JP5102432B2 - Cancer cell dormant - Google Patents

Description

  The present invention relates to a cancer cell dormant (for example, colorectal cancer) and a colorectal cancer preventive or therapeutic agent comprising a rice-derived component as an active ingredient.

  Cancer accounts for about 30% of Japanese causes of death, and overcoming cancer is a national issue. The current treatment is aimed at the removal and extinction of cancer, but the method varies depending on the progression.

  Systemic treatment (anticancer drug treatment) is performed for advanced cancer, terminal cancer, and cancer that has recurred or metastasized after treatment. However, since conventional anticancer agents do not act specifically on cancer but mostly act on fast-growing cells, even normal cells can be bone marrow, digestive tract mucosa, hair Such proliferating cells are affected by anticancer drugs and cause side effects such as leucopenia, vomiting, diarrhea, and hair loss. In addition, since it is necessary to administer a large amount of anticancer drugs to eliminate the cancer, if the whole body is not in a state where it can be completely free from side effects, the condition worsens or the life span is shortened. Invite. Even if the dose is controlled and the cancer is successfully reduced, a certain degree of life-prolonging effect can be expected. Thus, there is currently no effective drug that can overcome the cancer that has reached the above stage. Therefore, there is a need for a drug that can prevent cancer progression and metastasis and can be taken for a long time without side effects. It has been.

  On the other hand, if cancer is detected at an early stage and is localized, local treatment (surgical treatment, radiotherapy) is performed, but metastasis to lymph nodes etc. is possible even if it can be excised and disappeared macroscopically. Since there is a high possibility of recurrence in some cases, there is a need for drugs that are effective in preventing cancer recurrence.

  Furthermore, deaths from colorectal cancer among cancers are ranked third after lung cancer and stomach cancer, and have been increasing in recent years. As a cause of colorectal cancer, it has been pointed out that the intake of animal fat is increased due to the westernization of eating habits. In terms of age, there are many in the order of 60s, 50s, and 70s, and there is a tendency for elderly people. In the future, colon cancer is expected to increase more and more with the westernization of eating habits and the increasing number of elderly people.

Colorectal cancer can be completely cured by endoscopic resection and surgical treatment if detected early. However, since there is generally no subjective symptom, it is difficult to detect at the time of asymptomatic unless it is regularly examined. Even if it is detected and resected early, adjuvant therapy aimed at preventing recurrence is necessary, but there is currently no effective anticancer drug having the effect of preventing recurrence. For this reason, the current situation is that after surgery, a relapse check is performed every few months, and it can only be detected early.
JP 2002-121132 A

  It is said that it usually takes 10 years for one cancer cell to grow and develop cancer. If the growth rate doubles, it will develop in 5 years. Conversely, if it can be delayed by 1/10, it will take 100 years to develop, and the lifespan can be completed without developing cancer. Similarly, when cancer develops or metastasizes, if the growth of cancer cells can be delayed, the progression of the disease can be maintained. An object of the present invention is to provide a safe cell dormant that is excellent in the effect of delaying or inhibiting the growth of cancer cells and has no side effects even when used for a long period of time. The purpose is to provide preventive and therapeutic agents.

  The present inventor has worked on various product development using a natural material called rice, and as a result of intensive research, some components contained in the rice have a dormant action of cancer cells, and prevention of colorectal cancer. The present invention has been completed by finding that it is effective for treatment.

  The present invention (1) is a cancer cell dormant containing a ground rice product as an active ingredient.

  The present invention (2) is a cancer cell dormant containing rice extract as an active ingredient.

  In the present invention (3), the extract may be obtained by adding water or an organic solvent to rice, treating rice with an acid or an alkali, causing rice hydrolyzate to act on an enzyme or koji, or these Is the cancer cell dormant of the invention (2), which is carried out under heating or non-heating.

  The present invention (4) is a cancer cell dormant containing, as an active ingredient, a substance to which an enzyme or koji is acted before, simultaneously with or after extraction of rice.

  The present invention (5) is a cancer cell dormant containing, as an active ingredient, a product obtained by subjecting an extract of rice or an enzyme or koji to the rice extract to alcohol fermentation or organic acid fermentation.

  The present invention (6) is the cancer cell diapausing agent according to any one of the inventions (1) to (5), wherein the cancer cells are colon cancer.

  The present invention (7) is a preventive / therapeutic agent for colorectal cancer containing a ground rice product as an active ingredient.

  The present invention (8) is a preventive / therapeutic agent for colorectal cancer containing rice extract as an active ingredient.

  In the present invention (9), the extract may be prepared by adding water or an organic solvent to rice, treating rice with an acid or alkali, allowing rice hydrolyzate to act on an enzyme or koji, or these Is the preventive or therapeutic agent for colorectal cancer according to the invention (8), which is carried out under heating or non-heating.

  The present invention (10) is a preventive / therapeutic agent for colorectal cancer containing, as an active ingredient, a substance to which an enzyme or koji is acted before, simultaneously with or after extraction of rice.

  The present invention (11) is a colorectal cancer preventive / therapeutic agent containing as an active ingredient a product obtained by subjecting an extract of rice or an enzyme or koji to the rice extract to alcohol fermentation or organic acid fermentation.

Here, the meaning of each term in this specification is explained. The “cancer cell dormant” refers to a drug having an action of delaying or suppressing the growth of cancer cells. Through this action, it is possible to prevent the onset, progression, metastasis, and recurrence of cancer. Moreover, the kind of "cancer" is not specifically limited, For example, colon cancer can be mentioned. “Extraction” means physical treatment (eg, pressing, heat treatment), chemical treatment (eg, addition of water or organic solvent, acid or alkali treatment, liquid CO ₂ treatment), biological (biochemical) treatment. (For example, wrinkle, microbial treatment, enzyme treatment) is applied alone or in combination.

  Hereinafter, the best mode for carrying out the present invention will be described in detail. The essence of the present invention is materials and applications, and other elements (for example, dose, dosage form, mechanism of action, etc.) do not limit the present invention.

  First, “rice” according to the present invention is a concept including not only rice such as white rice, brown rice and germinated rice, but also part of rice such as white rice and red rice bran. However, it is preferable to use one that essentially contains a white rice portion, that is, white rice, brown rice, germinated rice, or white birch. Here, with respect to white rice and brown rice, it refers to brown rice and white rice such as glutinous rice and glutinous rice regardless of japonica and indica rice. In addition, white rabbit and red rabbit generally refer to 92% or more red rabbit or 92% or less white rabbit that appears at the time of whitening, but a mixture of both may be used. However, those containing 92% to 30% of white birch, such as 92% to 50%, 80% to 60%, 60% to 30%, etc., are suitable. In addition, since the active ingredient is stable to heat and light, the above-mentioned raw materials are dipping, steaming, roasting (pointing to all of sand roasting, net roasting, hot air roasting, etc.), steaming roasting, freeze drying It may be subjected to raw material treatment such as surface modification such as UV irradiation, photo-modification such as UV irradiation, pressure roasting such as Patrice, frying. White rice, brown rice, and germinated rice are effective when used as they are, but are preferably pulverized for practical use. In order to pulverize white rice, brown rice and germinated rice into powder, a general method may be used using a pulverizer or a rice mill.

  In the case of producing germinated rice, germinated rice is immersed in water or sprayed with water. The temperature at the time of germination is 5-70 degreeC. However, the temperature and time are not limited as long as germination occurs. In addition, when there is a risk of water rot during germination, it is preferable to replace the water so that it does not rot or to perform some preservative. Here, germination refers to everything from just before germination to germination. The germinated rice is washed thoroughly before use. At this time, you may dry and use. When rice is extracted, or enzymatically decomposed or subjected to koji, the raw material rice is pulverized into granules or powders, so that the surface area is increased and the efficiency is improved. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the rice tissue.

  When water is extracted from rice, a high extraction temperature is efficient, but extraction can be sufficiently performed even at a low temperature. However, in the case of a low temperature of 40 ° C. or lower, it is desirable that the pH is made acidic or alkaline, or a preservative or alcohol is added so that the rice is not spoiled. The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined by the extraction temperature. The extraction may be performed under pressure, normal pressure, or reduced pressure. In the case of water extraction, the most serious problem is the gelatinization phenomenon. If it becomes paste-like, not only extraction efficiency will worsen but it will be extremely difficult in actual work. In order to prevent this, amylase may be added and reacted, or the starch may be cut by acidifying with hydrochloric acid or the like. By using this method, the problem can be solved sufficiently and there is no problem in practical use.

  It is also effective to perform acid decomposition extraction or alkali decomposition extraction because the active ingredient in the extract is stable to acid and alkali. In this case, neutralization and desalting are performed as necessary. Also when extracting with an organic solvent, it is desirable to extract rice by pulverizing or pulverizing it as much as possible. The organic solvent may be a common organic solvent such as alcohol, acetone, n-hexane, ethyl acetate, methanol, etc., but those that are harmful to the human body are safe because the solvent must be completely removed after extraction. Things are good. In addition, rice may be subjected to oxygen decomposition or rice bran. As used herein, oxygen degradation means one or more enzymes that act on rice, such as starch degrading enzymes (liquefaction enzymes, saccharifying enzymes), proteolytic enzymes, lipolytic enzymes, fiber degrading enzymes, lignin degrading enzymes, and pectin degrading enzymes. It means to act. For example, the combination of a liquefying enzyme and a saccharifying enzyme can be mentioned. In addition, the type of koji mold and the variety and type of rice are not questioned. In addition, you may carry out combining acid, alkali extraction, organic-solvent extraction, enzymatic decomposition, and anther effect.

  In performing the above extraction, the above enzymatic decomposition and soot may be allowed to act before, simultaneously with or after extraction.

  Further, organic acid fermentation such as alcohol fermentation, lactic acid fermentation, and acetic acid fermentation may be performed simultaneously with or after the above treatment. In particular, it has been confirmed that when organic acid fermentation, particularly lactic acid fermentation, is performed, cancer cell dormancy effect and colon cancer prevention and treatment effect are high. Moreover, when alcoholic fermentation is performed, it is easy to concentrate and the active ingredient is easily concentrated. Sugar may be removed by aeration fermentation with yeast, alcohol precipitation, or the like. The fermentation form may be moromi fermentation or liquid fermentation, but liquid fermentation is preferred. These fermentations may be repeated twice or more, or different fermentation methods may be combined.

  The product of the present invention obtained as described above is used as it is, or after being squeezed and filtered without separating the residue. When it is used as it is, it is sterilized or sterilized to make a product. The product of the present invention is aerosol, liquid, jelly, elixir, capsule, granule, pill, suspension, emulsion, powder, fine granule, tablet, syrup, injection depending on the actual application. It can also be used in dosage forms such as agents, troches, and limonades. Furthermore, there may be a prescription added to foods and beverages. In addition, you may add another compounding component and a chemical | medical agent according to a use. In addition, although the addition amount of the product of the present invention depends on the kind of the drug to be added, food and drink, etc., generally 0.0001 to 90 parts by weight of the product extract of the present invention per 100 parts by weight of the product is preferable. Is added in an amount of 0.01 to 60 parts by weight, most preferably 0.1 to 30 parts by weight. The intake of the product of the present invention is 0.0001 to 100 ml per day, preferably 0.001 to 50 ml, most preferably 0.01 to 20 ml per kg body weight.

  In relation to the actual product, for example, it is preferable to display directly or indirectly a label indicating that it is a cancer cell dormant or a colorectal cancer preventive or therapeutic agent. Examples of objects to which the label is attached include packages of products (for example, drugs, foods for specified health use, foods for patients, health foods, foods and drinks), pamphlets, instruction manuals, and the like. By attaching such a display, it is obvious to the user that the product of the present invention has such an effect. Here, the “indication that it is a cancer cell dormant” or the “indication that it is a colorectal cancer preventive / therapeutic agent” includes not only the case where these are specified, but also the indication suggesting them.

Example 1
The white rice was put into a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating up and cooling, solid-liquid separation was performed using the filter and 2.5 L of filtrate was obtained. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 2
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 250 g of candy and 3 L of water were added and left at 55 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.6 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 3
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of starch-degrading enzyme and 3 L of water were added and left at 55 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 4
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of a fiber-degrading enzyme and 3 L of water were added and left at 50 ° C. for 16 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.0 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 5
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.6 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 6
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating up and cooling, 5 g of proteolytic enzymes were added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 7
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of proteolytic enzyme, 5 g of lipolytic enzyme, 5 g of fiber degrading enzyme, 5 g of starch degrading enzyme, 5 g of pectin degrading enzyme and 3 L of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised, boiling and extracted for 5 minutes, cooled to 30 ° C., and squeezed with a squeezer to obtain 2.6 L of the present invention product.

Example 8
5 g of liquefied enzyme and 3 L of water were added to 1 kg of white lees with a polishing rate of 30 to 60% and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.4 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 9
5 g of liquefied enzyme and 3 L of water were added to 1 kg of white lees with a whitening ratio of 60 to 80%, and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.4 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 10
5 g of liquefied enzyme and 3 L of water were added to 1 kg of white candy with a whitening ratio of 50 to 92% and left at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.3 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 11
5 g of liquefied enzyme and 3 L of water were added to 1 kg of white candy with a polishing rate of 30 to 92%, and the mixture was allowed to stand at 60 ° C. for 4 hours. Thereafter, the temperature was raised by heating, and after cooling, 5 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 2.5 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 12
To 490 g of white koji with a whitening ratio of 50-70% and 10 g of red koji over 92%, add 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1.5 L of water And left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised, boiling and extracted for 5 minutes, cooled to 30 ° C., and squeezed with a squeezer to obtain 1.2 L of the present invention product.

Example 13
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1.5 L of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised, boiling and extracted for 5 minutes, then cooled to 30 ° C., and squeezed with a squeezer to obtain 2.2 L of the present invention.

Example 14
1 kg of rice with germs attached was soaked in water at 30 ° C. for 3 days to absorb water and germinate the rice. The germinated rice was thoroughly washed, dried at 50 ° C. for 24 hours, and then finely pulverized to obtain a pulverized product of germinated rice. To 500 g of the pulverized rice flour, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1.5 L of water were added and left at 50 ° C. for 20 hours. Thereafter, the temperature was gradually raised, boiling and extracted for 5 minutes, then cooled to 30 ° C., and squeezed with a squeezer to obtain 1.1 L of the present product.

Example 15
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 3 L of 1 / 10N hydrochloric acid was added, stirred well and allowed to stand for 24 hours. Thereafter, solid-liquid separation was performed using a filter and neutralized with caustic soda to obtain 2.3 L of an extract. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 16
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 3 L of 95% ethanol was added, stirred well and left for 4 days. Thereafter, solid-liquid separation was performed using a filter to obtain a 2 L extract. 4 L of water was added to the filtrate, and ethanol was distilled off with a rotary evaporator to 2 L. This was heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 17
White rice was ground in a grinder to obtain 1 kg of white rice grind. To this pulverized product, 5 g of liquefied enzyme and 3 L of water were added and left at 60 ° C. for 4 hours. Then, after heating and heating and cooling, 5 g of starch-degrading enzyme was added and allowed to stand at 55 ° C. for 4 hours. After heat sterilization and cooling, lactic acid bacteria were added, and fermentation was performed at 37 ° C. for 2 days. This was squeezed with a squeezer, separated into solid and liquid, and heated at 85 ° C. for 30 minutes to obtain the product of the present invention.

Example 18
The liquid 2.5L obtained in Example 1 was sterilized by heating, and after cooling, lactic acid bacteria were added, followed by fermentation at 37 ° C. for 2 days. Thereafter, the mixture was filtered using a filter and heated at 85 ° C. for 30 minutes to obtain 2.4 L of the present product.

Example 19
Yeast was added to 2.6 L of the liquid obtained in Example 7, and fermentation was performed for 15 days. Thereafter, the mixture was filtered using a filter and heated at 85 ° C. for 30 minutes to obtain 2.5 L of the present product.

Example 20
To 2.5 L of the filtrate obtained in Example 11, 150 mL of 95% ethanol and acetic acid bacteria were added, and acetic acid fermentation was performed for 20 days. Thereafter, the mixture was filtered using a filter and heated at 85 ° C. for 30 minutes to obtain 2.4 L of the present product.

Test example 1
Method: 1 × 10 ⁵ human colon cancer mucosal cell HCT116 strain was seeded, and each sample having a concentration of 10 ⁻¹ of the stock solution was added to the medium and cultured for 72 hours, and its growth was observed. As a control, 1 × 10 ⁵ cells were inoculated using human colon cancer mucosal cell line HCT116 and cultured for 72 hours in a medium to which no sample was added.
Sample: Examples 1-7, 9, 11, and 15-20
Results: FIG. 1 shows colon cancer mucosa cells HCT116 after 72 hours of culture in Example 18 as a representative example of control and sample. In control colon cancer mucosal cells HCT116, dividing cells were observed after 72 hours of culture. On the other hand, in colon cancer mucosal cells HCT116 in which Examples ¹ to 7, 9, 11, and 15 to 20 were added to the culture solution at a concentration of 10 ⁻¹ , dividing cells were not observed after 72 hours of culturing. Antiproliferative action was observed. As a result, it was found that the present invention has an effect of preventing and treating colorectal cancer. It has also been found to have an action of delaying or suppressing the growth of cancer cells, and through this action, the onset, progression, metastasis, and recurrence of cancer are prevented.

Test example 2
Method: Human colorectal cancer mucosal cell line HCT116 was used, and each sample having a stock concentration of 10 ⁻¹ was added to the medium and cultured for 24 hours. The BrdU incorporation was quantified, and DNA synthesis was measured using ELISA. As a control, human colon cancer mucosal cell line HCT116 was used and cultured for 24 hours in a medium to which no sample was added.
Sample: Examples 1-7, 9, 11, and 15-20
Results: By adding Examples 1-7, 9, 11, and 15-20, the DNA synthesis | combination of the human colon cancer mucosa cell was significantly suppressed compared with control (FIG. 2). As a result, it has been found that the present invention inhibits the growth of cancer cells by suppressing the DNA synthesis of the cancer cells. In particular, it can be seen that the effects of the examples using liquefaction enzymes (Examples 1, 5, 6, 9, 11, 17 and 20) and the examples of performing lactic acid fermentation (Examples 17 and 18) are remarkable. .

Test example 3
Method: Using human colon cancer mucosal cell line HCT116, culturing for 24 hours after adding each sample at 10 ^-1 concentration of the stock solution in the medium, and quantifying cytotoxicity by measuring LDH in the culture supernatant did. As a control, human colon cancer mucosal cell line HCT116 was used and cultured for 24 hours in a medium to which no sample was added.
Sample: Examples 1-7, 9, 11, and 15-20
Results: As a result of measuring LDH in the supernatant, no significant difference was observed between the controls in which Examples 1 to 7, 9, 11, and 15 to 20 were added (FIG. 3). As a result, it has been found that the cancer cell proliferation inhibitory effect of the present invention is not due to cytotoxicity.

FIG. 1 is a photograph of colon cancer mucosal cells HCT116 taken 72 hours after culture when Control (A) and Example 18 (B) were added in Test Example 1. FIG. 2 is a graph showing the amount of DNA synthesized in human colon cancer mucosal cells when Test and Examples 1 to 7, 9, 11, and 15 to 20 were added in Test Example 2. FIG. 3 is a diagram showing LDH activity in the culture supernatant when Control and Examples 1 to 7, 9, 11, and 15 to 20 were added in Test Example 3.

Claims (21)

A colon cancer cell growth inhibitor comprising pulverized white rice as an active ingredient . A colon cancer cell growth inhibitor comprising a white rice extract as an active ingredient . The extract is obtained by adding water or an organic solvent to white rice, processed white rice with acid or alkali, processed white rice hydrolyzate with enzyme or koji, or heated or unheated. The agent according to claim 2, which has been performed . An agent for inhibiting the growth of colorectal cancer cells, which contains , as an active ingredient, an enzyme or koji that acts upon extraction of white rice before, simultaneously with or after extraction . A colon cancer cell growth inhibitor comprising, as an active ingredient, a white rice extract or a product obtained by subjecting an extract or a rice cake to alcohol or organic acid fermentation . A colon cancer cell growth inhibitor comprising, as an active ingredient, a liquefied enzyme-treated product made from white rice . A growth inhibitor of colorectal cancer cells, which contains , as an active ingredient, a lactic acid fermentation product made from white rice . A DNA synthesis inhibitor for colorectal cancer cells, comprising a milled white rice product as an active ingredient . A DNA synthesis inhibitor for colorectal cancer cells, comprising an extract of white rice as an active ingredient . The extract is obtained by adding water or an organic solvent to white rice, processed white rice with acid or alkali, processed white rice hydrolyzate with enzyme or koji, or heated or unheated. The agent according to claim 9, which has been performed . An agent for inhibiting DNA synthesis of colon cancer cells, containing , as an active ingredient, an enzyme or koji that acts upon extraction of white rice before, simultaneously with or after extraction . A colon cancer cell DNA synthesis inhibitor comprising, as an active ingredient, an extract of white rice or an enzyme or koji that has been subjected to alcohol fermentation or organic acid fermentation . A DNA synthesis inhibitor for colon cancer cells, containing as an active ingredient a liquefied enzyme-treated product made from white rice . A DNA synthesis inhibitor for colorectal cancer cells, which contains , as an active ingredient, a lactic acid fermentation product made from white rice .   A cytotoxic agent for colorectal cancer cells, comprising a milled product of white rice as an active ingredient.   A cytotoxic agent for colorectal cancer cells, comprising an extract of white rice as an active ingredient.   The extract is obtained by adding water or an organic solvent to white rice, processed white rice with acid or alkali, processed white rice hydrolyzate with enzyme or koji, or heated or unheated. The agent according to claim 16, which has been performed.   A cytotoxic agent for colorectal cancer cells, which contains, as an active ingredient, an enzyme or koji that acts upon extraction of white rice before, simultaneously with or after extraction.   A cytotoxic agent for colorectal cancer cells, comprising, as an active ingredient, an extract of white rice or an enzyme or koji that has been subjected to alcohol fermentation or organic acid fermentation.   A cytotoxic agent for colorectal cancer cells, comprising as an active ingredient a liquefied enzyme-treated product made from white rice.   A cytotoxic agent for colorectal cancer cells, which contains, as an active ingredient, a lactic acid fermentation product made from white rice.