JP5514390B2 - Life extension agent made from cereals and beans other than rice - Google Patents

Description

  The present invention relates to a life extending agent using cereals and beans other than rice as raw materials.

Individuals, including humans, are anxious to live a long life, usually in a situation where no one can avoid the reality that everyone dies and dies. By the way, it is well known that alcohol, which is said to be the head of a hundred medicines, has a particularly positive effect on the mental aspect, such as making people cheerful and relieving stress. And moderate liquor is thought to make people live longer through relieving stress. Here, in the case of liquor, alcohol (ethyl alcohol) contained in the liquor is an active ingredient for relieving stress.
PCT / JP2005 / 000261

  However, the above-mentioned alcohol itself does not directly have a life extension effect, and the effect is indirect. Also, it has been confirmed that alcohol oxides are toxic or ineffective. Moreover, continuous alcohol consumption tends to be alcoholic, and excessive alcohol consumption can lead to alcoholism, both of which can rather result in a shortened lifespan.

  By the way, the present inventors are carrying out various studies focusing on white rice, which is a staple food and has the highest safety, from the viewpoint of combining plants and animals. In the middle of these studies, it was discovered by chance that the raw material that had been subjected to the predetermined treatment had a significant effect of extending the life, and a patent application has already been filed for the treated product (see Patent Document 1). However, there is a problem that the cost increases when rice is used as a raw material.

  Therefore, as a result of repeated changes from various angles such as raw materials and processing conditions, the present inventors have found that when a predetermined natural material is subjected to a predetermined treatment, an effective component for life extension can be produced. The headline and the following present inventions (1) to (5) are completed.

  The present invention (1) is a life extending agent characterized by comprising as an active ingredient a processed product of raw materials essentially containing cereal or beans other than rice.

  This invention (2) is a life extension agent of the said invention (1) whose said processed material is the water extract or organic-solvent extract of the said raw material.

  This invention (3) is a life extension agent of the said invention (1) whose said processed material is the saccharified product of the said raw material or the water extract of the said raw material, or an organic solvent extract.

  In the present invention (4), the treated product is an alcoholic fermentation of the raw material, the water extract or organic solvent extract of the raw material, or the saccharified product of the raw material or the water extract or organic solvent extract of the raw material. It is a life extension agent of the said invention (1) which is a product or an organic acid fermented product.

  This invention (5) is a life extension agent in any one of said invention (1)-(4) whose cereals or beans except rice are wheat, barley, corn, or soybean.

  Here, the meaning of each term in this specification is explained. For convenience of explanation, the meaning of some terms will be described in the section “Best Mode for Carrying Out the Invention”.

  First, “water-treated product” refers to a processed product of a raw material using water, for example, a water extract of the raw material, a saccharified product in the presence of water (for example, saccharified by adding water to the raw material, Saccharified raw water extract), fermented product in the presence of water (for example, fermented by adding water to raw material, fermented raw water extract, raw water extract) Saccharified and fermented). Moreover, "water" here means an aqueous medium that essentially contains water, and includes, for example, water or a mixture of water and alcohol.

  “Water extract” refers to physical treatment (eg, pressing, heat treatment), chemical treatment (eg, acid or alkali treatment), biological (biochemical) treatment (eg, straw, (Microbe treatment, enzyme treatment) is applied alone or in combination. For example, water added to raw material, raw material treated with acid or alkali, raw material hydrolyzed with enzyme or koji (before or simultaneously with extraction), or these treatments heated or The aspect which is performed under non-heating can be mentioned. As described above, the “water extract” includes an embodiment in which saccharification enzymes and koji are allowed to act, and thus includes a saccharified product. The “saccharified product” is the above-mentioned “saccharified product”. It is a different concept.

  "Organic solvent extract" means performing a physical process (for example, pressing, heat processing) using an organic solvent, for example.

  “Contained as an active ingredient” means containing the component to the extent that it has a life extending effect, and not only when the component constitutes a part of the life extending agent, but the component is also a life extending agent. Including all of the above.

  “Organic acid fermentation” refers to, for example, acetic acid fermentation or lactic acid fermentation.

  “Life extension agent” means an agent that extends the life of a cell, tissue, organ or individual compared to when it is administered or not. Here, the living organisms related to cells, tissues, organs or individuals refer to multicellular organisms (including higher animals such as mammals including humans) and unicellular organisms.

