JP4791750B2 - Dnaなどの分離精製方法及び分離精製機構 - Google Patents
Dnaなどの分離精製方法及び分離精製機構 Download PDFInfo
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- JP4791750B2 JP4791750B2 JP2005118750A JP2005118750A JP4791750B2 JP 4791750 B2 JP4791750 B2 JP 4791750B2 JP 2005118750 A JP2005118750 A JP 2005118750A JP 2005118750 A JP2005118750 A JP 2005118750A JP 4791750 B2 JP4791750 B2 JP 4791750B2
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- monolith
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Description
モノリス構造体を融着した物では、6本とも抜ける事が無かった。
2 ピペット
3 チューブ
4 ウェルプレート
5 カートリッジ
Claims (6)
- 粘性の生体試料液中のDNAを、容器に取付けたシラノール基を持ち、スルーポア及びメソポアを有する3次元網目構造のモノリス構造体であって、硬度30Hk以上120Hk以下の耐圧性を有する分離体を通過させる過程により、捕捉し溶出、精製させるDNAの分離精製方法。
- 前記生体試料液が血液、動物組織あるいは大腸菌培養液であることを特徴とする請求項1記載のDNAの分離精製方法。
- 焼成することにより硬化させた前記モノリス構造体の分離体を用いることを特徴とする請求項1又は2記載のDNAの分離精製方法。
- ディスポーザル容器等の通液部に、シラノール基を有し、硬度30Hk以上120Hk以下のスルーポア及びメソポアを有する3次元網目構造のモノリス構造体の分離体を生成形成あるいは融着固定させたことを特徴とするDNAの分離精製機構。
- ウェルプレートの各穴にシラノール基を有し、硬度30Hk以上120Hk以下のスルーポア及びメソポアを有する3次元網目構造のモノリス構造体の分離体を夫々設けたことを特徴とするDNAの分離精製機構。
- 試料の粘度に応じ、前記モノリス構造のスルーポア及び/またはメソポアのポアサイズを制御可能とすることを特徴とする請求項4又は5記載のDNAの分離精製機構。
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JP4791750B2 true JP4791750B2 (ja) | 2011-10-12 |
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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US7759112B2 (en) * | 2007-10-31 | 2010-07-20 | Akonni Biosystems, Inc. | Apparatus, system, and method for purifying nucleic acids |
KR20110028295A (ko) | 2008-06-12 | 2011-03-17 | 스미토모 베이클리트 컴퍼니 리미티드 | 당쇄 시료 조제 방법, 당쇄 시료, 및 당쇄 분석법 |
JP2010200661A (ja) * | 2009-03-03 | 2010-09-16 | Toyobo Co Ltd | 簡便かつ迅速な核酸抽出方法 |
WO2011158815A1 (ja) * | 2010-06-18 | 2011-12-22 | 東洋紡績株式会社 | 抗酸菌の溶菌とその核酸の分離を同時に行う方法 |
JP2013063064A (ja) * | 2011-08-29 | 2013-04-11 | Sumitomo Bakelite Co Ltd | 処理具 |
JP5974778B2 (ja) * | 2012-01-20 | 2016-08-23 | 住友ベークライト株式会社 | 処理具 |
JP5880626B2 (ja) * | 2014-06-17 | 2016-03-09 | 東洋紡株式会社 | 簡便かつ迅速な核酸抽出方法 |
WO2017119503A1 (ja) * | 2016-01-08 | 2017-07-13 | 株式会社ジーンデザイン | ゾル-ゲル法で作製される無機多孔質体を用いた核酸合成用担体 |
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DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
EP0826412A3 (en) * | 1996-08-26 | 1999-06-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin | Method for producing filter elements and the filter elements thus produced |
JP3985170B2 (ja) * | 1996-12-26 | 2007-10-03 | 直弘 曽我 | 無機系多孔質体の製造方法 |
US6503397B2 (en) * | 1997-12-05 | 2003-01-07 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
JP2002000255A (ja) * | 2000-06-22 | 2002-01-08 | Hitachi Ltd | 核酸抽出装置 |
JP2002191351A (ja) * | 2001-10-19 | 2002-07-09 | Hitachi Ltd | 核酸の精製用装置および核酸捕捉用チップ |
WO2004033470A2 (en) * | 2002-10-04 | 2004-04-22 | Whatman, Inc. | Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems |
JP4222853B2 (ja) * | 2003-02-18 | 2009-02-12 | Agcエスアイテック株式会社 | 耐アルカリ性化学修飾型シリカゲルの製造方法 |
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