CN101203748B - 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 - Google Patents

蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 Download PDF

Info

Publication number
CN101203748B
CN101203748B CN2006800226213A CN200680022621A CN101203748B CN 101203748 B CN101203748 B CN 101203748B CN 2006800226213 A CN2006800226213 A CN 2006800226213A CN 200680022621 A CN200680022621 A CN 200680022621A CN 101203748 B CN101203748 B CN 101203748B
Authority
CN
China
Prior art keywords
enzyme
immobilized
biosensor
cell membrane
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2006800226213A
Other languages
English (en)
Chinese (zh)
Other versions
CN101203748A (zh
Inventor
胜木幸治
山冈秀亮
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Inc
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Publication of CN101203748A publication Critical patent/CN101203748A/zh
Application granted granted Critical
Publication of CN101203748B publication Critical patent/CN101203748B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/80—Cytochromes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Emergency Medicine (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2006800226213A 2005-05-20 2006-05-18 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 Expired - Fee Related CN101203748B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005148253A JP5021183B2 (ja) 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ
JP148253/2005 2005-05-20
PCT/JP2006/309906 WO2006123730A1 (ja) 2005-05-20 2006-05-18 タンパク質固定膜、タンパク質の固定化方法、酵素固定化電極、およびバイオセンサ

Publications (2)

Publication Number Publication Date
CN101203748A CN101203748A (zh) 2008-06-18
CN101203748B true CN101203748B (zh) 2011-09-14

Family

ID=37431302

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2006800226213A Expired - Fee Related CN101203748B (zh) 2005-05-20 2006-05-18 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器

Country Status (5)

Country Link
US (2) US20090101499A1 (cg-RX-API-DMAC7.html)
EP (1) EP1884771B1 (cg-RX-API-DMAC7.html)
JP (1) JP5021183B2 (cg-RX-API-DMAC7.html)
CN (1) CN101203748B (cg-RX-API-DMAC7.html)
WO (1) WO2006123730A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103344639A (zh) * 2013-07-11 2013-10-09 山东大学 一种快速检测尿中吡咯加合物的检测管

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060091006A1 (en) * 1999-11-04 2006-05-04 Yi Wang Analyte sensor with insertion monitor, and methods
US7497940B2 (en) * 2003-09-02 2009-03-03 Koji Sode Glucose sensor and glucose level measuring apparatus
JP2008243380A (ja) * 2007-03-23 2008-10-09 Sony Corp 酵素固定化電極、燃料電池、電子機器、酵素反応利用装置および酵素固定化基体
US8268604B2 (en) * 2007-12-20 2012-09-18 Abbott Point Of Care Inc. Compositions for forming immobilized biological layers for sensing
AU2010286191B2 (en) 2008-09-17 2014-11-13 Avery Dennison Corporation Activatable adhesive, labels, and related methods
US9653006B2 (en) 2008-09-17 2017-05-16 Avery Dennison Corporation Activatable adhesive, labels, and related methods
CN102308204B (zh) * 2009-02-09 2014-07-09 爱科来株式会社 电化学传感器及其制备方法
JP5432575B2 (ja) * 2009-04-21 2014-03-05 グンゼ株式会社 バイオセンサ及びその製造方法
CN102414319A (zh) * 2009-04-30 2012-04-11 池田食研株式会社 蛋白质性电子介质
JP5665070B2 (ja) * 2009-09-25 2015-02-04 独立行政法人産業技術総合研究所 酸化還元タンパク質固定化ナノ構造電極
JP6435264B2 (ja) * 2013-08-07 2018-12-05 アークレイ株式会社 電気化学式バイオセンサを用いた物質の測定方法及び測定装置
WO2016199741A1 (ja) * 2015-06-08 2016-12-15 国立研究開発法人科学技術振興機構 高密度微小チャンバーアレイおよびこれを用いた測定方法
CN105355530B (zh) * 2015-10-30 2017-08-11 三诺生物传感股份有限公司 一种用于试条电极预处理的等离子处理装置和方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1321245A (zh) * 1999-09-13 2001-11-07 松下电器产业株式会社 脂质修饰酶的制备方法及生物传感器
US6773563B2 (en) * 2000-08-09 2004-08-10 Nec Corporation Electrochemical sensor having a reference electrode

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0810208A (ja) 1994-06-30 1996-01-16 Toshiba Corp 食器洗浄機
US5650062A (en) * 1995-03-17 1997-07-22 Matsushita Electric Industrial Co., Ltd. Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same
US6458599B1 (en) 2000-02-18 2002-10-01 Aspira Biosystems, Inc. Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules
AU2001241531A1 (en) * 2000-02-18 2001-08-27 Aspira Biosystems, Inc. Compositions and methods for surface imprinting
WO2002009647A2 (en) * 2000-07-28 2002-02-07 Emory University Biological component comprising artificial membrane
EP1331272B1 (en) * 2000-10-31 2007-06-06 Koji Sode Novel glucose dehydrogenase and process for producing the dehydrogenase
DK2330407T3 (da) * 2001-09-14 2013-06-24 Arkray Inc Fremgangsmåde, værktøj og indretning til at måle en koncentration
KR100729307B1 (ko) * 2002-06-17 2007-06-15 아크레이 인코퍼레이티드 글루코스 탈수소효소를 이용한 글루코스 농도 측정방법 및글루코스 센서
JP4637579B2 (ja) * 2002-07-25 2011-02-23 パナソニック株式会社 電解質膜とこれを用いた膜電極接合体および燃料電池
JP4250481B2 (ja) * 2003-08-11 2009-04-08 キヤノン株式会社 多孔質構造体
US7497940B2 (en) * 2003-09-02 2009-03-03 Koji Sode Glucose sensor and glucose level measuring apparatus
WO2005030807A1 (ja) * 2003-09-30 2005-04-07 Koji Sode グルコース脱水素酵素とシトクロームとの融合蛋白質

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1321245A (zh) * 1999-09-13 2001-11-07 松下电器产业株式会社 脂质修饰酶的制备方法及生物传感器
US6773563B2 (en) * 2000-08-09 2004-08-10 Nec Corporation Electrochemical sensor having a reference electrode

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Z. Salamon and G.Tollin.Interfacial electrochemistry of cytochrome c at a lipid bilayer modified electrode:effect of incorporation of negative charges into the bilayer on cyclic voltammetric parameters.《Bioelectrochemistry and Bioenergetics》.1991,第26卷(第2期),321-334. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103344639A (zh) * 2013-07-11 2013-10-09 山东大学 一种快速检测尿中吡咯加合物的检测管
CN103344639B (zh) * 2013-07-11 2015-10-28 山东大学 一种快速检测尿中吡咯加合物的检测管

Also Published As

Publication number Publication date
EP1884771B1 (en) 2017-12-13
EP1884771A1 (en) 2008-02-06
US9702843B2 (en) 2017-07-11
EP1884771A4 (en) 2011-10-19
US20150377818A1 (en) 2015-12-31
WO2006123730A1 (ja) 2006-11-23
CN101203748A (zh) 2008-06-18
JP5021183B2 (ja) 2012-09-05
US20090101499A1 (en) 2009-04-23
JP2006322889A (ja) 2006-11-30

Similar Documents

Publication Publication Date Title
US9702843B2 (en) Biosensor incorporating protein-immobilized membrane and method of immobilizing protein in biosensor
Forzani et al. Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine
Jung et al. Polymeric mercaptosilane-modified platinum electrodes for elimination of interferants in glucose biosensors
JP5052525B2 (ja) 埋め込み可能な被分析物質センサで使用するための膜
US6627057B1 (en) Microsphere containing sensor
US7086277B2 (en) Device having a flow channel containing a layer of wicking material
Alfonta et al. Sensing of acetylcholine by a tricomponent-enzyme layered electrode using faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance transduction methods
JP4073969B2 (ja) 小さなサンプル容量中の被検体の測定のための電極
CN101971012B (zh) 用于传感的固定化生物层的形成
US6551496B1 (en) Microstructured bilateral sensor
CA2474912C (en) Electrochemical biosensor strip for analysis of liquid samples
Kharitonov et al. The use of impedance spectroscopy for the characterization of protein-modified ISFET devices: application of the method for the analysis of biorecognition processes
Mostafa Electrochemical biosensors for the detection of pesticides
CN101191783B (zh) 生物传感器
CN1463362A (zh) 生物传感器
JP3910148B2 (ja) 排気口付き分析用具
CN102308204B (zh) 电化学传感器及其制备方法
JP2006322889A5 (cg-RX-API-DMAC7.html)
CN1789995A (zh) 用于检测被分析物的测量装置和测量方法
Turcu et al. Ink‐Jet Microdispensing for the Formation of Gradients of Immobilised Enzyme Activity
JP2009500601A (ja) 電極プリコンディショニング
Kumar et al. Biocompatible self-assembled monolayer platform based on (3-glycidoxypropyl) trimethoxysilane for total cholesterol estimation
Zhu et al. An overview of Si-based biosensors
JP2004212393A (ja) バイオセンサ
JP4000322B2 (ja) 酵素に基づくセンサーに利用するためのキャスタブル拡散膜

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20110914

CF01 Termination of patent right due to non-payment of annual fee