  According to the present invention, when administered to an individual such as a human, the lifespan of the cells is extended, and the lifespan of various cell groups (tissues and organs) composed of the cells is extended. There is an effect that the life is extended. In addition, cell functions, cell activity, cell cycle, youthfulness, normalization of gene functions are brought about with respect to cells constituting an individual, and further, functions of organs, organs, tissues, and body fluids constituting an individual are also improved. Improves or improves, normalizes, restores health, prevents aging, maintains youthfulness or rejuvenates. Furthermore, as a result of these, many diseases are prevented and cured. For example, circulatory system (heart, blood vessel, blood / hematopoietic system), immune system (lymphoid tissue / organ, spleen, thymus), skin, skin appendages, digestive system (lip, tonsils, teeth, esophagus, stomach) , Small intestine, large intestine, liver, biliary system, spleen), respiratory system (nose, pharynx, larynx, trachea, lung), urinary system (kidney, urinary tract), sex breeding system (male genital organ, female genital organ), endocrine system (Pituitary gland, pineal gland, thyroid gland, adrenal gland), brain, nervous system (peripheral nerve, central nervous system), metabolic system (sugar, lipid, uric acid metabolism), balance / hearing apparatus, visual instrument, each mucous system, motor organ (Bone, joints, muscles, tendons, ligaments) function improvement or improvement, normalization, health, anti-aging, youthfulness retention or rejuvenation; life forms or individuals, organs, organs, tissues, body fluids or cells, etc. Prevention, prevention, improvement, treatment of diseases and symptoms in Japan; cardiovascular system (heart, blood vessel, blood / hematopoietic system), Epidemiological system (lymphoid tissue / organ, spleen, thymus), skin, skin appendages, digestive system (lips, tonsils, teeth, esophagus, stomach, small intestine, large intestine, liver, bile duct, spleen), respiratory system ( (Nose, pharynx, larynx, trachea, lungs), urinary system (kidney, urinary tract), sex breeding system (male genital organ, female genital organ), endocrine system (pituitary gland, pineal gland, thyroid gland, adrenal gland), brain, nervous system (Peripheral nerve, central nervous system), metabolic system (sugar, lipid, uric acid metabolism), balance / auditor, visual organ, each mucosal system, motor organs (bone, joint, muscle, tendon, ligament), etc. and various symptoms Prevention, deterioration prevention, improvement, treatment of skin; skin disorders caused by epidermis / dermis, cell disorders, DNA disorders, atopic dermatitis, dry skin symptoms such as dry skin (atopy skin, dry / sozoized skin, aging skin) , Ichthyosis, bulky skin, rough skin, sebum deficiency, xeroderma, dry eczema, progressive Palmokeratosis, erythema, sclerosis / keratinization, cracks, scales, crests, itching, scarring), spots, buckwheat, pruritus, skin rash, roughness, moistness, cracks, redheads, skin aging symptoms (wrinkles, tension, elasticity Reduction, sagging, stains), oily skin, acne, dyschromia, UV-induced skin damage (stains, freckles), photoinflammation, dullness, darkening, physical / chemical skin disorders (cuts, burns, bedsores), water, Skin symptoms caused by the use of soaps, detergents, surfactants, solvents, side effects of pharmaceuticals such as topical skin preparations, biological skin disorders (skin infections), athlete's foot, dermatitis, eczema, wound healing, hair growth, hair restoration Prevention, deterioration prevention, improvement, treatment of hair growth, alopecia; prevention, prevention, improvement, treatment of spots, buckwheat, senile pigment spots; various inflammations, infections, allergies, immune dysfunction, mucosal disorders, cells Disorder, DNA disorder , Tumor, ulcer, hyperlipidemia, obesity, diabetes, arteriosclerosis, gout, blood pressure restoration, improvement (high blood pressure, hypotension), blur, dementia, rhinitis, asthma, various symptoms of the eye, cold, vascular disorder , Internal stiffness, shoulder stiffness, lottery, neuralgia, low back pain, rheumatism, hemorrhoids, recovery from fatigue, prenatal birth cold, stomatitis, throat inflammation, pain, pharyngitis, laryngitis, tonsillitis, periodontitis, alveolar pus effusion, gingivitis , Osteoporosis, nourishing tonic, weak constitution, physical fatigue, physical strength reduction, salt damage prevention, Helicobacter pylori growth inhibition, stress, prevention of side effects caused by drugs, prevention, improvement, treatment; tumor prevention, prevention of deterioration, improvement, treatment, Cancer prevention, deterioration prevention, progression inhibition, metastasis prevention, improvement, treatment; sexual function improvement, improvement, normalization, restoration or prevention of aging, youthfulness retention or rejuvenation can be expected. Furthermore, it is also useful as an organ preservative or cell preservative (used as a medium).

  The “cereal or beans other than rice” according to the present invention is, for example, wheat, barley, corn, rice, wax, buckwheat, rye, etc., excluding rice, and beans are soybeans, peas, beans, red beans, etc. And broad beans. Further, the beans may be in any state such as immature raw beans, fully-ripe raw beans, and dry-processed fully-ripe beans.

  When cereals and beans are extracted with a water extract or an organic solvent, first, when the cereals and beans are ground or pulverized, the surface area becomes large, and thus the extraction efficiency is extremely good. This method may be a general method using a pulverizer or the like. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the grain structure and the bean structure.

  In the water extraction, cereals and beans are added as they are, preferably pulverized or powdered. The cereal may be brown or refined. The amount of water added can be efficiently extracted in an amount 2 to 5 times that of cereals and beans, but may be appropriately selected according to the yield, workability, end use purpose, and the like. After this, the mixture is heated and the extraction is completed when it reaches a boiling state. After completion of the extraction, a clear extract can be obtained by pressing and filtering according to the purpose of use. In addition, you may extract by adding hot water from the beginning.

  Although the active ingredient in the extract has not been elucidated, it has been confirmed that this unknown active ingredient is stable to heat, so that the extraction temperature at the time of water extraction is efficient. Extraction can be carried out sufficiently by placing it at a low temperature for a long time. However, in the case of a low temperature of 40 ° C. or lower, it is preferable to make the pH acidic or alkaline, or to add a preservative. In the case of boiling extraction, the extraction time may be several minutes, but in the case of a medium temperature lower than that, several hours to one day are required. In the case of low temperature, it takes several days to one month depending on the pulverized state of grains and beans. However, even in this case, it is more effective to heat at the end as much as possible.

  The most serious problem in the case of water extraction is the gelatinization phenomenon. If it becomes paste-like, not only extraction efficiency will worsen but it will be extremely difficult in actual work. In order to prevent this, starch may be decomposed by adding amylase to react or acidifying with hydrochloric acid or the like. By using this method, the problem can be solved sufficiently and there is no problem in practical use.

  It is also effective to perform acid extraction or alkali extraction because the active ingredient in the extract is stable to acid and alkali. Furthermore, in the case of water extraction, an enzyme (for example, cellulase) that acts on the grain or bean tissue may be allowed to act. The enzyme here is one or a combination of two or more of amylolytic enzymes (liquefaction enzymes, saccharifying enzymes), proteolytic enzymes, lipolytic enzymes, fiber-degrading enzymes, lignin-degrading enzymes, and pectin-degrading enzymes. In addition, when performing extraction, the timing which acts an enzyme may be either before extraction or simultaneously with extraction.

  Furthermore, it has been found that an extract having this effect can also be extracted by organic solvent extraction. This is extremely effective in elucidating the active ingredient, and in use such as extracting the active ingredient in a concentrated manner or blending with an ingredient that is insoluble in water. In this case, it is preferable to pulverize or powder as much as possible. In addition, it is desirable to use an organic solvent that is safe to administer to the human body such as alcohol.

  In addition, in the case of fresh cereals such as corn (including those that have been heat-sterilized) and green beans (including those that have been heat-treated), the sample is squeezed with a cloth or a press without using water or an organic solvent (squeezed) Juice). Furthermore, the residue after pressing may be extracted with water or an organic solvent.

  Furthermore, you may combine organic acid fermentation, such as alcohol fermentation, lactic acid fermentation, and acetic acid fermentation.

  In addition, depending on the application, sugar or dextrin may be included in the juice or water extract, or it may become an obstacle in its effect. In that case, after dextrin is saccharified with amylase, the sugar may be removed by feeding the sugar to yeast, fractionating the active ingredient with an adsorbent, or extracting with an organic solvent. In any case, an effect can be obtained by performing extraction, and unnecessary components may be removed by various methods depending on applications.

  The life extension agent according to the present invention obtained as described above is applied to an object (cell, tissue, organ or individual) as it is without being separated from the residue or after being squeezed or filtered. Apply. Here, when used as it is, it is preferable to perform sterilization or sterilization. The life extension agent according to the present invention may be used internally or externally, for example, when applied to an individual. Specifically, according to the actual application, according to conventional methods, cosmetics such as creams, facial cleansers, emulsions, lotions, cleansings, packs, soaps, ointments, pasta agents, lotions, tinctures, ligaments, Jelly, aerosol, liquid, jelly, elixir, capsule (soft capsule, hard capsule), granule, pill, suspension, emulsion, powder, fine granule, tablet, syrup, injection It is used in oral dosage forms such as pills, troches and limonades, and in dosage forms such as injections. Furthermore, there may be a prescription added to foods and beverages. In addition, you may add another compounding component and a chemical | medical agent according to a use. The dosage when administered to humans depends on the age, body weight, severity, etc. of the user, but generally 0.0001-90 parts by weight of the extract of the present invention per 100 parts by weight of the product is preferable. Is added in an amount of 0.01 to 60 parts by weight, most preferably 0.1 to 30 parts by weight. The intake of the product of the present invention is 0.0001 to 100 ml per day, preferably 0.001 to 50 ml, most preferably 0.01 to 20 ml per kg body weight.

  In relation to the actual product, for example, an indication that the product is used for extending the life of the product, such as a drug, a cosmetic, a food and drink package, a pamphlet, and an instruction manual. Is preferred. By attaching such a display, it is obvious to the user that the product of the present invention has such an effect. In addition, when it cannot be displayed directly on a product due to restrictions of other laws (pharmaceutical law), for example, a method of indirectly informing the user of the effectiveness of the product in the form of newspaper articles, etc. A method of notifying the user of the effect is also assumed. Therefore, the “indication that the product is used for extending the life” includes not only the indication or suggestion of the extension of the life directly on the product but also the indication or announcement indirectly including these methods. Actions are also included. Moreover, as a life extension effect, it is preferable to play at least about 10% of the results shown in the test examples, more preferably about 50%, and most preferably about 80%.

Production Example 1 (Wheat extract)
10 kg of wheat was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Then, it was squeezed with a squeezing machine to obtain 25 L of this example product and 13 kg of residue.

Example 2 (Barley extract)
10 kg of barley was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Then, it was squeezed with a squeezing machine to obtain 25 L of this example product and 13 kg of residue.

Production Example 3 (corn extract)
10 kg of corn was pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, the product was squeezed with a squeezing machine to obtain 12 L of this example product and 26 kg of residue.

Production Example 4 (Soybean extract)
10 kg of soybeans were pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, the product was squeezed with a squeezing machine to obtain 6 L of this example and 30 kg of residue.

Production Example 5 (Saccharified wheat)
10 kg of wheat was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 26 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Example 6 (Saccharified barley)
10 kg of barley was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 26 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Production Example 7 (Saccharified corn)
10 kg of corn was pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 15 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Production Example 8 (Saccharified soybean)
10 kg of soybeans were pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. Thereafter, solid-liquid separation was performed using a filter to obtain 8 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Production Example 9 (fermented wheat)
10 kg of wheat was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. After cooling, yeast was added and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 26 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Example 10 (fermented barley)
10 kg of barley was pulverized well, and 30 L of warm water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. After cooling, yeast was added and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 26 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Production Example 11 (fermented corn)
10 kg of corn was pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. After cooling, yeast was added and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 15 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Production Example 12 (fermented soybean)
10 kg of soybeans were pulverized well, 30 L of hot water at 60 ° C. and 50 g of liquefied enzyme were added thereto and stirred well. Thereafter, the temperature was gradually raised, boiled and extracted for 5 minutes, and then cooled to 30 ° C. Thereafter, 50 g of amylolytic enzyme was added and left at 55 ° C. for 4 hours. After cooling, yeast was added and fermentation was performed for 15 days. Thereafter, solid-liquid separation was performed using a filter to obtain 8 L of a filtrate. This was heated at 85 ° C. for 30 minutes to obtain this example product.

Test example (cell life extension test)
・ Used cells: Osteoblast (mouse Osteoblast MC3T3-E1)
-Medium used: DMEM
Sample used: Production Example 11
Osteoblasts were seeded in a 6-well microplate at a concentration of 10%, and precultured at 37 ° C. and 5% CO ₂ until the cells adhered. On the next day, it was confirmed that the cells were adhered, and the sample was added to a predetermined concentration (5%) with respect to the medium. The culture was performed at 37 ° C. in the presence of 5% CO ₂ . It was confirmed that the cells became confluent within a few days, and the appearance of the cells after 27 days was visually evaluated. For comparison, the same test was performed when only water was added and when the rice extract already filed by the present inventor (see Patent Document 1) was added. The result is shown in FIG. Here, FIG. 1 (a) is a photograph when only water is added, FIG. 1 (b) is a photograph when Production Example 11 is added, and FIG. 1 (c) is a photograph when rice extract is added. . As is clear from the figure, it was found that when Production Example 11 was added, the state of the cells was significantly better than that of water. In addition, it was also found that the case where Production Example 11 was added was comparable to the case where rice extract was added. Although the results are not specified, similar results were obtained for other production examples.

It is the photograph which showed the result of the test example.

Claims (1)

An agent comprising an alcohol fermented product of saccharified product obtained by extracting corn with water, the osteoblast being in a better state compared to the case where the agent is not applied . Agent for improvement .