a seed or two or more plant extracts.

[0012] Chilite, whose scientific name is Swerti
a chirata Buch-Ham.ex.CBClarke (Fl.Br.Ind), a plant of the Gentian family, Jamanemandro (Jama
ne mandro), whose scientific name is Mahonia nepalaulensis, is a plant of the barberry family. Timur is a plant of the family Rutaceae, whose scientific name is Zanthoxylum armatum DC. Pashanbhed is a plant of the family Saxifragaceae, whose scientific name is Bergenia ciliata (Haw.) Sternb. It is. In addition, Badamkar
l), whose scientific name is Rheum emodi Wall.ex Meissn, is a plant of the Polygonaceae family.

In the present invention, each part of each of the above-mentioned plants is used. From the viewpoint of the extraction efficiency and its effects, the above-ground part is used in tilite, the bark is used in Jamanemandro, and the fruit is used in Timur. The root is preferably used in Pashan Head, and the root is preferably used in Padamkar.

The details of the testosterone 5α-reductase inhibitor and the androgen receptor binding inhibitor obtained from these plants are unknown, but the plant extracts obtained from the above plants using various solvents have both inhibitory activities. Is recognized. The plant extract according to the present invention includes water, various aliphatic lower alcohols such as methanol, ethanol and isopropanol; polar solvents such as acetone, 1,3-butylene glycol, ethylene glycol, glycerin and isopropylene glycol; and various polar solvents such as these. And a mixture of these polar solvents and water, as well as various aqueous solvents, as well as intermediate polar solvents such as chloroform and ethyl acetate. Among them, it is preferable to use an aqueous solvent such as ethanol or a mixture of ethanol and water, and these can be easily handled and the extraction operation can be relatively easily performed.

These plants to be extracted are generally used after each part is dried, and then cut, cut into pieces, or crushed. At this time, the extraction method and the extraction conditions are not particularly limited. -Elute soluble components with gentle stirring while heating. Thereafter, the extract is filtered or centrifuged, and the target extract is obtained by solid-liquid separation.

The extract thus obtained can be used as it is as the testosterone 5α-reductase inhibitor and the androgen receptor binding inhibitor according to the present invention. However, since the activity may be low in some cases, the extract is appropriately concentrated. Alternatively, for example, it can be further dried using a method such as spray drying and used as a dry extract. In addition, a simple purification treatment may be performed, if necessary, as long as the above activity is not inhibited. Needless to say, this extract or dried extract can be used as it is, or can be used by adding various excipients such as starch and lactose.

In the present invention, the plant extract obtained as described above may be used alone or in combination of two or more. Alternatively, the plant extract may be extracted using two or more of the above plants. Of course.

The testosterone 5α-reductase inhibitor according to the present invention thus obtained includes testosterone 5α-reductase such as male pattern baldness, hirsutism, seborrhea, seborrheic dermatitis and rough skin, acne, prostatic hypertrophy and the like. Overactive,
It can be used for various symptoms caused by excessive testosterone secretion. In addition, the androgen receptor binding inhibitor according to the present invention is also capable of binding 5α-DHT to an androgen receptor, such as male pattern baldness, hirsutism, seborrhea, seborrheic dermatitis, rough skin, acne, and prostatic hypertrophy. Used for various symptoms caused by

These plant extracts can be used alone or in a known manner in the preparation of general plant extracts, or with appropriate auxiliaries, for dermatological agents, internal liquids, internal solids, injections, etc. Preparations, suppositories, etc., can be widely used as constituents of any external preparation for skin, cosmetics, quasi-drugs, pharmaceuticals, etc. Regardless, it is suitable mainly as applied to the scalp. In addition, as the dosage form, for example, hair tonic, hair cream, hair liquid, hair lotion, pomade, hair shampoo, hair rinse, hair set lotion, hair spray, hair pack, cream, lotion, emulsion, pack, etc. And can be provided in any dosage form.

The amount of the plant extract in the preparation may be appropriately determined in consideration of the purpose of use, gender, symptoms, etc., but the amount of the plant extract is about 0.005 to 10% by weight. In addition, each of the above-mentioned plants has been used as a medicinal plant since ancient times, and its safety has been confirmed, and it can be used without side effects even in the above-mentioned medicines and cosmetics using these plant extracts. Things.

Further, in the present invention, as long as the testosterone 5α-reductase inhibitory action or the androgen receptor binding inhibitory action is not hindered, various main ingredients and other auxiliaries usually used in hair preparations and the like may be used. it can. Next, the following can be used as the main agent and the auxiliary agent. Bactericidal and antibacterial agents: benzoic acid, sodium benzoate, sodium paraoxybenzoate, distearylmethylammonium chloride, benzethonium chloride, alkyldiaminoethylglycine chloride, chlorhexidine hydrochloride, orthophenylphenol, photosensitive element 101, photosensitive element 201, salicylic acid , Sodium salicylate, sorbic acid, halocarban, resorcinol, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, l-menthol, chitosan, chitosan hydrolyzate, waremokou extract, kujin extract, emisorum extract, loquat extract,
Uwaurushi extract, hop extract, yucca extract, aloe extract, cinnamon extract, gajutsu extract and the like. Humectants: serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid,
Glyceroglycolipid, sphingolipid, sphingolipid, linoleic acid or its esters, eicosapentaenoic acid or its esters, pectin, alge colloid, bifidobacterium fermentation product, lactic acid bacteria fermentation product, yeast extract,
Ganoderma mycelium culture or extract thereof, wheat germ oil, avocado oil, rice haiga oil, jojoba oil, soybean phospholipid, γ-
Oryzanol, below-flower oyster extract, yokuinin extract, jiou extract, diatom extract, diatom extract,
Kidachi aloe extract, Burdock extract, Horse chestnut extract, Mannen wax extract, Altea extract, Wheat bran, Rice bran etc. Cell activator: riboflavin or a derivative thereof, pyridoxine or a derivative thereof, ascorbic acid and a derivative thereof, nicotinic acid or a derivative thereof, pantothenic acid or a derivative thereof, α-tocopherol or a derivative thereof, arnica extract, carrot extract, sanshin ginseng extract, Eleuthero extract, loofah extract, safflower extract, Ashitaba extract, loquat leaf extract, Hioki Koshi extract, Sakinokita extract, Huang Ji extract, Salvia extract, Garlic extract, Mannen wax extract and the like. Anti-inflammatory and anti-allergic agents: azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or its derivatives, glycyrrhetinic acid or its derivatives, photosensitive element 301, photosensitive element 401, diphenhydramine hydrochloride, tranexam Acid or derivatives thereof, adenosine acid, estradiol, ethinyl estradiol, cortisone, arnica extract, chinko extract, sanshishi extract, jujuya extract, horse chestnut extract, licorice extract, syrup extract, mugwort extract, waremokou extract, gentian extract, psycho extract, Senkyu extract, Boufu extract, Yarrow extract, Perilla extract, etc. Antioxidant / active oxygen scavenger: dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate,
Baicalin, baicalen, superoxide dismutase, catalase, rosemary extract, melissa extract, ogre extract, age extract, loquat leaf extract, hop extract, hamamelis extract, peony extract, sage extract, kina extract, chamomile extract, eucalyptus extract, perilla extract, Ginkgo biloba extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, laurel extract, clove extract, turmeric extract, willow extract, etc.

These are appropriately blended according to the purpose of use and the like, and their amounts are not limited. Also,
In addition to these main ingredients and auxiliaries, surfactants, oils, fragrances, dyes, preservatives, thickeners, humectants, antioxidants, etc., which are usually required for formulation, may be arbitrarily added according to the intended use. Needless to say, the use can be facilitated and the use effect can be improved.

[0023]

EXAMPLE Next, testosterone 5α of the following example was used.
-The present invention will be described in more detail based on reductase inhibitors and androgen receptor binding inhibitors, skin cosmetics and hair preparations

(Example 1) Each plant (aboveground part of chillite,
The bark of jamanedro, the fruit of Timul, the roots of Pashanhead and the roots of Padamkar) were each crushed and dried. 100 g of each of the extraction solvents shown in Table 1 was added to each, and the mixture was heated and extracted with a reflux extractor for 3 hours and filtered while hot. did. Thereafter, the obtained extract was concentrated at 40 ° C. under reduced pressure, and dried with a freeze dryer to obtain various plant extracts. Table 1 shows the yields of each example.

[0025]

[Table 1]

[Testosterone 5α-reductase inhibitory action] The chillite, jamanemandro, Timur, Pashanhead and Padamkar obtained in Example 1 had an 80 w /
The w% ethanol extract was tested for testosterone 5α-reductase inhibitory activity by the following test method.

(Test method) 4.2 mg of testosterone (manufactured by Tokyo Chemical Industry Co., Ltd.) was dissolved in 1 ml of propylene glycol, and NADPH (manufactured by Oriental Yeast Co., Ltd.) was dissolved at a concentration of 1 mg / ml in 20 μl of the solution. 825 μl of the 5 mM Tris-HCl buffer (pH 7.2) is added and mixed. Further, an ethanol solution of each sample or a 50 v / v% ethanol solution and distilled water were used,
To each μl, 75 μl of S-9 (rat liver homogenate: manufactured by Oriental Yeast Co., Ltd.) was added and mixed.
Incubate at 30 ° C for 30 minutes. Thereafter, the reaction was stopped by adding 1 ml of methylene chloride, and the mixture was vigorously shaken.
Thereafter, the mixture is centrifuged, the methylene chloride layer is separated, and the reaction products such as dihydroxytestosterone and androstanediol are quantified by gas chromatography. Separately, as a control, the same treatment (80 μl) as the sample solvent was used instead of the sample solution, and the same treatment was performed to quantify the reaction product. The ratio of the amount of reaction product (total peak area) at the time of sample addition to the amount of reaction product (total peak area) in the control was calculated, and the testosterone 5α-reductase activity inhibition rate (%) was determined.

Thus, the testosterone 5α-
The reductase activity inhibition rate (%) was determined, and the sample concentration IC _{50 that} inhibited the enzyme activity by 50% was obtained from the result by interpolation.
I asked. Table 2 shows the results.

[0029]

[Table 2]

[Androgen Receptor Binding Inhibiting Effect] The 80% w / w ethanol extract of tilite, jamanemandro, Timur, Pashanhead and Padamkar obtained in Example 1 was tested for its inhibitory effect on androgen receptor binding by the following test method. It was measured.

(Test method) Androgen-dependent mouse breast cancer cells SC-3 cells were cultured in a MEM medium containing 2% FBS (hereinafter abbreviated as MEM-2 medium) at 1.0 × 10 ⁴ cells.
The cells were seeded on a 96-well microplate at a cell density of / well / 100 μl and cultured at 37 ° C. under 5% CO ₂ -95% air. Twenty-four hours later, the sample and H containing 0.5% BSA to which DHT was added to a concentration of 1.0 × 10 ⁻⁹ mol.
The medium was replaced with amF12 ten MEM medium (hereinafter abbreviated as HMB medium), and the cells were cultured for 48 hours. Then, medium was added to 0.9
The medium was replaced with a MEM-2 medium containing 7 mM MTT, and after culturing for 2 hours, the medium was replaced with isopropanol to extract blue formazan produced in the cells. With respect to the isopropanol containing the eluted blue formazan, the absorbance As1 at 570 nm where the absorption maximum point of blue formazan was measured. In addition, in order to correct the influence of the adherent cells, the absorbance at 650 nm was also measured at the same time, and the difference between the two absorbances was used as a value proportional to the amount of blue formazan produced (absorbance As in the following formula for calculating the binding inhibition rate).
0, Ab1, Ab0 are the corrected absorbances. ).
Next, only the sample was added instead of DHT, and the above culture was performed, and the absorbance As0 for correction was measured. In addition, culturing was performed in an HMB medium to which no sample was added, and the absorbance Ab1 when DHT was added and the absorbance Ab0 when DHT was not added were measured, and the androgen receptor binding inhibition rate (%) was determined by the following equation. Was. Androgen receptor binding inhibition rate (%) = ｛1- (As1
-As0) / (Ab1-Ab0)｝ × 100 The above-mentioned inhibition rate was measured by changing the concentration of the sample solution stepwise, and the androgen receptor binding inhibition rate (%) at each concentration was determined. The sample concentration IC ₅₀ that inhibits androgen binding by 50% was determined by the interpolation method. Table 3 shows the results.

[0032]

[Table 3]

[Test of hair growth effect] C3H / HeN Slc
After practicing 60 male mice of 7 weeks of age for more than one week,
Fifty-six normal animals were selected and used in groups of seven and eight.

(Test Method) Carefully cut the back hairs of mice that had reached a telogen period at the age of 8 weeks or older into an oval shape with an electric clipper and carefully with an electric razor so as not to damage the skin. Shaving was performed. From the day after shaving, a test sample (a hair preparation) shown in the composition table in Table 4 was applied to the shaved portion at a rate of 100 μl once a day for 5 days a week, using a 200 μl tip so as not to cause irritation. did. Application was performed until the 28th day from the start of administration. During that time, observations were made daily before application, 5 days a week. The day after the final application day, the regenerated hair was shaved and the weight of the hair growth was measured. The hair growth effect was determined based on the weight of the regenerated hair. Table 5 shows the results. In addition, 80 w / w% ethanol extract was used for each plant extract, and as a comparative control, an assembly extract known to have a hair growth effect (treated in the same manner as in Example 1) was used.

[0035]

[Table 4]

[0036]

[Table 5]

As can be seen from the results of the above tests, it was confirmed that each extract exhibited extremely excellent testosterone 5α-reductase inhibitory activity and androgen receptor binding inhibitory activity. It was also confirmed that the hair preparation of the present invention also exhibited an excellent hair-growing effect.

[Preparation Examples] Next, when the following preparation examples were produced using the plant extract obtained above, good preparations could be obtained in any of the preparation examples. In addition,
In the following formulation examples, extracts using 80 w / w% ethanol and ethanol as extraction solvents were used as extracts of each plant.

(Formulation Example 1 Hair tonic) Tilite extract 0.5 (parts by weight) Resorcinol 0.01 Dipotassium glycyrrhizinate 0.1 Carrot extract 0.5 Pyridoxine hydrochloride 0.1 D-Pantothenyl alcohol 0.1 L- Menthol 0.05 1,3-butylene glycol 4.0 Assembly extract 0.2 Placenta extract 0.2 Perfume Appropriate amount Ethanol 25.0 Purified water 70.0 The above component amounts were taken to produce a hair tonic according to a conventional method.

(Formulation Example 2 Hair Lotion) Arnica Extract 0.5 (parts by weight) Jamane Mandro Extract 0.5 1,3-butylene glycol 4.0 Salicylic acid 0.2 Ginger extract 0.1 Cepharanthin 0.1 Hyaluronic acid 0.1 dl-tocopherol acetate 0.1 glycyrrhetinic acid 0.2 oleyl alcohol 4.0 Preservative qs polyoxyethylene sorbitan monolaurate (20E.O.) 1.5 polyoxyethylene lauryl ether (20E.O.) 0.5) Perfume Appropriate amount Ethanol 15.0 Purified water 70.0 The above component amounts were taken to prepare a hair lotion according to a conventional method.

(Formulation Example 3 Hair Cream) Timul Extract 0.5 (parts by weight) Stearyl Glycyrrhetinate 0.1 Chamomile Extract 0.1 Aloe Extract 0.1 Thai Soybean Extract 0.1 Pantothenic Acid 0.1 Photosensitizer 301 0.1 Beeswax 10.0 Cetanol 5.0 Hydrophilic laurin 8.0 Squalane 37.5 Glyceryl monostearate 2.0 γ-oryzanol 0.05 Polyoxyethylene sorbitan monolaurate (20E.O.) 2.0 Polyethylene glycol 5.0 Preservatives qs Perfume qs Purified water 40.0 The above component amounts were taken to produce a hair cream according to a conventional method.

(Formulation Example 4 Shampoo) Triethanolamine lauryl sulfate 5.0 (g) Polyoxyethylene (20E.O.) Sodium lauryl ether sulfate 12.0 Lauryl diethanolamide 2.0 Disodium edetate 0.1 1,3-butylene glycol 4.0 Methyl parahydroxybenzoate 0.15 Fragrance 0.05 Pashanhead extract 1.0 Purified water The total amount was 100 g. The above component amounts were taken, and a shampoo was produced according to a conventional method.

(Formulation Example 5 rinse) Stearyltrimethylammonium chloride 2.0 (g) Cetostearyl alcohol 2.0 Polyoxyethylene lanolin ether (20E.O.) 3.0 Methyl parahydroxybenzoate 0.15 Propylene glycol 5. 0 Perfume 0.05 Padamcal extract 2.0 Purified water The total amount was set to 100 g. The above component amounts were taken, and a rinse was produced according to a conventional method.

(Formulation Example 6 Lotion) Arnica extract BG 0.5 (parts by weight) Oil-soluble licorice extract PT (40) 0.1 Kuddin extract BG 0.2 1,3-butylene glycol 4.0 oleyl Alcohol 4.0 Preservative Qs tilite extract 0.5 Jamane mandro extract 0.5 Polyoxyethylene sorbitan monolaurate (20E.O.) 1.5 Polyoxyethylene lauryl ether (20E.O.) 0.5 Perfume Appropriate amount Ethanol 15.0 Purified water 70.0 The above component amounts were taken and a lotion was produced according to a conventional method.

Formulation Example 7 Cream Stearyl glycyrrhetinate 0.1 (parts by weight) beeswax 10.0 cetanol 5.0 hydrophilic lanolin 8.0 squalane 32.5 glyceryl monostearate 2.0 γ-oryzanol 0 .05 Timur extract 0.5 Padamcal extract 2.0 Vitamin E acetate 0.2 Polyoxyethylene sorbitan monolaurate (20E.O.) 2.0 Polyethylene glycol 5.0 Preservatives Appropriate amount Perfume Appropriate amount Purified water 34 0.0 The above component amounts were taken, and a cream was produced according to a conventional method.

[0046]

The testosterone 5α-reductase inhibitor and the androgen receptor binding inhibitor of the present invention are safe and have excellent testosterone 5α-reductase inhibitory activity and androgen receptor binding inhibitory activity.
Therefore, by using such an inhibitor, a hair preparation that is effective and effective in improving various unfavorable symptoms such as male pattern baldness, hair loss, seborrhea and the like without blending various drugs. , Skin cosmetics and the like can be provided.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/00 A61K 7/00 K 7/06 7/06 7/48 7/48 A61P 13/08 A61P 13 / 08 17/00 17/00 17/08 17/08 17/14 17/14 43/00 111 43/00 111 (72) Inventor Yoshihito Kawashima 14703 Mukoto-cho, Onomichi-shi, Hiroshima 10 Maruzen Pharmaceutical Co., Ltd. (72) Inventor Naoko Kishida 14703, Mukoto-cho, Onomichi-shi, Hiroshima F-term within Maruzen Pharmaceutical Co., Ltd. AD532 AD662 CC05 CC33 CC37 CC38 CC39 DD23 DD27 DD31 EE13 EE22 4C088 AB43 AB62 AB63 AB66 AB67 AC02 AC04 AC06 AC11 BA08 BA09 BA10 CA03 CA11 MA07 MA63 NA14 ZA81 ZA89 ZA92 ZC03 ZC20

Claims (4)

[Claims] Claims: 1. Chilite, Jamanemandro, Timul,
1 selected from the group consisting of Pashanhead and Padamkar
A testosterone 5α-reductase inhibitor comprising a species or two or more plant extracts. 2. Chillite, Jamanemandro, Timul,
1 selected from the group consisting of Pashanhead and Padamkar
An androgen receptor binding inhibitor comprising a species or two or more plant extracts. 3. A hair preparation comprising the testosterone 5α-reductase inhibitor according to claim 1 and / or the androgen receptor binding inhibitor according to claim 2. 4. A skin cosmetic comprising the testosterone 5α-reductase inhibitor according to claim 1 and / or the androgen receptor binding inhibitor according to claim 2.