JP4448673B2 - 細菌分析装置および方法 - Google Patents
細菌分析装置および方法 Download PDFInfo
- Publication number
- JP4448673B2 JP4448673B2 JP2003299208A JP2003299208A JP4448673B2 JP 4448673 B2 JP4448673 B2 JP 4448673B2 JP 2003299208 A JP2003299208 A JP 2003299208A JP 2003299208 A JP2003299208 A JP 2003299208A JP 4448673 B2 JP4448673 B2 JP 4448673B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteria
- sample
- fermenting
- measurement
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- 238000000034 method Methods 0.000 title claims description 21
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- 238000004458 analytical method Methods 0.000 claims description 34
- 230000001580 bacterial effect Effects 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 27
- 238000002360 preparation method Methods 0.000 claims description 22
- 239000002245 particle Substances 0.000 claims description 18
- 238000010186 staining Methods 0.000 claims description 17
- 230000002378 acidificating effect Effects 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 12
- 239000000975 dye Substances 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
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- 238000012360 testing method Methods 0.000 description 28
- 239000007788 liquid Substances 0.000 description 24
- 239000003153 chemical reaction reagent Substances 0.000 description 16
- 235000000346 sugar Nutrition 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 11
- 210000002700 urine Anatomy 0.000 description 10
- 239000002609 medium Substances 0.000 description 8
- 239000003085 diluting agent Substances 0.000 description 7
- 150000008163 sugars Chemical class 0.000 description 7
- 238000012545 processing Methods 0.000 description 6
- 241000304886 Bacilli Species 0.000 description 5
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- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
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- 241000588724 Escherichia coli Species 0.000 description 3
- 241000495778 Escherichia faecalis Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 208000001388 Opportunistic Infections Diseases 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
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- 241000588626 Acinetobacter baumannii Species 0.000 description 2
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- 206010059866 Drug resistance Diseases 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 239000003978 infusion fluid Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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- 230000020477 pH reduction Effects 0.000 description 1
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- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- IIACRCGMVDHOTQ-UHFFFAOYSA-N sulfamic acid Chemical compound NS(O)(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003299208A JP4448673B2 (ja) | 2003-08-22 | 2003-08-22 | 細菌分析装置および方法 |
| US10/922,522 US7582473B2 (en) | 2003-08-22 | 2004-08-19 | Apparatus and method for analyzing bacteria |
| EP04019716A EP1508621B1 (en) | 2003-08-22 | 2004-08-19 | Apparatus and method for analyzing bacteria |
| DE602004004708T DE602004004708T2 (de) | 2003-08-22 | 2004-08-19 | Vorrichtung und Verfahren zur Bestimmung von Bakterien |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003299208A JP4448673B2 (ja) | 2003-08-22 | 2003-08-22 | 細菌分析装置および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005065573A JP2005065573A (ja) | 2005-03-17 |
| JP2005065573A5 JP2005065573A5 (enExample) | 2006-10-05 |
| JP4448673B2 true JP4448673B2 (ja) | 2010-04-14 |
Family
ID=34056275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003299208A Expired - Fee Related JP4448673B2 (ja) | 2003-08-22 | 2003-08-22 | 細菌分析装置および方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7582473B2 (enExample) |
| EP (1) | EP1508621B1 (enExample) |
| JP (1) | JP4448673B2 (enExample) |
| DE (1) | DE602004004708T2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4236893B2 (ja) * | 2002-10-04 | 2009-03-11 | シスメックス株式会社 | 菌計数方法および菌計数装置 |
| US20100028853A1 (en) * | 2005-10-25 | 2010-02-04 | Harold James Harmon | Optical determination of living vs. non living cells |
| US20070141597A1 (en) * | 2005-10-25 | 2007-06-21 | Harmon H J | Biomimetic Biodetector of Toxins, Viruses, Bacteria, and Biological Factors |
| JP5127606B2 (ja) * | 2008-07-08 | 2013-01-23 | シスメックス株式会社 | 細菌分析装置 |
| US8512975B2 (en) * | 2008-07-24 | 2013-08-20 | Biomerieux, Inc. | Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements |
| EP2472247A4 (en) * | 2009-08-27 | 2013-03-06 | Sharp Kk | DISPLAY CONTROL DEVICE |
| EP2348301B1 (en) * | 2010-01-08 | 2013-08-21 | Sysmex Corporation | Sample analyzer |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5236827A (en) | 1988-06-20 | 1993-08-17 | Becton, Dickinson And Company | Device for enhancing fluorescence and kinetics and methods of using the device |
| JP3067849B2 (ja) * | 1991-07-29 | 2000-07-24 | シスメックス株式会社 | 白血球分類計数用試料調製方法 |
| US5656499A (en) * | 1994-08-01 | 1997-08-12 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| US5897993A (en) * | 1995-03-28 | 1999-04-27 | Idemitsu Kosan Company Limited | Method of determining the number of bacteria quickly and a device for determining the number of bacteria |
| US6070093A (en) * | 1997-12-02 | 2000-05-30 | Abbott Laboratories | Multiplex sensor and method of use |
| US6911313B2 (en) * | 1998-02-06 | 2005-06-28 | Sysmex Corporation | Process for discriminating and counting erythroblasts |
| US7001733B1 (en) * | 1998-05-12 | 2006-02-21 | Rigel Pharmaceuticals, Inc. | Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement |
| DE19921999C2 (de) * | 1999-05-12 | 2003-02-13 | Fraunhofer Ges Forschung | Vorrichtung und Verfahren zur Überwachung und Kontrolle von biologisch aktiven Fluiden |
| JP3837006B2 (ja) | 2000-03-22 | 2006-10-25 | シスメックス株式会社 | 細菌の染色方法及び検出方法 |
| AU2001271438A1 (en) | 2000-06-23 | 2002-01-08 | Daniel Armstrong | Method for separation, identification and evaluation of microbes and cells |
| US7309581B2 (en) | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
-
2003
- 2003-08-22 JP JP2003299208A patent/JP4448673B2/ja not_active Expired - Fee Related
-
2004
- 2004-08-19 DE DE602004004708T patent/DE602004004708T2/de not_active Expired - Lifetime
- 2004-08-19 US US10/922,522 patent/US7582473B2/en not_active Expired - Fee Related
- 2004-08-19 EP EP04019716A patent/EP1508621B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1508621B1 (en) | 2007-02-14 |
| US20050042744A1 (en) | 2005-02-24 |
| DE602004004708D1 (de) | 2007-03-29 |
| DE602004004708T2 (de) | 2007-11-29 |
| JP2005065573A (ja) | 2005-03-17 |
| US7582473B2 (en) | 2009-09-01 |
| EP1508621A1 (en) | 2005-02-23 |
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