JP2019178123A - Glycyrrhiza extract fermented product, whitening agent, anti-aging agent, skin cosmetic and food and drink - Google Patents

Abstract

To provide novel compositions having at least one of an excellent whitening action and an anti-aging action, and having high safety, to provide highly safe whitening agents having excellent whitening action, to provide highly safe anti-aging agents having excellent anti-aging action, to provide highly safe skin cosmetic having at least one of an excellent whitening action and an anti-aging action, and to provide highly safe food and drinks having at least one of an excellent whitening action and an anti-aging action.SOLUTION: Provided are a glycyrrhiza extract fermented product, a whitening agent or an anti-aging agent containing the glycyrrhiza extract fermented product, and a skin cosmetic or a food and drink containing at least one selected from the group consisting of the glycyrrhiza extract fermented product, the whitening agent and the anti-aging agent, the fermented product being a fermented product by microorganisms, the glycyrrhiza being Glychyrrhiza glabra, and the microorganism being Lactobacillus plantarum.SELECTED DRAWING: None

Description

The present invention is fermented by Gurichiruriza glabra (Glychyrrhiza glabra) extract of Lactobacillus plantarum (Lactobacillus plantarum), whitening agents and anti-aging agent containing the same, and to skin cosmetics and food products.

  Conventionally, for the prevention, treatment or improvement of skin pigmentation, stains, buckwheat, etc., a treatment using a whitening agent containing a chemically synthesized product such as hydroquinone as an active ingredient has been performed. However, chemically synthesized products such as hydroquinone may cause side effects such as skin irritation and allergies. Therefore, development of a whitening agent containing a highly safe natural raw material as an active ingredient is desired. Examples of those having an inhibitory effect on tyrosinase activity include, for example, a willow extract (see, for example, Patent Document 1). ing.

  Filaggrin is a structural component of the skin, is involved in the barrier function in the skin, and is considered to have a function of preventing the entry of allergens, toxins and infectious organisms. Reduced function due to filaggrin gene mutation is associated with the risk of developing atopic diseases including atopic dermatitis (eczema, skin inflammation, skin itch, etc.), allergy, asthma, etc. In some cases, it is known to lead to skin diseases such as ichthyosis vulgaris (see Non-Patent Document 1, for example).

  On the other hand, amino acids which are the main components of natural moisturizing factors (NMF) are produced by the degradation of filaggrin derived from keratohyalin granules in the stratum corneum. This filaggrin is expressed as profilagrin in epidermal keratinocytes present in the granule layer immediately below the stratum corneum. After that, it is immediately phosphorylated, accumulated in keratohyalin granules, decomposed to filaggrin through dephosphorylation and hydrolysis, transferred to the stratum corneum, increases the aggregation efficiency of keratin filaments and participates in the internal construction of keratinocytes It is known (for example, refer nonpatent literature 2). In recent years, it has been known that this filaggrin is very important and indispensable for moisture retention of the skin, and that the synthetic power of filaggrin is reduced by conditions such as drying, and the amount of amino acids in the stratum corneum is reduced ( For example, refer nonpatent literature 3).

  Therefore, by promoting the expression of profilagrin in epidermal keratinocytes, it is possible to prevent, treat or ameliorate atopic diseases including atopic dermatitis (eczema, skin inflammation, skin itching, etc.), allergy, asthma, etc. it is conceivable that. Moreover, it is expected that the water environment of the stratum corneum can be essentially improved by promoting the expression of profilagrin and thereby increasing the amount of amino acids in the stratum corneum. Conventionally, as a thing which has a profilaggrin mRNA expression promotion effect, a gaiyo extract (for example, refer patent document 2) etc. are known.

  The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as collagen, elastin, and hyaluronic acid that are outside these cells and support the skin structure. In young skin, the proliferation of fibroblasts is active, and the retention of moisture, flexibility, elasticity, etc. is ensured by maintaining the homeostasis of the interaction of skin tissues such as fibroblasts and extracellular matrix components. Is kept fresh with tension and gloss.

  However, collagen, elastin, and hyaluron, which are the main components of the extracellular matrix, are affected by certain external factors such as UV irradiation, significant drying of the air, excessive skin cleansing, and so on. As acid production decreases, it causes degradation and alteration. As a result, the moisturizing function and elasticity of the skin are lowered and abnormal exfoliation of the keratin occurs, so that the skin loses its tension and gloss, and exhibits aging symptoms such as rough skin and wrinkles. As described above, changes associated with aging of the skin, that is, wrinkles, dullness, changes in texture, decrease in elasticity, and the like involve reduction / denaturation of matrix components such as collagen, elastin, and hyaluronic acid. Therefore, promoting the production of hyaluronic acid and the like is important for preventing, treating or improving skin aging.

  Of the extracellular matrix components described above, hyaluronic acid is a kind of mucopolysaccharide, and has a function of retaining cells by being filled in the interstices between cells. It has a number of functions such as providing lubricity and flexibility, and resistance to external forces such as mechanical failure. If the production of hyaluronic acid can be promoted, it is considered that skin aging symptoms such as rough skin, wrinkles, dullness, texture change, reduced elasticity and reduced moisturizing function can be prevented, treated or improved. Moreover, it is considered that aging of the skin can be prevented, treated or improved by promoting the expression of hyaluronic acid synthase 3 (HAS3) involved in promoting the synthesis of epidermal hyaluronic acid.

  Furthermore, hyaluronic acid is present in cartilage, joint fluid, umbilical cord, ophthalmic vitreous and other connective tissues in addition to skin tissue. Among these, the hyaluronic acid contained in the joint fluid covers the surface of the articular cartilage and is useful for smooth operation of the joint due to the lubricating function of the hyaluronic acid, the cartilage covering / protecting function, and the like. On the other hand, in arthritis such as rheumatoid arthritis, it is known that the concentration of hyaluronic acid in joint fluid is reduced. Therefore, it is considered that by promoting the production of hyaluronic acid, arthritis such as rheumatoid arthritis, osteoarthritis, septic arthritis, gouty arthritis, traumatic arthritis, or osteoarthritis can be prevented or treated. Furthermore, in the healing process of wounds or burns, granulation (tissue) is formed, but it is known that hyaluronic acid significantly increases in the granulation. Therefore, it is considered that healing of wounds or burns can be promoted by promoting production of hyaluronic acid. As what has a hyaluronic acid production promotion effect | action, the extract (for example, refer patent document 3) etc. from Kusunohagashi is known.

  Among the basal layer, spiny layer, granule layer, and stratum corneum constituting the epidermis, in particular, in the granule layer, the cell membrane thickens to form a thickened cell membrane, and the action of transglutaminase-1 Between molecules, glutamyl-lysine crosslinks are formed, and strong keratin protein fibers are formed. Furthermore, ceramide or the like is covalently bonded to a part of the ceramide to form a hydrophobic structure, thereby providing a foundation for the lamellar structure of the intercellular lipid and forming the basis of the keratin barrier function.

  Ceramide is produced by the action of an enzyme such as serine palmitoyltransferase (SPT) known as a rate-limiting enzyme for ceramide synthesis based on serine and palmitoyl-CoA in the process of keratinization of epidermal cells. Ceramide exists specifically as the main component of the keratinocyte lipid covering the outermost layer of the skin, and plays an important role in maintaining the function of the skin itself as a barrier film between the living body and the outside world.

  The stratum corneum structure is compared to brick and mortar, and a strong barrier membrane is formed in such a way that intercellular lipids connect the stratum corneum stacked in about 15 layers. Keratinocytes retain water by containing natural moisturizing factors mainly composed of amino acids in the cells, while stratum corneum lipids are composed of approximately 50% ceramide as a main component, and parents such as cholesterol and fatty acids. It is composed of an amphiphilic lipid, and is characterized by a lamellar structure in which hydrophobic portions and hydrophilic portions are alternately repeated, so-called lamellar structure.

  The decrease in the skin barrier function due to various internal and external factors increases the transepidermal water transpiration, causing skin roughness, desquamation, pruritus, etc., resulting in so-called dry skin. Moreover, the decrease in the barrier function of the skin increases the inflammation of the skin and falls into a vicious circle in which the protective function against various stimuli from the outside world is reduced. In recent studies, a decrease in keratin ceramide component (so-called intercellular lipid) and composition change have been reported in patients with atopic dermatitis known as aging or barrier disorder (see, for example, Non-Patent Document 4). It is widely known that ceramide is important for maintaining and improving the barrier function of the skin. Known methods for improving the barrier function of the skin include a method of supplementing ceramide from the outside (see, for example, Non-Patent Document 5), a method for enhancing the ability to produce ceramide in the skin (for example, see Non-Patent Document 6), and the like. .

  However, there is still a strong demand for a substance that has a more excellent whitening effect or anti-aging effect and is highly safe and can be used as an active ingredient in a whitening agent, an anti-aging agent, a skin cosmetic, or a food and drink. The current situation is that rapid development is required.

JP 2004-083488 A JP 2012-219047 A JP 2003-146837 A

“Nat Genet.”, 2006, Vol. 38, no. 4, p. 441-446 "Fragrance journal extra edition", 2000, Vol. 17, p. 14-19 “Arch. Dermatol. Res.”, 1996, Vol. 288, p. 442-446 J. et al. Dermatol. 1993, Vol. 20, no. 1, p. 1-6 “Fragrance Journal”, 2004, Vol. 32, no. 11, p. 23-32 Br. J. et al. Dermatol. , 2000, Vol. 143, Issue 3, p. 524-531

An object of the present invention is to solve the conventional problems and achieve the following objects. That is, an object of the present invention is to provide a novel composition having at least one of excellent whitening action and anti-aging action and high safety.
Another object of the present invention is to provide a whitening agent having an excellent whitening action and high safety.
Another object of the present invention is to provide a highly safe anti-aging agent having an excellent anti-aging action.
Another object of the present invention is to provide a highly safe skin cosmetic that has at least one of an excellent whitening action and an anti-aging action.
Another object of the present invention is to provide a highly safe food and drink having at least one of an excellent whitening action and an anti-aging action.

As a result of intensive studies by the present inventors in order to solve the above-mentioned problems, a fermented product of Lactobacillus plantarum ( Lactobacillus plantarum ), which is an extract of Glychyrrhiza glabra , has excellent whitening action and anti-aging. The present invention has been completed by finding that it has an action.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> Fermented product of licorice extract by microorganisms,
The licorice is a Gurichiruriza glabra (Glychyrrhiza glabra),
The licorice extract fermented product is characterized in that the microorganism is Lactobacillus plantarum ( Lactobacillus plantarum ).
<2> A whitening agent containing the fermented licorice extract according to <1>.
<3> The whitening agent according to <2>, which has a tyrosinase activity inhibitory action.
<4> An anti-aging agent comprising the fermented licorice extract according to <1>.
<5> The anti-aging agent according to <4>, wherein the anti-aging agent has at least one selected from the group consisting of a profilagulin mRNA expression promoting action, a serine palmitoyltransferase mRNA expression promoting action, and a hyaluronic acid synthase 3 mRNA expression promoting action. .
<6> Fermented licorice extract according to <1>, whitening agent according to any one of <2> to <3>, and anti-aging agent according to any one of <4> to <5>. It is a skin cosmetic characterized by containing at least 1 sort (s) selected from the group which consists of.
<7> Fermented licorice extract according to <1>, whitening agent according to any one of <2> to <3>, and anti-aging agent according to any one of <4> to <5>. A food or drink comprising at least one selected from the group consisting of:

According to the present invention, it is possible to solve the above-described problems and provide a novel composition having a high safety and having at least one of an excellent whitening action and an anti-aging action.
According to the whitening agent of the present invention, it is possible to solve the conventional problems and provide a whitening agent having an excellent whitening action and high safety.
According to the anti-aging agent of the present invention, it is possible to solve the conventional problems and to provide an anti-aging agent having an excellent anti-aging action and high safety.
According to the skin cosmetic of the present invention, it is possible to solve the conventional problems and to provide a highly safe skin cosmetic having at least one of an excellent whitening action and an anti-aging action.
According to the food / beverage products of this invention, the said various problems in the past can be solved, and the food / beverage products which have at least any one of the outstanding whitening effect | action and anti-aging effect, and can provide high safety | security can be provided.

(Fermented licorice extract)
The fermented licorice extract of the present invention is a fermented product of microorganisms of an extract from licorice.

<Liquorice extract>
Licorice (licorice) is a perennial herb belonging to the genus Glycyrrhiza and has been used as a raw material for medicinal or sweeteners since ancient times.
Licorice of the present invention is a Gurichiruriza glabra (Glychyrrhiza glabra).

As the licorice extract, one prepared from an extraction site used as an extraction raw material may be used, or a commercially available product may be used.
The extraction part of the licorice is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts, etc. Examples include the above-ground part, root part, rhizome part, or a mixture of these parts. Among these, a root part, a leaf part, and a rhizome part are preferable.
The method for preparing the licorice extraction part is not particularly limited and may be appropriately selected depending on the intended purpose. For example, after each part is dried, the part is dried or pulverized using a crusher for solvent extraction. Can be obtained. The drying may be performed in the sun, or may be performed using a commonly used dryer.

  The licorice extract can be easily obtained by a method generally used for plant extraction. There is no restriction | limiting in particular as said licorice extract, According to the objective, it can select suitably, For example, the diluted solution of an extract, a concentrate, the dried product of an extract, a crude refined product, a refined product etc. are mentioned. .

The extraction method of the licorice is not particularly limited and can be appropriately selected according to the purpose. For example, the extraction part of the licorice as an extraction raw material is put into a treatment tank filled with an extraction solvent, and is necessary. Accordingly, there may be mentioned a method of obtaining an extract by eluting soluble components while stirring appropriately and then filtering to remove the extraction residue.
Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction with a polar solvent can be performed efficiently.

  There are no particular limitations on the conditions (extraction time and extraction temperature), the extraction solvent, and the amount used thereof in the extraction of licorice, and it can be appropriately selected according to the purpose.

  There is no restriction | limiting in particular as said extraction solvent, According to the objective, it can select suitably, For example, water, a hydrophilic organic solvent, or these mixed solvents etc. are mentioned.

  The water is not particularly limited and may be appropriately selected according to the purpose.For example, pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, purified water, hot water, Examples include ion-exchanged water, physiological saline, phosphate buffer, and phosphate buffered saline.

  There is no restriction | limiting in particular as said hydrophilic solvent, According to the objective, it can select suitably, For example, C1-C5 lower alcohols, such as methanol, ethanol, propyl alcohol, isopropyl alcohol; Acetone, methyl ethyl ketone, etc. Lower aliphatic ketones; C2-C5 polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, and glycerin.

  There is no restriction | limiting in particular as said mixed solvent, Although it can select suitably according to the objective, When using a lower alcohol, 1 mass part-90 mass parts with respect to 10 mass parts of water, a lower aliphatic ketone. Is used, it is preferable to add 1 part by mass to 90 parts by mass with respect to 10 parts by mass of water.

  The licorice extraction solvent is preferably used at a temperature between room temperature and the boiling point of the solvent.

  The obtained licorice extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method in order to obtain a diluted, concentrated, dried, crudely purified and purified product of the licorice extract. May be.

  The licorice extract prepared as described above is subjected to fermentation by microorganisms described later.

<Fermentation>
The fermentation method is not particularly limited as long as Lactobacillus plantarum is used, and can be appropriately selected from known fermentation methods according to the purpose.

-Lactobacillus plantarum-
The Lactobacillus plantarum is not particularly limited and may be appropriately selected depending on the intended purpose. Here, Lactobacillus plantarum is a kind of lactic acid bacteria, but is mainly included in plant lactic acid bacteria because it is isolated from vegetable fermented foods such as vegetable pickles, kimchi, miso, etc., fermented milk such as yogurt and It is different from animal lactic acid bacteria used for production of lactic acid bacteria beverages. The said Lactobacillus plantarum may be used individually by 1 type, and may use 2 or more types together. When using 2 or more types together, you may perform fermentation simultaneously with 2 or more types of Lactobacillus plantarum, and you may perform fermentation separately.

  Among the Lactobacillus plantarum, Lactobacillus plantarum 22A-1 (FERM P-21409), Lactobacillus plantarum 22A-3 (FERM P-21411), and Lactobacillus plantarum 22B-2 (FERM) It is preferable to use at least one selected from the group consisting of P-21410), and it is particularly preferable to use Lactobacillus plantarum 22A-3 (FERM P-21411). Three strains of the Lactobacillus plantarum 22A-1 (FERM P-21409), Lactobacillus plantarum 22A-3 (FERM P-21411), and Lactobacillus plantarum 22B-2 (FERM P-21410) are , A strain isolated and identified from pickles by the present applicant, applied for deposit at the National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center, and was deposited on October 22, 2007.

-Fermentation treatment-
The fermentation treatment can be performed by, for example, dissolving the concentrated and dried licorice extract in water or using the licorice extract as it is without concentrating to dryness and inoculating the Lactobacillus plantarum.

  The fermentation treatment using the Lactobacillus plantarum is, for example, putting an aqueous solution of licorice extract and the Lactobacillus plantarum into a fermenter and in a temperature range of 25 ° C to 35 ° C, preferably 28 ° C to 32 ° C. For 12 hours to 72 hours, preferably 18 hours to 48 hours. In addition, when the pH of the reaction solution is adjusted to 5.0 to 7.0, preferably 5.5 to 6.5 at the start of the fermentation process, the fermentation process suitably proceeds. Such fermentation treatment is terminated by subjecting the fermentation broth to desired means such as cooling, heat sterilization, and filtration, and inactivating the fermenting bacteria.

  The fermentation broth thus obtained may be used as it is as a fermented product, or may be appropriately diluted or concentrated and used as a fermented product. Furthermore, the concentrate may be further dried, and crude purification, purification, and the like may be performed.

  In addition, you may perform the process for improving extraction efficiency and fermentation efficiency with respect to an extraction raw material or an extract (fermentation raw material) before the extraction process and fermentation process mentioned above. As such a treatment, for example, an enzyme treatment using glucanase, cellulase or the like is preferably exemplified. For example, it is particularly preferable to perform an enzyme treatment on the extract obtained by the extraction treatment because the efficiency of the subsequent fermentation treatment is increased.

The fermented licorice extract obtained in this way exhibits excellent whitening and anti-aging effects, and is therefore suitable as a whitening agent and an anti-aging agent, which will be described later, or as a blending ingredient for skin cosmetics and foods and beverages. Can be used.
The licorice extract fermented product can be used as it is as an active ingredient of a whitening agent, an anti-aging agent, or a skin cosmetic and food / beverage component as described later, but in terms of ease of use, the concentrated solution, The dried product is preferred. In obtaining the dried product, a carrier such as dextrin or cyclodextrin may be added to improve hygroscopicity.

(Whitening agent and anti-aging agent)
The whitening agent and anti-aging agent of this invention contain the fermented licorice extract of this invention mentioned above, and also contain another component as needed.

  The whitening agent has a tyrosinase activity inhibitory action.

  The anti-aging agent has at least one selected from the group consisting of a profilagrin mRNA expression promoting action, a serine palmitoyltransferase mRNA expression promoting action, and a hyaluronic acid synthase 3 mRNA expression promoting action.

  Details of the substance contained in the fermented licorice extract that exhibits at least one of the tyrosinase activity inhibitory action, the profilagrin mRNA expression promoting action, the serine palmitoyltransferase mRNA expression promoting action, and the hyaluronic acid synthase 3 mRNA expression promoting action are unknown However, it has not been known at all that the fermented licorice extract has such an excellent action and is useful as a whitening agent and an anti-aging agent. It is.

<Fermented licorice extract>
The fermented licorice extract is the fermented licorice extract of the present invention described above.
There is no restriction | limiting in particular as content in the whitening agent and anti-aging agent of the said licorice extract fermented material, It can adjust suitably by the physiological activity etc. of the said extract. The whitening agent and anti-aging agent may be composed only of the fermented licorice extract.

<Other ingredients>
There is no restriction | limiting in particular as said other component, According to the utilization form of the said whitening agent and an anti-aging agent, it can select suitably. For example, excipients, moisture-proofing agents, preservatives, strengthening agents, thickeners, emulsifiers, antioxidants, sweeteners, acidulants, seasonings, coloring agents, fragrances, whitening agents, moisturizers, oily ingredients, UV absorption Agents, surfactants, alcohols, powder components, colorants, aqueous components, water, skin nutrients, and the like. These may be used individually by 1 type and may use 2 or more types together.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

<Application>
There is no restriction | limiting in particular as a use of the whitening agent and anti-aging agent of this invention, According to the objective, it can select suitably, For example, a pharmaceutical, a quasi-drug, cosmetics, food-drinks, etc. are mentioned.

  The whitening agent and anti-aging agent of the present invention are preferably applied to humans. However, as long as each effect is exhibited, animals other than humans (for example, mice, rats, hamsters, dogs, Cat, cow, pig, monkey, etc.).

  The whitening agent and anti-aging agent of the present invention can also be used as a reagent for research on the mechanism of whitening action and anti-aging action.

(Skin cosmetic)
The skin cosmetic contains at least one selected from the group consisting of fermented licorice extract, whitening agent and anti-aging agent of the present invention, and further contains other components as necessary.

  The content in the at least one skin cosmetic selected from the group consisting of the fermented licorice extract, whitening agent and anti-aging agent is not particularly limited, and is appropriately adjusted according to the physiological activity of the fermented licorice extract. However, it is preferably 0.0001% by mass to 20% by mass and more preferably 0.0001% by mass to 10% by mass in terms of the fermented licorice extract. The skin cosmetic may be composed of at least one selected from the group consisting of the fermented licorice extract, whitening agent and anti-aging agent.

The other components in the skin cosmetics are not particularly limited and can be appropriately selected according to the purpose from the main ingredients, auxiliaries or other components used in the production of ordinary skin cosmetics, for example, Astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory / antiallergic agents, antioxidant / active oxygen removers, fats and oils, waxes, hydrocarbons, fatty acids, alcohol , Esters, surfactants, fragrances and the like. These may be used individually by 1 type and may use 2 or more types together.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

  The skin cosmetics of the present invention can be used on a daily basis, and various physiologically active actions such as a whitening action and an anti-aging action are extremely effective by the action of the fermented licorice extract which is an active ingredient. Therefore, it can be suitably used for at least one of whitening use and anti-aging use.

  There is no restriction | limiting in particular as a kind of said skin cosmetics, According to the objective, it can select suitably, For example, ointment, cream, milky lotion, cosmetic liquid, lotion, pack, foundation etc. are mentioned.

  The skin cosmetic of the present invention is suitably applied to humans, but as long as the respective effects are exhibited, animals other than humans (for example, mice, rats, hamsters, dogs, cats, cattle) , Pigs, monkeys, etc.).

(Food)
The said food-drinks contain at least 1 sort (s) selected from the group which consists of the fermented licorice extract of this invention, a whitening agent, and an anti-aging agent, and also contains another component as needed.

  Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to categories such as goods. Therefore, the foods and drinks are general foods, health foods (functional foods and drinks), health functional foods (specific health foods, nutritional functional foods, functional foods), quasi drugs, pharmaceuticals Means foods and drinks that make up a wide variety of foods.

  There is no restriction | limiting in particular as said kind of food / beverage products, According to the objective, it can select suitably, For example, drinks, such as a tea drink, a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, Ice confectionery such as ice sherbet and shaved ice; noodles such as buckwheat, udon, harusame, gyoza peel, cucumber peel, Chinese noodles, instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, Sweets such as cream, baked goods, bread; seafood such as crabs, salmon, clams, tuna, sardines, shrimp, bonito, mackerel, whales, oysters, saury, squid, scallop, scallop, abalone, sea urchin, crabs, tokobushi; Fish and fishery processed foods such as kamaboko, ham and sausage; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise , Shortening, whipped cream, dressing and other fats and oils and processed foods; sauces, sauces and other seasonings; curry, stew, oyakodon, rice bowl, miscellaneous cooking, Chinese rice bowl, bowl, tempura, eel rice, hayashi rice, oden, mabodorf , Beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs and other retort pouch foods; salads, pickles and other side dishes; various forms of health, beauty and nutritional supplements; tablets, granules, capsules Drugs, drinks, troches and other pharmaceuticals, quasi drugs and the like.

  The content in at least one food and drink selected from the group consisting of the fermented licorice extract, whitening agent and anti-aging agent is not particularly limited and may be appropriately changed in consideration of the purpose of use, symptoms, sex and the like. For example, in consideration of the general intake of foods and drinks to be added, the daily intake of fermented fermented licorice is about 1 mg to 1,000 mg per adult. preferable. In addition, when the food or drink to be added is a granular, tablet or capsule food or drink, at least one addition amount selected from the group consisting of the fermented licorice extract, whitening agent and anti-aging agent is added It is 0.1-100 mass% normally with respect to food-drinks, Preferably it is 5-100 mass%.

The other components in the food and drink are not particularly limited and can be appropriately selected according to the purpose from auxiliary ingredients or additives or other components used in the production of normal food and drink. Glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, Propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, gum arabic, carrageenan, casein, gelatin, pectin, agar, vitamin B, nicotinamide, calcium pantothenate, amino acids, cal Umm salts, dyes, perfumes, and the like preservatives. These may be used individually by 1 type and may use 2 or more types together.
There is no restriction | limiting in particular as a compounding quantity of the said other component, According to the objective, it can select suitably.

  The food and drink of the present invention can be taken orally on a daily basis, and various physiological activities such as a whitening action and an anti-aging action are extremely effective by the action of the fermented licorice extract which is an active ingredient. Therefore, it can be suitably used for at least one of whitening use and anti-aging use.

  The food and drink of the present invention is suitably applied to humans, but as long as the respective effects are exhibited, animals other than humans (for example, mice, rats, hamsters, dogs, cats, cows, It can also be applied to pigs, monkeys, etc.).

  Hereinafter, although the manufacture example and test example of this invention are demonstrated, this invention is not limited to these manufacture example and test example at all.

(Comparative Production Example 1)
- production of water extract of Gurichiruriza glabra (G. glabra) -
As a raw material for extraction, 30 g of a pulverized product of glycyrrhiza grabra ( G. glabra ) root was added to 1,000 mL of water, and then sterilized to obtain a glycyrrhiza grabra ( G. glabra ) extract (hereinafter referred to as “ G. glabra ”). (Sometimes referred to as “no fermentation liquor”).

(Production Example 1)
-Production of G. glabra extract fermented product-
G. obtained in Comparative Production Example 1 Lactobacillus plantarum ( Lactobacillus plantarum ) 22A-3 strain (Accession No .: FERM P-21411) was inoculated into a non- glabra fermentation solution (1,000 g) and fermented at 30 ° C. for 24 hours. The obtained fermentation broth was filtered, and the filtrate was concentrated and dried to obtain a glycyrrhiza grabra ( G. glabra ) extract fermented product (hereinafter sometimes referred to as “ G. glabra fermentation broth”). 11g).

(Comparative Production Example 2)
-Production of water extract of G. uralensis-
Comparative Production Example 1, except for changing the pulverized root of Gurichiruriza glabra (G. glabra) the pulverized root of Gurichiruriza-uralensis (G. uralensis) in the same manner, Gurichiruriza-uralensis (G. uralensis) An extract (hereinafter, sometimes referred to as “ G. uralensis no-fermentation liquid”) was obtained.

(Comparative Production Example 3)
-Production of G. uralensis extract fermented product-
In Production Example 1, G. G. glabra fermentation-free liquid obtained in Comparative Production Example 2 was obtained . A glycyrrhiza urarensis ( G. uralensis ) extract fermented product (hereinafter sometimes referred to as “ G. uralensis fermented liquid”) was obtained in the same manner except that the urelensis fermentation liquid was not used.

(Test Example 1: Tyrosinase activity inhibitory action test)
Using the extract or the fermented extract obtained in Production Example 1 and Comparative Production Examples 1 to 3 as test samples, the tyrosinase activity inhibitory action was tested by the following test method.

Test samples dissolved in 0.2 ml of Mclvaine buffer (pH 6.8), 0.06 mL of 0.3 mg / mL tyrosine solution, and 25% DMSO solution in 48 well plates (sample concentrations are shown in Tables 1-1 and 1-2 below) 0.18 mL was added and allowed to stand at 37 ° C. for 10 minutes. To this, 0.02 mL of 800 units / mL tyrosinase solution was added, followed by reaction at 37 ° C. for 15 minutes. After completion of the reaction, the absorbance at a wavelength of 475 nm was measured.
Further, as a blank, the same operation and absorbance measurement were performed when no enzyme solution was added. Further, as a control, the same measurement was performed when distilled water was added without adding the sample solution. From the obtained measurement results, the tyrosinase activity inhibition rate (%) was calculated by the following formula 1. The results are shown in Tables 1-1 and 1-2.
<Formula 1>
Tyrosinase activity inhibition rate (%) = {1− (A−B) / (C−D)} × 100
A to D in Formula 1 each represent the following.
A: Absorbance at a wavelength of 475 nm when a test sample is added and an enzyme is added B: Absorbance at a wavelength of 475 nm when a test sample is added and an enzyme is not added C: Absorbance at a wavelength of 475 nm when no sample is added and an enzyme is added D: No sample is added and an enzyme Absorbance at a wavelength of 475 nm without addition

From the results of Tables 1-1 and 1-2, by fermenting Gurichiruriza glabra (Glychyrrhiza glabra) extract by Lactobacillus plantarum (Lactobacillus plantarum), the licorice extract fermentation product having an excellent tyrosinase inhibitory activity It was confirmed that it was obtained.

(Test Example 2: Profilagrin mRNA expression promoting effect test)
Using the extract or the fermented extract obtained in Production Example 1 and Comparative Production Examples 1 to 3 as a test sample, the profilaggrin mRNA expression promoting effect was tested by the following test method.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells were seeded in 2 mL each in a 35 mm dish using KGM (40 × 10 ⁴ cells / dish) and cultured overnight under conditions of 37 ° C. and 5% CO ₂ .
After culturing, the medium was replaced with normal human epidermal keratinocyte basal medium (KBM, the above-mentioned KGM medium without growth factors (hEGF, BPE, insulin) added). After 24 hours, the culture solution was discarded, and 2 mL of each test sample (see Table 2 below for sample concentration) dissolved in KBM was added to each dish and cultured for 24 hours under conditions of 37 ° C. and 5% CO ₂ . In addition, as a control, the same culture was performed using a KBM medium to which no test sample was added.
After culturing, the culture solution is discarded, total RNA is extracted with ISOGEN II (Nippon Gene, Cat. No. 311-07361), and the amount of each RNA is measured with a spectrophotometer so that it becomes 200 ng / μL. Total RNA was prepared.
Using this total RNA as a template, the expression level of profilaggrin and the internal standard GAPDH mRNA was measured. Detection is carried out using a real-time PCR device Smart Cycler (registered trademark) (manufactured by Cepheid), TaKaRa SYBR (registered trademark) Prime Script (registered trademark) RT-PCR kit (Perfect Real Time) (produced by Takara Bio Inc., code No. RR063A). ) By real-time two-step RT-PCR reaction. The expression level of profilagrin mRNA was determined as a correction value based on the value of GAPDH mRNA based on the total RNA preparation prepared from the cells cultured in “addition of test sample” and “no addition of test sample”, respectively. From the obtained value, the profilaggrin mRNA expression promotion rate (%) was calculated by the following formula 2. The results are shown in Table 2.
<Formula 2>
Profilaggrin mRNA expression promotion rate (%) = A / B × 100
AB in Formula 2 represents the following, respectively.
A: Correction value when test sample is added B: Correction value when test sample is not added

From the results shown in Table 2, by fermenting Gurichiruriza glabra (Glychyrrhiza glabra) extract by Lactobacillus plantarum (Lactobacillus plantarum), that licorice extract fermentation product having excellent profilaggrin mRNA expression promoting effect can be obtained confirmed.

(Test Example 3: Serine palmitoyltransferase (SPT) mRNA expression promoting action test)
The extract or fermented fermented product obtained in Production Example 1 and Comparative Production Examples 1 to 3 was used as a test sample, and the serine palmitoyltransferase (SPT) mRNA expression promoting effect was tested by the following test method.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells were seeded in 2 mL each in a 35 mm dish using KGM (40 × 10 ⁴ cells / dish) and cultured overnight under conditions of 37 ° C. and 5% CO ₂ .
After culturing, the medium was replaced with normal human epidermal keratinocyte basal medium (KBM, the above-mentioned KGM medium without growth factors (hEGF, BPE, insulin) added). After 24 hours, the culture solution was discarded, and 2 mL of each test sample (see Table 3 below for sample concentration) dissolved in KBM was added to each dish and cultured for 24 hours under conditions of 37 ° C. and 5% CO ₂ . In addition, as a control, the same culture was performed using a KBM medium to which no test sample was added.
After culturing, the culture solution is discarded, total RNA is extracted with ISOGEN II (Nippon Gene, Cat. No. 311-07361), and the amount of each RNA is measured with a spectrophotometer so that it becomes 200 ng / μL. Total RNA was prepared.
Using this total RNA as a template, the expression level of SPT and mRNA of GAPDH as an internal standard was measured. Detection is carried out using a real-time PCR device Smart Cycler (registered trademark) (manufactured by Cepheid), TaKaRa SYBR (registered trademark) Prime Script (registered trademark) RT-PCR kit (Perfect Real Time) (produced by Takara Bio Inc., code No. RR063A). ) By real-time two-step RT-PCR reaction. As for the expression level of SPT mRNA, a correction value was obtained from the value of GAPDH mRNA based on the total RNA preparation prepared from the cells cultured in “addition of test sample” and “no test sample”, respectively. From the obtained value, the SPT mRNA expression promotion rate (%) was calculated by the following formula 3. The results are shown in Table 3.
<Formula 3>
SPT mRNA expression promotion rate (%) = A / B × 100
AB in Formula 3 represents the following, respectively.
A: Correction value when test sample is added B: Correction value when test sample is not added

From the results shown in Table 3, by fermenting Gurichiruriza glabra (Glychyrrhiza glabra) extract by Lactobacillus plantarum (Lactobacillus plantarum), the licorice extract fermentation product having excellent serine palmitoyltransferase (SPT) mRNA expression promoting effect It was confirmed that it was obtained.

(Test Example 4: Hyaluronic acid synthase 3 (HAS3) mRNA expression promoting action test)
The extract or fermented extract obtained in Production Example 1 and Comparative Production Example 1 was used as a test sample, and hyaluronic acid synthase 3 (HAS3) mRNA expression promoting action was tested by the following test method.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells were seeded in 2 mL each in a 35 mm dish using KGM (40 × 10 ⁴ cells / dish) and cultured overnight under conditions of 37 ° C. and 5% CO ₂ .
After culturing, the medium was replaced with normal human epidermal keratinocyte basal medium (KBM, the above-mentioned KGM medium without growth factors (hEGF, BPE, insulin) added). After 24 hours, the culture solution was discarded, and 2 mL of each test sample (see Table 4 below for sample concentration) dissolved in KBM was added to each dish and cultured for 24 hours under conditions of 37 ° C. and 5% CO ₂ . As a control, the same culture was performed using a KBM medium to which no test sample was added.
After culturing, the culture solution is discarded, and total RNA is extracted with ISOGEN II (Nippon Gene, Cat. No. 311-07361), and the amount of each RNA is measured with a spectrophotometer to be 200 ng / μL. Total RNA was prepared.
Using this total RNA as a template, the expression level of mRNA of HAS3 and GAPDH as an internal standard was measured. Detection is performed using a real-time PCR device Smart Cycler (registered trademark) (manufactured by Cepheid), TaKaRa SYBR (registered trademark) Prime Script (registered trademark) RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio Inc., code No. RR063A). ) By real-time two-step RT-PCR reaction. The expression level of HAS3 mRNA was determined as a correction value based on the value of GAPDH mRNA based on the total RNA preparation prepared from the cells cultured in “addition of test sample” and “no test sample added”, respectively. From the obtained value, HAS3 mRNA expression promotion rate (%) was calculated by the following formula 4. The results are shown in Table 4.
<Formula 4>
HAS3 mRNA expression promotion rate (%) = A / B × 100
AB in Formula 4 represents the following, respectively.
A: Correction value when test sample is added B: Correction value when test sample is not added

From the results of Table 4, by fermenting Gurichiruriza glabra (Glychyrrhiza glabra) extract by Lactobacillus plantarum (Lactobacillus plantarum), licorice extract fermented with excellent hyaluronan synthase 3 (HAS3) mRNA expression promoting effect It was confirmed that a product was obtained.

(Formulation example 1)
An emulsion having the following composition was produced by a conventional method.
・G. Glabra extract fermented product (Production Example 1) 0.01 g
・ Jojoba oil 4.00 g
・ 1,3-butylene glycol 3.00 g
・ Arbutin 3.00 g
・ Polyoxyethylene cetyl ether (20E.O.) 2.50 g
・ 2.00 g of olive oil
・ Squalane 2.00g
・ Cetanol 2.00g
・ Glyceryl monostearate 2.00g
・ Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00g
・ 0.15 g of methyl paraoxybenzoate
・ Stearyl glycyrrhizinate 0.10g
・ Twilight extract 0.10g
・ Dipotassium glycyrrhizinate 0.10g
・ Ginkgo biloba extract 0.10g
・ Conchiolin 0.10g
・ Oat extract 0.10g
・ Chamomile extract 0.10g
・ Fragrance 0.05g
・ Purified water remainder (total amount is 100 g)

(Formulation example 2)
A cream having the following composition was produced by a conventional method.
・G. Glabra extract fermented product (Production Example 1) 0.05 g
・ Kujin extract 0.1g
・ Ogon Extract 0.1g
・ Liquid paraffin 5.0g
・ Salami beeswax 4.0g
・ Squalane 10.0g
・ Cetanol 3.0g
・ Lanolin 2.0g
・ Stearic acid 1.0g
・ Oleic acid polyoxyethylene sorbitan (20EO) 1.5g
・ 3.0 g of glyceryl monostearate
・ Oil-soluble licorice extract 0.1g
・ 1,3-butylene glycol 6.0 g
・ 1.5 g of methyl paraoxybenzoate
・ Fragrance 0.1g
・ Purified water remainder (total amount is 100 g)

(Formulation example 3)
A serum having the following composition was produced by a conventional method.
・G. Glabra extract fermented product (Production Example 1) 0.01 g
・ Chamomile extract 0.1g
・ Carrot extract 0.1g
・ Xanthan gum 0.3g
・ Hydroxyethylcellulose 0.1g
・ Carboxyvinyl polymer 0.1g
・ 1,3-butylene glycol 4.0 g
・ Dipotassium glycyrrhizinate 0.1g
・ Glycerin 2.0g
・ Potassium hydroxide 0.25g
・ Fragrance 0.01g
・ Preservative (methyl paraoxybenzoate) 0.15g
・ Ethanol 2.0g
・ Purified water remainder (total amount is 100 g)

(Formulation example 4)
Tablets having the following composition were produced by a conventional method.
・G. Glabra extract fermented product (Production Example 1) 5.0 mg
・ Dolomite (containing 20% calcium and 10% magnesium) 83.4mg
・ Casein phosphopeptide 16.7mg
・ Vitamin C 33.4mg
・ Maltitol 136.8mg
・ Collagen 12.7mg
・ Sucrose fatty acid ester 12.0mg

(Formulation example 5)
An oral liquid preparation having the following composition was produced by a conventional method.
・G. Glabra extract fermented product (Production Example 1) 0.3% by mass
・ Sorbit 12.0% by mass
・ Sodium benzoate 0.1% by mass
・ Fragrance 1.0% by mass
・ Calcium sulfate 0.5 mass%
・ Purified water balance (100% by mass)

FERM P-21409
FERM P-21411
FERM P-21410

Claims (7)

A fermented product of licorice extract by microorganisms,
The licorice is a Gurichiruriza glabra (Glychyrrhiza glabra),
The fermented licorice extract, wherein the microorganism is Lactobacillus plantarum ( Lactobacillus plantarum ).   A whitening agent comprising the fermented licorice extract according to claim 1.   The whitening agent according to claim 2, which has a tyrosinase activity inhibitory action.   An anti-aging agent comprising the fermented licorice extract according to claim 1.   The anti-aging agent according to claim 4, which has at least one selected from the group consisting of a profilagrin mRNA expression promoting action, a serine palmitoyltransferase mRNA expression promoting action and a hyaluronic acid synthase 3 mRNA expression promoting action.   A fermented licorice extract according to claim 1, at least one selected from the group consisting of a whitening agent according to any one of claims 2 to 3 and an anti-aging agent according to any one of claims 4 to 5. A skin cosmetic characterized by containing.   A fermented licorice extract according to claim 1, at least one selected from the group consisting of a whitening agent according to any one of claims 2 to 3 and an anti-aging agent according to any one of claims 4 to 5. A food or drink characterized by containing.