JP4399164B2 - 核酸の磁気単離および精製の方法 - Google Patents

核酸の磁気単離および精製の方法 Download PDF

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JP4399164B2
JP4399164B2 JP2002566667A JP2002566667A JP4399164B2 JP 4399164 B2 JP4399164 B2 JP 4399164B2 JP 2002566667 A JP2002566667 A JP 2002566667A JP 2002566667 A JP2002566667 A JP 2002566667A JP 4399164 B2 JP4399164 B2 JP 4399164B2
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nucleic acid
concentration
magnetizable cellulose
magnetizable
dna
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JP2004523238A (ja
JP2004523238A5 (enExample
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アール． ディー． ナージェッシ
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プロメガ・コーポレーション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2002566667A 2001-02-16 2001-10-05 核酸の磁気単離および精製の方法 Expired - Lifetime JP4399164B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26972901P 2001-02-16 2001-02-16
PCT/US2001/031637 WO2002066993A1 (en) 2001-02-16 2001-10-05 Magnetic isolation and purification of nucleic acids

Publications (3)

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JP2004523238A JP2004523238A (ja) 2004-08-05
JP2004523238A5 JP2004523238A5 (enExample) 2006-01-05
JP4399164B2 true JP4399164B2 (ja) 2010-01-13

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JP2002566667A Expired - Lifetime JP4399164B2 (ja) 2001-02-16 2001-10-05 核酸の磁気単離および精製の方法

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EP (3) EP2090655B1 (enExample)
JP (1) JP4399164B2 (enExample)
AT (1) ATE422542T1 (enExample)
AU (1) AU2001296762B2 (enExample)
CA (1) CA2438066A1 (enExample)
DE (1) DE60137652D1 (enExample)
ES (1) ES2322841T3 (enExample)
WO (1) WO2002066993A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6855499B1 (en) 2001-02-16 2005-02-15 Cortex Biochem, Inc. Magnetic isolation and purification of nucleic acids
DK1442045T3 (da) * 2001-11-06 2009-02-09 Cortex Biochem Inc Isolering og oprensning af nukleinsyrer
JP3983125B2 (ja) * 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
EP1510577A1 (en) * 2003-08-29 2005-03-02 Qiagen GmbH Method for magnetic bead isolation of nucleic acids
EP1664295A4 (en) * 2003-09-09 2007-12-05 Fujifilm Corp PROCESS FOR INSULATING AND PURIFYING NUCLEIC ACID
JP4284250B2 (ja) * 2003-09-09 2009-06-24 富士フイルム株式会社 核酸分離精製方法
US20050106602A1 (en) 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US8426126B2 (en) * 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
DE102007009347B4 (de) 2007-02-27 2009-11-26 Agowa Gmbh Verfahren zur Isolierung von Nukleinsäuren
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
TWI394839B (zh) 2008-10-27 2013-05-01 Qiagen Gaithersburg Inc 快速結果雜交捕捉法及系統
JP5738278B2 (ja) 2009-05-01 2015-06-24 キアジェン ゲイサーズバーグ インコーポレイテッド 試料中のｒｎａスプライシング形態を検出するための非標的増幅法
AU2010291990B2 (en) * 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
EP2528932B1 (en) 2010-01-29 2016-11-30 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
EP2576840B1 (en) 2010-05-25 2018-10-17 QIAGEN Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
EP3447141B1 (en) 2013-03-18 2020-08-05 PreAnalytiX GmbH Stabilisation of biological samples
JP6736463B2 (ja) 2013-12-02 2020-08-05 バイオカーティス エヌ ヴイ 循環核酸の抽出
EP3218480A1 (en) 2014-11-14 2017-09-20 Corning Incorporated Methods and kits for post-ivt rna purification
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8507706D0 (en) * 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
EP0411944B1 (en) * 1989-08-04 1998-06-10 BEHRINGWERKE Aktiengesellschaft Heterogeneous binding assays
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
ES2322841T3 (es) 2009-06-30
EP2090655B1 (en) 2013-05-08
ATE422542T1 (de) 2009-02-15
EP2090655A1 (en) 2009-08-19
AU2001296762B2 (en) 2007-03-15
WO2002066993A1 (en) 2002-08-29
EP1368629A4 (en) 2005-07-06
EP2363476A1 (en) 2011-09-07
JP2004523238A (ja) 2004-08-05
EP1368629B1 (en) 2009-02-11
DE60137652D1 (de) 2009-03-26
EP1368629A1 (en) 2003-12-10
EP2363476B1 (en) 2013-05-29
CA2438066A1 (en) 2002-08-29

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