JP3644641B2 - 核酸回収方法 - Google Patents

核酸回収方法 Download PDF

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Publication number
JP3644641B2
JP3644641B2 JP2003060983A JP2003060983A JP3644641B2 JP 3644641 B2 JP3644641 B2 JP 3644641B2 JP 2003060983 A JP2003060983 A JP 2003060983A JP 2003060983 A JP2003060983 A JP 2003060983A JP 3644641 B2 JP3644641 B2 JP 3644641B2
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Japan
Prior art keywords
nucleic acid
eluent
solid phase
rna
temperature
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Expired - Fee Related
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JP2003060983A
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English (en)
Japanese (ja)
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JP2004267077A5 (https=
JP2004267077A (ja
Inventor
智也 桜井
敏昭 横林
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Hitachi High Tech Corp
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Hitachi High Technologies Corp
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Priority to JP2003060983A priority Critical patent/JP3644641B2/ja
Priority to US10/732,223 priority patent/US20040175735A1/en
Priority to EP03028759A priority patent/EP1454983A1/en
Priority to CNA2004100042509A priority patent/CN1526720A/zh
Publication of JP2004267077A publication Critical patent/JP2004267077A/ja
Application granted granted Critical
Publication of JP3644641B2 publication Critical patent/JP3644641B2/ja
Publication of JP2004267077A5 publication Critical patent/JP2004267077A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2003060983A 2003-03-07 2003-03-07 核酸回収方法 Expired - Fee Related JP3644641B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003060983A JP3644641B2 (ja) 2003-03-07 2003-03-07 核酸回収方法
US10/732,223 US20040175735A1 (en) 2003-03-07 2003-12-11 Process for recovery of nucleic acids
EP03028759A EP1454983A1 (en) 2003-03-07 2003-12-12 Process for recovery of nucleic acids
CNA2004100042509A CN1526720A (zh) 2003-03-07 2004-02-12 核酸回收方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003060983A JP3644641B2 (ja) 2003-03-07 2003-03-07 核酸回収方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2004343829A Division JP4073430B2 (ja) 2004-11-29 2004-11-29 核酸回収方法

Publications (3)

Publication Number Publication Date
JP2004267077A JP2004267077A (ja) 2004-09-30
JP3644641B2 true JP3644641B2 (ja) 2005-05-11
JP2004267077A5 JP2004267077A5 (https=) 2005-07-21

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Family Applications (1)

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JP2003060983A Expired - Fee Related JP3644641B2 (ja) 2003-03-07 2003-03-07 核酸回収方法

Country Status (4)

Country Link
US (1) US20040175735A1 (https=)
EP (1) EP1454983A1 (https=)
JP (1) JP3644641B2 (https=)
CN (1) CN1526720A (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009162733A (ja) * 2008-01-10 2009-07-23 Hitachi High-Technologies Corp Ｂ／ｆ分離の洗浄方法及びｂ／ｆ分離の洗浄装置

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4241183B2 (ja) * 2003-05-19 2009-03-18 株式会社日立ハイテクノロジーズ 核酸回収方法及び核酸回収キット
JP2010038596A (ja) * 2008-08-01 2010-02-18 Enplas Corp 流体取扱装置の操作方法
JP2010158190A (ja) * 2009-01-07 2010-07-22 Hitachi High-Technologies Corp 核酸回収方法及び核酸回収装置
CN109797093B (zh) * 2019-03-11 2023-09-29 中国科学院苏州生物医学工程技术研究所 一种用于核酸提取的耗材、装置及核酸提取方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
US6180778B1 (en) * 1994-02-11 2001-01-30 Qiagen Gmbh Process for the separation of double-stranded/single-stranded nucleic acid structures
DE19743518A1 (de) * 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
JPH11266864A (ja) * 1998-03-19 1999-10-05 Hitachi Ltd 核酸の精製方法および精製用装置
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6492162B1 (en) * 1998-10-27 2002-12-10 Hitachi, Ltd. Apparatus for the recovery of nucleic acids
CA2270106C (en) * 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
PT1232502E (pt) * 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
JP3602071B2 (ja) * 2001-06-05 2004-12-15 株式会社日立製作所 核酸の精製分離方法
AU2002359522A1 (en) * 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009162733A (ja) * 2008-01-10 2009-07-23 Hitachi High-Technologies Corp Ｂ／ｆ分離の洗浄方法及びｂ／ｆ分離の洗浄装置

Also Published As

Publication number Publication date
EP1454983A1 (en) 2004-09-08
US20040175735A1 (en) 2004-09-09
JP2004267077A (ja) 2004-09-30
CN1526720A (zh) 2004-09-08

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