JP4537573B2 - 熱安定ルシフェラーゼと生産方法 - Google Patents

熱安定ルシフェラーゼと生産方法 Download PDF

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Publication number
JP4537573B2
JP4537573B2 JP2000511874A JP2000511874A JP4537573B2 JP 4537573 B2 JP4537573 B2 JP 4537573B2 JP 2000511874 A JP2000511874 A JP 2000511874A JP 2000511874 A JP2000511874 A JP 2000511874A JP 4537573 B2 JP4537573 B2 JP 4537573B2
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luciferase
synthetic
plate
mutagenesis
beetle
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JP2001516585A (ja
JP2001516585A5 (enExample
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ウツド，キース・ブイ
ホール，メアリー・ピー
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プロメガ・コーポレイシヨン
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000511874A 1997-09-19 1998-09-18 熱安定ルシフェラーゼと生産方法 Expired - Lifetime JP4537573B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5937997P 1997-09-19 1997-09-19
US60/059,379 1997-09-19
PCT/US1998/019494 WO1999014336A2 (en) 1997-09-19 1998-09-18 Thermostable luciferases and methods of production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2010006999A Division JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

Publications (3)

Publication Number Publication Date
JP2001516585A JP2001516585A (ja) 2001-10-02
JP2001516585A5 JP2001516585A5 (enExample) 2006-01-05
JP4537573B2 true JP4537573B2 (ja) 2010-09-01

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Family Applications (2)

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JP2000511874A Expired - Lifetime JP4537573B2 (ja) 1997-09-19 1998-09-18 熱安定ルシフェラーゼと生産方法
JP2010006999A Expired - Lifetime JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

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JP2010006999A Expired - Lifetime JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

Country Status (6)

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US (1) US20030068801A1 (enExample)
EP (2) EP2860252A1 (enExample)
JP (2) JP4537573B2 (enExample)
AU (1) AU754312C (enExample)
CA (1) CA2301828A1 (enExample)
WO (1) WO1999014336A2 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
WO2001004310A1 (en) * 1999-07-13 2001-01-18 Heska Corporation Fc EPSILON RECEPTOR-LUMINESCENCE INDUCING PROTEIN CHIMERIC NUCLEIC ACID MOLECULES, FUSION PROTEINS AND USES THEREOF
CN100516223C (zh) * 1999-10-26 2009-07-22 3M创新有限公司 新型酶
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7083911B2 (en) 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
EP1546374B1 (en) 2002-09-06 2010-11-10 Promega Corporation Method for detecting transferase enzymatic activity
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
US7741067B2 (en) 2002-12-23 2010-06-22 Promega Corporation Luciferase-based assays
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
EP2308978B8 (en) 2003-10-10 2015-04-08 Promega Corporation Luciferase biosensor
ES2324952T3 (es) * 2004-07-02 2009-08-20 Promega Corporation Composiciones y procesos para la extraccion y deteccion de atp microbiano.
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0505763D0 (en) * 2005-03-22 2005-04-27 Enigma Diagnostics Ltd Polymorphism detection method
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
US9045730B2 (en) * 2008-05-19 2015-06-02 Promega Corporation Luciferase biosensors for cAMP
CN102159948B (zh) 2008-07-22 2014-06-25 普罗美加公司 基于adp检测的发光磷酸转移酶或atp水解酶测定
SG10201901453PA (en) 2009-05-01 2019-03-28 Promega Corp Synthetic oplophorus luciferases with enhanced light output
JP5961163B2 (ja) 2010-05-11 2016-08-02 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された検出特性を有する変異プロテアーゼバイオセンサー
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
BR112013010855A2 (pt) 2010-11-02 2017-06-27 Promega Corp luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso
SG189494A1 (en) 2010-11-02 2013-05-31 Promega Corp Coelenterazine derivatives and methods of using same
EP2753928B1 (en) * 2011-09-09 2018-02-07 Quidel Corporation Compositions and methods for detecting autoantibodies
CN111004835B (zh) 2013-02-22 2023-12-29 普洛麦格公司 用于荧光素酶和核苷磷酸的发光检测的稳定制剂
EP3099691B1 (en) 2014-01-29 2019-11-20 Promega Corporation Pro-substrates for live cell applications
EP3099683B1 (en) 2014-01-29 2020-08-05 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
EP3204509B1 (en) 2014-10-08 2019-07-10 Promega Corporation Bioluminescent succinate detection assay
JP6688499B2 (ja) * 2014-10-27 2020-04-28 国立大学法人 鹿児島大学 安定性が増加したウミシイタケ（Ｒｅｎｉｌｌａｒｅｎｉｆｏｒｍｉｓ）由来ルシフェラーゼ変異体
CN105200022B (zh) * 2015-11-03 2018-11-16 南京工业大学 萤火虫荧光素酶热稳定性改造的方法
EP3219722B1 (en) * 2016-03-14 2019-03-27 Hung Guang Biotech Co. Ltd. Modified colostrum protein and application thereof
US20220064606A9 (en) * 2018-07-06 2022-03-03 Kikkoman Corporation Luciferase variant
CN110456052A (zh) * 2019-08-22 2019-11-15 中国科学院武汉病毒研究所 一种血清中梅毒螺旋体抗体的快速检测方法及其应用
EP4656723A1 (en) 2023-01-27 2025-12-03 Kikkoman Corporation Luciferase variant

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5229285A (en) * 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
JP3048466B2 (ja) * 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases
US6171808B1 (en) * 1995-01-20 2001-01-09 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Mutant luciferases
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production

Also Published As

Publication number Publication date
WO1999014336A2 (en) 1999-03-25
WO1999014336A3 (en) 1999-06-03
US20030068801A1 (en) 2003-04-10
JP2001516585A (ja) 2001-10-02
AU754312C (en) 2005-11-10
AU754312B2 (en) 2002-11-14
AU9492198A (en) 1999-04-05
EP1015601B1 (en) 2015-01-07
EP1015601A2 (en) 2000-07-05
EP2860252A1 (en) 2015-04-15
JP2010104377A (ja) 2010-05-13
CA2301828A1 (en) 1999-03-25
JP5580607B2 (ja) 2014-08-27

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