JP2013227228A - Muscle increasing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a muscle increasing agent effectively increasing muscles, having high safety, economical, and facilitating continuous use.SOLUTION: A muscle increasing agent contains a collagen peptide, as an active ingredient, obtained by hydrolysing collagen or gelatin derived from any one of a fish scale, fish bone, and fish skin, using a degrading enzyme derived from a bacillus.

Description

  The present invention relates to a muscle increasing agent.

In the lifestyles of modern people, there is an increased risk of causing obesity due to a decrease in exercise opportunities and westernization of dietary habits. Obesity can cause high blood pressure, hyperlipidemia, heart disease, diabetes, and the like, so it is extremely important to prevent and improve obesity from the viewpoint of maintaining health. Most obesity is caused by the fact that the energy consumed is higher than the energy consumed, so increasing the energy consumed leads to improvement in obesity. Here, since the energy required for basal metabolism increases when the muscle increases, the maintenance and promotion of health can be achieved by increasing the muscle.
Also, when muscles need to be increased or maintained, such as athletes, it is convenient if the muscles can be increased by eating.

Conventionally, many muscle augmentation agents containing proteins, peptides, amino acids and the like for increasing muscle, and processed foods, beverages, supplements, and steroids added with these are known (patents). Literatures 1-4).
However, conventional muscle-enhancing agents containing proteins, peptides, amino acids, etc. cannot increase muscles simply by ingestion, and can only increase muscles when used together with exercise. There were many. Exercise to eliminate obesity is painful and requires a strong will, and there are many situations in modern lifestyles where it is difficult to do continuous exercise that leads to muscle growth. Moreover, although the muscle increasing agent of patent document 1 does not make exercise | movement essential, since it contains the component derived from beef, there exist safety problems, such as an infectious disease. In addition, use of a steroid as a muscle enhancer is not desirable because it may have side effects such as liver dysfunction, cardiac dysfunction, masculinization, and hirsutism.
For this reason, development of a means capable of increasing muscles simply and safely regardless of the presence or absence of exercise is desired.

By the way, in recent years, various effects have been recognized on collagen-degrading peptides, and they are blended in pharmaceuticals and functional foods. For example, a collagen hydrolyzate derived from skin / bone such as bovine / pig / fish is applied to the treatment of osteoporosis or the like (Patent Documents 5 to 7), or a hydrolyzate of collagen such as pig or bovine is used as an anti-obesity agent. It is known to contain it as an active ingredient (Patent Documents 8 and 9) or to suppress an increase in blood glucose level using a peptide obtained by hydrolyzing fish scale-derived collagen (Patent Document 10).
In addition, the present inventors have previously reported that the body composition (body weight, muscle weight, fat mass) created by training is maintained in athletes who have continuously ingested fish scale-derived collagen. (Non-Patent Document 1).
However, it has not been known so far that muscles can be increased only by intake of collagen without using exercise.

JP 2004-075637 A JP 2004-292325 A JP 2004-256513 A International pamphlet 2004/075905 Japanese Patent Laid-Open No. 11-12192 JP 2005-343852 A JP 2008-231065 A JP 2009-102233 A Japanese Patent Laid-Open No. 2002-20212 JP 2002-326951 A

FOOD Style 21, July 2010 (Vol.14, No.7) reprint, p.1-4

As described above, it is desired to develop a means that can increase muscles simply and safely regardless of the presence or absence of exercise. In addition, it is preferable that the change in the body structure of increasing muscles is performed continuously over a long period of time because it does not place a burden on the body, so it is highly effective and of course an aspect that is safe, economical and easy to continue A method is required.
In view of such circumstances, an object of the present invention is to provide a muscle increasing agent that is effective for increasing muscles only by simple means of ingestion, is highly safe, economical, and easy to use continuously.

  The inventors of the present application have found that an effect of increasing muscles can be obtained by ingesting a collagen peptide obtained by hydrolyzing collagen or gelatin after intensive studies to solve the above problems.

That is, the present invention is as follows.
(1) A muscle increasing agent comprising a collagen peptide obtained by hydrolyzing collagen or gelatin as an active ingredient.
(2) The muscle increasing agent according to (1), wherein the collagen or gelatin is derived from any of fish scales, fish bones, and fish skins.
(3) The muscle increasing agent according to (2), wherein the fish scales, fish bones, and fish skin are derived from a perch fish.
(4) The muscle increasing agent according to any one of (1) to (3), wherein the hydrolysis is hydrolysis using a degrading enzyme derived from the genus Bacillus.
(5) The muscle increasing agent according to any one of (1) to (4), wherein the collagen peptide has a number average molecular weight of 500 to 1500.
(6) The muscle increasing agent according to any one of (1) to (5), which is taken together with exercise.
(7) The muscle increasing agent according to (6), wherein the exercise has a medium intensity or higher.

  By taking the muscle increasing agent of the present invention, the muscle can be effectively increased. Specifically, skeletal muscle increases. Moreover, since the muscle increasing agent of the present invention is preferably made from collagen or gelatin derived from one or more selected from fish scales, fish bones and fish skins, it is highly safe and economical. Moreover, since the muscle increasing agent of this invention is easy to acquire, the countermeasure against the increase in muscle which is easy to continue is implement | achieved. In addition, when used in combination with an exercise load that is easy to incorporate in daily life, the effect of increasing muscles can be further enhanced.

It is a graph showing the leg muscle wet weight value which corrected the body weight.

The muscle increasing agent of the present invention contains a collagen peptide as an active ingredient. Here, the collagen peptide means a peptide obtained by hydrolyzing collagen or gelatin.
Collagen or gelatin may be obtained from any of bones and skins such as cows, pigs, birds and sheep, fish scales, bones and skins. Particularly preferred is a collagen peptide derived from collagen or gelatin derived from one or more selected from fish scales, fish bones, and fish skins. Compared with collagen peptides derived from cattle and pigs, such peptides are safer than livestock infectious diseases, and in the case of fish scales and fish bones, they contain fat that makes the taste and smell worse. It is highly useful in that the ratio is very small.
The fish species for obtaining fish scales, fish bones, or fish skins (hereinafter referred to as fish scales) as raw materials for the muscle increasing agent of the present invention are not particularly limited, and include sardines, saury, tilapia, red sea bream, red sea bream, Pufferfish, mamakari, salmon, herring, carp, catfish, sea cucumber, etc. can be used, but from the viewpoint of a high content ratio of collagen in scales and a relatively large scale, a perch species is preferred.

The production conditions of the collagen peptide which is a hydrolyzate of collagen or gelatin in the present invention are not particularly limited. For example, a method of hydrolyzing and then hydrolyzing the enzyme after acid decalcification, or after extraction by pressurization Examples include enzymatic hydrolysis. In particular, since an enzyme hydrolyzate excellent in terms of quality can be obtained, in the present invention, a method of performing hydrothermal extraction after acid decalcification and enzymatic hydrolysis is preferable.
Specifically, the method of hydrolyzing after acid decalcification with hot water extraction is 40 to 20 to 20 times the weight ratio of wet or dry crude collagen deashed with hydrochloric acid or acetic acid and washed with water. In this method, collagen or gelatin is extracted for 1 to 20 hours using warm water or hot water at 100 ° C., and a proteolytic enzyme is added to the extract to enzymatically hydrolyze the extracted collagen or gelatin.

  As the proteolytic enzyme to be used, those that can be used in foods, are mass-produced with a high degradation rate, are available at a relatively low cost, and those that produce a small amount of amino acids are preferable. Degradation conditions that satisfy these conditions include neutral enzymes that can be used at a temperature of 40 ° C. or higher at which collagen is gelatinized and pH 5.0 to 8.0 that is easily decomposed, or pH 6.5 to 12. Alkaline enzymes are preferred, and specific examples include bacterial proteases derived from the genus Bacillus. Such bacterial proteases include, for example, protin P (registered trademark) (manufactured by Daiwa Kasei Co., Ltd.), orientase (registered trademark) 90N (manufactured by HIBI), and protease N Amano G (registered trademark). (Amano Enzyme Co., Ltd.) and the like. Examples of alkaline enzymes include Alcalase (registered trademark) (manufactured by Novo Nordisk Bioindustry Co., Ltd.), Biolase (registered trademark) SP-15FG (manufactured by Nagase Sangyo Co., Ltd.), Pro Leather (Registered trademark) FG-F (manufactured by Amano Pharmaceutical Co., Ltd.)

The quality of the collagen peptide in the present invention is further improved through a purification process. Activated carbon treatment is suitable for the purification step. The activated carbon treatment suitably performed in the present invention is not particularly limited, including the type of activated carbon and the amount used. As a preferable activated carbon treatment method in the present invention, 1 to 20% by weight of activated carbon with respect to the crude collagen is added to a solution containing the enzyme hydrolyzate, and the mixture is stirred for 15 to 360 minutes in a temperature range of 30 to 60 ° C. The method of repeating a process 1-5 times can be mentioned. In addition, a commercial item can be used for the activated carbon used for a refinement | purification process.
Activated carbon used for purification can be removed by filtering the solution after the activated carbon treatment. The filtration means is not particularly limited, and for example, commercially available diatomaceous earth can be removed by adding 1 to 50% by weight with respect to the crude collagen and filtering with a filter press or the like.

  The purified collagen peptide aqueous solution can be used as a liquid collagen peptide through a concentration step, or as a powdered collagen peptide through a concentration step and a dry powdering step. A suitable concentration process includes a vacuum concentration device such as a thin film evaporator, and a dry dryer process includes a spray dryer.

  The molecular weight of the collagen peptide which is an active ingredient of the muscle increasing agent of the present invention is not particularly limited, but is preferably 15000 or less, more preferably 300 to 300 in terms of number average molecular weight by gel chromatography (GPC) method. It is the range of 5000, More preferably, it is the range of 500-1500. If it is the said range, since the solubility with respect to water is favorable and it is anticipated that it will be absorbed rapidly in the body, it can expect the rapid expression of an effect.

  The muscle increasing agent of the present invention contains a collagen peptide obtained by hydrolyzing collagen or gelatin as an active ingredient, and the content of the active ingredient in the muscle increasing agent is particularly an amount that can exert a muscle increasing effect. There is no limit, and it may be adjusted as appropriate according to the frequency of intake, the amount of intake, and the purpose of use. When the effect is exhibited with a small amount of use, it is preferable that the content is large. That is, it is preferably contained in an amount of 1 to 100% by weight, more preferably 50 to 100% by weight, and further preferably 80 to 100% by weight of the whole muscle increasing agent.

The muscle increasing agent of the present invention can contain other components of the above active ingredients as long as the effects of the present invention are not impaired. Other components include, for example, physiologically acceptable excipients, binders, lubricants, disintegrants, suspending agents, emulsifiers, preservatives, stabilizers, preservatives, colorants, fragrances, and others. Various food additives and the like can be mentioned.
The muscle increasing agent of the present invention is taken orally. As the dosage form, forms such as tablets, coated tablets, powders, granules, capsules, microcapsules, syrups, and liquids can be applied, or forms added to food and drink regardless of liquid or solid But you can.

  When the muscle increasing agent of the present invention is added to food and drink, the form thereof is not particularly limited, but for example, beverages, energy drinks, confectionery, processed foods, fats and oils, dairy products, retort foods, range foods, frozen foods , Forms such as seasonings and health supplements. The shape and properties of the added food and drink are not particularly limited, and may be any of solid, semi-solid, gel, liquid, powder, etc., and any of tablets, capsules, liquids, granules, etc. .

In addition, when using the muscle increasing agent of the present invention, including addition to foods and drinks, other physiologically active ingredients of the agent can be blended or combined for the purpose of improving various functions. .
In particular, it is preferable to combine with other physiologically active ingredients and the like for the purpose of additive / synergistic improvement of the muscle increasing effect, assistance of the muscle increasing effect, improvement of absorbability, and the like. Other physiologically active ingredients for such purposes are not particularly limited as long as their physiological functions are clear, for example, other muscle-increasing agents, muscle-enhancing agents, functional foods for increasing muscles, etc. For example, whey protein, whey peptide, casein, soy protein (protein), soy peptide, wheat protein (protein), amino acids such as valine, leucine, isoleucine, arginine, citrulline, apple polyphenol, ursolic acid, tomato extract (fatty acid) , EMR (enzyme-treated rutin) catechin, creatine and the like.

Other physiologically active ingredients combined for the purpose of improving other functions include antioxidant ingredients, oily ingredients, various vitamins, minerals, amino acids and the like for nutritional enhancement.
The antioxidant component is not particularly limited.For example, tocopherols and derivatives thereof, tocotrienols and derivatives thereof, lignans such as sesamin, episesamin, sesaminol, sesamorin, sesamol and glycosides thereof, β -Carotenoids such as carotene and its derivatives, tannins such as gallic acid and ellagic acid and their derivatives, flavonoids such as flavone, catechin, quercetin and leucoanthocyanidin, quinones such as ubiquinone and vitamin K, ferla such as oryzanol Examples include acid derivatives and olive extracts. There are no particular restrictions on the types of vitamins, minerals, amino acids, and the like for nutritional enhancement, but those specified in the Food Additives Official Document are desirable.

