JP3056525B2 - 肺の界面活性タンパク質の単離および精製 - Google Patents

肺の界面活性タンパク質の単離および精製

Info

Publication number
JP3056525B2
JP3056525B2 JP3512939A JP51293991A JP3056525B2 JP 3056525 B2 JP3056525 B2 JP 3056525B2 JP 3512939 A JP3512939 A JP 3512939A JP 51293991 A JP51293991 A JP 51293991A JP 3056525 B2 JP3056525 B2 JP 3056525B2
Authority
JP
Japan
Prior art keywords
rsp
fusion protein
mixture
inclusion body
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP3512939A
Other languages
English (en)
Japanese (ja)
Other versions
JPH05502888A (ja
Inventor
ジェイ． ベンソン，ブラッドリー
バックリー，ダグラス
レシカー，デイビッド
ナイドゥ，エイシャ
バプティ シルバネス，カティ
Original Assignee
バイク グルデン ロンバーグ ケミッシェ ファブリク ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by バイク グルデン ロンバーグ ケミッシェ ファブリク ゲーエムベーハー filed Critical バイク グルデン ロンバーグ ケミッシェ ファブリク ゲーエムベーハー
Publication of JPH05502888A publication Critical patent/JPH05502888A/ja
Application granted granted Critical
Publication of JP3056525B2 publication Critical patent/JP3056525B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/785—Alveolar surfactant peptides; Pulmonary surfactant peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/848—Lungs

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
JP3512939A 1990-07-10 1991-07-10 肺の界面活性タンパク質の単離および精製 Expired - Lifetime JP3056525B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/551,524 US5258496A (en) 1990-07-10 1990-07-10 Isolation and purification of lung surfactant protein
US551,524 1990-07-10

Publications (2)

Publication Number Publication Date
JPH05502888A JPH05502888A (ja) 1993-05-20
JP3056525B2 true JP3056525B2 (ja) 2000-06-26

Family

ID=24201618

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3512939A Expired - Lifetime JP3056525B2 (ja) 1990-07-10 1991-07-10 肺の界面活性タンパク質の単離および精製

Country Status (10)

Country Link
US (2) US5258496A (enExample)
EP (1) EP0491033B1 (enExample)
JP (1) JP3056525B2 (enExample)
AT (1) ATE159534T1 (enExample)
CA (1) CA2065323C (enExample)
DE (1) DE69128022T2 (enExample)
DK (1) DK0491033T3 (enExample)
ES (1) ES2111570T3 (enExample)
GR (1) GR3025929T3 (enExample)
WO (1) WO1992000993A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0527788T3 (da) * 1990-04-04 2004-09-06 Chiron Corp Hepatitis C virus protease
US5258496A (en) * 1990-07-10 1993-11-02 Scios Nova Inc. Isolation and purification of lung surfactant protein
EP0652227B1 (en) * 1993-04-30 1998-02-25 Tokyo Tanabe Company Limited Method of purifying hydrophobic polypeptide
WO1996017942A1 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of peptides using recombinant fusion protein constructs
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
US6031075A (en) * 1996-02-05 2000-02-29 Children's Hospital Medical Center Mature alveolar SP-B and a process for producing the same
US6020307A (en) * 1997-04-25 2000-02-01 Ony, Inc. Compositions and methods for isolating lung surfactant hydrophobic proteins SP-B and SP-C
WO1997041155A2 (en) * 1996-04-26 1997-11-06 Ony, Inc. Compositions and methods for isolating lung surfactant hydrophobic proteins sp-b and sp-c
WO1997043313A1 (en) * 1996-05-15 1997-11-20 Byk Gulden Lomberg Chemische Fabrik Gmbh Process for separating and analyzing hydrophobic proteins using thin layer chromatography
CA2315888A1 (en) * 1997-12-23 1999-07-01 Eli Lilly And Company Beta-lipotropin and uses thereof
JPH11335392A (ja) * 1998-05-26 1999-12-07 Hsp Kenkyusho:Kk 可溶性タンパク質の回収方法
WO2008122089A1 (en) * 2007-04-05 2008-10-16 The University Of Queensland Method of purifying recombinant peptides by selective precipitation
WO2009035395A1 (en) 2007-09-14 2009-03-19 Marcus Larsson A lung-surfactant product

