CA2301828A1 - Thermostable luciferases and methods of production - Google Patents
Thermostable luciferases and methods of production Download PDFInfo
- Publication number
- CA2301828A1 CA2301828A1 CA002301828A CA2301828A CA2301828A1 CA 2301828 A1 CA2301828 A1 CA 2301828A1 CA 002301828 A CA002301828 A CA 002301828A CA 2301828 A CA2301828 A CA 2301828A CA 2301828 A1 CA2301828 A1 CA 2301828A1
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- Prior art keywords
- luciferase
- synthetic
- beetle
- plate
- luciferases
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000003113 dilution method Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000010429 evolutionary process Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000007422 luminescence assay Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
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- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 230000000858 peroxisomal effect Effects 0.000 description 1
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- 230000014639 sexual reproduction Effects 0.000 description 1
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- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
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- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
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- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5937997P | 1997-09-19 | 1997-09-19 | |
| US60/059,379 | 1997-09-19 | ||
| PCT/US1998/019494 WO1999014336A2 (en) | 1997-09-19 | 1998-09-18 | Thermostable luciferases and methods of production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2301828A1 true CA2301828A1 (en) | 1999-03-25 |
Family
ID=22022584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002301828A Abandoned CA2301828A1 (en) | 1997-09-19 | 1998-09-18 | Thermostable luciferases and methods of production |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20030068801A1 (enExample) |
| EP (2) | EP2860252A1 (enExample) |
| JP (2) | JP4537573B2 (enExample) |
| AU (1) | AU754312C (enExample) |
| CA (1) | CA2301828A1 (enExample) |
| WO (1) | WO1999014336A2 (enExample) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602677B1 (en) * | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| CA2301828A1 (en) * | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| WO2001004310A1 (en) * | 1999-07-13 | 2001-01-18 | Heska Corporation | Fc EPSILON RECEPTOR-LUMINESCENCE INDUCING PROTEIN CHIMERIC NUCLEIC ACID MOLECULES, FUSION PROTEINS AND USES THEREOF |
| CN100516223C (zh) * | 1999-10-26 | 2009-07-22 | 3M创新有限公司 | 新型酶 |
| GB9925161D0 (en) | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| US7083911B2 (en) | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| EP1546374B1 (en) | 2002-09-06 | 2010-11-10 | Promega Corporation | Method for detecting transferase enzymatic activity |
| US7413874B2 (en) | 2002-12-09 | 2008-08-19 | University Of Miami | Nucleic acid encoding fluorescent proteins from aquatic species |
| US7741067B2 (en) | 2002-12-23 | 2010-06-22 | Promega Corporation | Luciferase-based assays |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| EP2308978B8 (en) | 2003-10-10 | 2015-04-08 | Promega Corporation | Luciferase biosensor |
| ES2324952T3 (es) * | 2004-07-02 | 2009-08-20 | Promega Corporation | Composiciones y procesos para la extraccion y deteccion de atp microbiano. |
| GB0416944D0 (en) | 2004-07-29 | 2004-09-01 | Lumora Ltd | Method for determining the amount of template nucleic acid present in a sample |
| US7728118B2 (en) | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| GB0505763D0 (en) * | 2005-03-22 | 2005-04-27 | Enigma Diagnostics Ltd | Polymorphism detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| JP4999341B2 (ja) * | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
| EP2327768B1 (en) | 2006-04-03 | 2015-09-09 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| US9045730B2 (en) * | 2008-05-19 | 2015-06-02 | Promega Corporation | Luciferase biosensors for cAMP |
| CN102159948B (zh) | 2008-07-22 | 2014-06-25 | 普罗美加公司 | 基于adp检测的发光磷酸转移酶或atp水解酶测定 |
| SG10201901453PA (en) | 2009-05-01 | 2019-03-28 | Promega Corp | Synthetic oplophorus luciferases with enhanced light output |
| JP5961163B2 (ja) | 2010-05-11 | 2016-08-02 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 増強された検出特性を有する変異プロテアーゼバイオセンサー |
| US9290794B2 (en) | 2010-05-11 | 2016-03-22 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
| BR112013010855A2 (pt) | 2010-11-02 | 2017-06-27 | Promega Corp | luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso |
| SG189494A1 (en) | 2010-11-02 | 2013-05-31 | Promega Corp | Coelenterazine derivatives and methods of using same |
| EP2753928B1 (en) * | 2011-09-09 | 2018-02-07 | Quidel Corporation | Compositions and methods for detecting autoantibodies |
| CN111004835B (zh) | 2013-02-22 | 2023-12-29 | 普洛麦格公司 | 用于荧光素酶和核苷磷酸的发光检测的稳定制剂 |
| EP3099691B1 (en) | 2014-01-29 | 2019-11-20 | Promega Corporation | Pro-substrates for live cell applications |
| EP3099683B1 (en) | 2014-01-29 | 2020-08-05 | Promega Corporation | Quinone-masked probes as labeling reagents for cell uptake measurements |
| WO2016040788A1 (en) | 2014-09-11 | 2016-03-17 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
| EP3204509B1 (en) | 2014-10-08 | 2019-07-10 | Promega Corporation | Bioluminescent succinate detection assay |
| JP6688499B2 (ja) * | 2014-10-27 | 2020-04-28 | 国立大学法人 鹿児島大学 | 安定性が増加したウミシイタケ（Ｒｅｎｉｌｌａｒｅｎｉｆｏｒｍｉｓ）由来ルシフェラーゼ変異体 |
| CN105200022B (zh) * | 2015-11-03 | 2018-11-16 | 南京工业大学 | 萤火虫荧光素酶热稳定性改造的方法 |
| EP3219722B1 (en) * | 2016-03-14 | 2019-03-27 | Hung Guang Biotech Co. Ltd. | Modified colostrum protein and application thereof |
| US20220064606A9 (en) * | 2018-07-06 | 2022-03-03 | Kikkoman Corporation | Luciferase variant |
| CN110456052A (zh) * | 2019-08-22 | 2019-11-15 | 中国科学院武汉病毒研究所 | 一种血清中梅毒螺旋体抗体的快速检测方法及其应用 |
| EP4656723A1 (en) | 2023-01-27 | 2025-12-03 | Kikkoman Corporation | Luciferase variant |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5229285A (en) * | 1991-06-27 | 1993-07-20 | Kikkoman Corporation | Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly |
| JP3048466B2 (ja) * | 1991-06-27 | 2000-06-05 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法 |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| GB9501170D0 (en) * | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
| US6171808B1 (en) * | 1995-01-20 | 2001-01-09 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Mutant luciferases |
| GB9707486D0 (en) * | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
| CA2301828A1 (en) * | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
-
1998
- 1998-09-18 CA CA002301828A patent/CA2301828A1/en not_active Abandoned
- 1998-09-18 EP EP20140196073 patent/EP2860252A1/en not_active Withdrawn
- 1998-09-18 AU AU94921/98A patent/AU754312C/en not_active Ceased
- 1998-09-18 JP JP2000511874A patent/JP4537573B2/ja not_active Expired - Lifetime
- 1998-09-18 EP EP98948328.4A patent/EP1015601B1/en not_active Expired - Lifetime
- 1998-09-18 WO PCT/US1998/019494 patent/WO1999014336A2/en not_active Ceased
-
2001
- 2001-04-19 US US09/838,469 patent/US20030068801A1/en not_active Abandoned
-
2010
- 2010-01-15 JP JP2010006999A patent/JP5580607B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999014336A2 (en) | 1999-03-25 |
| JP4537573B2 (ja) | 2010-09-01 |
| WO1999014336A3 (en) | 1999-06-03 |
| US20030068801A1 (en) | 2003-04-10 |
| JP2001516585A (ja) | 2001-10-02 |
| AU754312C (en) | 2005-11-10 |
| AU754312B2 (en) | 2002-11-14 |
| AU9492198A (en) | 1999-04-05 |
| EP1015601B1 (en) | 2015-01-07 |
| EP1015601A2 (en) | 2000-07-05 |
| EP2860252A1 (en) | 2015-04-15 |
| JP2010104377A (ja) | 2010-05-13 |
| JP5580607B2 (ja) | 2014-08-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |