JP3947653B2 - プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用 - Google Patents

プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用 Download PDF

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JP3947653B2
JP3947653B2 JP2000507811A JP2000507811A JP3947653B2 JP 3947653 B2 JP3947653 B2 JP 3947653B2 JP 2000507811 A JP2000507811 A JP 2000507811A JP 2000507811 A JP2000507811 A JP 2000507811A JP 3947653 B2 JP3947653 B2 JP 3947653B2
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protease
trypsin
zymogen
proteases
precursor
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Japanese (ja)
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JP2001514003A5 (enExample
JP2001514003A (ja
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コペッツキ、エルハルト
ホプフナー、カール‐ペーター
ボード、ウォルフラム
フーバー、ロバート
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ロシュ ダイアグノスティックス ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000507811A 1997-08-22 1998-08-12 プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用 Expired - Fee Related JP3947653B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP97114513 1997-08-22
EP97117816 1997-10-15
EP97117816.5 1997-10-15
EP97114513.1 1997-10-15
PCT/EP1998/005094 WO1999010503A1 (de) 1997-08-22 1998-08-12 Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung

Publications (3)

Publication Number Publication Date
JP2001514003A JP2001514003A (ja) 2001-09-11
JP2001514003A5 JP2001514003A5 (enExample) 2005-12-22
JP3947653B2 true JP3947653B2 (ja) 2007-07-25

Family

ID=26145739

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000507811A Expired - Fee Related JP3947653B2 (ja) 1997-08-22 1998-08-12 プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用

Country Status (9)

Country Link
EP (1) EP1012307B1 (enExample)
JP (1) JP3947653B2 (enExample)
AT (1) ATE294869T1 (enExample)
AU (1) AU9260598A (enExample)
CA (1) CA2301698A1 (enExample)
DE (1) DE59812768D1 (enExample)
DK (1) DK1012307T3 (enExample)
IL (1) IL134653A0 (enExample)
WO (1) WO1999010503A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6159722A (en) * 1997-12-03 2000-12-12 Boehringer Mannheim Gmbh Chimeric serine proteases
GB9911689D0 (en) * 1999-05-19 1999-07-21 Medical Res Council Refolding method
TR200200048T2 (tr) 1999-08-09 2002-04-22 Biochemie Gesellschaft M.B.H. Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.
SK287488B6 (sk) * 1999-08-09 2010-11-08 Sandoz Ag Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou
AU7053500A (en) * 1999-09-15 2001-04-17 Eli Lilly And Company Chymotrypsin-free trypsin
WO2001066771A2 (en) * 2000-03-03 2001-09-13 Zymogenetics, Inc. Human serine protease
JP4159878B2 (ja) 2001-02-01 2008-10-01 エフ．ホフマン−ラ ロシュ アーゲー 組換えトリプシンの製造方法
DK1546302T3 (da) 2002-08-30 2009-12-14 Novozymes Inc Fremgangsmåder til fremstilling af pattedyrs-trypsiner
JP2006507813A (ja) * 2002-10-04 2006-03-09 シエーリング アクチエンゲゼルシャフト 置換体活性化配列を有する修飾されたヘプシン分子及びその使用
MXPA05013321A (es) 2003-06-11 2006-03-09 Schering Ag Nuevas moleculas modificadas de corina que tienen secuencias de activacion sustitutiva y sus usos.
JP2015092852A (ja) * 2013-11-12 2015-05-18 倉敷紡績株式会社 生体組織分散用組成物
EP3604508A4 (en) * 2017-03-24 2021-01-20 Kaneka Corporation METHOD OF PRODUCTION OF PROTEASE IN YEAST
EP4623086A1 (en) 2022-11-23 2025-10-01 F. Hoffmann-La Roche AG Method for increasing recombinant protein expression

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
DE4140699A1 (de) * 1991-01-11 1992-07-16 Boehringer Mannheim Gmbh Rekombinante iga-protease
CA2102673A1 (en) * 1992-11-13 1994-05-14 Michael G. Greaney Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
JP3920331B2 (ja) * 1994-12-09 2007-05-30 ノボ ノルディスク アクティーゼルスカブ 細菌において細胞外タンパク質を製造するための方法
JPH11507207A (ja) * 1995-06-16 1999-06-29 ノボ ノルディスク アクティーゼルスカブ トリプシン（トリプシノーゲン）の製造法

Also Published As

Publication number Publication date
DE59812768D1 (de) 2005-06-09
AU9260598A (en) 1999-03-16
ATE294869T1 (de) 2005-05-15
WO1999010503A1 (de) 1999-03-04
IL134653A0 (en) 2001-04-30
EP1012307A1 (de) 2000-06-28
EP1012307B1 (de) 2005-05-04
CA2301698A1 (en) 1999-03-04
DK1012307T3 (da) 2005-09-05
JP2001514003A (ja) 2001-09-11

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