JP5039307B2 - 対物レンズおよび顕微鏡 - Google Patents
対物レンズおよび顕微鏡 Download PDFInfo
- Publication number
- JP5039307B2 JP5039307B2 JP2006043796A JP2006043796A JP5039307B2 JP 5039307 B2 JP5039307 B2 JP 5039307B2 JP 2006043796 A JP2006043796 A JP 2006043796A JP 2006043796 A JP2006043796 A JP 2006043796A JP 5039307 B2 JP5039307 B2 JP 5039307B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- irradiation
- microscope
- detection
- diaphragm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001514 detection method Methods 0.000 claims description 42
- 238000005286 illumination Methods 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 16
- 210000001747 pupil Anatomy 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 description 16
- 230000005284 excitation Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 3
- 238000000204 total internal reflection microscopy Methods 0.000 description 3
- 238000007740 vapor deposition Methods 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 238000004621 scanning probe microscopy Methods 0.000 description 2
- 238000000492 total internal reflection fluorescence microscopy Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001609 comparable effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
- G02B21/084—Condensers for incident illumination only having annular illumination around the objective
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
- Lenses (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE200510009832 DE102005009832A1 (de) | 2005-03-01 | 2005-03-01 | Objektiv und Mikroskop |
| DE102005009832.0 | 2005-03-01 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006243723A JP2006243723A (ja) | 2006-09-14 |
| JP2006243723A5 JP2006243723A5 (enExample) | 2009-02-19 |
| JP5039307B2 true JP5039307B2 (ja) | 2012-10-03 |
Family
ID=36218595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006043796A Expired - Fee Related JP5039307B2 (ja) | 2005-03-01 | 2006-02-21 | 対物レンズおよび顕微鏡 |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1698929B1 (enExample) |
| JP (1) | JP5039307B2 (enExample) |
| CN (1) | CN1828357A (enExample) |
| DE (2) | DE102005009832A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7855844B2 (en) | 2007-05-17 | 2010-12-21 | Mitutoyo Corporation | Objective lens and optical measuring device |
| DE102010034122B4 (de) | 2010-08-12 | 2020-03-26 | Carl Zeiss Microscopy Gmbh | Mikroskop und Objektiv, insbesondere für die TIRF-Mikroskopie |
| JP5834638B2 (ja) * | 2011-09-02 | 2015-12-24 | 株式会社ニコン | 対物レンズユニット及びこの対物レンズユニットを有する走査型顕微鏡 |
| CN102818796B (zh) * | 2012-07-23 | 2016-01-27 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| CN102818795B (zh) * | 2012-07-23 | 2015-08-26 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| CN102818794B (zh) * | 2012-07-23 | 2016-01-27 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| DE102017110638B3 (de) * | 2017-05-16 | 2018-09-27 | Leica Microsystems Cms Gmbh | Mikroskop und Mikroskopbeleuchtungsverfahren |
| DE102017214189A1 (de) * | 2017-08-15 | 2019-02-21 | Carl Zeiss Microscopy Gmbh | Verfahren zum Betrieb einer Mikroskopieranordnung und Mikroskopieranordnung mit einem ersten Mikroskop und mindestens einem weiteren Mikroskop |
| CN109100352B (zh) * | 2018-08-30 | 2024-07-23 | 天津港东科技股份有限公司 | 显微镜多功能液体测试装置 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4671463B2 (ja) | 2000-03-24 | 2011-04-20 | オリンパス株式会社 | 照明光学系及び照明光学系を備えた顕微鏡 |
| US6597499B2 (en) * | 2001-01-25 | 2003-07-22 | Olympus Optical Co., Ltd. | Total internal reflection fluorescence microscope having a conventional white-light source |
| DE10108796A1 (de) | 2001-02-21 | 2002-09-05 | Zeiss Carl Jena Gmbh | Hochaperturiges Objektiv |
| DE10143481A1 (de) | 2001-09-05 | 2003-03-20 | Europ Lab Molekularbiolog | Mikroskop |
| JP2003098439A (ja) * | 2001-09-25 | 2003-04-03 | Olympus Optical Co Ltd | 観察切り替え可能な顕微鏡 |
| DE10217098B4 (de) | 2002-04-17 | 2004-04-15 | Carl Zeiss Jena Gmbh | Auflicht-Beleuchtungsanordnung für ein Mikroskop |
| DE10229935B4 (de) | 2002-07-04 | 2018-02-08 | Carl Zeiss Microscopy Gmbh | Mikroskopschieber zur Einkopplung von Licht in ein Mikroskop |
| DE10309269B4 (de) * | 2003-03-03 | 2005-06-02 | Till Photonics Gmbh | Vorrichtung für Totale Interne Reflexions-Mikroskopie |
| DE102004015587A1 (de) * | 2003-04-04 | 2004-11-11 | Olympus Corporation | Fluoreszenzmikroskop mit totaler interner Reflexion |
| DE10344410A1 (de) * | 2003-09-25 | 2005-04-28 | Leica Microsystems | Rastermikroskop mit evaneszenter Beleuchtung |
-
2005
- 2005-03-01 DE DE200510009832 patent/DE102005009832A1/de not_active Withdrawn
-
2006
- 2006-01-23 EP EP20060001362 patent/EP1698929B1/de not_active Not-in-force
- 2006-01-23 DE DE200650001206 patent/DE502006001206D1/de active Active
- 2006-02-21 JP JP2006043796A patent/JP5039307B2/ja not_active Expired - Fee Related
- 2006-03-01 CN CNA2006100198198A patent/CN1828357A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE502006001206D1 (de) | 2008-09-11 |
| JP2006243723A (ja) | 2006-09-14 |
| DE102005009832A1 (de) | 2006-09-07 |
| CN1828357A (zh) | 2006-09-06 |
| EP1698929B1 (de) | 2008-07-30 |
| EP1698929A1 (de) | 2006-09-06 |
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