JP4204503B2 - Hair growth / hair nourishing composition for oral intake and food and drink containing the composition - Google Patents

Description

  The present invention relates to a hair-restoring / hair-restoring composition that contains N-acetylglucosamine and a collagen peptide as active ingredients and can be expected to have a hair-restoring / hair-restoring effect when taken orally, and to a food or drink containing the composition.

  In modern society, people are often exposed to various stresses, and lifestyle habits such as eating habits tend to be irregular, and accordingly, anxiety about hair such as thinning hair and hair loss also increases. Yes. This kind of anxiety about hair has been centered on middle-aged and older men, but due to changes in the social environment in recent years, the scope has spread to young people and women. Is getting higher.

  Therefore, development of a hair restorer, a hair nourishing agent, etc. which have a higher effect is performed actively. For example, many external hair restorers and nourishing agents currently used use hormones, vitamins, amino acids, vasodilators, anti-inflammatory agents, and the like as active ingredients.

  On the other hand, as a hair growth agent and a hair nourishing agent for internal use, for example, in Patent Document 1 below, one or more plant extracts selected from enamel, ginseng, hypericum, salvia, bodaiju, rakanka, kombu, hijiki, hibamata, wakame It is disclosed that the food and drink for hair growth can be further containing one or more protein degradation products selected from silk protein, keratin protein, and collagen protein. Is described.

  Patent Document 2 listed below contains soybean saponin as an essential component, a collagen protein hydrolyzate having a molecular weight of 200 to 10,000, a keratin protein hydrolyzate having a molecular weight of 200 to 10,000, and a sulfur-containing amino acid. A hair growth nourishing food characterized in that it contains one or more selected from the group consisting of:

  Patent Document 3 below discloses a nutritional supplement for hair growth and / or hair growth characterized by containing at least one yeast or yeast extract, at least one vitamin and at least one mineral. It is disclosed.

  Patent Document 4 below discloses a hair restorer for oral consumption characterized by containing royal jelly as an active ingredient.

  Patent Document 5 below discloses a food composition characterized by containing millet, ginkgo biloba extract and isoflavone, and effectively improves thinning and hair loss by ingesting the composition. The effect is demonstrated.

Patent Document 6 below discloses a hair nourishing agent characterized by containing at least one of a hyaluronidase activity inhibitory component and a hyaluronic acid synthesis promoting component. And as the hyaluronidase activity inhibitory component, cromoglycic acid, tranilast, amlexanox, repirinast, pemirolast, ketotifen, azelastine, mequitazine, oxatomide, epinastine, emedastine, labdocin, and salts thereof, and inter-α trypsin inhibitor and The degradation fragment is illustrated. Examples of the hyaluronic acid synthesis promoting component include N-acetylglucosamine, N-acetylgalactosamine, D-gluconic acid, D-glucuronic acid, and D-galacturonic acid. Further, it is described that the hair nurturing agent can be used for uses such as nutritional drinks and foods.
JP 2001-321121 A Japanese Patent No. 3407004 JP 2001-142 A JP-A-8-104644 Japanese Patent Laid-Open No. 2001-238637 JP 2003-81778 A

  However, conventional external hair restorers and hair nourishing agents have sufficient hair-growth / hair-restoration effects due to safety concerns such as skin irritation and the fact that there are limits to the amount of intake from the skin even though they are effective ingredients. Was not obtained, and the effect was never satisfactory.

  In addition, there were still few internal hair restorers and hair nourishing agents on the market because of their insufficient effects.

  Accordingly, an object of the present invention is to provide a hair-restoring / hair-restoring composition for oral intake that can be safely and easily ingested and can be expected to have a sufficient hair-restoring / hair-restoring effect, and a food and drink containing the composition. There is to do.

As a result of intensive studies to solve the above problems, the present inventors have found that chitinous substances widely distributed in nature as polysaccharides contained in crustaceans such as crabs and shrimps, insects, fungi and the like. It has been found that an excellent hair-growth / hair-growth effect can be obtained by using N-acetylglucosamine, which is a constituent sugar, and a collagen peptide derived from fish , and the present invention has been completed.

That is, the hair-restoring / hair-restoring composition for oral intake of the present invention is a composition used for hair- restoration or hair-restoration, and contains N-acetylglucosamine and a fish-derived collagen peptide as active ingredients. It is characterized by.

The hair-restoring / hair-restoring composition for oral consumption of the present invention is expected to have excellent hair-restoring / hair-restoring effect due to the synergistic effect of these components by containing N-acetylglucosamine and fish-derived collagen peptide as active ingredients. it can.

