JP3256539B2 - 連結分子を介する３’―尾分子付オリゴヌクレオチドの固体支持体合成 - Google Patents

連結分子を介する３’―尾分子付オリゴヌクレオチドの固体支持体合成

Info

Publication number
JP3256539B2
JP3256539B2 JP51534191A JP51534191A JP3256539B2 JP 3256539 B2 JP3256539 B2 JP 3256539B2 JP 51534191 A JP51534191 A JP 51534191A JP 51534191 A JP51534191 A JP 51534191A JP 3256539 B2 JP3256539 B2 JP 3256539B2
Authority
JP
Japan
Prior art keywords
group
molecule
oligonucleotide
tail
linking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP51534191A
Other languages
English (en)
Japanese (ja)
Other versions
JPH06500556A (ja
Inventor
リード、マイケル・ダブリュー
メイヤー、リッチ・ビイ・ジュニア
ピートリー、チャールズ・アール
ターボン、ジョン・シー
Original Assignee
エポック・ファーマシューティカルズ・インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エポック・ファーマシューティカルズ・インコーポレイテッド filed Critical エポック・ファーマシューティカルズ・インコーポレイテッド
Publication of JPH06500556A publication Critical patent/JPH06500556A/ja
Application granted granted Critical
Publication of JP3256539B2 publication Critical patent/JP3256539B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D207/10—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D207/12—Oxygen or sulfur atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
JP51534191A 1990-08-28 1991-08-28 連結分子を介する３’―尾分子付オリゴヌクレオチドの固体支持体合成 Expired - Fee Related JP3256539B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US57434890A 1990-08-28 1990-08-28
US71414291A 1991-06-10 1991-06-10
US574,348 1991-06-10
US714,142 1991-06-10
CA002089588A CA2089588A1 (en) 1990-08-28 1993-02-16 Solid support synthesis of 3'-tailed oligonucleotides via a linking molecule

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2000246403A Division JP3284127B2 (ja) 1990-08-28 2000-08-15 オリゴヌクレオチドの固相支持体

Publications (2)

Publication Number Publication Date
JPH06500556A JPH06500556A (ja) 1994-01-20
JP3256539B2 true JP3256539B2 (ja) 2002-02-12

Family

ID=27169350

Family Applications (1)

Application Number Title Priority Date Filing Date
JP51534191A Expired - Fee Related JP3256539B2 (ja) 1990-08-28 1991-08-28 連結分子を介する３’―尾分子付オリゴヌクレオチドの固体支持体合成

Country Status (4)

Country Link
EP (1) EP0547142A1 (de)
JP (1) JP3256539B2 (de)
CA (1) CA2089588A1 (de)
WO (1) WO1992003464A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4770093A (en) * 1992-07-02 1994-01-31 Hybridon, Inc. Self-stabilized oligonucleotides as therapeutic agents
DE4239531A1 (de) * 1992-11-25 1994-05-26 Gabor Dr Igloi Reagenz zur Kupplung verschiedener Substanzen an Nukleinsäuren sowie Verfahren zu dessen Herstellung
BE1007918A3 (fr) * 1993-02-19 1995-11-21 Wallone Region Sondes d'acides nucleiques chimiquement modifiees en 5'(oh) et/ou en 3'(oh) en vue de l'introduction en ces positions d'un ou plusieurs elements de marquage non radioactif et procede de preparation.
US6576752B1 (en) 1997-02-14 2003-06-10 Isis Pharmaceuticals, Inc. Aminooxy functionalized oligomers
US6639062B2 (en) 1997-02-14 2003-10-28 Isis Pharmaceuticals, Inc. Aminooxy-modified nucleosidic compounds and oligomeric compounds prepared therefrom
FR2765877B1 (fr) * 1997-07-10 1999-09-10 Oreal Composition comprenant une dispersion aqueuse de vesicules lipidiques a base de carbamates a chaine cholesteryle, utilisation notamment en cosmetique et nouveaux composes
EP2248820B1 (de) * 2000-11-29 2014-01-08 Glen Research Corporation Universalträger für Oligonukleotidsynthese
JP2005504020A (ja) * 2001-07-03 2005-02-10 アイシス・ファーマシューティカルス・インコーポレーテッド ヌクレアーゼ耐性キメラオリゴヌクレオチド
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
WO2003064625A2 (en) 2002-02-01 2003-08-07 Sequitur, Inc. Oligonucleotide compositions with enhanced efficiency
US8017762B2 (en) 2003-04-17 2011-09-13 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
ES2702942T3 (es) 2003-04-17 2019-03-06 Alnylam Pharmaceuticals Inc Agentes de ARNi modificados
CA2522349A1 (en) * 2003-04-17 2004-11-04 Alnylam Pharmaceuticals, Inc. Protected monomers
US7723509B2 (en) 2003-04-17 2010-05-25 Alnylam Pharmaceuticals IRNA agents with biocleavable tethers
US7851615B2 (en) * 2003-04-17 2010-12-14 Alnylam Pharmaceuticals, Inc. Lipophilic conjugated iRNA agents
US8796436B2 (en) 2003-04-17 2014-08-05 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
JP4584986B2 (ja) 2004-04-27 2010-11-24 アルニラム ファーマスーティカルズ インコーポレイテッド ２−アリールプロピル部分を含む１本鎖及び２本鎖オリゴヌクレオチド
EP1791567B1 (de) 2004-08-10 2015-07-29 Alnylam Pharmaceuticals Inc. Chemisch modifizierte oligonucleotide
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US7960357B2 (en) 2005-10-07 2011-06-14 California Institute Of Technology PKR activation via hybridization chain reaction
WO2007094218A1 (ja) * 2006-02-16 2007-08-23 Gifu University 修飾オリゴヌクレオチド
CA2663601C (en) * 2006-09-22 2014-11-25 Dharmacon, Inc. Duplex oligonucleotide complexes and methods for gene silencing by rna interference
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
CN104975020B (zh) 2008-02-11 2020-01-17 菲奥医药公司 经修饰的RNAi多核苷酸及其用途
US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
WO2010008582A2 (en) 2008-07-18 2010-01-21 Rxi Pharmaceuticals Corporation Phagocytic cell drug delivery system
EP2949752B1 (de) 2008-09-22 2017-12-20 RXi Pharmaceuticals Corporation Selbstfreisetzende rnai-verbindungen von reduzierter grösse
US9074211B2 (en) 2008-11-19 2015-07-07 Rxi Pharmaceuticals Corporation Inhibition of MAP4K4 through RNAI
WO2010078536A1 (en) 2009-01-05 2010-07-08 Rxi Pharmaceuticals Corporation Inhibition of pcsk9 through rnai
WO2010090762A1 (en) 2009-02-04 2010-08-12 Rxi Pharmaceuticals Corporation Rna duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
WO2011119887A1 (en) 2010-03-24 2011-09-29 Rxi Pharmaceuticals Corporation Rna interference in dermal and fibrotic indications
EP3578183B1 (de) 2010-03-24 2021-09-08 Phio Pharmaceuticals Corp. Rna-interferenz bei augenerkrankungen
RU2615143C2 (ru) 2010-03-24 2017-04-04 Адвирна Самодоставляющие PHKi соединения уменьшенного размера
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
EP3079707A4 (de) 2013-12-02 2017-10-18 RXi Pharmaceuticals Corporation Immuntherapie für krebs
CN105960265A (zh) 2013-12-04 2016-09-21 阿克赛医药公司 利用经化学修饰的寡核苷酸处理伤口愈合的方法
WO2015125845A1 (ja) * 2014-02-20 2015-08-27 塩野義製薬株式会社 含窒素非芳香族複素環を含む核酸のリン酸部位修飾
CA2947270A1 (en) 2014-04-28 2015-11-05 Rxi Pharmaceuticals Corporation Methods for treating cancer using nucleic acids targeting mdm2 or mycn
JP2017514908A (ja) 2014-05-01 2017-06-08 アールエックスアイ ファーマシューティカルズ コーポレーション 核酸分子を利用する目の前部における障害の処置のための方法
US10900039B2 (en) 2014-09-05 2021-01-26 Phio Pharmaceuticals Corp. Methods for treating aging and skin disorders using nucleic acids targeting Tyr or MMP1
JP6983752B2 (ja) 2015-07-06 2021-12-17 フィオ ファーマシューティカルズ コーポレーションPhio Pharmaceuticals Corp. スーパーオキシドディスムターゼ１（ｓｏｄ１）を標的とする核酸分子
US10808247B2 (en) 2015-07-06 2020-10-20 Phio Pharmaceuticals Corp. Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach
WO2017070151A1 (en) 2015-10-19 2017-04-27 Rxi Pharmaceuticals Corporation Reduced size self-delivering nucleic acid compounds targeting long non-coding rna
EP3943613A1 (de) 2016-07-05 2022-01-26 California Institute of Technology Hybridisierungskettenreaktion mit fraktioniertem initiator
EP3507296B1 (de) 2016-08-30 2022-10-12 California Institute of Technology Immunohistochemie mittels hybridisierungskettenreaktion
EP4055167A2 (de) 2019-11-08 2022-09-14 Phio Pharmaceuticals Corp. Auf das bromodomainhaltige protein 4 (brd4) abzielende chemisch modifizierte oligonukleotide für die immuntherapie
EP4085136A1 (de) 2019-12-31 2022-11-09 Phio Pharmaceuticals Corp. Chemisch modifizierte oligonukleotide mit verbesserter systemischer abgabe
WO2021177419A1 (ja) * 2020-03-04 2021-09-10 国立大学法人京都大学 アンチセンスオリゴヌクレオチド医薬のスクリーニング方法
WO2022164796A1 (en) 2021-01-26 2022-08-04 California Institute Of Technology Allosteric conditional guide rnas for cell-selective regulation of crispr/cas
CN118043459A (zh) 2021-08-04 2024-05-14 菲奥医药公司 经化学修饰的寡核苷酸
WO2023015264A1 (en) 2021-08-04 2023-02-09 Phio Pharmaceuticals Corp. Immunotherapy of cancer utilizing natural killer cells treated with chemically modified oligonucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3856406T2 (de) * 1987-10-28 2000-10-19 Florey Howard Inst Oligonucleotid-polyamid konjugate

Also Published As

Publication number Publication date
JPH06500556A (ja) 1994-01-20
WO1992003464A1 (en) 1992-03-05
EP0547142A4 (de) 1995-01-18
EP0547142A1 (de) 1993-06-23
CA2089588A1 (en) 1994-08-17

Similar Documents

Publication Publication Date Title
JP3256539B2 (ja) 連結分子を介する３’―尾分子付オリゴヌクレオチドの固体支持体合成
US5419966A (en) Solid support for synthesis of 3'-tailed oligonucleotides
US5512667A (en) Trifunctional intermediates for preparing 3'-tailed oligonucleotides
JP4127870B2 (ja) ３’−標識ポリヌクレオチドの直接合成用の固体支持体試薬
US5646126A (en) Sterol modified oligonucleotide duplexes having anticancer activity
KR101032008B1 (ko) 폴리뉴클레오티드 표지 시약
Pourceau et al. Azide solid support for 3′-conjugation of oligonucleotides and their circularization by click chemistry
JP2597698B2 (ja) オリゴヌクレオチド‐ポリアミド コンジュゲート
US5410068A (en) Succinimidyl trityl compounds and a process for preparing same
JP3684182B2 (ja) 核酸を標識するための新しい試薬
EP0466773A4 (en) Coumarin derivatives for use as nucleotide crosslinking reagents
JPH01500748A (ja) アミノ−誘導化亜リン酸エステル及びリン酸エステル結合剤、ホスホルアミダイト前駆体及び有用な複合体
JP2002540118A (ja) キシロ−ｌｎａ類似体
JP2007131642A (ja) 固体支持体上での標識化オリゴヌクレオチドおよびアナログの合成のための方法および組成物
CN101454315A (zh) 用于合成罗丹明-标记的寡核苷酸的试剂
JP2001509784A (ja) 非ヌクレオチド連結試薬
US6166239A (en) Oligonucleotide protecting groups
Guzaev et al. Solid support synthesis of ester linked hydrophobic conjugates of oligonucleotides
US6303799B1 (en) Polynucleotide crosslinking agents
JP3284127B2 (ja) オリゴヌクレオチドの固相支持体
US7329515B2 (en) Solid support for the synthesis of 3′-amino oligonucleotides
US7943758B2 (en) 3′-end nucleoside unit comprising phosphoramidite
JP2005255528A (ja) 新規炭酸エステル誘導体及びそれらを利用する核酸合成法

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S633 Written request for registration of reclamation of name

Free format text: JAPANESE INTERMEDIATE CODE: R313633

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20081130

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20081130

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20091130

Year of fee payment: 8

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101130

Year of fee payment: 9

LAPS Cancellation because of no payment of annual fees