JP4772354B2 - Life extension agent made from rice - Google Patents

Description

The present invention relates to a life extension agent (immortalizing agent) for life forms and cells, which contains a rice-derived component as an active ingredient.

Regarding the immortalization of living organisms and cells, the existence of telomeres is known. A telomere is a special part that exists at both ends of DNA in a cell, and functions so as to be shortened at each cell division and carve out aging. A special enzyme that maintains the length of telomeres is called an enzyme called telomerase. Cancer cells maintain their telomere length, acquire permanent division ability, and achieve immortalization. Yes. Similarly, it is also known that vitamin C suppresses the shortening of DNA related to telomeres.

However, the immortalization described above is to keep the DNA before cell division and the DNA after cell division substantially in the same state by preventing the telomere from being removed even after repeated cell division. Yes, lifespans and cells themselves do not extend their lives.
Japanese National Patent Publication No. 08-501079

Therefore, the present invention aims to find a novel component effective for immortalization (life extension) in the sense that the lifespan of living organisms and cells themselves is extended, not immortalization in the sense of acquiring infinite replication ability. And

The inventors of the present invention have been researching various plant components, mainly rice, which is a staple food, from the viewpoint of combining plants and animals. In the process, it has been found that rice has many possibilities and benefits that could not have been predicted before. Therefore, we have taken up the theme of rice, which is used as a staple food and has proven to be the safest, and has conducted comprehensive rice research. As one of the themes, we have conducted intensive research on life extension. In the process, we found that rice contains ingredients that have a life extension effect, and completed the present invention. .

In the present invention, it has not yet been elucidated as to what kind of component derived from rice (or a combination of a plurality of components or a combination of a plurality of components at a predetermined abundance ratio) has a life extension effect. It has been found that those subjected to a predetermined treatment using as a raw material show a life extension effect. In addition, the mechanism of life extension is not clear, but in situations where it is difficult to survive, the cell cycle is stopped or the cell activity is lowered (it may be adjusted at the gene level), and then the environment is rested. The so-called “hibernation theory” is to revive these if the situation improves
Seems to be involved. Moreover, the life extension agent according to the present invention further comprises cell activity,
It has also been found that it has a function of regulating the cell cycle and gene function normally, and a better state.

  That is, the present invention (1) is a life extending agent containing rice pulverized material as an active ingredient.

  The present invention (2) is a life extending agent containing rice extract as an active ingredient.

This invention (3) is a life extension agent which contains as an active ingredient what hydrolyzed rice, or the thing which made the rice cake act.

The present invention (4) is a life extension agent containing, as an active ingredient, a product which has been subjected to enzymatic degradation or koji before, during or after extraction of rice.

The present invention (5) is a life extension agent containing, as an active ingredient, a product obtained by subjecting an extract of rice or enzymatic degradation or koji to alcohol fermentation or organic acid fermentation.

The present invention (6) is the life extension agent according to any one of the above (1) to (5), which is a life extension agent for a living organism or an individual, an organ, an organ, a tissue, a body fluid, or a cell.

The present invention (7) is any one of the above (1) to (5) for regulating cell function, cell activity, cell cycle, gene function normally, maintaining youthfulness and preventing aging. Is one life-extending agent.

The present invention (8) is for improving or improving the function of a living organism or an individual, an organ, an organ, a tissue, a body fluid, or a cell, normalization, restoration, prevention of aging, maintaining youthfulness, or rejuvenation. 1
) To (5).

The present invention (9) relates to the circulatory system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue /
Organ, spleen, thymus), skin, skin appendages, digestive system (lips, tonsils, teeth, esophagus, stomach, small intestine, large intestine, liver, bile duct, spleen), respiratory system (nose, pharynx, larynx, trachea) , Lung), urinary system (kidney, urinary tract), sexual reproduction system (male genital organ, female genital organ), endocrine system (pituitary gland, pineal gland, thyroid gland)
Adrenal gland), brain, nervous system (peripheral nerve, central nervous system), metabolic system (sugar, lipid, uric acid metabolism), balance / hearing device, visual organ, mucosal system, motor organ (bone, joint, muscle, tendon, ligament) (1) to (5) for improvement or improvement of functions such as), normalization, restoration, prevention of aging, maintenance of youthfulness or rejuvenation
) One of the life-extending agents.

The present invention (10) is any one of the above (1) to (5) for preventing, preventing, improving and treating diseases and various symptoms in living organisms or individuals, organs, organs, tissues, body fluids or cells. Is one life extension agent.

The present invention (11) includes the circulatory system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue / organ, spleen, thymus), skin, skin appendage, digestive system (lip, tonsil, Teeth, esophagus, stomach, small intestine, large intestine, liver, bile duct system, spleen), respiratory system (nose, pharynx, larynx, trachea, lung), urinary system (
Kidney, urinary tract), sex breeding system (male genital organ, female genital organ), endocrine system (pituitary gland, pineal gland, thyroid gland, adrenal gland), brain, nervous system (peripheral nerve, central nervous system), metabolic system (sugar, lipid) Uric acid metabolism), equilibrium
The above (1) to (5) for prevention, prevention, improvement and treatment of diseases and symptoms in auditory organs, visual organs, mucosal systems, motor organs (bones, joints, muscles, tendons, ligaments), etc. Is one of the life-extending agents.

The present invention (12) relates to skin disorders caused by epidermis / dermis, cell disorders, DNA disorders, atopic dermatitis, dry skin and other dry skin symptoms (atopy skin, dry / sozoform skin, aging skin, ichthyosis) , Bulky skin, rough skin, sebum deficiency, xeroderma, dry eczema, progressive palmar keratosis, erythema, sclerosis / keratinization, cracks, scales, crest, itching, scarring), spots, buckwheat, pruritus, Skin rash, rough skin, tingling, cracks, redheads, skin aging symptoms (wrinkles, reduced elasticity, sagging, spots), oily skin, acne, dyschromia, UV-induced skin damage (spots, freckles), photoinflammation, dullness , Darkening, physical / chemical skin disorders (cuts, burns, bedsores), water,
Skin symptoms caused by the use of soaps, detergents, surfactants, solvents, side effects of pharmaceuticals such as topical skin preparations, biological skin disorders (skin infections), athlete's foot, dermatitis, eczema, wound healing, hair growth, hair restoration The life-extending agent according to any one of (1) to (5) above, for preventing, preventing, improving, and treating hair growth and alopecia.

The present invention (13) is the life extension agent according to any one of the above (1) to (5) for preventing, preventing, improving and treating spots, buckwheat and senile pigment spots.

In the present invention (14), various inflammations, infections, allergies, immune dysfunctions, mucosal disorders, cell disorders, DNA disorders, tumors, ulcers, hyperlipidemia, obesity, diabetes, arteriosclerosis, gout, blood pressure restoration , Improvement (high blood pressure, low blood pressure), blur, dementia, rhinitis, asthma, eye symptoms, cold, vascular disorder,
Uchimi, stiff shoulders, lottery, neuralgia, low back pain, rheumatism, hemorrhoids, recovery from fatigue, coldness before and after childbirth,
Stomatitis, throat inflammation, pain, sore throat, laryngitis, tonsillitis, periodontitis, alveolar pyorrhea, gingivitis, osteoporosis, nourishing tonic, frail constitution, physical fatigue, low physical fitness, salt damage prevention, Helicobacter pylori growth inhibition, For the prevention of stress, side effects caused by pharmaceuticals, prevention of deterioration, improvement, treatment, (1
) To (5).

The present invention (15) is any one of the above (1) to (5), wherein the tumor prevention, deterioration prevention, improvement and treatment are cancer prevention, deterioration prevention, progression inhibition, metastasis prevention, improvement and treatment. Life extension agent.

The present invention (16) is the life extension agent according to any one of the above (1) to (5) for improving, improving, normalizing, improving or preventing aging, maintaining youthfulness or rejuvenating sexual function. is there.

The present invention (17) relates to the preservation of living organisms or individuals, organs, organs, tissues, body fluids or cells,
The life extension agent according to any one of the above (1) to (5) for maintaining freshness.

According to the present invention, the life span of living organisms and cells is extended. Therefore, for example, it is suitable for use of organ preservation solution, cell preservation solution, etc. in organ transplantation and the like. Moreover, if it is applied to blood that currently has a limited storage period, the usable period can be extended. Furthermore, the youthfulness of cells is maintained, cell activation is improved, cells rejuvenate, and life forms such as cancer prevention / treatment, visceral function improvement / improvement, spot prevention / treatment, skin health or hair growth In addition, an effect based on extending the life of cells can be expected.

In addition, rice has been a staple food so far, and new uses have hardly been developed. Furthermore, rice has been regarded as a staple food so far, and its safety has been proven. In other words, the present invention has been found not only to have a very excellent life extension effect, but also to have a sufficiently demonstrated safety, various pharmaceuticals, quasi drugs, cosmetics, foods It can be said that it is a very significant invention that can be used for the above. In addition, it is extremely meaningful to find new uses for rice, and to increase consumption by improving the image of rice, which is now said to be overproduction of rice.

The “rice” according to the present invention is a concept including not only rice such as white rice, brown rice and germinated rice, but also part of rice such as white rice and red rice bran. However, the rice must contain a white rice portion, that is,
It is preferable to use white rice, brown rice, germinated rice, and white birch. Here, regarding white rice and brown rice, it refers to brown rice and white rice such as sticky rice and brown rice, regardless of japonica and indica rice, and any kind and kind. In addition, white shark and red shark generally appear at the time of whitening.
Although it indicates 2% or more of red cocoon and 92% or less of white cocoon, a mixture of both may be used. In addition, since the active ingredient is stable to heat and light, the above-mentioned raw materials are dipping, steaming, roasting (pointing to all of sand roasting, net roasting, hot air roasting, etc.), steaming roasting, freeze drying It may be subjected to raw material treatment such as surface modification such as UV irradiation, photo-modification such as UV irradiation, pressure roasting such as Patrice, frying. White rice, brown rice, and germinated rice are effective when used as they are, but are preferably pulverized for practical use. In order to pulverize white rice, brown rice and germinated rice into powder, a general method may be used using a pulverizer or a rice mill.

In the case of producing germinated rice, germinated rice is immersed in water or sprayed with water. The temperature at the time of germination is 5-70 degreeC. However, the temperature and time are not limited as long as germination occurs. In addition, when there is a risk of water rot during germination, it is preferable to replace the water so that it does not rot or to perform some preservative. Here, germination refers to everything from just before germination to germination. The germinated rice is washed thoroughly before use. At this time, you may dry and use. When rice is extracted, or enzymatically decomposed or subjected to koji, the raw material rice is pulverized into granules or powders, so that the surface area is increased and the efficiency is improved. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the rice tissue.

When water is extracted from rice, a high extraction temperature is efficient, but extraction can be sufficiently performed even at a low temperature. However, in the case of a low temperature of 40 ° C. or lower, it is desirable that the pH is made acidic or alkaline, or a preservative or alcohol is added so that the rice is not spoiled. The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined by the extraction temperature. The extraction may be performed under pressure, normal pressure, or reduced pressure. In the case of water extraction, the most serious problem is the gelatinization phenomenon. If it becomes paste-like, not only extraction efficiency will worsen but it will be extremely difficult in actual work. In order to prevent this, the reaction may be performed by adding amylase or acidifying with hydrochloric acid or the like to cut the starch. By using this method, the problem can be solved sufficiently and there is no problem in practical use.

It is also effective to perform acid decomposition extraction or alkali decomposition extraction because the active ingredient in the extract is stable to acid and alkali. In this case, neutralization and desalting are performed as necessary. Also when extracting with an organic solvent, it is desirable to extract rice by pulverizing or pulverizing it as much as possible. The organic solvent may be a common organic solvent such as alcohol, acetone, n-hexane, methanol or the like, but those which are harmful to the human body are preferably safe because the solvent needs to be completely removed after extraction. In addition, rice may be enzymatically degraded, or rice bran may be allowed to act. Enzymatic degradation here refers to one or more enzymes that act on rice, such as starch degrading enzymes (liquefaction enzymes, saccharifying enzymes), proteolytic enzymes, lipolytic enzymes, fiber degrading enzymes, lignin degrading enzymes, pectin degrading enzymes. It means to act. For example, the combination of a liquefying enzyme and a saccharifying enzyme can be mentioned. In addition, the type of koji mold and the variety and type of rice are not questioned.

Furthermore, in performing the said extraction, you may make said enzyme decomposition | disassembly and soot act before extraction, simultaneous with extraction, or after extraction. In the present invention, when the above treatment is further performed or simultaneously after the treatment, alcohol fermentation or lactic acid fermentation, and organic acid fermentation such as acetic acid fermentation,
The following points are also effective. When alcoholic fermentation is performed, not only is there no stickiness at the time of application, but it is easy to concentrate and the active ingredient is easily concentrated. The sugar may be removed by aeration fermentation with yeast, alcohol precipitation, or the like.

When the life extension agent according to the present invention is used, the life of a living organism or the life of a cell is extended. Here, a living organism is a concept that includes not only single-celled organisms but also multicellular organisms (including higher animals such as mammals including humans). In addition, the cell is not limited to a single cell, but is a concept including organs, organs, tissues, body fluids, and the like that are composed of a plurality of cells.

The product of the present invention obtained as described above is used as it is, or after being squeezed and filtered without separating the residue. When it is used as it is, it is sterilized or sterilized to make a product. When the product of the present invention is used as an external preparation, cosmetics such as cream, face wash, emulsion, lotion, cleansing, pack, soap, etc., ointment, pasta, Use lotions, tinctures, ligaments, jellies, aerosols and other dosage forms. Moreover, it can also be used in dosage forms such as soft capsules, hard capsules, tablets, syrups and other oral preparations, and injections. Furthermore, there may be a prescription added to foods and beverages. In addition, you may add another compounding component and a chemical | medical agent according to a use.

Moreover, the lifetime extension agent which concerns on this invention is useful for various specific uses. For example, use of organ preservation solution or cell preservation solution in organ transplantation, etc .; use in blood; improvement or improvement, normalization, and restoration of functions of living organisms or individuals, organs, organs, tissues, body fluids or cells Anti-aging,
Youthfulness retention or rejuvenation; circulatory system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue / organ, spleen, thymus), skin, skin appendages, digestive system (lip, tonsils, Teeth, esophagus,
Stomach, small intestine, large intestine, liver, bile duct system, spleen), respiratory system (nose, pharynx, larynx, trachea, lung), urinary system (kidney, urinary tract), sex breeding system (male genital organ, female genital organ), endocrine System (pituitary gland, pineal gland,
Thyroid, adrenal gland), brain, nervous system (peripheral nerve, central nervous system), metabolic system (sugar, lipid, uric acid metabolism),
Improving or improving functions of balance / auditor, visual organ, mucosal system, motor organs (bone, joint, muscle, tendon, ligament), normalization, restoration, prevention of aging, preserving youthfulness or rejuvenation; life Prevention, improvement, treatment of diseases and symptoms in the body or individuals, organs, organs, tissues, body fluids or cells; cardiovascular system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue / organ, Spleen, thymus),
Skin, skin appendages, digestive system (lips, tonsils, teeth, esophagus, stomach, small intestine, large intestine, liver, bile duct, spleen), respiratory system (nose, pharynx, larynx, trachea, lungs), urinary system ( Kidney, urinary tract), sex breeding system (
Male and female genitalia), endocrine system (pituitary gland, pineal gland, thyroid, adrenal gland), brain, nervous system (
Peripheral nerve, central nerve), metabolic system (sugar, lipid, uric acid metabolism), balance / hearing device, visual organ, each mucosal system, motor organs (bone, joint, muscle, tendon, ligament), etc. Prevention, prevention of deterioration, improvement, treatment; skin disorders caused by epidermis / dermis, cell disorders, DNA disorders, atopic dermatitis, dry skin symptoms such as dry skin (atopy skin, dry / sozoized skin, aging skin, Ichthyosis, bulky skin, rough skin, sebum deficiency, xeroderma, dry eczema, progressive palmokeratosis,
Erythema, sclerosis / keratinization, cracks, scales, itchy, itching, scarring), spots, buckwheat, pruritus, skin rash, roughness, moistness, cracks, redheads, skin aging symptoms (wrinkles, reduced elasticity, sagging, sagging, Stains), oily skin, acne, pigmentation disorders, skin damage caused by ultraviolet rays (stains, buckwheat),
Photoinflammation, dullness, darkening, physical / chemical skin disorders (cuts, burns, bedsores), water, soaps, detergents, surfactants, skin effects caused by the use of solvents, side effects of pharmaceuticals such as external preparations for skin, biological Prevention, improvement, treatment of static skin disorders (skin infections), athlete's foot, dermatitis, eczema, wound healing, hair growth, hair restoration, hair growth, alopecia; prevention, improvement of spots, buckwheat, senile pigment spots,
In the case of treatment; various inflammations, infections, allergies, immune dysfunction, mucosal disorders, cell disorders,
DNA disorder, tumor, ulcer, hyperlipidemia, obesity, diabetes, arteriosclerosis, gout, blood pressure restoration, improvement (hypertension, hypotension) blur, dementia, rhinitis, asthma, eye symptoms, cold, blood vessels Disability, internal stiffness, stiff shoulders, lottery, neuralgia, low back pain, rheumatism, hemorrhoids, recovery from fatigue, prepartum cold disease, stomatitis, throat inflammation, pain, sore throat, laryngitis, tonsillitis, periodontitis, alveolar effusion, gingiva Flame, osteoporosis,
Nourishing tonic, frail constitution, physical fatigue, physical fitness decline, salt damage prevention, Helicobacter pylori growth inhibition,
Prevention, improvement, treatment of side effects caused by stress and pharmaceuticals; When tumor prevention, prevention of deterioration, improvement, treatment is cancer prevention, prevention of deterioration, progression suppression, prevention of metastasis, improvement, treatment; improvement of sexual function, improvement, Deterioration prevention, normalization, restoration or prevention of aging, preserving youthfulness or rejuvenation;
Examples include preservation of an individual, organ, organ, tissue, body fluid, cell, etc., and maintaining freshness. Note that the “freshness preservation” has not only an effect on humans, but also freshness preservation effects in fish (including raw fish), fresh flowers, and fresh foods such as meat, fish, and vegetables. Note that the above parentheses are given as examples and are not limited thereto.

Example 1
The white rice was put into a pulverizer to obtain 500 g of pulverized white rice. 10g of starch-degrading enzyme
And 1500 mL of water were added. Thereafter, the temperature was gradually raised, boiling and extracting for 5 minutes, and then cooled. Then, it squeezed with the squeezer and obtained the product of the present invention.

Example 2
White rice was pulverized to obtain 500 g of pulverized white rice. 300 g of rice bran and water 15
00 mL was added and left at 55 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain the product of the invention.

Example 3
White rice was pulverized to obtain 500 g of pulverized white rice. Proteolytic enzyme 2
g, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 mL of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised and the mixture was boiled and extracted for 5 minutes and then cooled. Yeast was added to this enzymatic degradation extract, and alcohol fermentation was performed for 7 days. Then, it squeezed with the squeezer and obtained the product of the present invention.

Example 4
White rice was pulverized to obtain 500 g of pulverized white rice. Proteolytic enzyme 2
g, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 mL of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised and the mixture was boiled and extracted for 5 minutes and then cooled. Lactic acid bacteria were added to this enzymatic degradation extract, and lactic acid fermentation was performed for 2 days. Then, it squeezed with the squeezer and obtained the product of the present invention.

Example 5
Lactic acid bacteria were added to 1500 mL of the rice extract obtained in Example 1, and lactic acid fermentation was performed for 2 days. Thereafter, lactic acid bacteria were filtered off with a filter to obtain a product of the present invention.

Example 6
Yeast was added to 1500 mL of the rice extract obtained in Example 2, and alcohol fermentation was performed for 7 days.
Thereafter, the yeast was separated by a filter to obtain the product of the present invention.

Example 7
1 kg of rice with germs attached was soaked in water at 30 ° C. for 3 days to absorb water and germinate the rice. The germinated rice was thoroughly washed, dried at 50 ° C. for 24 hours, and then finely pulverized to obtain 950 g of the product of the present invention.

Example 8
10 g of amylolytic enzyme and 1500 mL of water were added to 500 g of the ground product of the germinated rice obtained in Example 7. Thereafter, the temperature was gradually raised and the enzyme was allowed to act, followed by boiling extraction for 5 minutes and cooling. The extract was squeezed with a squeezer to obtain 1420 mL of the product of the present invention and 500 g of residue.

Example 9
Brown rice was pulverized well with a pulverizer to obtain 500 g of pulverized brown rice. Water 150
0 mL and 300 g of sputum were added, and the mixture was left at 55 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1500 mL of the product of the present invention and 700 g of residue.

Example 10
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. 2 g of proteolytic enzyme is added to this pulverized product,
2 g lipolytic enzyme, 2 g fiber degrading enzyme, 2 g starch degrading enzyme, 2 g pectin degrading enzyme and water 1
500 mL was added and left at 50 ° C. for 24 hours. Thereafter, yeast was added and alcohol fermentation was performed for 16 days. Then, it squeezed with the squeezer and obtained 1600 mL of this invention products and 300 g of residue.

Example 11
White rice was pulverized to obtain 500 g of pulverized white rice. Proteolytic enzyme 2
g, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 mL of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised and the mixture was boiled and extracted for 5 minutes and then cooled. The extract is squeezed with a squeezer, 1470 mL of the present product and 450 g of residue.
Got.

Example 12
White rice was pulverized to obtain 500 g of pulverized white rice. To this pulverized product, 10 g of amylolytic enzyme and 1500 mL of water were added. Thereafter, the temperature was gradually raised and the enzyme was allowed to act, followed by boiling extraction for 5 minutes and cooling. The extract was squeezed with a squeezer to obtain 1420 mL of the product of the present invention and 500 g of residue.

Example 13
White rice was pulverized to obtain 500 g of pulverized white rice. To this pulverized product, 10 g of amylolytic enzyme and 1500 mL of water were added. The temperature was then gradually raised, boiled and extracted for 5 minutes, and cooled. To this extract, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme and 2 g of pectin degrading enzyme were added and left at 50 ° C. for 24 hours. Thereafter, the product was squeezed with a squeezer to obtain 1500 mL of the present product and 400 g of a residue.

Example 14
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. 10g of starch-degrading enzyme
And 1500 mL of water were added. After that, gradually raise the temperature, let the enzyme act,
Boiled for min and cooled. To this, 100 mL of 95% ethanol was added, and acetic acid fermentation was performed for 20 days. Thereafter, filtration was performed to obtain 1400 mL of the present product.

Example 15
The proteolytic enzyme 2 is added to 490 g of white koji with a polishing rate of 50-70% and 10 g of red koji with a purity of 92% or more.
g, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 mL of water, and left at 50 ° C. for 20 hours. Then gradually increase the temperature.
After boiling and extracting for 30 minutes, it is cooled to 30 ° C. and squeezed with a squeezer, 1400 mL of the present product and residue 55
0 mL was obtained.

Example 16
To 300 g of the residue obtained in Example 10, add 500 mL of warm water at 60 ° C.
The temperature was adjusted to 0 ° C. and left for 5 hours while maintaining the temperature. Thereafter, the mixture was gradually heated, boiled and extracted for 5 minutes, and cooled. The extract was squeezed with a squeezer to obtain 480 mL of the product of the present invention.

Test example 1
Method: Intestinal mucosal epithelial cells (IEC-6) cultured confluently were cultured in a culture medium supplemented with a 1% sample for 30 days. The medium was not changed after adding 1% of each sample.
Sample: Example 1 to Example 6. In Examples 3 and 6, the sample was concentrated under reduced pressure using a rotary evaporator, the alcohol was distilled off, water was added, and the amount returned to the original amount was used as a sample.
Results: As a result of macroscopic observation of IEC-6 after adding 1% of each sample and culturing for 30 days,
No abnormalities were observed, and it was found that they remained normal and remained alive. The cells were young. On the other hand, the cells to which no sample was added could not continue to survive. From the above test, the life extension effect by the product of the present invention was proved. It also prevents aging,
A youthfulness retention effect was also observed.

Test example 2
Method: Gastric mucosal epithelial cells (RGM-1) cultured confluently were cultured in a culture solution containing 3% ethanol and 1% of each sample, and the survival activity of RGM-1 was evaluated by WST-1 assay. did. Moreover, the cytopathic inhibitory effect of the rice extract was evaluated by measuring LDH release in the culture solution.
Sample: Example 1
Results: As shown in FIGS. 1 and 2, by adding 1% of the sample, the RGM-1 survival activity was enhanced, the cell function was improved, improved, normalized, soundened, and cytotoxicity-preventing effects were observed. .

Test example 3
Method: A gastric mucosal epithelial cell (RGM-1) cultured confluently in a single layer was formed into a circular cell defect site with a diameter of 1 mm, and cultured in a culture medium supplemented with 3% ethanol and 1% sample. The damage repair effect was evaluated by measuring the area of the defect site over time.
Sample: Example 1
Results: As shown in Table 1, by adding 1% of the sample, cell damage was quickly repaired, and cell growth enhancement and damage repair promoting effects were observed.
From the above test, normalization, improvement, improvement, and healing effect of cell damage by the product of the present invention were demonstrated.

From Test Examples 1 to 3, the product of the present invention has a life extension, rejuvenation, retention of youthfulness, prevention of aging, improvement of function, improvement, normalization, restoration, prevention of failure, improvement, prevention of deterioration, therapeutic effect Was recognized. In addition, it has been revealed that it has the effect of regulating cell function, cell activity, cell cycle, and gene function normally, and in a better condition. It did not promote canceration.

According to Test Example 2, the extent to which the life extension agent according to the present invention affects the survival activity of gastric mucosal epithelial cells was examined. According to Test Example 2, the extent to which the life extension agent according to the present invention affects the LDH activity of gastric mucosal epithelial cells was examined.

Claims (4)

An agent for inhibiting cell damage caused by ethanol or a cell damage-repairing agent caused by ethanol, comprising, as an active ingredient, rice starch that has been subjected to amylolytic enzyme or koji before, during or after extraction. The inhibitor of cell damage caused by ethanol or the repair of cell damage caused by ethanol according to claim 1, which contains, as an active ingredient, a substance obtained by causing starch-degrading enzyme or rice bran to act on rice and further subjecting it to alcohol fermentation or organic acid fermentation. Agent . The agent for inhibiting cell damage by ethanol or the agent for repairing cell damage by ethanol according to claim 1 or 2, for improving the function of the digestive system or each mucosal system. The agent for inhibiting cell damage by ethanol or the agent for repairing cell damage by ethanol according to any one of claims 1 to 3, wherein the cells are small intestinal mucosal epithelial cells or gastric mucosal epithelial cells.

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