JP4411523B2 - 抗ウイルス剤 - Google Patents
抗ウイルス剤 Download PDFInfo
- Publication number
- JP4411523B2 JP4411523B2 JP2004061033A JP2004061033A JP4411523B2 JP 4411523 B2 JP4411523 B2 JP 4411523B2 JP 2004061033 A JP2004061033 A JP 2004061033A JP 2004061033 A JP2004061033 A JP 2004061033A JP 4411523 B2 JP4411523 B2 JP 4411523B2
- Authority
- JP
- Japan
- Prior art keywords
- antiviral agent
- virus
- cells
- algae
- elution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000003443 antiviral agent Substances 0.000 title claims description 24
- 239000000284 extract Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 241000195493 Cryptophyta Species 0.000 claims description 15
- 229920001282 polysaccharide Polymers 0.000 claims description 12
- 239000005017 polysaccharide Substances 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 11
- 241000700588 Human alphaherpesvirus 1 Species 0.000 claims description 10
- 241000725303 Human immunodeficiency virus Species 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 8
- 241000134090 Coccomyxa <Trebouxiophyceae> Species 0.000 claims description 5
- 230000000840 anti-viral effect Effects 0.000 claims description 5
- 239000004480 active ingredient Substances 0.000 claims description 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 4
- 239000012266 salt solution Substances 0.000 claims description 4
- 241000712461 unidentified influenza virus Species 0.000 claims description 4
- 241000195627 Chlamydomonadales Species 0.000 claims description 3
- 241000195642 Chlorococcaceae Species 0.000 claims description 3
- 241001464837 Viridiplantae Species 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims 3
- 241000007181 unidentified human coronavirus Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 29
- 230000000694 effects Effects 0.000 description 16
- 244000060011 Cocos nucifera Species 0.000 description 14
- 235000013162 Cocos nucifera Nutrition 0.000 description 14
- 241000700605 Viruses Species 0.000 description 13
- 230000002401 inhibitory effect Effects 0.000 description 9
- 150000004804 polysaccharides Chemical class 0.000 description 9
- 244000309467 Human Coronavirus Species 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000009385 viral infection Effects 0.000 description 6
- 241000712431 Influenza A virus Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 210000003501 vero cell Anatomy 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- 230000001093 anti-cancer Effects 0.000 description 3
- -1 boric acid ions Chemical class 0.000 description 3
- 230000007910 cell fusion Effects 0.000 description 3
- 230000000120 cytopathologic effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 150000002772 monosaccharides Chemical class 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- DTQVDTLACAAQTR-UHFFFAOYSA-N trifluoroacetic acid Substances OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
- 241001493065 dsRNA viruses Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229940124597 therapeutic agent Drugs 0.000 description 2
- 241001529453 unidentified herpesvirus Species 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000282552 Chlorocebus aethiops Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000002738 Giemsa staining Methods 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 231100000645 Reed–Muench method Toxicity 0.000 description 1
- 101800001690 Transmembrane protein gp41 Proteins 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 229920001284 acidic polysaccharide Polymers 0.000 description 1
- 150000004805 acidic polysaccharides Chemical class 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003602 anti-herpes Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000002648 combination therapy Methods 0.000 description 1
- 230000001609 comparable effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000009422 growth inhibiting effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001631 hypertensive effect Effects 0.000 description 1
- 230000005965 immune activity Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
コッコミクサの乾燥藻体(10g)にイオン交換水(200mL)を加え、還流下で1時間、加熱抽出を行った。抽出液を遠心分離(3000rpm、15min)し、上清を減圧濃縮しコッコミクサ藻体の抽出物(以下、CEという)70mLを得た。
CE-1〜CE-3の各画分を2N−トリフルオロ酢酸(TFA)に溶解し、121°Cで1時間、加水分解した。窒素ブローによりTFAを除去した後、濃縮した。これに、水素化ホウ素ナトリウム(NaBH4)を加え、生成した単糖類をアルジトールに還元し、過剰のNaBH4 を10%AcOH/MeOHに加え分解し、濃縮乾固した。さらに、MeOHを加え、濃縮乾固を5回行うことでホウ酸イオンを除去した。減圧下で乾燥後、無水酢酸を加え、100°Cで2時間、加熱することでアルジトールアセテートを得た。得られたアルジトールアセテートは、ガスクロマトグラフ/質量分析計により分析した。
HSV−1に対する活性は、アフリカミドリザル腎臓由来のVero細胞を宿主細胞として用いた。このVero細胞を48穴プレートに培養し、0.1PFU(プラーク形成単位)/細胞で感染後、この発明の抗ウイルス剤(CEおよびCE-1〜CE-3)の0.8〜500μg/mlの存在下で処理した。24時間後に収穫して、凍結、溶解処理を3回行った。この検体を適宜希釈して、35mmディッシュに別に培養したVero細胞に感染させ、翌日に染色後、プラークを計数した。無添加対照区のプラーク数を100%として、50%ウイルス増殖阻止濃度(IC50)を算出した。
IFVに対する活性は、イヌ腎臓由来のMDCK細胞を宿主細胞として用いた。このMDCK細胞を48穴プレートに培養し、0.1PFU/細胞で感染後、この発明の抗ウイルス剤(CEおよびCE-1〜CE-3)の0.8〜500μg/mlの存在下で処理した。以下、プラークアッセイはHSV−1と同様の方法で行った。
感染力のあるウイルスそのものは用いない細胞間融合アッセイを行った。すなわち、HIVの糖蛋白質であるgp160(gp120/gp41)を発現しているHeLa細胞(gp160+ HeLa細胞)と、宿主側のレセプターであるCD4を発現しているHeLa細胞(CD+ HeLa細胞)とを一定の割合で混合培養することによって、両者の細胞間で融合(多核巨細胞の形成)が起こることを利用して、この融合に対する阻止効果を検討した。この発明の抗ウイルス剤(CEおよびCE-1〜CE-3)の0.8−500μg/mlの存在下で24時間の混合培養を行った後、ギムザ染色し、顕微鏡下で多核巨細胞数を測定した。無添加対照区の巨細胞数を100%として、50%細胞融合阻止濃度(IC50)を算出した。
HCoVに対する活性は、ヒト胎児肺由来のMRC−5細胞を宿主細胞として用いた。このMRC−5細胞を48穴プレートに培養し、0.001TCID50(50%培養細胞感染量)/細胞で感染後、この発明の抗ウイルス剤(CEおよびCE-1〜CE-3)の0.8〜500μg/mlの存在下で処理した。3日後に収穫して、この検体を適宜希釈して、96穴プレートに別に用意したMRC−5細胞に感染させ、5日間培養する。細胞変性効果(CPE)の有無を判定して、Reed−Muench法によって、50%CPE阻止濃度(IC50)を算出した。
なお、以下に示す表の中で、A区はウイルス感染の時から収穫に至るまでの期間中にこの発明の抗ウイルス剤が存在することを、またB区はウイルス感染直後から収穫に至るまでの期間中にこの発明の抗ウイルス剤が存在することをそれぞれ意味する。
Claims (3)
- 緑色植物門(Chlorohta) 、緑藻綱(Chlorophyeae)、クロロコッカム目(Chlorococcales)、クロロコッカム科(Chlorococcaceae) に属するコッコミクサ・ミノール(Coccomyxa minor) 又はコッコミクサ・グロエオボトリディフォルミス(Coccomyxagloeobotrydiformis)としたコッコミクサ藻体の熱水抽出物から得られた多糖体画分を有効成分としてなり、単純ヘルペスウイルス1型、A型インフルエンザウイルス、ヒト免疫不全ウイルス、ヒトコロナウイルスに対する抗ウイルス活性を有することを特徴とする抗ウイルス剤。
- 前記多糖体画分が、前記コッコミクサ藻体の抽出物の蒸留水による溶出画分であることを特徴とする請求項1記載の抗ウイルス剤。
- 前記多糖体画分が、前記コッコミクサ藻体の抽出物の蒸留水による溶出後の無機塩溶液による溶出画分であることを特徴とする請求項1記載の抗ウイルス剤。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004061033A JP4411523B2 (ja) | 2004-03-04 | 2004-03-04 | 抗ウイルス剤 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004061033A JP4411523B2 (ja) | 2004-03-04 | 2004-03-04 | 抗ウイルス剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2005247757A JP2005247757A (ja) | 2005-09-15 |
JP4411523B2 true JP4411523B2 (ja) | 2010-02-10 |
Family
ID=35028593
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004061033A Expired - Fee Related JP4411523B2 (ja) | 2004-03-04 | 2004-03-04 | 抗ウイルス剤 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4411523B2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023085351A1 (ja) | 2021-11-11 | 2023-05-19 | 株式会社デンソー | T細胞の分化調節剤及び組成物 |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI461437B (zh) * | 2010-04-09 | 2014-11-21 | Univ Nat Taiwan | 快速萃取褐藻糖膠之方法 |
JP5597160B2 (ja) * | 2011-04-15 | 2014-10-01 | 株式会社日健総本社 | 抗ウイルス剤及びその製法 |
JP6125962B2 (ja) * | 2013-09-24 | 2017-05-10 | 株式会社デンソー | 抗ウイルス剤 |
JP2016065037A (ja) * | 2014-09-17 | 2016-04-28 | 株式会社日健総本社 | 抗ウイルス剤の製法及び該製法によって得られた抗ウイルス剤 |
EP4446337A2 (en) * | 2017-09-22 | 2024-10-16 | Centre National de la Recherche Scientifique | Mutated glycoprotein of vesicular stomatitis virus |
JP2020019729A (ja) * | 2018-07-31 | 2020-02-06 | 株式会社デンソー | ヘルペスウイルス回帰発症抑制剤 |
JP7233043B2 (ja) * | 2018-07-31 | 2023-03-06 | 株式会社デンソー | 抗インフルエンザ剤 |
WO2020026953A1 (ja) * | 2018-07-31 | 2020-02-06 | 株式会社デンソー | 抗ヘルペスウイルス剤 |
JP7378091B2 (ja) * | 2018-07-31 | 2023-11-13 | 株式会社デンソー | 殺ウイルス剤 |
GB2579600B (en) * | 2018-12-05 | 2023-07-05 | Byotrol Plc | Anti-viral composition |
-
2004
- 2004-03-04 JP JP2004061033A patent/JP4411523B2/ja not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023085351A1 (ja) | 2021-11-11 | 2023-05-19 | 株式会社デンソー | T細胞の分化調節剤及び組成物 |
Also Published As
Publication number | Publication date |
---|---|
JP2005247757A (ja) | 2005-09-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101724218B1 (ko) | 식물 마이크로 리보핵산의 추출, 제조 및 그 응용 | |
JP4411523B2 (ja) | 抗ウイルス剤 | |
RU2009101382A (ru) | Процесс получения поксвирусов и композиции поксвирусов | |
KR20140039157A (ko) | 항미생물성 융합 화합물 및 그의 용도 | |
CN1312722A (zh) | 得自鼠尾草属的具有抗病毒活性的化合物 | |
CA2757895A1 (en) | Method for purifying the rabies virus | |
VA et al. | Antiviral activity of total polysaccharide fraction of water and ethanol extracts of Pleurotus pulmonarius against the influenza A virus | |
JP6185093B2 (ja) | カワラタケ抽出物、その調製方法および使用 | |
AU629855B2 (en) | Therapeutic agent for aids and process for preparing it | |
CN111253498A (zh) | 一种褐藻多糖衍生物纳米胶束的制备 | |
CN110279752B (zh) | 速生桉叶提取物及其制备方法和抗hiv应用 | |
JP2020532588A (ja) | シアノバクテリア抽出物、その調製法と利用法 | |
CN1822847A (zh) | 冬虫夏草菌丝体抽提物的分离物及经口摄取用组合物 | |
CN112957388B (zh) | 甘蓝型油菜-菘蓝e单体附加系在抑制流感病毒中的应用 | |
WO2011029170A1 (en) | Process to obtain a homeopathic medicament and use thereof | |
JPH0466536A (ja) | 抗ウィルス物質とその製造方法 | |
CN114099599A (zh) | 水蜈蚣草和β-石竹烯的组合物在防治病毒感染中的应用 | |
CN1206717A (zh) | β-D-(1-4)-葡聚糖硫酸酯化合物 | |
Abderrahman | Mitodepressive effect of Rubia cordifolia extract on the bone marrow cells of mice | |
JPH0567611B2 (ja) | ||
CN109419939A (zh) | 一种用于治疗手足口病的药物组合物 | |
CN1683413A (zh) | 基因工程法制备抗乙肝病毒特异性转移因子的方法 | |
CN114099600A (zh) | 水蜈蚣草在预防和治疗病毒感染中的应用 | |
CN116173102A (zh) | 香椿花提取物在制备抗热应激的药物中的应用 | |
CN116270803A (zh) | 香椿花提取物在制备抗氧化的药物中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20051207 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090710 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090903 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20091015 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20091104 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121127 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 4411523 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121127 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20131127 Year of fee payment: 4 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |