JP5296957B2 - Anti-aging agent for oral use - Google Patents

Description

  The present invention relates to an oral antiaging agent containing a rice-derived component as an active ingredient.

  No one can escape from the reality that aging appears as people age and eventually dies. However, if we can prevent aging, we can stay healthy until just before death. Aging means that the function of the body declines with age. That is, it is a phenomenon in which the function of each organ and the integration function between organs decrease with aging, and the homeostasis cannot be maintained. Several theories have been proposed for the cause of aging, for example, the telomere theory that cell division stops due to shortening of telomeres at both ends of the chromosome and causes aging, and replication that occurred in the process of cell division In addition to the aging theory at the gene level, such as the error theory that error proteins are caused by error accumulation, there is the reactive oxygen theory that active oxygen attacks lipids such as DNA, proteins, and cell membranes to cause aging.

In addition, as a means studied to prevent aging, the gene is manipulated to stop the effects of aging, the telomerase gene is introduced to enable infinite growth, and the gene that generates reactive oxygen suppression enzyme is activated. There are gene manipulation such as oxidization and elimination of active oxygen by supplementation with antioxidants and SOD-like active substances. However, genetic engineering and genetic recombination methods have safety problems, and aging is entangled with various factors, so it is difficult to achieve effects at the individual level with methods that focus on specific factors. .
WO01 / 088149 Patent publication 2003-508079

  Since an individual is composed of cells, aging at the cellular level results in aging of the individual. Therefore, the present invention prevents cells from aging without performing genetic manipulation, and without eliminating the active oxygen by supplementing antioxidant substances or SOD-like active substances, and further, tissues, organs, organs composed of cells An object of the present invention is to provide an effective new ingredient for preventing aging of an individual.

  The inventors of the present invention have been researching various plant components, mainly rice, which is a staple food, from the viewpoint of fauna and flora. In the process, rice has been found to have many possibilities and benefits that could not have been predicted before. Therefore, we have taken up the theme of rice, which is used as a staple food and has proven to be the safest, and has conducted comprehensive rice research. As one of the themes, we have conducted intensive research on aging prevention. In the process, we have found that rice contains ingredients that exhibit anti-aging effects when taken orally, and complete the present invention. It came to.

  In the present invention, it has not yet been elucidated as to what component derived from rice (or a combination of a plurality of components or a combination of a plurality of components at a predetermined abundance ratio) has an anti-aging effect. It has been found that, when orally ingested using rice as a raw material, it exhibits anti-aging effects on living organisms or individuals, organs, organs, tissues, body fluids or cells, gene functions, etc. . It has also been found that the anti-aging agent according to the present invention has anti-aging, aging improvement, youth retention and rejuvenation actions. Although the mechanism of action of the anti-aging effect is not clear, it is considered that it is a non-SOD-like active action that does not depend on the active oxygen scavenging action or SOD-like activity.

  That is, the present invention (1) is an antiaging agent for oral use containing pulverized rice as an active ingredient.

  The present invention (2) is an oral antiaging agent containing rice extract as an active ingredient.

  The present invention (3) is an antiaging agent for oral use containing, as an active ingredient, a rice hydrolyzate that has been subjected to enzymatic degradation or koji action.

  The present invention (4) is an antiaging agent for oral use containing, as an active ingredient, an enzyme that has been subjected to enzymatic degradation or koji before, during or after extraction of rice.

  The present invention (5) is an antiaging agent for oral use containing, as an active ingredient, a product obtained by subjecting an extract of rice or enzymatic degradation or koji to alcohol fermentation or organic acid fermentation.

  According to the present invention, it is possible to prevent aging, improve aging, maintain youthfulness, and rejuvenate living organisms or individuals, organs, organs, tissues, body fluids or cells. Therefore, aging skin, senile xeroderma, skin aging symptoms (wrinkles, reduced elasticity, sagging, blemishes, color change, dryness), decreased memory, learning ability, muscle weakness, weakness, endocrine Decline in system function, visual acuity, hearing loss, dull reflexes, slow response to external stimuli, until changes in the body caused by external stimuli return to their original state It has the effect of preventing and improving the deterioration of physiological functions such as taking time, worsening the healing of wounds, decreasing immune function, and decreasing resistance to viruses and bacteria. It also has the effect of preventing and improving various diseases that develop due to aging.

  In addition, rice has been a staple food so far, and new uses have hardly been developed. In addition, rice has been a staple food and has been proven to be safe. That is, the present invention has been found not only to have an excellent anti-aging effect for oral aging, but also has been well-proven safety, various drugs, quasi drugs, health It can be said that it is a very significant invention that can be used for foods and foods. In addition, it is extremely meaningful that we have found new uses for rice and are able to expand consumption by improving the image of rice.

  “Rice” according to the present invention is a concept that includes not only rice such as white rice, brown rice and germinated rice, but also part of rice such as white rice and red rice bran. However, it is preferable to use one that essentially contains a white rice portion, that is, white rice, brown rice, germinated rice, or white birch. Here, with respect to white rice and brown rice, it refers to brown rice and white rice such as glutinous rice and glutinous rice regardless of japonica and indica rice. In addition, white rabbit and red rabbit generally refer to 92% or more red rabbit or 92% or less white rabbit that appears at the time of whitening, but a mixture of both may be used. In addition, since the active ingredient is stable to heat and light, the above-mentioned raw materials are dipping, steaming, roasting (pointing to all of sand roasting, net roasting, hot air roasting, etc.), steaming roasting, freeze drying It may be subjected to raw material treatment such as surface modification such as UV irradiation, photo-modification such as UV irradiation, pressure roasting such as Patrice, frying. White rice, brown rice, and germinated rice are effective when used as they are, but are preferably pulverized for practical use. In order to pulverize white rice, brown rice and germinated rice into powder, a general method may be used using a pulverizer or a rice mill.

  In the case of producing germinated rice, germinated rice is immersed in water or sprayed with water. The temperature at the time of germination is 5-70 degreeC. However, the temperature and time are not limited as long as germination occurs. In addition, when there is a risk of water rot during germination, it is preferable to replace the water so that it does not rot or to perform some preservative. Here, germination refers to everything from just before germination to germination. The germinated rice is washed thoroughly before use. At this time, you may dry and use. When rice is extracted, or enzymatically decomposed or subjected to koji, the raw material rice is pulverized into granules or powders, so that the surface area is increased and the efficiency is improved. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the rice tissue.

  When water is extracted from rice, a high extraction temperature is efficient, but extraction can be sufficiently performed even at a low temperature. However, in the case of a low temperature of 40 ° C. or lower, it is desirable that the pH is made acidic or alkaline, or a preservative or alcohol is added so that the rice is not spoiled. The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined by the extraction temperature. The extraction may be performed under pressure, normal pressure, or reduced pressure. In the case of water extraction, the most serious problem is the gelatinization phenomenon. If it becomes paste-like, not only extraction efficiency will worsen but it will be extremely difficult in actual work. In order to prevent this, the reaction may be performed by adding amylase or acidifying with hydrochloric acid or the like to cut the starch. By using this method, the problem can be solved sufficiently and there is no problem in practical use.

  It is also effective to perform acid decomposition extraction or alkali decomposition extraction because the active ingredient in the extract is stable to acid and alkali. In this case, neutralization and desalting are performed as necessary. Also when extracting with an organic solvent, it is desirable to extract rice by pulverizing or pulverizing it as much as possible. The organic solvent may be a common organic solvent such as alcohol, acetone, n-hexane, methanol, etc., but those that are harmful to the human body should be safe because the solvent must be completely removed after extraction. . In addition, rice may be subjected to oxygen decomposition or rice bran. As used herein, oxygen degradation means one or more enzymes that act on rice, such as starch degrading enzymes (liquefaction enzymes, saccharifying enzymes), proteolytic enzymes, lipolytic enzymes, fiber degrading enzymes, lignin degrading enzymes, and pectin degrading enzymes. It means to act. For example, the combination of a liquefying enzyme and a saccharifying enzyme can be mentioned. In addition, the type of koji mold and the variety and type of rice are not questioned.

  Furthermore, in performing the said extraction, you may make said enzyme decomposition | disassembly and soot act before extraction, simultaneous with extraction, or after extraction. In the present invention, when the above-described treatment is further performed, or when the organic acid fermentation such as alcohol fermentation, lactic acid fermentation, or acetic acid fermentation is performed simultaneously or after the treatment, the following points are also effective. When alcoholic fermentation is performed, concentration is easy and concentration of active ingredients becomes easy. Sugar may be removed by aeration fermentation with yeast, alcohol precipitation, or the like.

When the oral antiaging agent according to the present invention is used, aging of living organisms and aging of cells are prevented. Here, the living organism refers to a multicellular organism (including higher animals such as mammals including humans). In addition, the cell is not limited to a single cell, but is also a concept including organs, organs, tissues, body fluids (fluids such as blood and lymph), and the like that are composed of a plurality of cells. In addition, “oral” means an administration route other than transdermal (skin application). The routes of administration other than transdermal, oral administration, and other oral (gavage, intraduodenal). Since the skin is covered with the stratum corneum, the component is difficult to be absorbed by transdermal administration, but by being administered by these routes, the active ingredient reaches the intended site of action regardless of the whole body or the local area, The effect can appear. The term “anti-aging” means prevention of aging, improvement of aging, maintenance of youthfulness, rejuvenation, for example, prevention and improvement of deterioration of physiological function, as a specific example, a living organism or an individual, Prevention of aging of organs, organs, tissues, body fluids or cells, improvement of aging, retention of youthfulness, rejuvenation, more specifically, aging skin, senile xerosis, skin aging symptoms (wrinkles, reduced elasticity, elasticity, Sagging, spots, color change, dryness), memory loss, learning ability decline, muscle weakness, physical strength decline, endocrine function decline, vision loss, hearing loss, reflexes become dull, external Slow response to stimuli, changes in the body caused by external stimuli take time to return to the original state, wound healing becomes poor, immune function declines, resistance to viruses and bacteria Against decline in physiological function Prevention, may be mentioned improvement. Furthermore, it has preventive and ameliorating effects on various diseases caused by aging. Furthermore, the anti-aging agent according to the present invention directly acts on the dermis from the body to the dermis, and the anti-aging effect of the dermis can be obtained.

  The product of the present invention obtained as described above is used as it is, or after being squeezed and filtered without separating the residue. When it is used as it is, it is sterilized or sterilized to make a product. The product of the present invention is aerosol, liquid, jelly, elixir, capsule, granule, pill, suspension, emulsion, powder, fine granule, tablet, syrup, injection depending on the actual application. It can also be used in dosage forms such as agents, troches, and limonades. Furthermore, there may be a prescription added to foods and beverages. In addition, you may add another compounding component and a chemical | medical agent according to a use. In addition, although the addition amount of the product of the present invention depends on the kind of the drug to be added, food and drink, etc., generally 0.0001 to 90 parts by weight of the product extract of the present invention per 100 parts by weight of the product is preferable. Is added in an amount of 0.01 to 60 parts by weight, most preferably 0.1 to 30 parts by weight. The intake of the product of the present invention is 0.0001 to 100 ml per day, preferably 0.001 to 50 ml, most preferably 0.01 to 20 ml per kg body weight.

  The oral antiaging agent according to the present invention is useful for various specific uses. For example, prevention of aging of living organisms or individuals, organs, organs, tissues, cells, improvement of aging, retention of youthfulness, rejuvenation; prevention of aging of cell activity, cell cycle, gene function, improvement of aging, retention of youthfulness, Rejuvenation; cardiovascular system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue / organ, spleen, thymus), skin, skin appendages, digestive system (lip, tonsils, teeth, esophagus, stomach) , Small intestine, large intestine, liver, bile duct system, spleen), respiratory system (nose, pharynx, larynx, organ, lung), urinary system (kidney, urinary tract), sex breeding system (male genital organ, female genital organ), endocrine system (Pituitary gland, pineal gland, thyroid gland, adrenal gland), brain, nervous system (peripheral nerve, central nervous system), metabolic system (sugar, lipid, uric acid metabolism), balance / hearing apparatus, visual instrument, each mucous system, motor organ (Bone, joints, muscles, tendons, ligaments) and other aging prevention, aging improvement, youthfulness, rejuvenation Door can be. In relation to the actual product, for example, an indication that it is used for preventing aging is attached to a package (for example, drug, cosmetics, food and drink), a brochure, an instruction manual, etc. Is preferred. By attaching such a display, it is obvious to the user that the product of the present invention has such an effect. Here, the “indication to be used for the prevention of aging” includes not only the case where the aging prevention is specified but also a display suggesting the aging prevention.

Example 1
The white rice was put into a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating up and cooling, solid-liquid separation was performed using the filter and 2.5 L of filtrate was obtained. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 2
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 250 g of candy and 3 L of water were added and left at 55 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.6 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 3
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of starch-degrading enzyme and 3 L of water were added and left at 55 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 4
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of proteolytic enzyme and 3 L of water were added and allowed to stand at 50 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.3 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 5
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of a fiber-degrading enzyme and 3 L of water were added and left at 50 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.2 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 6
The white rice was put into a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of lipolytic enzyme and 3 L of water were added and left at 50 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.1 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 7
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.6 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 8
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating up and cooling, 5 g of proteolytic enzymes were added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 9
The white rice was put into a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of lipolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 10
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating up and cooling, 5 g of fiber-degrading enzymes were added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 11
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 3 L of 1 / 10N hydrochloric acid was added, stirred well and allowed to stand for 24 hours. Thereafter, solid-liquid separation was performed using a filter and neutralized with caustic soda to obtain 2.3 L of an extract. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 12
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 3 L of 95% ethanol was added, stirred well and left for 4 days. Thereafter, solid-liquid separation was performed using a filter to obtain a 2 L extract. 4 L of water was added to the filtrate, and ethanol was distilled off with a rotary evaporator to 2 L. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 13
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. After cooling, yeast was added and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 14
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, the mixture was sterilized by heating, cooled, added with lactic acid bacteria, and fermented at 37 ° C. for 2 days. Thereafter, solid-liquid separation was performed using a filter to obtain 2.3 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Example 15
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, 150 mL of 95% ethanol and acetic acid bacteria were added, and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 2.4 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.
Test example 1
Since an individual is composed of cells, aging of each cell results in aging changes of the individual. Therefore, the anti-aging effect of the product of the present invention was verified at the cell level.
Method: Intestinal mucosal epithelial cells (IEC-6) cultured confluently were cultured in a culture medium supplemented with a 1% sample for 30 days. The medium was not changed after the sample was added.
Sample: Example 1 to Example 15. In Example 13, the sample was concentrated under reduced pressure using a rotary evaporator, the alcohol content was distilled off, water was added, and the amount returned to the original amount was used as a sample.
Results: As a result of macroscopic observation of IEC-6 after 30 days of culture, no abnormalities were observed in cells cultured in a medium supplemented with 1% of the product of the present invention, and the cells remained normal and continued to survive. Turned out to be. The cells were young. On the other hand, the cells to which the product of the present invention was not added could not continue to survive. From the above tests, the effects of the present invention on preventing aging and maintaining youthfulness were proved at the cellular level.
Test example 2
Test Example 1 proved the anti-aging effect of the product of the present invention on cells. Then, the anti-aging effect of the whole individual | organism | solid was investigated using the experimental animal next.

  In general, changes in the state of the body can be known by measuring changes in heat generated from animals. There is a daily rhythm in the amount of heat released (nocturnal rhythm), and nocturnal mice have a large amount of outflow at night and a small amount during the day. It is said that the difference in calorific value between night and day is large and the fluctuation in the daytime is clear. The level of this rhythm (the night-day difference being near zero) is an indicator of aging of the whole individual. In addition, the total amount of heat decreases with aging. Therefore, using an SAM mouse that is known to accelerate the aging phenomenon, the effect of preventing the aging of the product of the present invention was examined using changes in calorific value and changes in the general state as indices.

The animal SAM mice used are genetically short-lived strains, and the period until adulthood is the same as that of normal mice, but loss of skin gloss, increased skin roughness, loss of hair, immunity It is known that aging phenomena such as functional decline, auditory function decline, and brain atrophy progress rapidly, and that learning ability and memory ability decline with aging. In particular, since it is said that aging clearly appears between 26 and 35 weeks, measurements and observations were made during the period of 15 to 35 weeks of age.
Method: Five 9-week-old male SAM mice were preliminarily raised for 6 weeks in a state in which normal food and drinking water were freely available. Three of the five animals were fed up to 35 weeks of age after receiving a food containing the product of the present invention after preliminary breeding. The remaining two were fed normal food and were raised to 35 weeks of age. During the period from 15 weeks of age to 35 weeks of age, the calorific value of the mouse was measured using a calorimeter, and the general state was observed visually. In the calorimeter, enough water and food were prepared, a rotating wheel was set up to exercise, and a window was provided to distinguish day and night.
Sample: Example 14
Results: As a result of the long-term breeding test, the SAM mice (invention group) fed with the diet of the present invention have a large difference in the calorific value between night and day at any time from 15 to 35 weeks of age. The circadian variation was clear and the circadian rhythm, which is an index of aging, was not flattened (Fig. 1). In addition, compared with the 15-week-old before administration, the diurnal variation became clearer at 19-35 weeks after administration. Moreover, the total amount of heat per day also gradually increased (FIG. 2), and was significantly higher than that of the SAM mice (control group) fed with normal food. On the other hand, in the control group, the flattening of the daily rhythm was remarkably observed at 23 to 35 weeks of age, and the total heat amount of the day also decreased with time. From this, it was found that the product of the present invention has the effect of preventing and improving the aging of the whole individual and maintaining the youthfulness and rejuvenation.

  When the general state was observed, the control group was injured in the tail whenever it was put into the calorimeter from the age of 28 weeks. One of the causes was that the tail was pinched by the wire mesh when turning the rotating wheel, but this was thought to be due to the deterioration of motor ability, reflexes, visual acuity, and blur due to aging. On the other hand, in the product group of the present invention, no tail injury was observed, and no changes in aging such as motor ability, reflexes, visual acuity, and blur were observed from the state of motion on the rotating wheel.

  Further, at the age of 35 weeks, in the control group, the skin was not glossy, rough, and the hair was thin, but in the product group of the present invention, no change in aging of the skin and hair was observed.

  As described above, it was proved that the product of the present invention prevents the aging of the whole body from the daily rhythm of the calorific value and the change of the total calorific value, which are indicators of aging of the whole individual. As a result of observation, it was confirmed that aging changes of skin and hair, motor ability and reflexes, deterioration of visual acuity, and aging changes such as blur were prevented. Furthermore, it was considered that the product of the present invention prevents immunity, auditory function, brain atrophy, learning ability, and memory ability, which are characteristic of aging in SAM mice.

  Further, since the product of the present invention has the effect of preventing and improving the aging of the whole individual and maintaining the youthfulness and the rejuvenation effect, the anti-aging and rejuvenation effect of the cells proved in Test Example 1 is the same as the intestinal mucosal epithelial cells used in the test. It was found that the same effect was exhibited not only in other cells.

  From Test Examples 1 and 2, the product of the present invention has aging prevention, improvement, rejuvenation retention, rejuvenation effect of individuals, organs, organs, tissues, cells, etc., aging prevention of gene function, aging improvement, youthfulness retention, It became clear that it had a rejuvenating effect.

FIG. 1 is an examination of how much the anti-aging effect according to the present invention (Example 14) affects the circadian rhythm according to Test Example 2. FIG. 2 is a graph showing how the anti-aging effect according to the present invention (Example 14) affects the total amount of heat in mice according to Test Example 2.

Claims (1)

Oral anti-aging agent for suppressing flattening of circadian rhythm and preventing a decrease in the total calorific value of the day, containing as an active ingredient the processed pulverized white rice with liquefaction enzyme and saccharification enzyme, followed by lactic acid fermentation .

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