JP4848358B2 - 非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法 - Google Patents

非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法 Download PDF

Info

Publication number
JP4848358B2
JP4848358B2 JP2007501210A JP2007501210A JP4848358B2 JP 4848358 B2 JP4848358 B2 JP 4848358B2 JP 2007501210 A JP2007501210 A JP 2007501210A JP 2007501210 A JP2007501210 A JP 2007501210A JP 4848358 B2 JP4848358 B2 JP 4848358B2
Authority
JP
Japan
Prior art keywords
dna
protein
prokaryotic
unmethylated
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2007501210A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007525982A5 (enExample
JP2007525982A (ja
Inventor
シュミット、カール−ヘルマン
シュトラウベ、エーバーハルト
ルースブルム、シュテファン
デイグナー、ハンス−ペーター
ザックセ、スベア
レーマン、マルク
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SIRS Lab GmbH
Original Assignee
SIRS Lab GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE200410010928 external-priority patent/DE102004010928B4/de
Priority claimed from DE200510001889 external-priority patent/DE102005001889B4/de
Application filed by SIRS Lab GmbH filed Critical SIRS Lab GmbH
Publication of JP2007525982A publication Critical patent/JP2007525982A/ja
Publication of JP2007525982A5 publication Critical patent/JP2007525982A5/ja
Application granted granted Critical
Publication of JP4848358B2 publication Critical patent/JP4848358B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2007501210A 2004-03-05 2005-03-02 非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法 Expired - Fee Related JP4848358B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102004010928.1 2004-03-05
DE200410010928 DE102004010928B4 (de) 2004-03-05 2004-03-05 Protein zur Bindung prokaryonter DNA sowie Verfahren zur Trennung und Anreicherung prokaryonter DNA
DE200510001889 DE102005001889B4 (de) 2005-01-14 2005-01-14 Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet
DE102005001889.0 2005-01-14
PCT/EP2005/002198 WO2005085440A1 (de) 2004-03-05 2005-03-02 VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYONTER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CpG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET

Publications (3)

Publication Number Publication Date
JP2007525982A JP2007525982A (ja) 2007-09-13
JP2007525982A5 JP2007525982A5 (enExample) 2009-05-21
JP4848358B2 true JP4848358B2 (ja) 2011-12-28

Family

ID=34921210

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007501210A Expired - Fee Related JP4848358B2 (ja) 2004-03-05 2005-03-02 非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法

Country Status (8)

Country Link
US (1) US8481262B2 (enExample)
EP (1) EP1723233B1 (enExample)
JP (1) JP4848358B2 (enExample)
AT (1) ATE554167T1 (enExample)
CA (1) CA2558515C (enExample)
DK (1) DK1723233T3 (enExample)
ES (1) ES2384571T3 (enExample)
WO (1) WO2005085440A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE50207801D1 (de) 2002-09-18 2006-09-21 Sirs Lab Gmbh Verfahren zum Nachweis prokaryontischer DNA
DE202005009490U1 (de) * 2005-06-16 2006-10-19 Sirs-Lab Gmbh Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA
JP5246726B2 (ja) 2006-10-05 2013-07-24 株式会社ジャパンディスプレイウェスト シフトレジスタ回路および表示装置
DE102007041864B4 (de) * 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
US9063130B2 (en) 2007-09-11 2015-06-23 Kaneka Corporation Nucleic acid detection method and nucleic acid detection kit
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
AT509622A1 (de) 2010-04-08 2011-10-15 Greiner Bio One Gmbh Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren
DE102012219142B4 (de) * 2012-10-19 2016-02-25 Analytik Jena Ag Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies
DE102012021146A1 (de) 2012-10-25 2014-05-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Methode zur spezifischen Isolation von Nukleinsäuren
WO2016034405A1 (en) 2014-09-03 2016-03-10 Siemens Aktiengesellschaft A process for separation of prokaryotic dna from eukaryotic dna in a whole blood sample
WO2020216966A1 (en) * 2019-04-26 2020-10-29 Thermo Fisher Scientific Baltics Uab Isolated nucleic acid binding domains

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002034565A (ja) * 2000-07-19 2002-02-05 Japan Science & Technology Corp 細菌dnaを特異的に認識する受容体タンパク質
WO2004003010A2 (en) * 2002-07-01 2004-01-08 Inpharmatica Limited Nuclear hormone receptor ligano-binding domain containing protein
JP2005538737A (ja) * 2002-09-18 2005-12-22 ジルス−ラプ ゲーエムベーハー 原核生物dnaを濃縮する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239116B1 (en) 1994-07-15 2001-05-29 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
CA2224492A1 (en) * 1995-07-28 1997-02-13 Michael E. T. I. Boerrigter A method of and test kit for mutagenesis testing
AU7680300A (en) * 1999-08-18 2001-03-13 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Human dna sequences
SE0201257D0 (sv) * 2002-04-25 2002-04-25 Medical Invest In Sweden Ab Improved Separation
GB0423991D0 (en) * 2004-10-29 2004-12-01 Univ Edinburgh Applications of nucleic acid fragments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002034565A (ja) * 2000-07-19 2002-02-05 Japan Science & Technology Corp 細菌dnaを特異的に認識する受容体タンパク質
WO2004003010A2 (en) * 2002-07-01 2004-01-08 Inpharmatica Limited Nuclear hormone receptor ligano-binding domain containing protein
JP2005538737A (ja) * 2002-09-18 2005-12-22 ジルス−ラプ ゲーエムベーハー 原核生物dnaを濃縮する方法

Also Published As

Publication number Publication date
US20080003568A1 (en) 2008-01-03
US8481262B2 (en) 2013-07-09
ES2384571T3 (es) 2012-07-09
WO2005085440A8 (de) 2005-12-15
WO2005085440A1 (de) 2005-09-15
ATE554167T1 (de) 2012-05-15
CA2558515A1 (en) 2005-09-15
EP1723233B1 (de) 2012-04-18
CA2558515C (en) 2013-12-03
JP2007525982A (ja) 2007-09-13
EP1723233A1 (de) 2006-11-22
DK1723233T3 (da) 2012-07-23

Similar Documents

Publication Publication Date Title
JP4175670B2 (ja) 固体相核酸の単離
US8288115B2 (en) Method for enriching a prokaryotic DNA
EP2505666B1 (en) Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
JP4848358B2 (ja) 非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法
US20140038241A1 (en) Genomic enrichment method, composition, and reagent kit
CN111448315B (zh) 一种Cas蛋白体系分离DNA的方法
JP2007525982A5 (enExample)
EP0741141A2 (en) Method of purifying ologonucleotide from biological samples
DE102004010928B4 (de) Protein zur Bindung prokaryonter DNA sowie Verfahren zur Trennung und Anreicherung prokaryonter DNA
JPWO2009066502A1 (ja) 固体支持体
JP4163307B2 (ja) ベロトキシンの精製方法
CN121108268A (zh) 蛋白分子及其在鉴定多种血清型的禽致病性大肠杆菌中的应用
CN116804196A (zh) 一种用于检测hcmv病毒的核酸适配体、试剂盒及应用
CN115927244A (zh) 一种基于微流控技术的单细胞ChIP-seq文库的构建方法
JP2006042743A (ja) 細菌の回収方法
JP2006246732A (ja) 核酸精製用支持体および精製方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070822

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20071226

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090403

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101102

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110131

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110607

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110826

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110927

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20111017

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20141021

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20141021

Year of fee payment: 3

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20141021

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees