JP5068388B2 - 累積時間分解発光二次元ゲル電気泳動法 - Google Patents

累積時間分解発光二次元ゲル電気泳動法 Download PDF

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JP5068388B2
JP5068388B2 JP2011509961A JP2011509961A JP5068388B2 JP 5068388 B2 JP5068388 B2 JP 5068388B2 JP 2011509961 A JP2011509961 A JP 2011509961A JP 2011509961 A JP2011509961 A JP 2011509961A JP 5068388 B2 JP5068388 B2 JP 5068388B2
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fluorescence
gel
image acquisition
protein
dge
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JP2011521247A (ja
JP2011521247A5 (enExample
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ウィーロック、オーサ
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ヒカリ バイオ エービー
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G01N27/44778—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis on a common gel carrier, i.e. 2D gel electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2011509961A 2008-05-19 2009-05-19 累積時間分解発光二次元ゲル電気泳動法 Active JP5068388B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US5430008P 2008-05-19 2008-05-19
US61/054,300 2008-05-19
US12/454,334 US8218878B2 (en) 2008-05-19 2009-05-15 Cumulative time-resolved emission two-dimensional gel electrophoresis
US12/454,334 2009-05-15
PCT/EP2009/056072 WO2009141349A1 (en) 2008-05-19 2009-05-19 Cumulative time-resolved emission two-dimensional gel electrophoresis

Publications (3)

Publication Number Publication Date
JP2011521247A JP2011521247A (ja) 2011-07-21
JP2011521247A5 JP2011521247A5 (enExample) 2012-07-26
JP5068388B2 true JP5068388B2 (ja) 2012-11-07

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JP2011509961A Active JP5068388B2 (ja) 2008-05-19 2009-05-19 累積時間分解発光二次元ゲル電気泳動法

Country Status (7)

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US (1) US8218878B2 (enExample)
EP (1) EP2283350B1 (enExample)
JP (1) JP5068388B2 (enExample)
CN (1) CN102037352B (enExample)
AT (1) ATE545017T1 (enExample)
ES (1) ES2382113T3 (enExample)
WO (1) WO2009141349A1 (enExample)

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Publication number Priority date Publication date Assignee Title
DE102009005953A1 (de) * 2009-01-19 2010-07-22 Universität Tübingen Verfahren und System zur Charakterisierung einer Probe mittels bildgebender Fluoreszenzmikroskopie
US9797910B2 (en) 2013-06-19 2017-10-24 Merck Sharp & Dohme Corp. Assay for determining endogenous levels of analyte in vivo
FR3008186B1 (fr) * 2013-07-04 2016-12-09 Commissariat Energie Atomique Procede de detection non destructif d'interactions proteines uranium sur gels d'electrophorese
KR101758114B1 (ko) 2015-11-30 2017-07-14 재단법인 오송첨단의료산업진흥재단 에너지 전이에 따른 형광 수명을 측정하는 방법
WO2019059379A1 (ja) * 2017-09-21 2019-03-28 国立大学法人北海道大学 生体検査装置及び生体検査方法
CN109060746B (zh) * 2018-08-21 2021-12-21 复旦大学 一种比率型荧光检测方法及应用
GB201918336D0 (en) * 2019-12-12 2020-01-29 Ucl Business Ltd Time-resolved method of protein analysis
CN112070678B (zh) * 2020-08-10 2023-04-11 华东交通大学 一种批量蛋白质印迹膜条倾斜矫正和分割方法及系统
CN112861986B (zh) * 2021-03-02 2022-04-22 广东工业大学 一种基于卷积神经网络的血脂亚组分含量检测方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6147198A (en) * 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
JP2524243B2 (ja) * 1990-04-12 1996-08-14 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パタ―ン読み取り装置
JP3012794B2 (ja) * 1995-10-02 2000-02-28 日立ソフトウエアエンジニアリング株式会社 スキャナ装置
JPH1194743A (ja) * 1997-09-18 1999-04-09 Hitachi Software Eng Co Ltd 蛍光パターン読み取り装置
JP2002168787A (ja) * 2000-12-04 2002-06-14 Fuji Photo Film Co Ltd 画像読み取り方法および装置

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Publication number Publication date
US8218878B2 (en) 2012-07-10
ES2382113T3 (es) 2012-06-05
US20090316992A1 (en) 2009-12-24
JP2011521247A (ja) 2011-07-21
WO2009141349A1 (en) 2009-11-26
CN102037352B (zh) 2015-03-25
ATE545017T1 (de) 2012-02-15
CN102037352A (zh) 2011-04-27
EP2283350B1 (en) 2012-02-08
EP2283350A1 (en) 2011-02-16

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