In the hair growth / hair nourishing composition for oral intake of the present invention, the fish-derived collagen peptide preferably has an average molecular weight of 1,000 to 10,000.

  According to this aspect, it is possible to provide a hair-restoring / hair-restoring composition for oral intake that can be blended in various foods and drinks with low viscosity.

Further, collagen peptide derived from the fish, Yu away amino acid content of 1% or less, preferably not more than arsenic content 2 ppm.

  According to this aspect, it is possible to provide a highly safe hair-restoring / hair-restoring composition for oral intake that has no fish-specific taste and smell.

Furthermore, chitin oligosaccharides, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract, and minerals selected from magnesium, sodium, calcium, potassium and zinc derived from deep sea water It is preferable to contain one or more selected.

According to this aspect, it is possible to provide a hair-restoring / hair-restoring composition for oral intake that can be expected to have a better hair-restoring / hair-restoration effect due to the synergistic effect of each of the above components, N-acetylglucosamine and a collagen peptide derived from fish. .

According to the present invention, by containing N-acetylglucosamine and a fish-derived collagen peptide as active ingredients, it can be ingested safely and easily, and can be expected to have an excellent preventive effect on thinning hair, hair loss, etc. A hair growth / hair nourishing composition can be provided.

  The origin of N-acetylglucosamine used in the present invention is not particularly limited, but for example, chitin obtained from crustaceans such as crabs and shrimps is used as a raw material, and Japanese Patent Publication No. 5-33037 and Japanese Patent Application Laid-Open No. 2000-281696. Can be obtained according to the method described in the above.

  In other words, N-acetylchitooligosaccharide-containing mixture obtained by partially hydrolyzing polysaccharide chitin prepared from crustacean shells such as crabs and shrimps with an acid can be hydrolyzed with respect to N-acetylchitooligosaccharide. It can be obtained by a method of decomposing it by the action of an enzyme (eg, lysotium, chitinase, chitobiase, etc.) having The N-acetylglucosamine thus obtained is a natural type that has not been chemically synthesized, and therefore can be safely ingested as a food. In addition, the natural type N-acetylglucosamine manufactured as mentioned above is marketed, for example, a brand name "Marine Sweet" (made by Yaizu Suisan Chemical Co., Ltd.) etc. can be used.

Next, the fish-derived collagen peptide used in the present invention is a peptide obtained by treating fish-derived collagen with a proteolytic enzyme or the like, and has an average molecular weight (number average molecular weight) of 1,000 to 10,000. Are preferably used. The origin of the raw material (collagen) is due to the influence of recent mad cow disease, swine foot-and-mouth disease, avian influenza, etc. Collagen .

  In the present invention, fish-derived collagen peptides having a free amino acid content of 1% by mass or less and an arsenic content of 2 ppm or less are preferably used. Such a collagen peptide can be obtained according to the method described in JP-A-2003-238597. That is, for example, skipjack, tuna, marlin, cod, horse mackerel, mackerel, salmon, trout, saury, eel, tilapia, riverfish, grouper, halibut, flounder, flounder, herring, sardine, tilapia, shark, ray, puffer, yellowtail, Collagen derived from fish is extracted by adding water to fish skin and / or fish bones obtained from scorpionfish, rockfish and the like, followed by heating extraction or pressure heating extraction.

  The above extract containing collagen is treated with a protein hydrolase to peptideize collagen. The protein hydrolase is not particularly limited, and neutral protease, alkaline protease, acidic protease, or enzyme preparation containing them can be used.

The enzyme degradation product obtained in the above step is treated with a reverse osmosis membrane to recover the concentrated solution. As the reverse osmosis membrane, one having a salt rejection of 10 to 50% is preferably used. Examples of such reverse osmosis membranes include trade name “NTR-7410”, trade name “NTR-7430”, trade name “NTR-7450” (all manufactured by Nitto Denko). In addition, it is preferable to add 1 to 10 times the amount of the original solution, preferably 3 to 5 times the amount of water while adding water appropriately during the concentration to allow the solution to permeate. By repeating the hydration operation, impurities can be removed more efficiently, and the fish-specific flavor can be reduced and a safer collagen peptide can be obtained. The concentrated solution (collagen peptide) obtained as described above may be used as it is, or may be used after appropriately drying and pulverizing. Fish-derived collagen peptides are commercially available from various companies. For example, the trade name “Marine Matrix” (manufactured by Yaizu Suisan Chemical Co., Ltd.) can be used.

The blending amount of N-acetylglucosamine and the collagen peptide derived from fish in the hair growth / hair nourishing composition for oral intake of the present invention depends on the formulation form of the composition, but is 0.1-80% by mass of N-acetylglucosamine. In addition, 0.1 to 80% by mass of a fish-derived collagen peptide is preferable, 1 to 50% by mass of N-acetylglucosamine, and 1 to 50% by mass of a collagen peptide derived from fish .

The hair-restoring / hair-restoring composition for oral intake of the present invention is derived from chitin oligosaccharides, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract, and deep sea water as other active ingredients. It is preferable to contain one or more selected from minerals. By using these components in combination, a more excellent hair growth / hair restoration effect can be expected due to a synergistic effect with N-acetylglucosamine and a collagen peptide derived from fish .

  For example, chitin oligosaccharides can be obtained by chemically or biochemically treating chitin prepared from shellfish shells such as crabs and shrimps by conventional methods. That is, it can be obtained by partially hydrolyzing chitin with an acid or an enzyme, and fractionating and purifying the desired degree of polymerization from the decomposed product by a method such as column chromatography or solvent fractionation if necessary. . The degree of polymerization of chitin oligosaccharides is usually about 2-7 sugars, that is, N-acetylchitobiose, N-acetylchitotriose, N-acetylchitotetraose, N-acetylchitopentaose, N-acetylchito Hexaose and N-acetylchitoheptaose are preferable, and one or a mixture of two or more thereof is preferably used. Chitin oligosaccharides or mixtures thereof are commercially available from various companies, and for example, “NA-COS-Y” (trade name, manufactured by Yaizu Suisan Chemical Co., Ltd.) can be used.

  The shark cartilage extract contains sodium chondroitin sulfate, which is a polysaccharide having a molecular weight of about 100,000 to several hundred thousand, as a main component, and is obtained from a shark cartilage raw material such as shark fin according to a known method. Can do. In the present invention, those obtained according to the method described in JP-A No. 2002-281935 are preferably used. That is, after the shark cartilage raw material is washed with water, the shark cartilage extract is extracted by a method of extracting from the shark cartilage raw material with an alkaline solution, a method using a proteolytic enzyme, a method of extracting with hot water, or the like. The obtained shark cartilage extract is subjected to enzymatic degradation as it is or using a protein hydrolase, and then decolorized and deodorized using a solid adsorbent such as activated carbon, alumina, silica gel, activated clay, and the like. The shark cartilage extract decolorized and deodorized or the enzyme degradation solution thereof is preferably treated with a separation membrane using an NF membrane having a salt rejection of 10 to 60%, so that the arsenic content is low and there is no off-flavor or off-flavor. Shark cartilage extract can be obtained. Shark cartilage extract is commercially available from various companies, for example, trade name “Malinker Tridge P” (manufactured by Yaizu Suisan Chemical Co., Ltd.), trade name “Malinker Tridge 40” (manufactured by Yaizu Suisan Chemical Co., Ltd.) ) Etc. can be used.

  Fucoidan is a kind of sulfated polysaccharide (sulfated fucose polymer) contained in brown algae such as mozuku, kombu, seaweed, and hijiki, and can be obtained from the raw material seaweed according to a known method. That is, an aqueous solution of an inorganic acid (for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, etc.) or an organic acid (for example, acetic acid, citric acid, oxalic acid, succinic acid, formic acid, propionic acid, etc.) 5, The raw material density | concentration is added so that it may become 0.1-3 mass% as a dried material, and the method of extracting by stirring for 0 to 24 hours at 10-100 degreeC is mentioned. Preferably, hydrochloric acid is added to the above raw material so that the pH is 2 to 3 and the raw material concentration is 2 to 3% by mass as a dried product, and extracted at 20 to 30 ° C. for 15 to 20 hours. More preferably, the extraction is performed after the raw material is pulverized with a mixer or the like. The extract thus obtained is filtered, neutralized to pH 7-8 with an alkali agent, and then concentrated under reduced pressure until the solid content concentration is 1-30% by mass. Crude fucoidan can be obtained by adding 2 to 4 times by mass of ethanol to the concentrate and refrigeration and standing for 0 to 24 hours, and then collecting and drying the precipitate by centrifugation. The crude fucoidan obtained as described above may be purified as necessary. As a purification method, after dissolving the above-mentioned crude fucoidan in 30 to 100 mass times demineralized water, cetylpyridinium chloride is added so as to be 1 to 6 mass%, preferably 2 to 5 mass%, and 10 to 10 at room temperature. After stirring for 20 hours, the precipitate is collected by centrifugation. This precipitate is dissolved in 2 to 60 mass times of 4M calcium chloride solution, insoluble matter is removed by filtration, and 2 to 3 mass times of ethanol is added and stirred, and then at 4 to 25 ° C. for 2 to 8 hours. The purified fucoidan can be obtained by allowing to stand and centrifuging to collect and dry the precipitate (see Agricultural and Biological Chemistry Vol. 30, No. 5, p. 495-499, 1966). Fucoidan is commercially available from various companies, and for example, the trade name “Fucoidan YSK” (manufactured by Yaizu Suisan Chemical Co., Ltd.) can be used.

  Anserine is a kind of imidazole compound, and can be obtained by purifying an extract extracted from fish meat, chicken meat, livestock meat or the like by a method such as water extraction, hot water extraction, alcohol extraction, or supercritical extraction. That is, an extract is prepared from meat such as skipjack, tuna, cow, chicken, etc. according to a conventional method, and water is added as appropriate to adjust the Brix (Bx.) Of the extract to 1 to 10%. The high molecular weight protein is removed using a molecular weight of 5,000 to 50,000), and the low molecular weight peptide fraction is recovered. Then, according to the method of the literature (Suyama et al: Bull. Japan. Soc. Scient. Fish., 33, 141-146, 1967), the low-molecular peptide fraction appropriately concentrated was subjected to ion exchange chromatography using a strongly acidic resin. Apply to the chromatography and collect the eluate. Then, the eluate can be obtained by desalting, adjusting the pH, and drying it by freeze drying or the like (see JP 2002-338473 A, JP 2002-173442 A, etc.). Anserine is commercially available from various companies, and for example, the trade name “Marine Active” (manufactured by Yaizu Suisan Chemical Co., Ltd.) can be used.

  Simnol sulfate is a component of deep sea shark bile, and can be extracted from the liver or gallbladder of deep sea sharks according to the method described in JP-T-2-503554. Simnol sulfate is commercially available from various companies. For example, trade name “Shimnol Sulfate” (manufactured by Yaizu Suisan Chemical Co., Ltd.) can be used.

  The yeast extract can be obtained according to a known method using, for example, beer yeast, wine yeast, baker's yeast, sake yeast, or the like as a raw material. Yeast extracts are commercially available from various companies. For example, the trade name “Alo-Mild” (manufactured by Kojin Co., Ltd.), the trade name “marine yeast extract powder” (manufactured by Meio Bussan Co., Ltd.), etc. may be used. it can.

  In addition, seaweed extracts include dehydrated algae (for example, mozuku, kombu, wakame, hijiki, kajime, arame, etc.), red algae (for example, laver, proboscis, mafonori, tsunomata, ogonori, egonori, etc.), green algae (for example, aonori) Extract seaweed that can be used for food, such as water extract, hot water extraction, alcohol extraction, or supercritical extraction, and concentrate and powder as appropriate. Can be obtained. As the seaweed extract, a commercially available seaweed extract may be used. For example, the trade name “Konbu extract F No. 500” (Yaizu Suisan Chemical Co., Ltd.), the trade name “Konbu extract powder YK” (Yaizu) Suisan Chemical Co., Ltd.) can be used.

  Further, deep seawater-derived minerals are obtained by appropriately concentrating and dry-powdering a mineral fraction obtained by removing only sodium chloride from seawater collected from a depth of 200 m or more by a desalting operation such as electrodialysis. be able to. The mineral derived from deep sea water thus obtained contains magnesium, sodium, calcium, potassium, zinc and the like.

  Although the compounding quantity of each said component in the hair growth and hair nourishing composition for oral intake of this invention can be suitably set with the formulation form of a composition, 0.1-50 mass% is preferable normally, 0.5-30 mass% Is more preferable.

The hair growth / hair nourishing composition for oral consumption of the present invention comprises N-acetylglucosamine and a collagen peptide derived from fish , and chitin oligosaccharide, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract as appropriate , And minerals derived from deep ocean water. Moreover, an excipient | filler, various vitamins, saccharides, a fragrance | flavor, etc. can also be mix | blended as needed.

  The formulation form of the hair-restoring / hair-restoring composition for oral intake of the present invention is not particularly limited, and can be in the form of a solution, powder, granule, tablet, capsule or the like.

The effective intake of the hair-restoring / hair-restoring composition for oral intake of the present invention is preferably 100 to 15,000 mg / day in terms of N-acetylglucosamine, and preferably 100 to 20,000 mg / day in terms of fish-derived collagen peptide. -More preferably 500 to 1,500 mg / day in terms of acetylglucosamine and 500 to 5,000 mg / day in terms of collagen peptide. Moreover, when using together chitin oligosaccharide, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract, minerals derived from deep sea water, etc., about these components, it is 100-10,000 mg. / Day is preferable, and 100 to 1,000 mg / day is more preferable.

  The hair-restoring / hair-restoring composition for oral intake of the present invention may be ingested as it is, but can also be blended in various foods and drinks. There are no particular restrictions on the type and form of the food and drink, for example, beverages, baked goods, frozen confectionery, tablet confectionery, candy, gummy, jelly, caramel, jam, chocolate, gum, Japanese confectionery, soups, bread, various retort foods, Examples include sugar beet, seafood products, ham, sausages, seasonings, powdered, liquid, and granular supplements. Also, the blending method is not particularly limited, and may be mixed together with other raw materials according to the method for producing each food and drink.

  The amount of the hair-restoring / hair-restoring composition for oral intake of the present invention in the food or drink can be appropriately set based on the effective intake, but usually 0.1 to 100 parts by weight of the food or drink. 50 mass parts is preferable and 1-25 mass parts is more preferable.

<Example 1>
Each raw material was blended according to the blending shown in Table 1, and A to I (Examples), J (Control Examples), and K to L (Comparative Examples) round tablets (250 mg / tablet) were produced using a tableting machine. . This tablet was excellent in hardness, disintegration, and tableting property.

  Next, the hair growth / hair restoration effect was evaluated by the following method using each tablet. In other words, volunteer adults (27 to 52 years old) who suffer from hair loss and thinning hair are divided into groups of 10 persons (5 males and 5 females), and 10 tablets / day are taken for 2 months. The hair growth and nourishing effects were evaluated according to the following criteria. During the test period, none of them complained of physical condition, scalp and hair abnormalities.

(1) Hair-growth effect About the change of the hair quality before and after sample intake, 4-step evaluation was performed according to the following reference | standard, and the average value was calculated | required.
3 points: A sufficient improvement in hair gloss, tension, smoothness and thickness was felt.
2 points: A clear improvement in hair gloss, tension, smoothness and thickness was felt.
1 point: A slight improvement in hair gloss, tension, smoothness and thickness was felt.
0 point: No improvement was felt in the gloss, tension, smoothness and thickness of the hair.

(2) Hair nourishing effect The number of hair loss during hair washing before and after sample intake was compared. As for the number of hair loss, the average value was obtained by collecting and counting hair loss at the time of washing once a day for 2 days before and after sample intake. Moreover, according to the change of the number of hair loss, 4-stage evaluation was performed according to the following reference | standard.
3 points: Reduction of hair loss is 50 or more.
2 points: Reduction in the number of hair loss is 30 or more and less than 50.
1 point: The decrease in the number of hair loss is 10 or more and less than 30.
0 point: decrease in the number of hair loss is less than 10 or increase in the number of hair loss.
The results are shown in Table 2.

From Table 2, a significant hair growth effect was confirmed in the combined intake of N-acetylglucosamine and a collagen peptide derived from fish (Example A) as compared with the individual intake (Comparative Examples K and L). In addition to N-acetylglucosamine and collagen peptides derived from fish , add chitin oligosaccharides, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract, or minerals derived from deep sea water. (Examples B to I) confirmed the tendency for the hair-restoring / hair-restoration effect to be further strengthened.

< Reference Example 1 >
Beverages were prepared according to a conventional method with the formulation shown in Table 3.

  This beverage had a moderate sweetness and a good taste. It is desirable to take this beverage on a daily basis (100 g).

< Reference Example 2 >
Cookies were baked according to a conventional method with the formulation shown in Table 4.

  This cookie was excellent in taste and texture and very delicious.

  Since the hair-restoring / hair-restoring composition for oral intake of the present invention can be easily blended into various foods and beverages, it can be applied to foods and beverages that can be expected to have a hair-restoring / hair-restoring effect.

Claims (4)

A composition for use in hair growth or hair restoration, comprising N-acetylglucosamine as an active ingredient and a collagen peptide derived from fish, which is for oral consumption. The hair growth / hair restoration composition for oral intake according to claim 1, wherein the collagen peptide derived from fish has an average molecular weight of 1,000 to 10,000. The hair-growth / hair-restoring composition for oral intake according to claim 1 or 2, wherein the collagen peptide derived from fish has a free amino acid content of 1 mass% or less and an arsenic content of 2 ppm or less. Furthermore, chitin oligosaccharides, shark cartilage extract, fucoidan, anserine, simnol sulfate, yeast extract, seaweed extract, and minerals selected from magnesium, sodium, calcium, potassium and zinc derived from deep sea water The hair growth / hair nourishing composition for oral consumption according to any one of claims 1 to 3, comprising one or more selected.