  Although the specific example is listed below about the food / beverage to which the muscle increasing agent of this invention is added, this invention is not restrict | limited to these. There are no particular restrictions on the form of the cake, but examples include Japanese confectionery such as rice cake, rice cracker, rice cake, bun, rice cake, cookies, biscuits, crackers, pie, castella, donuts, pudding, sponge cake, waffles, butter cream, custard. Cream, cream puff, chocolate, chocolate confectionery, caramel, candy, chewing gum, jelly, hot cake, bread, confectionery such as bread, snacks such as potato chips, ice confectionery such as ice cream, popsicle, sherbet, water, lactic acid beverage, Lactic acid bacteria beverages, concentrated milk beverages, fruit juice beverages, fruit beverages, functional beverages, soft drinks such as carbonated beverages, taste products such as green tea, tea, coffee, cocoa and these beverages, milk, powdered milk, fermented milk, processed foods Dairy products such as milk and cheese, soy milk, tofu, etc. Processed foods, jams, fruit syrup pickles, pastes such as flower paste, peanut paste, fruit paste, pickles, udon noodles, pasta and other cereal products, ham, sausage, bacon, dry sausage, beef jerky, hamburg Livestock meat products such as fish ham, fish sausage, fish shell products such as kamaboko, chikuwa, hanpen, dried fish such as fish, shellfish, various sections such as salmon, salmon, salmon, salted sea urchin, squid, etc. Mirin, dried salmon, salmon and other salmon products, paste, small fish, shellfish, wild vegetables, shiitake mushrooms, kelp, etc., curry, stewed retort food, miso, soy sauce, sauce, ketchup, bouillon, grilled meat sauce, curry roux , Various ingredients such as stew, soup, dashi, etc., cooked rice, bread, oil and margarine, shortening, mayonnaise, dressing Of or oiled products, various ranges and frozen foods containing oils and fats can be cited.

The intake of the muscle increasing agent of the present invention varies depending on the age and weight of each person, but the daily intake for an adult is preferably 1 to 20 g, more preferably 5 to 15 g as the collagen peptide. Moreover, it may be taken in a plurality of times during a day, and can be easily taken into the daily life of the person who takes it.
By setting the intake amount within the above range, the action of increasing muscles is promoted.
In addition, the person (administration subject) who ingests the muscle increasing agent of the present invention is a person who wants to increase muscles. For example, an athlete, a person who wants to build physical strength, energy consumption for the purpose of eliminating obesity or improving health, etc. Some people want to increase.

The muscle increasing agent of the present invention is preferably taken in combination with exercise. The combined use of exercise improves the effect of increasing muscles.
Here, exercise indicates physical activity, and includes daily life activities such as exercise, labor, housework, commuting / commuting, hobbies, etc. that are carried out systematically and intentionally.
As the degree of load, a medium intensity exercise of 3 mets or more is preferable, and an exercise amount of 23 exercises (mets · hour) / week or more is preferable. “Mets” is a unit that expresses the intensity of exercise as shown in the “Exercise Guidelines 2006 for Health Promotion (Exercise Guide 2006)” by the Ministry of Health, Labor and Welfare The exercise intensity is represented by how many times it is based on the sitting and resting state (1 Mets). Similarly, “exercise (mets / hour)” is a unit representing the amount of exercise, and is obtained by multiplying exercise intensity (mets) by exercise time. For example, normal walking, bowling, volleyball, etc. correspond to 3 Mets exercise, fast walking, underwater exercise, bicycle rowing, etc. correspond to 4 Mets exercise, and 10.5 exercise if 3 Mets exercise is performed for 30 minutes / day every day. / Week.
When using the muscle increasing agent of the present invention in combination with exercise,
It may be before or after exercise and is not particularly limited.
The exercise frequency is preferably daily, but it is sufficient that the exercise frequency is 23 exercise (mets · hour) / week or more.

The muscle increasing agent of the present invention or food and drink containing the same may be labeled as being used for increasing muscle. The indication that it is used to increase muscles is a label that can be displayed mainly as a food for specified health use. For example, “Increase muscles to create a healthy body.” "Let's improve the body", "Muscle up to fat flammable body" and the like.
In addition, the muscle-increasing agent of the present invention or food and drink containing the same may be labeled as being taken together with exercise. The indication that it is ingested in combination with exercise is a notation that can be displayed mainly as a food for specified health use. For example, “Ingestion / drinking of this drug / food with exercise / "Increase the number of people", "Exercise after eating / drinking,""Walking exercise if you want to increase your muscles", and so on.
These indications can be attached to the container and packaging means by a known method, whereby the muscle-increasing agent of the present invention or foods and drinks containing it are used for increasing muscle, or Since it is clearly stated that it is to be taken in combination with the degree, the distinction from a normal agent or food and drink becomes clear.

In the present invention, the muscle increasing action means an action of increasing the amount of muscle, particularly skeletal muscle, and includes increasing the amount of the original muscle, maintaining the amount of muscle increased by training or the like without decreasing it. . Therefore, the muscle increasing effect of the present invention can be evaluated by measuring the muscle weight.
By ingesting the muscle increasing agent of the present invention, the muscle can be increased regardless of whether exercise is used or not, and this is effective for unreasonable muscle strengthening according to the lifestyle. Moreover, since the effect of increasing muscles can be further improved by using exercise together, it can be suitably used for body building of athletes and the like.

  Hereinafter, although an example is given and the present invention is explained still in detail, the present invention is not limited to these.

<1> Production of Muscle-Increasing Agent of the Present Invention Perch fish scales were washed and dried, and after decalcification with hydrochloric acid by a known method, washed with water (using ion-exchanged water) to obtain crude collagen. That is, 1,500 mL of 0.6 molar hydrochloric acid was added to 100 g of washed and dried scales (water content: 15% by weight) and stirred for 2 hours. Filtration through a 100-mesh net (decalcification solution pH 1.5), the solid content was put into 1 L of ion-exchanged water and stirred for 15 minutes, and this water washing / filtration treatment was repeated three times. The crude collagen content of the absolutely dry scale dried at 105 ° C. for 3 hours was 50% by weight, N: 17.4% by weight, residual calcium: 0.12% by weight, and residual phosphoric acid content: 0.37% by weight.
The crude collagen was suspended in 20 times (by weight) ion-exchanged water, and a protease N “Amano” G (produced by Amano Enzyme Co., Ltd.), a proteolytic enzyme derived from Bacillus subtilis.
(Registered Trademark) was added at a ratio of 1.0% by weight to the crude collagen and hydrolyzed at 50 ° C. for 3 hours. Thereafter, activated carbon “Tamura S manufactured by Futamura Chemical Co., Ltd.” (registered trademark) was added in an amount of 0.4% by weight to the crude collagen, and deodorized and decolored by stirring at 40 ° C. for 30 minutes. The solution was cooled to room temperature, filtered through diatomaceous earth, concentrated and powdered to obtain a collagen peptide.

When the number average molecular weight of the collagen peptide obtained above was measured by gel filtration using high performance liquid chromatography according to the Pagii Method 20-2 “Average Molecular Weight” defined by the Photographic Gelatin Test Method Joint Council, 1010.
1) Take 0.2 g of sample into a 100 mL volumetric flask and add eluent (equal mixture of 0.1 mol / L potassium dihydrogen phosphate solution and 0.1 mol / L disodium hydrogen phosphate solution) for 1 hour. After swelling, it was dissolved by heating at 40 ° C. for 60 minutes. After cooling to room temperature, the eluent was added to the marked line.
2) The obtained solution was diluted exactly 10 times with an eluent to prepare a test solution.
3) A chromatogram was obtained by gel filtration using the test solution.
Column: Two Shodex Asahipak GS 620 7G in series Eluent flow rate: 1.0 mL / min
Column temperature: 50 ° C
Detection method: Absorbance at a measurement wavelength of 230 nm 4) The storage time was taken on the horizontal axis, the corresponding absorbance value at 230 nm was taken on the vertical axis, a molecular distribution curve of the sample was created, and the number average molecular weight was calculated.

<2> Muscle-increasing action test <2-1> Test method A muscle-increasing action test was performed using the muscle-increasing agent of the present invention produced as in the above production example.
As test animals, 5-week-old male Wistar rats were used. Casein was used as the protein source of the feed, and the protein content was 20% by weight (standard diet; Moderate: M) based on the weight of the feed. Moreover, the group (MC) which replaced 30 weight part in 100 weight part of casein in feed with the muscle increasing agent of this invention was provided. Furthermore, each group was divided into a non-exercise group and an exercise group (Ex), and a total of 8 groups shown in Table 1 were obtained.

In each group, after the preliminary breeding for 1 week, the test period was 11 weeks.
During the test period, rats were raised in an environment of 23 ± 1 ° C., humidity 50 ± 5%, and light / dark cycle every 12 hours (light period 8:00 am to 8:00 pm). Feed and deionized distilled water having the composition shown in 2 were freely ingested.

In addition, as an exercise load during the test period, a running exercise using a treadmill for small animals (KN-73, Natsume Seisakusho, Tokyo) was performed at a frequency of 6 times a week. The exercise adaptation period was set for 19 days, and the main run was carried out for a total of 55 days from the 20th day after the start of the experiment (25 m / min was 60 min). The running speed was gradually increased (25 to 27 m / min), and finally, 25 m / min was continuously run for 15 minutes and 27 m / min for 45 minutes for a total of 60 minutes. A preparatory exercise (15 m / min for 5 minutes) and a cool-down (15 m / min for 5 minutes) were performed before and after the exercise, and the total running time per day was 70 minutes. In the test animals of this example, a running speed of 25 to 27 m / min corresponds to 60 to 70% VO ₂ max (Wheeler DL, Graves JE, Miller GL, Vander-Griend RE, Wronski TJ, Powers SK, Park HM. 1995. Effects
Med Sci Sports Exerc 27: 520-529), which corresponds to a medium-strength exercise load in humans.

Body weight and feed intake were measured every 2 days. On the last day of breeding, rats fasted for 12 hours were dissected under diethyl ether anesthesia, and right skeletal muscles (quadriceps, gastrocnemius, soleus, plantar muscles) were collected and wet weight was measured.
The measured values were statistically processed and all expressed as mean ± SE. The statistical software was SPSS (version 12.0J; Inc., Chicago, IL, USA), and the difference between each group was examined using a one-way analysis of variance. Multiple comparisons between groups were performed using the Scheffe method with a risk rate of 5%.

<2-2> Each evaluation item and result Table 3 shows final body weight, feed intake, and feed efficiency.
There was no difference between groups in body weight at the start of the experiment. In the final body weight and feed intake, the exercise group (MEx and MCEx) showed significantly lower values than the non-exercise group (M and MC), respectively, regardless of the presence or absence of the muscle increasing agent of the present invention. In addition, in the feed efficiency, the exercise group (MEx and MCEx) showed a significantly lower value than the non-exercise group (M and MC), respectively, and the group in which intake of the muscle increasing agent of the present invention and exercise load were combined ( MCEx) was the lowest.

FIG. 1 shows wet weights of the right skeletal muscles (quadriceps, gastrocnemius, soleus, plantar muscles) as weight corrected values.
Regarding the quadriceps muscle, the MC group showed higher values than the M group although there was no significant difference. In addition, the group (MCEx) in which the muscle increasing agent of the present invention and exercise were used in combination showed a significantly higher value than the M group. Regarding the gastrocnemius, soleus and plantar muscles, the MC group showed higher values than the M group although there was no significant difference. In addition, the group (MCEx) in which the muscle increasing agent of the invention and exercise were used in combination showed a high value or a high value tendency although there was no significant difference.
From these results, it was shown that by taking the muscle increasing agent of the present invention, muscles can be increased without special diet or exercise. Furthermore, it was shown that muscles can be increased further by exercising in parallel with ingestion of this drug.

  According to the present invention, a muscle increasing agent that is effective in increasing muscle, is safe, economical, and easy to use continuously is provided.

Claims (7)

  A muscle increasing agent comprising a collagen peptide obtained by hydrolyzing collagen or gelatin as an active ingredient.   The muscle increasing agent according to claim 1, wherein the collagen or gelatin is derived from any of fish scales, fish bones, and fish skins.   The muscle increasing agent according to claim 2, wherein the fish scales, fish bones, and fish skin are derived from a perch fish.   The muscle increasing agent according to any one of claims 1 to 3, wherein the hydrolysis is hydrolysis using a degrading enzyme derived from the genus Bacillus.   The muscle increasing agent as described in any one of Claims 1-4 whose number average molecular weights of the said collagen peptide are 500-1500.   The muscle increasing agent according to any one of claims 1 to 5, which is taken together with exercise.   The muscle increasing agent of Claim 6 whose said exercise | movement is more than medium intensity | strength.

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