ATE422542T1 - Magnetische isolierung und reinigung von nukleinsäuren - Google Patents
Magnetische isolierung und reinigung von nukleinsäurenInfo
- Publication number
- ATE422542T1 ATE422542T1 AT01977661T AT01977661T ATE422542T1 AT E422542 T1 ATE422542 T1 AT E422542T1 AT 01977661 T AT01977661 T AT 01977661T AT 01977661 T AT01977661 T AT 01977661T AT E422542 T1 ATE422542 T1 AT E422542T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- purification
- derivatives
- magnetic isolation
- magnetizable cellulose
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 229920002678 cellulose Polymers 0.000 abstract 3
- 239000001913 cellulose Substances 0.000 abstract 3
- 239000002245 particle Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 229920001515 polyalkylene glycol Polymers 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26972901P | 2001-02-16 | 2001-02-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE422542T1 true ATE422542T1 (de) | 2009-02-15 |
Family
ID=23028437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01977661T ATE422542T1 (de) | 2001-02-16 | 2001-10-05 | Magnetische isolierung und reinigung von nukleinsäuren |
Country Status (8)
| Country | Link |
|---|---|
| EP (3) | EP2090655B1 (enExample) |
| JP (1) | JP4399164B2 (enExample) |
| AT (1) | ATE422542T1 (enExample) |
| AU (1) | AU2001296762B2 (enExample) |
| CA (1) | CA2438066A1 (enExample) |
| DE (1) | DE60137652D1 (enExample) |
| ES (1) | ES2322841T3 (enExample) |
| WO (1) | WO2002066993A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US6855499B1 (en) | 2001-02-16 | 2005-02-15 | Cortex Biochem, Inc. | Magnetic isolation and purification of nucleic acids |
| DK1442045T3 (da) * | 2001-11-06 | 2009-02-09 | Cortex Biochem Inc | Isolering og oprensning af nukleinsyrer |
| JP3983125B2 (ja) * | 2002-07-19 | 2007-09-26 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| EP1510577A1 (en) * | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| EP1664295A4 (en) * | 2003-09-09 | 2007-12-05 | Fujifilm Corp | PROCESS FOR INSULATING AND PURIFYING NUCLEIC ACID |
| JP4284250B2 (ja) * | 2003-09-09 | 2009-06-24 | 富士フイルム株式会社 | 核酸分離精製方法 |
| US20050106602A1 (en) | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Simplified methods for isolating nucleic acids from cellular materials |
| US8426126B2 (en) * | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| DE102007009347B4 (de) | 2007-02-27 | 2009-11-26 | Agowa Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| EP2157181A1 (de) | 2008-08-13 | 2010-02-24 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Verfahren zur Isolation von Nukleinsäuren und Testkit |
| TWI394839B (zh) | 2008-10-27 | 2013-05-01 | Qiagen Gaithersburg Inc | 快速結果雜交捕捉法及系統 |
| JP5738278B2 (ja) | 2009-05-01 | 2015-06-24 | キアジェン ゲイサーズバーグ インコーポレイテッド | 試料中のｒｎａスプライシング形態を検出するための非標的増幅法 |
| AU2010291990B2 (en) * | 2009-09-14 | 2016-05-05 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| US9605303B2 (en) | 2010-01-29 | 2017-03-28 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an HPV in a sample |
| EP2528932B1 (en) | 2010-01-29 | 2016-11-30 | QIAGEN Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| CA2799200A1 (en) | 2010-05-19 | 2011-11-24 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| EP2576840B1 (en) | 2010-05-25 | 2018-10-17 | QIAGEN Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically-truncated probes |
| US9885092B2 (en) | 2011-02-24 | 2018-02-06 | Qiagen Gaithersburg Inc. | Materials and methods for detection of HPV nucleic acids |
| DE102012012523B4 (de) | 2012-06-26 | 2015-02-12 | Magnamedics Gmbh | Reinigung von Nukleinsäuren |
| EP3447141B1 (en) | 2013-03-18 | 2020-08-05 | PreAnalytiX GmbH | Stabilisation of biological samples |
| JP6736463B2 (ja) | 2013-12-02 | 2020-08-05 | バイオカーティス エヌ ヴイ | 循環核酸の抽出 |
| EP3218480A1 (en) | 2014-11-14 | 2017-09-20 | Corning Incorporated | Methods and kits for post-ivt rna purification |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8507706D0 (en) * | 1985-03-25 | 1985-05-01 | Genetics Int Inc | Magnetic nucleic acid sequences |
| US4921805A (en) * | 1987-07-29 | 1990-05-01 | Life Technologies, Inc. | Nucleic acid capture method |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| EP0411944B1 (en) * | 1989-08-04 | 1998-06-10 | BEHRINGWERKE Aktiengesellschaft | Heterogeneous binding assays |
| GB9003253D0 (en) | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
2001
- 2001-10-05 WO PCT/US2001/031637 patent/WO2002066993A1/en not_active Ceased
- 2001-10-05 JP JP2002566667A patent/JP4399164B2/ja not_active Expired - Lifetime
- 2001-10-05 EP EP08021881.1A patent/EP2090655B1/en not_active Expired - Lifetime
- 2001-10-05 CA CA002438066A patent/CA2438066A1/en not_active Abandoned
- 2001-10-05 AU AU2001296762A patent/AU2001296762B2/en not_active Ceased
- 2001-10-05 EP EP10176947.9A patent/EP2363476B1/en not_active Expired - Lifetime
- 2001-10-05 ES ES01977661T patent/ES2322841T3/es not_active Expired - Lifetime
- 2001-10-05 AT AT01977661T patent/ATE422542T1/de not_active IP Right Cessation
- 2001-10-05 EP EP01977661A patent/EP1368629B1/en not_active Expired - Lifetime
- 2001-10-05 DE DE60137652T patent/DE60137652D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2322841T3 (es) | 2009-06-30 |
| JP4399164B2 (ja) | 2010-01-13 |
| EP2090655B1 (en) | 2013-05-08 |
| EP2090655A1 (en) | 2009-08-19 |
| AU2001296762B2 (en) | 2007-03-15 |
| WO2002066993A1 (en) | 2002-08-29 |
| EP1368629A4 (en) | 2005-07-06 |
| EP2363476A1 (en) | 2011-09-07 |
| JP2004523238A (ja) | 2004-08-05 |
| EP1368629B1 (en) | 2009-02-11 |
| DE60137652D1 (de) | 2009-03-26 |
| EP1368629A1 (en) | 2003-12-10 |
| EP2363476B1 (en) | 2013-05-29 |
| CA2438066A1 (en) | 2002-08-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1368629 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |