JP4266817B2 - 酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途 - Google Patents

酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途 Download PDF

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JP4266817B2
JP4266817B2 JP2003510787A JP2003510787A JP4266817B2 JP 4266817 B2 JP4266817 B2 JP 4266817B2 JP 2003510787 A JP2003510787 A JP 2003510787A JP 2003510787 A JP2003510787 A JP 2003510787A JP 4266817 B2 JP4266817 B2 JP 4266817B2
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collagenase
type
chromatographic
chromatography
elution
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JP2004535197A5 (https=
JP2004535197A (ja
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クアフュルスト・マンフレート
シュミッドバウアー・シュテファン
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ノルドマルク・アルツナイミッテル・ゲゼルシヤフト・ミト・ベシュレンクテル・ハフツング・ウント・コンパニー・コマンデイトゲゼルシヤフト
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22008—Clostripain (3.4.22.8)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2003510787A 2001-07-02 2002-06-01 酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途 Expired - Fee Related JP4266817B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10131994 2001-07-02
DE10134347A DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms
PCT/EP2002/006021 WO2003004628A2 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms

Publications (3)

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JP2004535197A JP2004535197A (ja) 2004-11-25
JP2004535197A5 JP2004535197A5 (https=) 2008-05-08
JP4266817B2 true JP4266817B2 (ja) 2009-05-20

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JP2003510787A Expired - Fee Related JP4266817B2 (ja) 2001-07-02 2002-06-01 酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途

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US (1) US7083964B2 (https=)
EP (1) EP1402008B1 (https=)
JP (1) JP4266817B2 (https=)
CN (1) CN1308442C (https=)
AT (1) ATE302844T1 (https=)
AU (1) AU2002354696A1 (https=)
CA (1) CA2447818C (https=)
WO (1) WO2003004628A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622562B2 (en) 2002-06-26 2009-11-24 Zimmer Orthobiologics, Inc. Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
DE10331171A1 (de) * 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
US7824711B2 (en) 2003-12-11 2010-11-02 Isto Technologies, Inc. Particulate cartilage system
JP5292533B2 (ja) 2005-08-26 2013-09-18 ジンマー・インコーポレイテッド インプラントおよび関節疾患の治療、置換および治療方法
US20070181479A1 (en) * 2005-11-21 2007-08-09 Pentax Corporation Column and method of manufacturing the column
US7811560B2 (en) * 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
AU2008240191B2 (en) 2007-04-12 2013-09-19 Zimmer, Inc. Compositions and methods for tissue repair
US10071143B1 (en) 2007-05-03 2018-09-11 The Research Foundation For The State University Of New York Methods for non-surgical treatment of carpal tunnel syndrome
US20100159564A1 (en) * 2007-11-30 2010-06-24 Dwulet Francis E Protease resistant recombinant bacterial collagenases
US8853261B2 (en) * 2008-01-21 2014-10-07 Laila Nutraceuticals Nutraceutical composition from Garcinia mangostana
ES2388219T3 (es) 2008-06-02 2012-10-10 F. Hoffmann-La Roche Ag Purificación mejorada de colagenasas a partir de un cultivo líquido de Clostridium histolyticum
PT2802652T (pt) 2012-01-12 2019-09-10 Endo Global Ventures Enzimas de clostridium histolyticum
US9636385B2 (en) 2012-10-24 2017-05-02 The Research Foundation For The State University Of New York Use of collagenase to treat glaucoma
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
GB201507207D0 (en) * 2015-04-24 2015-06-10 Givaudan Sa Enzymes and applications thereof
EP4591945A3 (en) 2017-03-01 2026-04-15 Endo Operations Limited Apparatus and method for assessing and treating cellulite
JP7227918B2 (ja) 2017-03-28 2023-02-22 エンド ベンチャーズ リミテッド コラゲナーゼ産生の改良された方法
CN112996801A (zh) * 2018-11-26 2021-06-18 北卡罗来纳州大学 用于捕获宿主细胞蛋白的肽配体
EP3924363A1 (de) * 2019-02-14 2021-12-22 Nordmark Pharma GmbH Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332503A (en) 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
DE4445891A1 (de) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Rekombinante Proteinase aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden

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Publication number Publication date
ATE302844T1 (de) 2005-09-15
CA2447818C (en) 2011-01-04
US7083964B2 (en) 2006-08-01
CN1308442C (zh) 2007-04-04
JP2004535197A (ja) 2004-11-25
CN1529751A (zh) 2004-09-15
CA2447818A1 (en) 2003-01-16
US20040137596A1 (en) 2004-07-15
WO2003004628A3 (de) 2003-09-18
EP1402008B1 (de) 2005-08-24
WO2003004628A2 (de) 2003-01-16
AU2002354696A1 (en) 2003-01-21
EP1402008A2 (de) 2004-03-31

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