JP2011000055A - Composition for lasting feeling of fullness, containing insoluble dietary fiber derived from grain plant seed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a safe and new material that lasts feeling of fullness after ingestion by taking the material as it is or adding the material to a food and beverage and taking the food and beverage.SOLUTION: There are provided the composition containing an insoluble dietary fiber obtained by treating seeds of grain plant or its germinated young seeds with an enzyme, and the food and beverage and the agent for lasting feeling of fullness each containing the composition.

Description

  The present invention relates to a composition for sustaining satiety containing insoluble dietary fiber derived from plant seeds and germinated seedlings thereof, and a method for producing the same. The present invention also relates to a diet food and drink and a satiety-retaining agent comprising the above composition.

  In recent years, obesity has been increasing. On the other hand, interest in the metabolic syndrome has increased, and there is a great interest in dieting. The basic method of dieting is to limit food intake and prevent excessive caloric intake. Problems with this dietary restriction include the fact that hunger always persists, affecting the vitality of life, and rebounding by quitting food restriction without being able to put up with hunger. It is easy.

  In order to solve these problems, it is important to suppress hunger when dietary intake is restricted. It is easy to expect that effective food intake restriction will be possible by taking food that has a good stomach in a normal diet.

  To date, many reports have been made on such foods that have a good stomach. For example, Patent Document 1 discloses a diet cookie that contains a large amount of agar or carrageenan and unconsciously reduces the amount of food intake, which can provide sufficient texture and fullness when eaten.

Patent document 2 discloses a food composition having an accelerated satiety effect comprising an alginate having an L-guluronic acid content of at least 60% of the total uronic acid units in the alginate.
Patent Document 3 discloses a wrinkle that contains a lump gel of specific dietary fiber and has a chewing feeling and a feeling of fullness.

Patent Document 4 discloses a belly holding agent characterized by containing hydroxypropyl starch and the like.
Patent Document 5 discloses an edible composition using whey protein that stimulates cell secretion of cholecystokinin and glucagon-like peptides, which are satiety peptides.

  Patent Document 6 is a diet solid food mainly composed of 10-50% by weight of dietary fiber consisting of rice bran and 30-80% by weight of fructose. By ingesting with a glass of water, fructose is insulin. It is disclosed that it is absorbed quickly without inducing secretion and increases blood glucose level, thereby reducing hunger and appetite.

JP2005-341890 Special table 2007-533297 JP2000-50819 JP2008-259464 JP2005-538704 JP 11-32728

  An object of this invention is to provide the safe and novel material which can maintain the feeling of fullness after ingestion by ingesting as it is or adding it to food-drinks. Moreover, an object of this invention is to provide the diet food / beverage products and satiety feeling sustaining agent containing this raw material.

  As a result of repeated studies to solve the above-mentioned problems, the present inventors have removed starch from the seeds of cereal plants, preferably gramineous plants, and insoluble after subjecting the remaining peels and seed coats to hemicellulase treatment. The insoluble fraction containing dietary fiber was found to have a satiety-sustaining effect, and the present invention was completed. The gist of the present invention is as follows.

(1) The following steps: (a) preparing the raw material by grinding the seeds of the cereal plant or by collecting the outer seed fraction produced by scouring; (b) A step of removing starch to prepare a starch-removed fraction; (c) a step of enzymatically treating the fraction prepared in step (b) with an enzyme having hemicellulase activity; and (d) an insoluble fraction from the enzyme-treated solution. A composition for sustaining satiety, comprising insoluble dietary fiber derived from the seeds of a cereal plant produced by a treatment comprising a step of recovering sucrose.
(2) The composition according to (1) above, wherein the cereal plant is a gramineous plant.
(3) The composition according to (2) above, wherein the gramineous plant is rice, barley, rye or wheat.
(4) The composition according to (1) above, wherein in the step (b), the starch removal treatment is performed by an enzyme treatment using amylase or glucoamylase.
(5) The composition according to (1) above, wherein in step (b), the starch removal treatment is carried out by a gelatinization treatment by heating.
(6) The composition as described in (1) above, wherein in step (b), the starch removal treatment is carried out by physical destruction treatment.
(7) The composition as described in (6) above, wherein the physical destruction treatment is performed by a homogenizer.
(8) The composition as described in (1) above, wherein in the step (b), the starch-removed fraction is further subjected to a compaction peeling treatment.
(9) The composition according to (1) above, wherein the raw material used for the starch removal treatment is rice bran, wheat germ or barley malt.
(10) The composition according to (1) above, wherein the raw material used for the starch removal treatment is defatted rice bran.

(11) The composition according to (8) above, wherein the raw material used for the starch removal treatment is wheat bran or barley koji.
(12) The composition according to (8) above, wherein the starch-removed fraction is beer-added koji.
(13) The composition according to any one of (1) to (12) above, wherein the enzyme having hemicellulase activity is xylanase.
(14) The composition according to any one of (1) to (13) above, wherein in step (c), a protease is further used in combination.
(15) The composition according to any one of (1) to (14) above, further comprising a degreasing treatment after step (c).
(16) The insoluble fraction of step (d) substantially passes through a 5-25 mesh ASTM (American Society for Testing and Materials) standard sieve but does not pass through a 500 mesh ASTM standard sieve. The composition according to any one of (1) to (15) above, which comprises:
(17) The composition as described in (16) above, wherein the insoluble fraction in step (d) comprises a fraction having a particle size that does not substantially pass through a 200 mesh ASTM standard sieve.
(18) The composition according to any one of (1) to (17) above, wherein the protein content is 20% by weight or less and the dietary fiber content is 55% by weight or more.
(19) The composition according to any one of (1) to (18), wherein the insoluble dietary fiber is partially or completely removed from the aleurone layer.
(20) A composition for sustaining satiety containing insoluble dietary fiber derived from the seeds of a cereal plant having the following characteristics: (i) substantially 5-25 mesh ASTM (American Society for Testing and Materials) standard sieve Consisting of a fraction of particle size that passes through, but does not pass through a 500 mesh ASTM standard sieve; (ii) has a protein content of 20% by weight or less and a dietary fiber content of 55% by weight or more; and (iii) insoluble The aleurone layer of dietary fiber has been partially or completely removed.

(21) The composition according to (20) above, comprising a fraction that does not substantially pass through a 200-mesh ASTM standard sieve.
(22) A diet food or drink containing the composition according to any one of (1) to (21) above.
(23) A satiety-satisfying agent comprising the composition according to any one of (1) to (21) as an active ingredient.
(24) Use of the composition according to any one of the above (1) to (21) for producing a diet food or drink or a satiety maintaining agent.

ADVANTAGE OF THE INVENTION According to this invention, the novel composition (namely, composition for a feeling of fullness) which can maintain the feeling of fullness after ingestion is provided.
The composition for sustaining satiety of the present invention is rich in insoluble dietary fiber derived from the seeds of cereal plants, preferably gramineous plants, and has the advantage of high safety without side effects. Useful as a long-acting agent.

FIG. 1 shows a photograph of an aleurone layer portion contained in Preparation Sample 1. FIG. 2 shows a photograph of the aleurone layer portion contained in Preparation Sample 2. FIG. 3 shows a photograph of the aleurone layer part contained in Preparation Sample 3. FIG. 4 shows a photograph of the aleurone layer portion in Preparation Example 3 when amylase is not allowed to act at 65 ° C. and only starch is removed with cold water. FIG. 5 shows a photograph of the aleurone layer portion contained in Preparation Sample 4. FIG. 6 shows a photograph of the aleurone layer portion contained in Preparation Sample 5. FIG. 7 shows a photograph of the aleurone layer part contained in Preparation Sample 6. FIG. 8 shows a photograph of the aleurone layer portion contained in the prepared sample 14. FIG. 9 shows a photograph of the aleurone layer portion contained in the prepared sample 15. FIG. 10 shows the evaluation of hunger degree 3 hours after ingestion of the food with or without the preparation sample 16 added. FIG. 11 shows the abdomen duration after ingestion of the food with or without Preparation Sample 16 added. FIG. 12 shows the evaluation of the abdominal discomfort after ingestion of the food with or without the preparation sample 16 added. FIG. 13 shows a photograph of the aleurone layer portion contained in the prepared sample 21. FIG. 14 shows a photograph of the aleurone layer portion contained in the prepared sample 22. FIG. 15 shows a photograph of the aleurone layer portion contained in the prepared sample 23. FIG. 16 shows the recovery rate when the prepared samples 21 and 22 were re-degraded with an enzyme. FIG. 17 shows the amount of free sugar when the prepared samples 21 and 22 are re-degraded with an enzyme.

The present invention is based on the finding that an insoluble fraction containing insoluble dietary fiber derived from seeds of cereal plants (hereinafter also referred to as “insoluble dietary fiber-containing substance”) has a satiety-sustaining effect.
Hereinafter, the insoluble food-containing substance of the present invention, a production method thereof, a diet food and drink containing the substance, and a satiety maintaining agent will be described.

The insoluble dietary fiber-containing material of the present invention is characterized in that it contains insoluble dietary fiber derived from the seeds of cereal plants, preferably gramineous plants.
Examples of gramineous plants that can be used in the present invention include all plants classified as gramineous in the plant classification table. Specific examples include rice, barley, wheat, rye, awa, hie, corn, and the like, but are not limited thereto. Of these, rice, barley, rye and wheat are preferably used.

  The insoluble dietary fiber-containing substance of the present invention is produced by recovering an insoluble fraction produced by enzymatic treatment of a starch-removed fraction from which a starch or the like is removed using a grain plant, preferably a grass plant seed. can do.

  Specifically, (a) a step of preparing a raw material by grinding seeds of a cereal plant or by collecting a seed outer fraction produced by mashing; (b) removing the starch from the raw material (C) a step of enzymatic treatment of the fraction prepared in step (b) with an enzyme having hemicellulase activity; and (d) a step of recovering an insoluble fraction from the enzyme-treated solution. It can manufacture by the process containing.

  Step (a) is a step of obtaining the raw material of the insoluble dietary fiber-containing material of the present invention from the seeds of a cereal plant by grinding and / or milling. The pulverization can be performed by, for example, a roll mill, a disk mill, a hammer mill or the like. In addition, milling means removing and separating pericarp, seed coat, germ, etc. from the seeds of cereal plants, which can be carried out by a machine having the principle of scraping the surface, such as a rice mill or a stone mill. it can. In the case of performing spermization, the seed outer fraction containing the pericarp, seed coat and germ produced by this treatment can be used as the raw material in step (b). This step may include threshing treatment if the seeds contain husks in addition to grinding and / or milling the seeds of cereal plants. Moreover, the seeds of the cereal plant to be used may be either germinated or ungerminated.

  Step (a) can be omitted when a material that has been subjected to substantially the same treatment is used as a raw material. Non-limiting examples of such materials include bran, which is produced as a secondary product when wheat flour is produced, and rice bran, which is produced as a secondary product after milling. The use of such materials is preferred from the standpoint that most of the starch in the cereal plant seeds has already been removed and that the steps are simplified. When using rice bran as a raw material, it is preferable to use a defatted rice bran produced after degreasing the rice bran. This is because defatted rice bran is a less expensive material than rice bran and is superior in flavor. Moreover, it has an advantage that more insoluble dietary fiber-containing substances can be obtained as compared with the case where equal amounts of rice bran are used.

  Step (b) is a step of removing starch from the raw material prepared in step (a) to prepare a starch-removed fraction. Any method may be used for the starch removal treatment as long as it can remove starch from the raw material. For example, starch decomposition treatment by an enzyme, gelatinization by heating and removal by sieving after gelatinization, physical destruction It can be performed by a starch removal treatment by, for example.

  When the starch removal treatment is carried out by saccharification treatment with an enzyme, it can be carried out by using amylase or glucoamylase without limitation. Examples of amylase and glucoamylase that can be used in the present invention include, but are not limited to, for example, the brand name Orientase (manufactured by HIBI), the brand name Kokugen (manufactured by Daiwa Kasei Co., Ltd.), the brand name Sumiteam (new) NIPPON CHEMICAL INDUSTRY CO., LTD., Commercial name termamil (manufactured by Novozymes), commercial name gluczyme (manufactured by Amano Enzyme), and other commercially available enzyme preparations, Trichoderma spp., Thermomyces spp., Aurelobasidium spp., Streptomyces spp., Examples include amylase and glucoamylase produced by microorganisms such as Aspergillus genus, Clostridium genus, Bacillus genus, Thermotoga genus, Termocus genus, Cardoseram genus, Thermomonospora genus, Humicola genus, Rhizopus genus, Penicillium genus. The enzyme used in the present invention can be in any form such as a purified enzyme, a crude enzyme, and an immobilized enzyme as long as the enzyme activity is maintained. An immobilized enzyme is an enzyme bound to a carrier such as a polymer, polysaccharide, or inorganic substance. The crude enzyme includes, for example, an enzyme-containing extract from an enzyme-containing microorganism and a processed product such as a dried product. The saccharification treatment is generally performed at a temperature of 10 to 90 ° C., preferably 30 to 60 ° C., and a pH of 3 to 10, preferably 5 to 7. However, the amylase or glucoamylase to be used at an optimum temperature or It is preferable to carry out at a value close to the optimum pH. The enzyme treatment time can be 30 minutes to overnight, for example, 3 hours. The addition amount of the enzyme can be set by those skilled in the art according to the amount of the raw material prepared.

  Gelatinization by heating can be performed by utilizing a phenomenon in which starch is suspended in water and heated, whereby starch granules absorb water and gradually expand to eventually disintegrate and dissolve. Thereafter, the gelatinized starch can be removed by solid-liquid separation with a sieve.

  The starch removal process by physical destruction can be performed by a process using, for example, a homogenizer, a high-speed mixer, a homomixer, a homodisterer, or the like. By the starch removal treatment by these physical destructions, not only starch removal but also a part of the aleurone layer can be removed.

  In the case of using a germinated seed as a seed of a cereal plant, the starch removal treatment can be performed by simply heating without adding amylase or glucoamylase externally. In this case, it is because the germinated seed itself produces amylase.

  In the production method of the present invention, a material that has been subjected to a treatment substantially equivalent to steps (a) to (b) may be used in the subsequent steps as a starch removal fraction. As a non-limiting example of such a material, beer-added straw that is barley malt produced when producing beer can be mentioned. The use of such a material is also preferable from the viewpoint that most of the starch has already been removed and that the steps are simplified.

  The starch-removed fraction prepared in step (b) may contain grain skin, germ, other proteins, lipids, etc. in addition to the main components of the aleurone layer, pericarp and seed coat. Plant tissue such as malt aleurone layer, pericarp and seed coat is shown in Barley: Chemistry and Technology p46 Fig. 8 edited by Alexander W. MacGregor et al. In Journal of Cereal Science 36 (2002) 261-284, it is shown that these are commonly present in the grass seeds.

  Step (b) can further include a pressure stripping treatment of the starch removal fraction. Condensation peeling treatment is a treatment that peels a part of the layer by applying a force parallel to the oppressing surface by passing a composition composed of multiple layers through an appropriate gap in a roll mill with slightly different rotation speeds of the two rolls. Point to. Any crushing machine having a structure that applies a compressive force to the material to be treated can be used for the crushing peeling treatment. For example, in addition to the roll mill having a different rotation speed, a mortar-shaped crushing processing apparatus in which the gap and the rotation speed are controlled can be used for the pressure-removal process. It is particularly desirable to use a roll mill. In this case, the gap between the rolls is 0.15 to 0.01 mm, preferably 0.08 to 0.02 mm, and a size is selected so that the multilayered material to be peeled can be efficiently subjected to physical crushing. As a result, the aleurone layer contained in the starch-removed fraction is exposed, and the removal process of the aleurone layer by the hemicellulase enzyme treatment in step (c) can be made more efficient. The presence / absence of the pressing and peeling treatment can be appropriately selected by those skilled in the art according to the raw material used and the type and state of the starch-removed fraction. For example, when using wheat bran, barley koji koji, and beer-mixed koji as the raw material and starch-removing fraction, the pressure stripping treatment is performed, and when rice koji, defatted rice koji, wheat malt, and barley malt are used. The flat peeling treatment can be omitted. In addition, there is also a suggestion that such a crushing exfoliation treatment can be omitted when germinating seeds are used as cereal plant seeds.

  Step (b) can further comprise a sieving treatment in the presence of water of the starch removal fraction. This sieving treatment removes a large amount of non-target substances contained in the husk and other starch-removed fractions, and a fraction mainly composed of an aleurone layer, pericarp and seed coat (hereinafter also referred to as pericarp / seed coat fraction). ) For rough fractionation. This sieving process can be performed, for example, by passing the starch-removed fraction through a mesh having an appropriate sieve size and removing the fraction remaining on the mesh. The mesh sieve size used in this sieving process may vary depending on the raw material used and the starch removal fraction, but is typically ASTM (American Society for Testing and Materials) standard. Use 5-25 mesh of sieve, eg 12 mesh, 16 mesh, 20 mesh. This treatment is preferable from the viewpoint of handling of subsequent operations because a large amount of non-target substance can be removed.

  In the step (b), it is preferable to use the above-described pressing and peeling treatment and sieving treatment in combination. In this case, this combination of treatments is preferably repeated 2 to 5 times. This is because a fraction mainly composed of an aleurone layer, pericarp and seed coat can be obtained more efficiently.

  As described above, it is possible to determine the presence or absence of a pretreatment such as a crushing exfoliation treatment according to the type of cereal plant seed to be used, its germination state, the type of raw material, and the like. That is, the steps (a) to (b) can be defined as pretreatment steps for more efficiently decomposing the aleurone layer by the hemicellulase enzyme treatment of step (c). Therefore, regarding the starch-removed fraction to be used in step (c), before step (c), confirm whether the pretreatment by steps (a) to (b) is optimal, and if necessary, It may be preferable to add or repeat a pretreatment such as a stripping treatment. At that time, for example, it is possible to determine whether the pretreatment is optimally performed by microscopic observation of the tissue in the starch removal treatment fraction prepared in steps (a) to (b) or analysis using the iodine starch reaction. Conceivable.

  Step (c) is a step of partially or completely removing the aleurone layer contained in the starch-removed fraction or the pericarp / seed coat fraction prepared in step (b) as described above. This step includes enzymatic treatment of the starch removal fraction or the peel / seed coat fraction with an enzyme having hemicellulase enzyme activity. Examples of enzymes having hemicellulase enzyme activity that can be used in this step include, but are not limited to, β-glucosidase, cellulase, xylosidase, xylanase, mannosidase, mannanase, arabinosidase, arabanase, pectinase, A glucanase etc. can be mentioned. It is particularly preferable to use a xylan-degrading enzyme containing xylanase. Specifically, hemicellulase that can be used in the present invention is not limited to this, for example, trade name Cellulosin (manufactured by HIBI), trade name Multifect 720 (manufactured by Gencore Kyowa Co., Ltd.), commodity Commercial enzyme preparations such as name Sumi team (manufactured by Shin Nippon Chemical Industry Co., Ltd.), brand name pentopan (manufactured by Novozymes), brand name hemicellulase "Amano" 90 (manufactured by Amano Enzyme), Trichoderma genus, Thermomyces genus, Oreo List of xylanases produced by microorganisms such as Basidium, Streptomyces, Aspergillus, Clostridium, Bacillus, Thermotoga, Termoiscus, Cardocera, Thermomonospora, Humicola, Rhizopus, Penicillium be able to. The enzyme used in the present invention can be in any form such as a purified enzyme, a crude enzyme, and an immobilized enzyme as long as the enzyme activity is maintained. An immobilized enzyme is an enzyme bound to a carrier such as a polymer, polysaccharide, or inorganic substance. The crude enzyme includes, for example, an enzyme-containing extract from an enzyme-containing microorganism and a processed product such as a dried product. The hemicellulase enzyme treatment is generally carried out at a temperature of 10 to 90 ° C., preferably 30 to 60 ° C., and a pH of 3 to 10, preferably 5 to 7, but the optimum temperature of the hemicellulase enzyme to be used or It is preferable to carry out at a value close to the optimum pH. The enzyme treatment time can be 30 minutes to overnight. In addition, the addition amount of an enzyme can be set by those skilled in the art according to the amount of the prepared starch-removed fraction or pericarp / seed coat fraction. After the hemicellulase enzyme treatment, it is preferable to visually confirm that the aleurone layer in the starch-removed fraction or the pericarp / seed coat fraction is partially or completely removed with a fluorescence microscope or an electron microscope. An analysis method using a fluorescence microscope can be confirmed by a method common to those skilled in the art as described in “PLANT MICROTECHNIQUE AND MICROSCOPY”, STEVEN E. RUZEN, Chapter 7 (p87 to 119). Moreover, it can confirm by the method generally used by those skilled in the art also as the analysis method in an electron microscope.

  In the enzyme treatment in step (c), it is preferable to use a protease treatment in combination. As a result, the amount of the enzyme having hemicellulase enzyme activity used for decomposing the aleurone layer can be reduced to 1/5 to 1/10 times the amount of using the enzyme alone. It is advantageous.

  Step (c) preferably includes a degreasing treatment after the hemicellulase treatment. Thereby, the taste quality and quality of the insoluble dietary fiber-containing substance of the present invention can be improved. The method of degreasing is not particularly limited, but can be performed by degreasing using, for example, ethanol, acetone, hexane, or the like. These techniques are obvious to those skilled in the art. In addition, when using defatted rice bran as a raw material used for the starch removal treatment, it should be noted that the above advantages can be obtained without performing the degreasing treatment.

  Step (d) is a step of collecting the insoluble fraction in the enzyme treatment solution of step (c). Preferably, an insoluble fraction that passes through a 5-25 mesh ASTM standard sieve but does not pass through a 500 mesh ASTM standard sieve is recovered in this step.

  Specifically, this sieving process can be performed by a two-stage method. That is, the hemicellulase treatment solution is sieved with a first mesh, and the fraction that has passed through the sieve is sieved with a second mesh that is finer than the first mesh, and the insoluble matter remaining on the sieve An insoluble fraction rich in dietary fiber (ie, insoluble dietary fiber-containing material) is recovered. The size of the meshes of the first mesh and the second mesh used in the sieving process is not particularly limited as long as it is a combination that can collect a fraction having the above particle size. For example, select 5-25 mesh of ASTM standard sieve as the first mesh, such as 12 mesh, 16 mesh, preferably 20-25 mesh, and select 50-500 mesh of ASTM standard sieve as the second mesh. Can do. By this sieving treatment, a desired fraction in the enzyme treatment solution can be obtained.

  Alternatively, the insoluble fraction recovered in step (d) may be recovered as a fraction that does not pass through a 200 mesh ASTM standard sieve by using a 50-200 mesh ASTM standard sieve as the second mesh. Good.

  In step (b), if the pericarp / seed coat fraction has already been coarsely fractionated, step (d) is not necessarily performed in a two-stage manner, and the sieve using the second mesh is used. What is necessary is just to collect | recover the fractions which remain | survived on the sieve by the division process.

  The insoluble fraction obtained in step (d) is usually dried by lyophilization or the like, and can be used as the insoluble dietary fiber-containing substance of the present invention, preferably without further processing such as fine pulverization.

  The insoluble dietary fiber-containing material of the present invention thus produced is preferably substantially passed through a 5-25 mesh ASTM standard sieve based on the sieving process of step (d), but a 500 mesh ASTM. It consists of a fraction that does not pass through a standard sieve. As used herein, “substantially” is used with the intention that the insoluble dietary fiber-containing material in which a component outside the particle size range is slightly mixed is also included in the scope of the present invention. Therefore, the insoluble dietary fiber-containing substance of the present invention consisting of a fraction that substantially passes through a 5-25 mesh ASTM standard sieve but does not pass through a 500 mesh ASTM standard sieve has a fraction of the above particle size at least 90% or more. , Preferably 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, most preferably 100%.

  In addition, the insoluble dietary fiber-containing substance of the present invention preferably has the properties that the protein content is less than 20% by weight and the insoluble dietary fiber content is 55% by weight or more. The protein content here is obtained from a numerical value obtained by multiplying the nitrogen amount obtained by the Kjeldahl method by 6.25 as a protein conversion coefficient. The dietary fiber content is determined based on the AOAC method.

  The insoluble dietary fiber contained in the insoluble dietary fiber-containing material of the present invention has the aleurone layer partially or completely removed. Here, “partially” means that 70% or more, preferably 80% or more, more preferably 90% or more, more preferably 95% or more, and most preferably 99% or more of the aleurone layer of insoluble dietary fiber is removed. It means being done. It is preferable that the aleurone layer of the insoluble dietary fiber is completely removed.

  The fact that the aleurone layer of the insoluble dietary fiber is partially or completely removed is confirmed by visual confirmation with a fluorescence microscope or an electron microscope as described above, for example, the insoluble dietary fiber-containing material of the present invention Can be confirmed by subjecting to a hemicellulase enzyme treatment and evaluating the recovery after the treatment. Since the insoluble dietary fiber-containing substance of the present invention is a fraction from which most of the aleurone layer has been removed as described above, the recovery rate after the treatment is very high even if it is subjected to a second hemicellulase enzyme treatment. is there. Specifically, the insoluble dietary fiber-containing substance of the present invention is obtained by re-treating hemicellulase overnight with a 4.0% hemicellulase preparation (Sumiteam NX manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 50 ° C.). When subjected to enzyme treatment, it can be characterized by having a recovery of at least about 70% or more, preferably at least about 80% or more. As used herein, “recovery rate” means that the weight of the obtained insoluble dietary fiber-containing substance is 100%, and this is again subjected to enzyme treatment (4.0% hemicellulase, pH 4.5, 50 ° C., overnight). And the relative value of the weight of the substance obtained by lyophilization.

  Other characteristics of the insoluble dietary fiber-containing substance of the present invention include swelling ability. Here, the swelling ability refers to the property that water molecules are taken into the insoluble dietary fiber-containing substance (between plant tissues) and the particles expand and become large. The insoluble dietary fiber-containing material of the present invention has a swelling ability. For example, a certain amount of insoluble dietary fiber-containing material is weighed into a graduated cylinder, and a certain amount of water is added to the graduated cylinder for a certain period of time. Can be confirmed by quantitative determination. The swelling ability of the insoluble dietary fiber-containing material of the present invention can be characterized by swelling to a volume of 20 ml to 35 ml per gram.

Hereinafter, the production of the insoluble dietary fiber-containing material of the present invention in the case where beer-added koji is used as the starch-removing fraction and wheat bran or rice koji is used as the raw material will be specifically described.
As described above, the production of the insoluble dietary fiber-containing material of the present invention is a barley malt produced when beer is produced, beer-added rice bran, bran produced secondary when producing flour, and secondary after rice polishing. It is economically preferable to use rice bran that is produced automatically.

  In the case of using beer-added koji for the production of the insoluble dietary fiber-containing material of the present invention, as an example of a specific method for obtaining the insoluble dietary fiber-containing material of the present invention, the method described in Japanese Patent Publication No. 4-31666 is used. Can be used. That is, the wet beer preparation koji is pressed and pulverized, and the obtained pressed and pulverized product is screened in the presence of water to obtain a fraction containing a large amount of pericarp / seed coat and aleurone layer. It may be prepared and treated with hemicellulase.

  More specifically, in order to remove the barley peculiar to barley, the wet beer-mixed koji is subjected to a crushing peeling process. Any crusher having a structure that gives a compressive force to the raw material to be treated can be used for the pressure peeling treatment of the beer brewer's koji, but the use of a roll mill is particularly desirable. The gap between the rolls is 0.15 to 0.01 mm, preferably 0.08 to 0.02 mm, but the size is selected so that the multilayer material to be peeled can be efficiently physically crushed. It is desirable to adjust the water content in the beer masher to 65% or more when the beer brewer is pressed and peeled. Next, the obtained pressed and peeled product is sieved in the presence of water. By this sieving treatment, the husk fraction remains on the sieve, and the pericarp / seed coat fraction rich in the alloy layer passes through the sieve. The size of the sieve mesh is 5 to 20 mesh, preferably 16 to 20 mesh of ASTM standard sieve. By the above operation, a fraction containing a large amount of barley peculiar to barley can be removed. Further, in order to efficiently obtain a fruit skin / seed coat fraction, sieving is further performed using a finer sieve than the previous sieve. At that time, the pericarp / seed coat fraction remains on the sieve, and the pericarp / seed coat fraction can be prepared. The size of the latter sieve mesh is 50 to 200 mesh of ASTM standard sieve. It is preferable to repeat the above-described pressing and peeling treatment and the sieving treatment 2 to 5 times. The pericarp / seed coat fraction obtained as described above is subjected to hemicellulase treatment, and sieved again with 50 to 200 mesh of an ASTM standard sieve to remove degradation products by hemicellulase and remain on the sieve. The insoluble fraction is the target insoluble dietary fiber-containing material. The insoluble dietary fiber-containing material thus obtained is usually used after being dried. The drying method is not particularly limited, and specific examples include a freeze-drying method.

  When using wheat bran for the production of the insoluble dietary fiber-containing material of the present invention, in order to remove the starch, the starch is decomposed by suspending the dried wheat bran in a solution containing amylase or glucoamylase. Apply the process to be. After the amylase reaction is performed for a long time (for example, 30 minutes to overnight), for example, a pressing and peeling treatment with a roll mill is performed in order to increase the efficiency of the hemicellulase enzyme treatment. The gap between the rolls is 0.15 to 0.01 mm, preferably 0.08 to 0.02 mm, and the size is selected so that the multilayer material to be peeled can be efficiently physically crushed. Next, the sieve mesh is sieved with 50 to 200 mesh, preferably 200 mesh, of an ASTM standard sieve to remove amylase degradation products, and the pericarp / seed coat fraction remaining on the sieve is collected. Next, after suspending this fraction in a solution containing hemicellulase and reacting for a long time (for example, 30 minutes to overnight), similarly, the sieve mesh is ASTM standard sieve 50-200 mesh, preferably 200 mesh. The insoluble dietary fiber-containing substance of the present invention can be obtained by sieving, removing the degradation product due to hemicellulase, and collecting the insoluble fraction remaining on the sieve. The insoluble dietary fiber-containing material thus obtained is usually used after being dried. The drying method is not particularly limited, and specific examples include a freeze-drying method.

  When using rice bran for the production of the insoluble dietary fiber-containing material of the present invention, in order to remove the starch, first, the starch is decomposed by suspending the dried rice bran in a solution containing amylase or glucoamylase. Apply. After the amylase reaction for a long time (for example, 30 minutes to overnight), the sieve mesh is sieved with 50 to 200 mesh, preferably 200 mesh, of an ASTM standard sieve to remove amylase degradation products and Collect the pericarp and seed coat fraction remaining in Next, after suspending this fraction in a solution containing hemicellulase and reacting for a long time (for example, 30 minutes to overnight), similarly, the sieve mesh is ASTM standard sieve 50-200 mesh, preferably 200 mesh. The insoluble dietary fiber-containing substance of the present invention can be obtained by sieving, removing the degradation product due to hemicellulase, and collecting the fraction remaining on the sieve. The insoluble food-containing substance thus obtained is usually used after being dried. The drying method is not particularly limited, and examples thereof include a freeze drying method.

  As mentioned above, although the insoluble dietary fiber containing substance of this invention and its manufacturing method were demonstrated, the manufacturing method of the insoluble dietary fiber containing substance of this invention is not limited to the said method. That is, as long as the insoluble dietary fiber-containing substance having the following characteristics (i) to (iii) can be produced from the seeds of cereal plants, other production methods may be adopted: (i) Consisting essentially of a fraction of a particle size that passes through a 5-25 mesh ASTM standard sieve but does not pass through a 500 mesh ASTM standard sieve; (ii) has a protein content of 20 wt% or less and a dietary fiber content 55% or more; and (iii) the aleurone layer of insoluble dietary fiber is partially or completely removed.

  As an example of an alternative method for producing the insoluble dietary fiber-containing substance of the present invention having the above characteristics, the following steps: (a1) The seed outer fraction produced by grinding or scouring the seeds of a cereal plant (B1) a step of subjecting the raw material to a starch removal treatment including physical destruction; (c1) a 5-25 mesh ASTM standard from the fraction prepared in step (b1); And a method comprising collecting an insoluble fraction that passes through a sieve but does not pass through a 500 mesh ASTM standard sieve.

  This method can be performed in the same manner as the above method except that it is subjected to starch removal treatment including physical destruction and does not include an enzyme treatment step with an enzyme having hemicellulase activity.

  In this method, the starch removal process by physical destruction can be performed by a process using, for example, a homogenizer, a high-speed mixer, a homomixer, a homogenizer, or the like. Thereby, the aleurone layer can be removed together with the starch removal treatment.

  The starch removal treatment is preferably performed a plurality of times, for example, 2 to 5 times in combination with the sieving treatment described in step (b) of the method. Thereby, a starch removal process and the removal of the alloy layer accompanying this can be performed more efficiently.

  The starch removal treatment may be used in combination with starch removal treatment other than physical destruction, such as starch decomposition treatment with an enzyme, gelatinization by heating, and removal by sieving after gelatinization.

  The insoluble dietary fiber-containing material of the present invention produced as described above is characterized in that it is ingested and administered for the purpose of maintaining a feeling of fullness after ingestion. As used herein, the effect of sustaining satiety is meant to include enhancing satisfaction and reducing hunger after ingestion, and increasing the duration from ingestion to hunger. For this purpose, it is desirable to take 1 to 20 g of the insoluble dietary fiber-containing substance of the present invention per serving. The time of ingestion is not particularly limited, and may be any time before meal, between meals or after meal, or may be taken separately from regular meal.

  The insoluble dietary fiber-containing substance of the present invention can also be provided as a diet food or drink with a long stomach by blending in the food or drink. The food form is not particularly limited, and examples thereof include natural products and foods and drinks including processed products thereof. Moreover, although the compounding quantity changes according to the form of food / beverage products, about 1-99g, Preferably about 1-20g can be mix | blended with the insoluble dietary fiber containing substance of this invention with respect to 100g.

  Examples of foods and drinks include, but are not limited to, functional foods such as tablets, powders, granules, capsules, and jelly, and cereal processing such as breads, confectionery, cookies, and biscuits. Products, milk products such as milk, yogurt and ice cream, carbonated drinks, soft drinks, fruit juice drinks, drinks such as medicinal drinks, prepared foods and processed foods.

  The insoluble dietary fiber-containing substance of the present invention can be formulated as a satiety-sustaining agent by blending as an active ingredient in a pharmaceutical product. The administration form of the preparation is not particularly limited, and examples of the administration route include oral administration and enteral administration. In the case of oral administration or enteral administration, the insoluble dietary fiber-containing substance of the present invention may be administered as it is, but together with pharmaceutically acceptable excipients, solutions, suspensions, powders, granules, tablets, capsules You may administer with forms, such as an agent.

  Examples of pharmaceutically acceptable excipients include, but are not limited to, sugars such as lactose, sucrose, and glucose, inorganic substances such as starch, calcium carbonate, and calcium sulfate, crystalline cellulose, distilled water, and purified water. Commonly used oils such as sesame oil, soybean oil, corn oil, olive oil and cottonseed oil can be exemplified. In addition to excipients, additives such as binders, lubricants, dispersants, suspending agents, emulsifiers, diluents, buffers, antioxidants, and bacterial inhibitors can be used. It is also possible to mix or use together with other pharmaceutical products. The above preparation may be sterilized.

  The object to which the satiety-satisfying agent of the present invention is applied is not particularly limited. Thus, for example, patients suffering from one or more of the following diseases or disorders: obesity, hypertension, hyperglycemia, metabolic syndrome, diabetes, bulimia, hyperlipidemia, arteriosclerosis, coronary artery disease, and healthy individuals, It can be ingested and administered to healthy subjects who are considering the prevention of the above diseases. Further, the subject is not limited to humans, and may be applied to animals other than humans.

  The dose of the satiety-sustaining agent of the present invention varies depending on various factors such as the age, weight, sex and degree of obesity of the subject, but typically the insoluble dietary fiber-containing substance of the present invention is 1 1 g or more, 2 g or more, 3 g or more, 4 g or more, 5 g or more, 6 g or more, 7 g or more, 8 g or more, 9 g or more, 10 g or more, 12 g or more, preferably 14 g or more, 16 g or more, 18 g or more, 20 g or more, It is desirable that the amount be ingested and administered at 30 g or more, and the administration interval is not particularly limited.

  The insoluble dietary fiber-containing substance of the present invention can effectively suppress appetite after ingestion and administration, and is a component obtained by treating with cereal plant seeds with food enzymes, so it is safe. It has high properties and is useful for use in foods and drinks or a satiety maintaining agent.

Dietary fiber generally has high water retention and increases bulk. Since this bulk increase effect is thought to be useful for enhancing the feeling of fullness, foods with a large amount of dietary fiber are considered useful as materials with good abdomen. The insoluble dietary fiber-containing material of the present invention has been demonstrated to significantly reduce hunger and significantly increase belly duration compared to intake of foods containing an equal amount of cellulose as dietary fiber content. Therefore (see Test Example 1 below), it has a satiety-sustaining effect superior to that of general dietary fiber.
The insoluble dietary fiber-containing material of the present invention can also be produced from relatively inexpensive raw materials such as rice bran and wheat bran, and thus is excellent in cost.

  When the food / beverage product or medicine contains the insoluble dietary fiber-containing substance of the present invention, it can be detected by the following method. That is, after first suspending food / beverage products or medicine in water, it is sieved in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve to collect the insoluble fraction. The insoluble dietary fiber-containing substance of the present invention can be detected by staining the insoluble fraction with a staining reagent suitable for staining Nile Blue or other hydrophobic substances and observing with a fluorescence microscope or the like. . At that time, the insoluble dietary fiber-containing substance of the present invention can be distinguished from other insoluble compositions by the feature that the layer to be dyed and the layer not to be dyed overlap. Other reagents suitable for staining include Sudan III, Sudan Black, Oil Red, Fluorol Yellow 088, and the like.

  The following examples further illustrate the present invention. However, the scope of the present invention is not limited by these examples. In the examples, unless otherwise specified,% display is based on weight. For each component value determined by analysis, crude protein was measured using the Kjeldahl method (nitrogen protein conversion factor was 6.25), and crude fat was measured using a Soxhlet extraction method using diethyl ether as an extraction solvent. The dietary fiber content was determined based on the AOAC method.

(Example)
[Preparation Example 1] (Beer preparation 粕 ⇒ roll mill)
Wet beer-mixed beer (moisture: 77.6% by weight) is subjected to a pressure peeling treatment with a roll mill (roll rotation speed: 100 rpm, roll gap: 0.08 mm), and then 16 mesh sieve in water (ASTM standard: sieve mesh) Sieved using 1.18 mm), and the passing fraction was further sieved using a 50 mesh (ASTM standard: sieve opening 0.300 mm) sieve, and the fraction not passing through was collected and used for lyophilization. After that, an insoluble dietary fiber-containing material was obtained. This was designated as Preparation Sample 1. The analysis values are as shown in Table 1 below. Further, FIG. 1 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 1.

[Preparation Example 2] (Beer stocking ⇒ roll mill + hemicellulase)
Wet beer-mixed beer (moisture: 77.6% by weight) is subjected to a pressure peeling treatment with a roll mill (roll rotation speed: 100 rpm, roll gap: 0.08 mm), and then 16 mesh sieve in water (ASTM standard: sieve mesh) The fraction passed through was further sieved using a 50 mesh (ASTM standard: sieve opening 0.300 mm) sieve, and the fraction not passing through was collected. The collected fraction was reacted with hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% in 50 mM acetate buffer (pH 4.5, 50 ° C.) overnight to completely complete the alloy tissue layer of the plant tissue. Then, it was sieved using a 200 mesh (sieving aperture: 0.075 mm) sieve in water, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 2. The analysis values are as shown in Table 1 below. Further, FIG. 2 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 2.

[Preparation Example 3] (Naked malt ⇒ starch decomposition)
Germinated bare barley (bare malt) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 500 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Then, it was added with water and kept at 65 ° C. for 3 hours, and starch was decomposed using amylase contained in the naked malt. After starch decomposition, it is sieved using 12 mesh sieve (ASTM standard: sieve opening 1.68 mm) in water, and the passing fraction is further sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve. The fraction that did not pass through was collected and subjected to lyophilization to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 3. The analysis values are as shown in Table 2 below. Further, FIG. 3 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 3. By the way, when the amylase is not allowed to act at 65 ° C. and only the starch is removed with cold water, the aleurone layer is in a state as shown in FIG.

[Preparation Example 4] (Naked malt ⇒ starch decomposition + roll mill + hemicellulase)
Germinated bare barley (bare malt) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 500 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Then, it was added with water and kept at 65 ° C. for 3 hours, and starch was decomposed using amylase contained in the naked malt. After starch decomposition, it is sieved using 12 mesh sieve (ASTM standard: sieve opening 1.68 mm) in water, and the passing fraction is further sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve. The fraction that did not pass through was collected. Next, the collected fraction is pressed in a wet state with a roll mill (roll rotation speed: 100 rpm, roll gap: 0.08 mm), and then sieved to 200 mesh in water (ASTM standard: sieve opening 0.075 mm). The fraction that does not pass through is collected, and hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.). The aleurone layer of the plant tissue was completely decomposed. Again, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 4. The analysis values are as shown in Table 2 below. Further, FIG. 5 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 4.

[Preparation Example 5] (bare barley ⇒ starch decomposition)
Ungerminated bare barley (barley barley) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 800 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Thereafter, 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) was reacted in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours to decompose starch in the bare barley. After starch decomposition, it is sieved using 12 mesh sieve (ASTM standard: sieve opening 1.68 mm) in water, and the passing fraction is further sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve. The fraction that did not pass through was collected and subjected to lyophilization to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 5. The analysis values are as shown in Table 3 below. Further, FIG. 6 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 5.

[Preparation Example 6] (bare barley ⇒ starch decomposition + roll mill + hemicellulase)
Ungerminated bare barley (barley barley) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 800 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Thereafter, 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) was reacted in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours to decompose starch in the bare barley. After starch decomposition, it is sieved using 12 mesh sieve (ASTM standard: sieve opening 1.68 mm) in water, and the passing fraction is further sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve. The fraction that did not pass through was collected. The collected fraction is wet-dried with a roll mill (roll rotation speed: 100 rpm, roll gap: 0.08 mm) and subjected to pressure-crush peeling treatment, and then using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water. The fraction which did not pass through sieving was collected. Next, the collected fraction was reacted with hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Kagaku Kogyo Co., Ltd.) 1.0% in 50 mM acetate buffer (pH 4.5, 50 ° C.) overnight. The layer was completely decomposed. Again, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 6. The analysis values are as shown in Table 3 below. In addition, FIG. 7 shows a scanning electron micrograph image of the aleurone layer portion contained in Preparation Sample 6.

[Preparation Example 7] (Naked malt ⇒ starch decomposition + roll mill)
Germinated bare barley (bare malt) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 500 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Then, it was added with water and kept at 65 ° C. for 3 hours, and starch was decomposed using amylase contained in the naked malt. After starch decomposition, it is sieved using 12 mesh sieve (ASTM standard: sieve opening 1.68 mm) in water, and the passing fraction is further sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve. The fraction that did not pass through was collected. The collected fraction is wet-dried with a roll mill (roll rotation speed: 100 rpm, roll gap: 0.08 mm) and subjected to pressure-crush peeling treatment, and then using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water. After sieving, the fraction that did not pass was collected and subjected to lyophilization to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 7. The analysis values are as shown in Table 4 below.

[Preparation Example 8] ((Naked malt ⇒ starch decomposition + hemicellulase)
Germinated bare barley (bare malt) was used as a raw material. The husk was removed by a brewing test rice mill TDB2A (use rotation speed 500 rpm) manufactured by Satake Seisakusho, and then pulverized by a disk mill. Then, it was added with water and kept at 65 ° C. for 3 hours, and starch was decomposed using amylase contained in the naked malt. After starch degradation, hemicellulase preparation (Sumiteam NX manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.), and the aleurone layer of the plant tissue is completely removed. After decomposing, it was sieved using 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction that did not pass was collected and lyophilized to obtain a substance containing insoluble dietary fiber. . This was designated as Preparation Sample 8. The analysis values are as shown in Table 4 below.

[Preparation Example 9] (Naked malt ⇒ starch decomposition + roll mill + hemicellulase)
Again, the same treatment as in Preparation Example 4 was performed to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 9. The analysis values are as shown in Table 4 below.

[Preparation Example 10] (rice bran ⇒ starch degradation + hemicellulase)
Made from rice bran. The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. . This was designated as Preparation Sample 10. The analysis values are as shown in Table 5 below.

[Preparation Example 11] (rice bran ⇒ starch degradation + hemicellulase)
Again, the same treatment as in Preparation Example 10 was performed to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 11. The analysis values are as shown in Table 6 below.

[Preparation Example 12] (rice bran ⇒ starch decomposition + hemicellulase ⇒ degreasing treatment)
Made from rice bran. The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction not passing through was collected. The collected fraction was degreased with ethanol and subjected to lyophilization to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 12. The analysis values are as shown in Table 6 below.

[Preparation Example 13] (Fusuma ⇒ starch degradation + hemicellulase)
The raw material was bran. The starch in the bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS, manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. . This was designated as Preparation Sample 13. The analysis values are as shown in Table 7 below.

[Preparation Example 14] (Rice bran ⇒ starch degradation + hemicellulase)
Made from rice bran. The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. . This was designated as Preparation Sample 14. The analysis values are as shown in Table 8 below. Further, FIG. 8 shows a scanning electron micrograph image of the aleurone layer portion contained in the prepared sample 14.

[Preparation Example 15] (Rice removal by rice bran ⇒ homogenization stirring)
A rice stirrer is used as a raw material and is suspended in water. k. After subjecting to ROBOMIX at room temperature (10,000 RPM, approx. 20 minutes), it is sieved with 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and starch is collected by collecting the fraction that does not pass Was removed. This operation was repeated twice to increase the starch removal efficiency. In addition, a sterilization step of heating at 80 ° C. was incorporated during the second operation. Finally, the mixture was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the collected non-passed fraction was subjected to lyophilization to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 15. The analysis values are as shown in Table 8 below. Further, FIG. 9 shows a scanning electron micrograph image of the aleurone layer portion contained in the prepared sample 15.

[Preparation Example 16] (defatted rice bran ⇒ starch decomposition + hemicellulase)
The raw material was defatted rice bran (manufactured by Tsukino Food Industry Co., Ltd.). The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. . This was designated as Preparation Sample 16. The analysis values are as shown in Table 8 below.

[Preparation Example 17] (rice bran ⇒ starch decomposition + hemicellulase ⇒ fraction not passing through 200 mesh)
Made from rice bran. The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 17. The analysis values are as shown in Table 9 below.

[Preparation Example 18] (rice bran ⇒ starch degradation + hemicellulase ⇒ 200-500 mesh fraction)
Made from rice bran. The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it is sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the passing fraction is recovered and further filtered to 500 mesh (ASTM standard: sieve opening 0.025 mm). ) Sieving using a sieve, the fraction not passing through was collected and lyophilized to obtain a substance containing insoluble dietary fiber. This was designated as Preparation Sample 18. The analysis values are as shown in Table 9 below.

[Preparation Example 19] (rice bran ⇒ starch degradation + hemicellulase ⇒ fraction not passing through 200 mesh)
The raw material was defatted rice bran (manufactured by Tsukino Food Industry Co., Ltd.). The starch in rice bran was decomposed by reacting 2.0% of an amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 50 mM acetate buffer (pH 4.5, 65 ° C.) for 3 hours. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% is reacted overnight in 50 mM acetate buffer (pH 4.5, 50 ° C.) to completely decompose the alloy tissue layer of the plant tissue. After that, it was sieved again in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and lyophilized to obtain an insoluble dietary fiber-containing substance. . This was designated as Preparation Sample 19. The analysis values are as shown in Table 10 below.

[Preparation Example 20] (Preparation Example 19⇒Fine Grinding⇒500 mesh passing fraction)
The insoluble dietary fiber-containing substance obtained in Preparation Example 19 was finely pulverized (processed by Micro Foods Japan Co., Ltd.), suspended in water, and sieved in water with 500 mesh (ASTM standard: sieve opening 0.075 mm). After sieving, the fraction passing through was collected and lyophilized to obtain a substance containing insoluble dietary fiber. This was designated as Preparation Sample 20. The analysis values are as shown in Table 10 below.

[Test Example 1]
The test food containing the insoluble dietary fiber-containing substance prepared according to Preparation Example 16 was evaluated by feeding. This study was a randomized, single-blind, parallel group controlled study with placebo food as a control.

  Specifically, the test food intake period was 4 days, and the subjects took the test food and the placebo food alternately every day. For the subjects, a group starting with the test food and a group starting with the placebo food were set, and after the test food was ingested, the subject filled in a questionnaire and evaluated. In addition, snacking was prohibited for about one week before the test food intake period and on the test food intake day. Table 11 shows the composition of the test food and placebo food.

  Subjects were ingested with the test food and evaluated for hunger after 3 hours. Evaluation of feeling of hunger was carried out with a score of 5 levels (1. Not at all hungry to 5. Very hungry). The average score evaluation for each test food is shown in the graph of FIG. As is clear from FIG. 10, compared to the placebo food, the hunger degree at 3 hours after ingestion was significantly reduced in the test food.

  Furthermore, the test subject described the time until he / she felt hungry after taking the test food in the questionnaire, and this time was defined as the duration of the stomach. FIG. 11 is a graph showing the average abdominal duration of each test food. As shown in FIG. 11, compared to the placebo food, the test food significantly increased the belly time.

In addition, if a feeling of discomfort in the abdomen was felt after taking the test food, it was indicated in the questionnaire to that effect. The results are shown in FIG.
As shown in FIG. 12, the proportion of subjects who felt abdominal discomfort after ingesting the test food was almost the same as when the placebo food was ingested.

  From the above, it was shown that appetite after ingestion of the food can be significantly suppressed by containing the insoluble dietary fiber-containing substance in the food, and that there is almost no influence on the intestinal environment by the insoluble dietary fiber-containing substance. .

[Preparation Example 21] (defatted rice bran ⇒ starch decomposition + hemicellulase)
An insoluble dietary fiber-containing substance was prepared in the same manner as in Preparation Example 16 using defatted rice bran as a raw material. That is, amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) 1.0% was prepared by adding lactic acid to adjust the pH (pH 4.5, 65 ° C.) and reacting for 3 hours to decompose starch in rice bran. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, with a hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) 0.5%, adjust the pH by adding lactic acid (pH 4.5, 50 ° C.), and let it react overnight. After decomposing, it was sieved in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and subjected to lyophilization to obtain a substance containing insoluble dietary fiber. This was designated as Preparation Sample 21. The analysis values are as shown in Table 12 below.
Further, FIG. 13 shows a scanning electron micrograph image of the aleurone layer portion contained in the prepared sample 21.

[Preparation Example 22] (Defatted rice bran ⇒ starch decomposition + hemicellulase, enzyme amount reduced from Preparation Example 21)
Using defatted rice bran as a raw material, amylase preparation (Sumiteam AS manufactured by Shin Nippon Chemical Industry Co., Ltd.) 0.5%, adjusting the pH by adding lactic acid (pH 4.5, 65 ° C), reacting for 3 hours, starch in rice bran Was disassembled. After starch decomposition, it was sieved using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve in water, and the fraction not passing through was collected. Next, with 0.2% hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.), adjust the pH by adding lactic acid (pH 4.5, 50 ° C), and let it react overnight. After decomposing, it was sieved in water using a 200 mesh (ASTM standard: sieve opening 0.075 mm) sieve, and the fraction that did not pass was collected and subjected to lyophilization to obtain a substance containing insoluble dietary fiber.

  This was designated as Preparation Sample 22. The analysis values are as shown in Table 12 below. Further, FIG. 14 shows a scanning electron micrograph image of the aleurone layer portion contained in the prepared sample 22.

[Preparation Example 23] (Re-hemicellulase treatment of the prepared sample)
Again, prepared sample 22 was hemicellulase preparation (Sumiteam NX made by Shin Nippon Chemical Industry Co., Ltd.) 1.0%, adjusted to pH by adding lactic acid (pH 4.5, 50 ° C), reacted overnight, and again 200 mesh in water (ASTM standard: sieve opening 0.075 mm) The mixture was sieved using a sieve, and the fraction that did not pass was collected and freeze-dried to obtain an insoluble dietary fiber-containing substance. This was designated as Preparation Sample 23. The analysis values of the prepared sample 23 are as shown in Table 13 below. Further, FIG. 15 shows a scanning electron micrograph image of the aleurone layer portion contained in the prepared sample 23.

[Test Example 2]
Prepared samples 21 and 22 were again reacted overnight with hemicellulase preparation (Sumiteam NX, manufactured by Shin Nippon Chemical Industry Co., Ltd.) in 4.0% in 50 mM acetate buffer (pH 4.5, 50 ° C.), and again 200 mesh in water. (ASTM standard: sieve opening 0.075 mm) After sieving using a sieve, the fraction not passing through was collected and subjected to freeze-drying, and then the recovery rate was calculated. In addition, the amount of arabinose and xylose released as the main sugars constituting the aleurone layer during hemicellulase treatment was also investigated.

[Results of Test Example 2]
The recovery rate when the prepared sample 21 was re-decomposed with hemicellulase was 78%, and the recovery rate when the prepared sample 22 was similarly decomposed was 69% (FIG. 16). Further, as a result of investigating the amounts of free arabinose and xylose when each sample was decomposed, it was found that both of the cases where the prepared sample 22 was decomposed had more free arabinose and xylose (FIG. 17). Arabinose and xylose are main sugars constituting the aleurone layer, and the fact that these sugars are liberated by the hemicellulase treatment can be inferred that the aleurone layer is decomposed by the hemicellulase treatment.

  A substance containing insoluble dietary fiber derived from the seeds of a cereal plant of the present invention, preferably a grass family, is provided as a composition for sustaining satiety. The insoluble dietary fiber-containing substance of the present invention has no side effects and can be used in various foods or drinks or as an active ingredient of a medicine.

Claims (24)

The following steps:
(a) preparing the raw material material by grinding the seeds of the cereal plant or by recovering the outer seed fraction produced by scouring;
(b) preparing a starch-removed fraction by subjecting the raw material to a starch-removing treatment;
(c) enzymatically treating the fraction prepared in step (b) with an enzyme having hemicellulase activity; and
(d) A composition for sustaining satiety, comprising insoluble dietary fiber derived from seeds of a cereal plant produced by a treatment comprising a step of recovering an insoluble fraction from the enzyme treatment solution.   2. The composition according to claim 1, wherein the cereal plant is a gramineous plant.   3. The composition according to claim 2, wherein the gramineous plant is rice, barley, rye or wheat.   2. The composition according to claim 1, wherein in the step (b), the starch removal treatment is performed by an enzyme treatment using amylase or glucoamylase.   2. The composition according to claim 1, wherein in step (b), the starch removal treatment is performed by gelatinization treatment by heating.   2. The composition according to claim 1, wherein in step (b), the starch removal treatment is carried out by a physical destruction treatment.   7. The composition according to claim 6, wherein the physical destruction treatment is performed by a homogenizer.   2. The composition according to claim 1, wherein in the step (b), the starch-removed fraction is further subjected to a pressing and peeling treatment.   2. The composition according to claim 1, wherein the raw material used for the starch removal treatment is rice bran, wheat germ or barley malt.   2. The composition according to claim 1, wherein the raw material used for the starch removal treatment is defatted rice bran.   9. The composition according to claim 8, wherein the raw material used for the starch removal treatment is wheat bran or barley koji.   9. The composition according to claim 8, wherein the starch-removed fraction is a beer-added koji.   The composition according to any one of claims 1 to 12, wherein the enzyme having hemicellulase activity is xylanase.   14. The composition according to any one of claims 1 to 13, wherein a protease is further used in combination in step (c).   The composition according to any one of claims 1 to 14, further comprising a degreasing treatment after step (c).   The insoluble fraction of step (d) consists essentially of a fraction of particle size that passes through a 5-25 mesh ASTM (American Society for Testing and Materials) standard sieve but does not pass through a 500 mesh ASTM standard sieve. 16. The composition according to any one of claims 1 to 15, wherein:   17. The composition of claim 16, wherein the insoluble fraction of step (d) comprises a fraction of a particle size that does not substantially pass through a 200 mesh ASTM standard sieve.   18. The composition according to any one of claims 1 to 17, wherein the protein content is 20% by weight or less and the dietary fiber content is 55% by weight or more.   The composition according to any one of claims 1 to 18, wherein the insoluble dietary fiber is partially or completely removed from the aleurone layer. A satiety sustaining composition comprising insoluble dietary fiber derived from the seeds of a cereal plant having the following characteristics:
(i) consisting of a fraction of a particle size that substantially passes through a 5-25 mesh ASTM (American Society for Testing and Materials) standard sieve but does not pass through a 500 mesh ASTM standard sieve;
(ii) the protein content is not more than 20% by weight and the dietary fiber content is not less than 55% by weight; and
(iii) The aleurone layer of insoluble dietary fiber is partially or completely removed.   21. The composition of claim 20, comprising a fraction that does not substantially pass through a 200 mesh ASTM standard sieve.   A diet food or drink comprising the composition according to any one of claims 1 to 21.   A satiety sustaining agent comprising the composition according to any one of claims 1 to 21 as an active ingredient.   The use of the composition according to any one of claims 1 to 21 for producing a diet food or drink or a satiety maintaining agent.

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