DE59812768D1 - Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung - Google Patents
Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendungInfo
- Publication number
- DE59812768D1 DE59812768D1 DE59812768T DE59812768T DE59812768D1 DE 59812768 D1 DE59812768 D1 DE 59812768D1 DE 59812768 T DE59812768 T DE 59812768T DE 59812768 T DE59812768 T DE 59812768T DE 59812768 D1 DE59812768 D1 DE 59812768D1
- Authority
- DE
- Germany
- Prior art keywords
- protease
- zymogenic
- precursor
- active
- proteases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 7
- 239000004365 Protease Substances 0.000 title abstract 7
- 102000035195 Peptidases Human genes 0.000 title abstract 2
- 230000001376 precipitating effect Effects 0.000 title 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 5
- 230000003462 zymogenic effect Effects 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 3
- 239000002243 precursor Substances 0.000 abstract 3
- 230000007017 scission Effects 0.000 abstract 3
- 101710140962 Capsid scaffolding protein Proteins 0.000 abstract 2
- 210000003000 inclusion body Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6432—Coagulation factor Xa (3.4.21.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21006—Coagulation factor Xa (3.4.21.6)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97114513 | 1997-08-22 | ||
| EP97117816 | 1997-10-15 | ||
| PCT/EP1998/005094 WO1999010503A1 (de) | 1997-08-22 | 1998-08-12 | Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE59812768D1 true DE59812768D1 (de) | 2005-06-09 |
Family
ID=26145739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE59812768T Expired - Lifetime DE59812768D1 (de) | 1997-08-22 | 1998-08-12 | Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1012307B1 (enExample) |
| JP (1) | JP3947653B2 (enExample) |
| AT (1) | ATE294869T1 (enExample) |
| AU (1) | AU9260598A (enExample) |
| CA (1) | CA2301698A1 (enExample) |
| DE (1) | DE59812768D1 (enExample) |
| DK (1) | DK1012307T3 (enExample) |
| IL (1) | IL134653A0 (enExample) |
| WO (1) | WO1999010503A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6159722A (en) | 1997-12-03 | 2000-12-12 | Boehringer Mannheim Gmbh | Chimeric serine proteases |
| GB9911689D0 (en) * | 1999-05-19 | 1999-07-21 | Medical Res Council | Refolding method |
| IL147411A0 (en) | 1999-08-09 | 2002-08-14 | Biochemie Gmbh | Production of proteins by autoproteolytic cleavage |
| SK287488B6 (sk) * | 1999-08-09 | 2010-11-08 | Sandoz Ag | Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou |
| WO2001019970A2 (en) * | 1999-09-15 | 2001-03-22 | Eli Lilly And Company | Chymotrypsin-free trypsin |
| WO2001066771A2 (en) * | 2000-03-03 | 2001-09-13 | Zymogenetics, Inc. | Human serine protease |
| DK1399568T3 (da) | 2001-02-01 | 2006-03-27 | Hoffmann La Roche | Fremgangsmåde til fremstilling af rekombinant trypsin |
| DK1546302T3 (da) | 2002-08-30 | 2009-12-14 | Novozymes Inc | Fremgangsmåder til fremstilling af pattedyrs-trypsiner |
| JP2006507813A (ja) * | 2002-10-04 | 2006-03-09 | シエーリング アクチエンゲゼルシャフト | 置換体活性化配列を有する修飾されたヘプシン分子及びその使用 |
| CN101109011A (zh) * | 2003-06-11 | 2008-01-23 | 舍林股份公司 | 新的具有取代活化序列的经修饰的corin分子及其用途 |
| JP2015092852A (ja) * | 2013-11-12 | 2015-05-18 | 倉敷紡績株式会社 | 生体組織分散用組成物 |
| WO2018174231A1 (ja) * | 2017-03-24 | 2018-09-27 | 株式会社カネカ | 酵母におけるプロテアーゼの製造方法 |
| JP2025538565A (ja) | 2022-11-23 | 2025-11-28 | エフ． ホフマン－ラ ロシュ アーゲー | 組換えタンパク質発現を増加させるための方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340740C (en) * | 1987-12-08 | 1999-09-14 | Eileen R. Mulvihill | Co-expression in eukaryotic cells |
| DE4140699A1 (de) * | 1991-01-11 | 1992-07-16 | Boehringer Mannheim Gmbh | Rekombinante iga-protease |
| CA2102673A1 (en) * | 1992-11-13 | 1994-05-14 | Michael G. Greaney | Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith |
| JP3920331B2 (ja) * | 1994-12-09 | 2007-05-30 | ノボ ノルディスク アクティーゼルスカブ | 細菌において細胞外タンパク質を製造するための方法 |
| EP0833898A1 (en) * | 1995-06-16 | 1998-04-08 | Novo Nordisk A/S | A process for producing trypsin (trypsinogen) |
-
1998
- 1998-08-12 JP JP2000507811A patent/JP3947653B2/ja not_active Expired - Fee Related
- 1998-08-12 CA CA002301698A patent/CA2301698A1/en not_active Abandoned
- 1998-08-12 DK DK98945214T patent/DK1012307T3/da active
- 1998-08-12 WO PCT/EP1998/005094 patent/WO1999010503A1/de not_active Ceased
- 1998-08-12 AT AT98945214T patent/ATE294869T1/de active
- 1998-08-12 DE DE59812768T patent/DE59812768D1/de not_active Expired - Lifetime
- 1998-08-12 EP EP98945214A patent/EP1012307B1/de not_active Expired - Lifetime
- 1998-08-12 AU AU92605/98A patent/AU9260598A/en not_active Abandoned
- 1998-08-12 IL IL13465398A patent/IL134653A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1012307A1 (de) | 2000-06-28 |
| IL134653A0 (en) | 2001-04-30 |
| WO1999010503A1 (de) | 1999-03-04 |
| AU9260598A (en) | 1999-03-16 |
| ATE294869T1 (de) | 2005-05-15 |
| EP1012307B1 (de) | 2005-05-04 |
| JP3947653B2 (ja) | 2007-07-25 |
| DK1012307T3 (da) | 2005-09-05 |
| JP2001514003A (ja) | 2001-09-11 |
| CA2301698A1 (en) | 1999-03-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |