CA2558515C - Method of enriching and/or separating prokaryotic dna by means of a protein which specifically binds dna containing non-methylated cpg motifs - Google Patents
Method of enriching and/or separating prokaryotic dna by means of a protein which specifically binds dna containing non-methylated cpg motifs Download PDFInfo
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- CA2558515C CA2558515C CA2558515A CA2558515A CA2558515C CA 2558515 C CA2558515 C CA 2558515C CA 2558515 A CA2558515 A CA 2558515A CA 2558515 A CA2558515 A CA 2558515A CA 2558515 C CA2558515 C CA 2558515C
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- dna
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102004010928.1 | 2004-03-05 | ||
| DE200410010928 DE102004010928B4 (de) | 2004-03-05 | 2004-03-05 | Protein zur Bindung prokaryonter DNA sowie Verfahren zur Trennung und Anreicherung prokaryonter DNA |
| DE200510001889 DE102005001889B4 (de) | 2005-01-14 | 2005-01-14 | Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet |
| DE102005001889.0 | 2005-01-14 | ||
| PCT/EP2005/002198 WO2005085440A1 (de) | 2004-03-05 | 2005-03-02 | VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYONTER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CpG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2558515A1 CA2558515A1 (en) | 2005-09-15 |
| CA2558515C true CA2558515C (en) | 2013-12-03 |
Family
ID=34921210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2558515A Expired - Fee Related CA2558515C (en) | 2004-03-05 | 2005-03-02 | Method of enriching and/or separating prokaryotic dna by means of a protein which specifically binds dna containing non-methylated cpg motifs |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8481262B2 (enExample) |
| EP (1) | EP1723233B1 (enExample) |
| JP (1) | JP4848358B2 (enExample) |
| AT (1) | ATE554167T1 (enExample) |
| CA (1) | CA2558515C (enExample) |
| DK (1) | DK1723233T3 (enExample) |
| ES (1) | ES2384571T3 (enExample) |
| WO (1) | WO2005085440A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE50207801D1 (de) | 2002-09-18 | 2006-09-21 | Sirs Lab Gmbh | Verfahren zum Nachweis prokaryontischer DNA |
| DE202005009490U1 (de) * | 2005-06-16 | 2006-10-19 | Sirs-Lab Gmbh | Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA |
| JP5246726B2 (ja) | 2006-10-05 | 2013-07-24 | 株式会社ジャパンディスプレイウェスト | シフトレジスタ回路および表示装置 |
| DE102007041864B4 (de) * | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| US9063130B2 (en) | 2007-09-11 | 2015-06-23 | Kaneka Corporation | Nucleic acid detection method and nucleic acid detection kit |
| US20090137024A1 (en) * | 2007-11-28 | 2009-05-28 | Canon U.S. Life Sciences, Inc. | Method of Separating Target DNA from Mixed DNA |
| AT509622A1 (de) | 2010-04-08 | 2011-10-15 | Greiner Bio One Gmbh | Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren |
| DE102012219142B4 (de) * | 2012-10-19 | 2016-02-25 | Analytik Jena Ag | Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies |
| DE102012021146A1 (de) | 2012-10-25 | 2014-05-15 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Methode zur spezifischen Isolation von Nukleinsäuren |
| WO2016034405A1 (en) | 2014-09-03 | 2016-03-10 | Siemens Aktiengesellschaft | A process for separation of prokaryotic dna from eukaryotic dna in a whole blood sample |
| WO2020216966A1 (en) * | 2019-04-26 | 2020-10-29 | Thermo Fisher Scientific Baltics Uab | Isolated nucleic acid binding domains |
Family Cites Families (8)
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|---|---|---|---|---|
| US6239116B1 (en) | 1994-07-15 | 2001-05-29 | University Of Iowa Research Foundation | Immunostimulatory nucleic acid molecules |
| CA2224492A1 (en) * | 1995-07-28 | 1997-02-13 | Michael E. T. I. Boerrigter | A method of and test kit for mutagenesis testing |
| AU7680300A (en) * | 1999-08-18 | 2001-03-13 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Human dna sequences |
| JP2002034565A (ja) | 2000-07-19 | 2002-02-05 | Japan Science & Technology Corp | 細菌ｄｎａを特異的に認識する受容体タンパク質 |
| SE0201257D0 (sv) * | 2002-04-25 | 2002-04-25 | Medical Invest In Sweden Ab | Improved Separation |
| GB0215228D0 (en) * | 2002-07-01 | 2002-08-14 | Inpharmatica Ltd | Protein |
| DE50207801D1 (de) * | 2002-09-18 | 2006-09-21 | Sirs Lab Gmbh | Verfahren zum Nachweis prokaryontischer DNA |
| GB0423991D0 (en) * | 2004-10-29 | 2004-12-01 | Univ Edinburgh | Applications of nucleic acid fragments |
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2005
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- 2005-03-02 ES ES05715667T patent/ES2384571T3/es not_active Expired - Lifetime
- 2005-03-02 JP JP2007501210A patent/JP4848358B2/ja not_active Expired - Fee Related
- 2005-03-02 WO PCT/EP2005/002198 patent/WO2005085440A1/de not_active Ceased
- 2005-03-02 DK DK05715667.1T patent/DK1723233T3/da active
- 2005-03-02 US US10/591,633 patent/US8481262B2/en not_active Expired - Fee Related
- 2005-03-02 AT AT05715667T patent/ATE554167T1/de active
- 2005-03-02 EP EP05715667A patent/EP1723233B1/de not_active Expired - Lifetime
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| US20080003568A1 (en) | 2008-01-03 |
| US8481262B2 (en) | 2013-07-09 |
| ES2384571T3 (es) | 2012-07-09 |
| WO2005085440A8 (de) | 2005-12-15 |
| WO2005085440A1 (de) | 2005-09-15 |
| JP4848358B2 (ja) | 2011-12-28 |
| ATE554167T1 (de) | 2012-05-15 |
| CA2558515A1 (en) | 2005-09-15 |
| EP1723233B1 (de) | 2012-04-18 |
| JP2007525982A (ja) | 2007-09-13 |
| EP1723233A1 (de) | 2006-11-22 |
| DK1723233T3 (da) | 2012-07-23 |
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