CN102037352B - 累积的时间分辨发射双向凝胶电泳 - Google Patents

累积的时间分辨发射双向凝胶电泳 Download PDF

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Publication number
CN102037352B
CN102037352B CN200980117868.7A CN200980117868A CN102037352B CN 102037352 B CN102037352 B CN 102037352B CN 200980117868 A CN200980117868 A CN 200980117868A CN 102037352 B CN102037352 B CN 102037352B
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fluorescence
gel
protein
dge
detection
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Chinese (zh)
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CN102037352A (zh
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奥萨·惠洛克
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HiKari Bio AB
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HiKari Bio AB
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G01N27/44778—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis on a common gel carrier, i.e. 2D gel electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN200980117868.7A 2008-05-19 2009-05-19 累积的时间分辨发射双向凝胶电泳 Active CN102037352B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US5430008P 2008-05-19 2008-05-19
US61/054,300 2008-05-19
US12/454,334 US8218878B2 (en) 2008-05-19 2009-05-15 Cumulative time-resolved emission two-dimensional gel electrophoresis
US12/454,334 2009-05-15
PCT/EP2009/056072 WO2009141349A1 (en) 2008-05-19 2009-05-19 Cumulative time-resolved emission two-dimensional gel electrophoresis

Publications (2)

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CN102037352A CN102037352A (zh) 2011-04-27
CN102037352B true CN102037352B (zh) 2015-03-25

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Country Link
US (1) US8218878B2 (enExample)
EP (1) EP2283350B1 (enExample)
JP (1) JP5068388B2 (enExample)
CN (1) CN102037352B (enExample)
AT (1) ATE545017T1 (enExample)
ES (1) ES2382113T3 (enExample)
WO (1) WO2009141349A1 (enExample)

Families Citing this family (9)

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DE102009005953A1 (de) * 2009-01-19 2010-07-22 Universität Tübingen Verfahren und System zur Charakterisierung einer Probe mittels bildgebender Fluoreszenzmikroskopie
US9797910B2 (en) 2013-06-19 2017-10-24 Merck Sharp & Dohme Corp. Assay for determining endogenous levels of analyte in vivo
FR3008186B1 (fr) * 2013-07-04 2016-12-09 Commissariat Energie Atomique Procede de detection non destructif d'interactions proteines uranium sur gels d'electrophorese
KR101758114B1 (ko) 2015-11-30 2017-07-14 재단법인 오송첨단의료산업진흥재단 에너지 전이에 따른 형광 수명을 측정하는 방법
WO2019059379A1 (ja) * 2017-09-21 2019-03-28 国立大学法人北海道大学 生体検査装置及び生体検査方法
CN109060746B (zh) * 2018-08-21 2021-12-21 复旦大学 一种比率型荧光检测方法及应用
GB201918336D0 (en) * 2019-12-12 2020-01-29 Ucl Business Ltd Time-resolved method of protein analysis
CN112070678B (zh) * 2020-08-10 2023-04-11 华东交通大学 一种批量蛋白质印迹膜条倾斜矫正和分割方法及系统
CN112861986B (zh) * 2021-03-02 2022-04-22 广东工业大学 一种基于卷积神经网络的血脂亚组分含量检测方法

Citations (1)

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Publication number Priority date Publication date Assignee Title
US20020066866A1 (en) * 2000-12-04 2002-06-06 Fuji Photo Film Co., Ltd. Image reading method and apparatus

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Publication number Priority date Publication date Assignee Title
US6147198A (en) * 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
JP2524243B2 (ja) * 1990-04-12 1996-08-14 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パタ―ン読み取り装置
JP3012794B2 (ja) * 1995-10-02 2000-02-28 日立ソフトウエアエンジニアリング株式会社 スキャナ装置
JPH1194743A (ja) * 1997-09-18 1999-04-09 Hitachi Software Eng Co Ltd 蛍光パターン読み取り装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020066866A1 (en) * 2000-12-04 2002-06-06 Fuji Photo Film Co., Ltd. Image reading method and apparatus

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A Fast Global Fitting Algorithm for Fluorescence Lifetime Imaging Microscopy Based on Image Segmentation;S. Pelet等;《Biophysical Journal》;20041031;第87卷(第4期);第2807-2809、第2813-2815页 *
Measurement of Protein Sulfhydryls in Response to Cellular Oxidative Stress Using Gel Electrophoresis and Multiplexed Fluorescent Imaging Analysis;Page C.Spiess等;《Chem.Res.Toxicol.》;20080417;第21卷(第5期);第1076页 *
Time-Resolved Fluorescence Imaging of Slab Gels for Lifetime Base-Calling in DNA Sequencing Applications;Suzanne J.Lassiter等;《Analytical Chemistry》;20000410;第72卷(第21期);第5373-5382页 *

Also Published As

Publication number Publication date
US8218878B2 (en) 2012-07-10
ES2382113T3 (es) 2012-06-05
US20090316992A1 (en) 2009-12-24
JP2011521247A (ja) 2011-07-21
JP5068388B2 (ja) 2012-11-07
WO2009141349A1 (en) 2009-11-26
ATE545017T1 (de) 2012-02-15
CN102037352A (zh) 2011-04-27
EP2283350B1 (en) 2012-02-08
EP2283350A1 (en) 2011-02-16

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