JP5021183B2 - タンパク質固定化膜および固定化方法、ならびにバイオセンサ - Google Patents

タンパク質固定化膜および固定化方法、ならびにバイオセンサ Download PDF

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Publication number
JP5021183B2
JP5021183B2 JP2005148253A JP2005148253A JP5021183B2 JP 5021183 B2 JP5021183 B2 JP 5021183B2 JP 2005148253 A JP2005148253 A JP 2005148253A JP 2005148253 A JP2005148253 A JP 2005148253A JP 5021183 B2 JP5021183 B2 JP 5021183B2
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Prior art keywords
cygdh
immobilized
cell membrane
biosensor
layer
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Expired - Fee Related
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JP2005148253A
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English (en)
Japanese (ja)
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JP2006322889A5 (cg-RX-API-DMAC7.html
JP2006322889A (ja
Inventor
幸治 勝木
秀亮 山岡
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Arkray Inc
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Arkray Inc
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Priority to JP2005148253A priority Critical patent/JP5021183B2/ja
Application filed by Arkray Inc filed Critical Arkray Inc
Priority to US11/920,782 priority patent/US20090101499A1/en
Priority to CN2006800226213A priority patent/CN101203748B/zh
Priority to EP06746594.8A priority patent/EP1884771B1/en
Priority to PCT/JP2006/309906 priority patent/WO2006123730A1/ja
Publication of JP2006322889A publication Critical patent/JP2006322889A/ja
Publication of JP2006322889A5 publication Critical patent/JP2006322889A5/ja
Application granted granted Critical
Publication of JP5021183B2 publication Critical patent/JP5021183B2/ja
Priority to US14/850,576 priority patent/US9702843B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/80—Cytochromes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Emergency Medicine (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2005148253A 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ Expired - Fee Related JP5021183B2 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2005148253A JP5021183B2 (ja) 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ
CN2006800226213A CN101203748B (zh) 2005-05-20 2006-05-18 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器
EP06746594.8A EP1884771B1 (en) 2005-05-20 2006-05-18 Protein-immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor
PCT/JP2006/309906 WO2006123730A1 (ja) 2005-05-20 2006-05-18 タンパク質固定膜、タンパク質の固定化方法、酵素固定化電極、およびバイオセンサ
US11/920,782 US20090101499A1 (en) 2005-05-20 2006-05-18 Protein-Immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor
US14/850,576 US9702843B2 (en) 2005-05-20 2015-09-10 Biosensor incorporating protein-immobilized membrane and method of immobilizing protein in biosensor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2005148253A JP5021183B2 (ja) 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ

Publications (3)

Publication Number Publication Date
JP2006322889A JP2006322889A (ja) 2006-11-30
JP2006322889A5 JP2006322889A5 (cg-RX-API-DMAC7.html) 2008-03-06
JP5021183B2 true JP5021183B2 (ja) 2012-09-05

Family

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Family Applications (1)

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JP2005148253A Expired - Fee Related JP5021183B2 (ja) 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ

Country Status (5)

Country Link
US (2) US20090101499A1 (cg-RX-API-DMAC7.html)
EP (1) EP1884771B1 (cg-RX-API-DMAC7.html)
JP (1) JP5021183B2 (cg-RX-API-DMAC7.html)
CN (1) CN101203748B (cg-RX-API-DMAC7.html)
WO (1) WO2006123730A1 (cg-RX-API-DMAC7.html)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060091006A1 (en) * 1999-11-04 2006-05-04 Yi Wang Analyte sensor with insertion monitor, and methods
US7497940B2 (en) * 2003-09-02 2009-03-03 Koji Sode Glucose sensor and glucose level measuring apparatus
JP2008243380A (ja) * 2007-03-23 2008-10-09 Sony Corp 酵素固定化電極、燃料電池、電子機器、酵素反応利用装置および酵素固定化基体
US8268604B2 (en) * 2007-12-20 2012-09-18 Abbott Point Of Care Inc. Compositions for forming immobilized biological layers for sensing
AU2010286191B2 (en) 2008-09-17 2014-11-13 Avery Dennison Corporation Activatable adhesive, labels, and related methods
US9653006B2 (en) 2008-09-17 2017-05-16 Avery Dennison Corporation Activatable adhesive, labels, and related methods
CN102308204B (zh) * 2009-02-09 2014-07-09 爱科来株式会社 电化学传感器及其制备方法
JP5432575B2 (ja) * 2009-04-21 2014-03-05 グンゼ株式会社 バイオセンサ及びその製造方法
CN102414319A (zh) * 2009-04-30 2012-04-11 池田食研株式会社 蛋白质性电子介质
JP5665070B2 (ja) * 2009-09-25 2015-02-04 独立行政法人産業技術総合研究所 酸化還元タンパク質固定化ナノ構造電極
CN103344639B (zh) * 2013-07-11 2015-10-28 山东大学 一种快速检测尿中吡咯加合物的检测管
JP6435264B2 (ja) * 2013-08-07 2018-12-05 アークレイ株式会社 電気化学式バイオセンサを用いた物質の測定方法及び測定装置
WO2016199741A1 (ja) * 2015-06-08 2016-12-15 国立研究開発法人科学技術振興機構 高密度微小チャンバーアレイおよびこれを用いた測定方法
CN105355530B (zh) * 2015-10-30 2017-08-11 三诺生物传感股份有限公司 一种用于试条电极预处理的等离子处理装置和方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0810208A (ja) 1994-06-30 1996-01-16 Toshiba Corp 食器洗浄機
US5650062A (en) * 1995-03-17 1997-07-22 Matsushita Electric Industrial Co., Ltd. Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same
WO2001020316A1 (en) * 1999-09-13 2001-03-22 Matsushita Electric Industrial Co., Ltd. Process for producing lipid-modified enzyme and biosensor
US6458599B1 (en) 2000-02-18 2002-10-01 Aspira Biosystems, Inc. Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules
AU2001241531A1 (en) * 2000-02-18 2001-08-27 Aspira Biosystems, Inc. Compositions and methods for surface imprinting
WO2002009647A2 (en) * 2000-07-28 2002-02-07 Emory University Biological component comprising artificial membrane
JP2002055076A (ja) * 2000-09-08 2002-02-20 Nec Corp 電気化学センサ
EP1331272B1 (en) * 2000-10-31 2007-06-06 Koji Sode Novel glucose dehydrogenase and process for producing the dehydrogenase
DK2330407T3 (da) * 2001-09-14 2013-06-24 Arkray Inc Fremgangsmåde, værktøj og indretning til at måle en koncentration
KR100729307B1 (ko) * 2002-06-17 2007-06-15 아크레이 인코퍼레이티드 글루코스 탈수소효소를 이용한 글루코스 농도 측정방법 및글루코스 센서
JP4637579B2 (ja) * 2002-07-25 2011-02-23 パナソニック株式会社 電解質膜とこれを用いた膜電極接合体および燃料電池
JP4250481B2 (ja) * 2003-08-11 2009-04-08 キヤノン株式会社 多孔質構造体
US7497940B2 (en) * 2003-09-02 2009-03-03 Koji Sode Glucose sensor and glucose level measuring apparatus
WO2005030807A1 (ja) * 2003-09-30 2005-04-07 Koji Sode グルコース脱水素酵素とシトクロームとの融合蛋白質

Also Published As

Publication number Publication date
EP1884771B1 (en) 2017-12-13
EP1884771A1 (en) 2008-02-06
US9702843B2 (en) 2017-07-11
CN101203748B (zh) 2011-09-14
EP1884771A4 (en) 2011-10-19
US20150377818A1 (en) 2015-12-31
WO2006123730A1 (ja) 2006-11-23
CN101203748A (zh) 2008-06-18
US20090101499A1 (en) 2009-04-23
JP2006322889A (ja) 2006-11-30

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