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0240550A4 (en) * 1985-09-26 1989-09-19 Genetics Inst TENSIO-ACTIVE PULMONARY PROTEINS.
US4918161A (en) * 1985-09-26 1990-04-17 Genetics Institute, Inc. Low molecular weight pulmonary surfactant proteins
US4882422A (en) * 1985-10-24 1989-11-21 Genetics Institute, Inc. Pulmonary surfactant proteins
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
JPS63503222A (ja) * 1986-05-06 1988-11-24 サイオス　ノバ　インコーポレイテッド 肺の疎水性界面活性物質に結合した分子量６，０００ダルトンのタンパク質及びその多量体
US5013720A (en) * 1986-05-06 1991-05-07 Abbott Laboratories SAP-6-Val proteins and methods
JPH01501282A (ja) * 1986-12-08 1989-05-11 ホイツトセツト，ジエフリー　エイ． 肺胞疎水性界面活性物質関連タンパク質
WO1988005820A1 (en) * 1987-01-29 1988-08-11 California Biotechnology, Inc. Recombinant alveolar surfactant protein
DE3855986T2 (de) * 1987-05-11 1998-02-05 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
JP2602968B2 (ja) * 1987-11-04 1997-04-23 カリフォルニア バイオテクノロジー インコーポレイテッド 肺胞の界面活性タンパク類
BR8807290A (pt) * 1987-11-12 1990-03-27 Unilever Nv Composicao de limpeza aquosa e tixotropica e processo para prepara-la
CH677879A5 (enExample) * 1988-07-22 1991-07-15 Pentapharm Ag
SE8803713D0 (sv) * 1988-10-18 1988-10-18 Kabigen Ab Biologically active lipoprotein and its use
DK0482097T3 (da) * 1989-07-11 2001-09-17 Genentech Inc Tensidpræparater og fremgangsmåder
US5258496A (en) * 1990-07-10 1993-11-02 Scios Nova Inc. Isolation and purification of lung surfactant protein

Also Published As

Publication number Publication date
US5258496A (en) 1993-11-02
GR3025929T3 (en) 1998-04-30
CA2065323C (en) 2003-06-10
CA2065323A1 (en) 1992-01-11
ATE159534T1 (de) 1997-11-15
HK1004336A1 (en) 1998-11-20
US5403915A (en) 1995-04-04
EP0491033A4 (enExample) 1994-03-30
WO1992000993A1 (en) 1992-01-23
EP0491033A1 (en) 1992-06-24
EP0491033B1 (en) 1997-10-22
DE69128022D1 (de) 1997-11-27
DK0491033T3 (da) 1998-07-20
JPH05502888A (ja) 1993-05-20
ES2111570T3 (es) 1998-03-16
DE69128022T2 (de) 1998-05-20

Similar Documents

Publication Publication Date Title
JP3056525B2 (ja) 肺の界面活性タンパク質の単離および精製
EP0518587B1 (en) A-C-B proinsulin, method of manufacturing and using same, and intermediates in insulin production
JP3333846B2 (ja) 組織因子経路阻害剤の製造法
JP2659530B2 (ja) ストレプトアビジン様ポリペプチドの製造
JP2766647B2 (ja) ペプチド類
EP0542863B1 (en) Expression of recombinant polypeptides with improved purification
US4656255A (en) Protein recovery
JP5291137B2 (ja) ８より上または５未満のｐＩを有するペプチドを産生するための方法
AU644105B2 (en) Production of biologically active insulin-like growth factor I from high expression host cell systems
JPH07265092A (ja) 正しく連結されたシスチン架橋を有するインシュリンを得る方法
EP0338634B1 (en) Viper venom polypeptides and genetic expression
JPH11503733A (ja) Ｉｇｆ−ｉの精製方法
EP1066328B1 (en) Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production
JP2517100B2 (ja) ヒト顆粒球コロニ―刺激因子活性を有する蛋白質の精製法
Gustafson et al. Renaturation and purification of human tissue factor pathway inhibitor expressed in recombinant E. coli
Peters et al. Biosynthesis of winter flounder antifreeze proprotein in E. coli
JP2798573B2 (ja) ヒト好中球エラスターゼ阻害活性を有する天然型ポリペプチドおよびそれを含有する医薬製剤
US20020164712A1 (en) Chimeric protein containing an intramolecular chaperone-like sequence
HK1004336B (en) Isolation and purification of lung surfactant protein
KR100535265B1 (ko) 융합 단백질로부터 목적 단백질을 분리하는 방법
JPH0556794A (ja) 生理活性ペプチドの製造法
JPH0568581A (ja) 生理活性ペプチドの製造法
CA2099967A1 (en) Process for recovering peptides expressed as fusion proteins
JP2000262293A (ja) 活性化蛋白質の製造法
JPS62248498A (ja) 新規生理活性ポリペプチド

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090414

Year of fee payment: 9

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090414

Year of fee payment: 9

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090414

Year of fee payment: 9

R360 Written notification for declining of transfer of rights

Free format text: JAPANESE INTERMEDIATE CODE: R360

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090414

Year of fee payment: 9

R370 Written measure of declining of transfer procedure

Free format text: JAPANESE INTERMEDIATE CODE: R370

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090414

Year of fee payment: 9

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20100414

Year of fee payment: 10

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110414

Year of fee payment: 11

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120414

Year of fee payment: 12

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120414

Year of fee payment: 12

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120414

Year of fee payment: 12

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

EXPY Cancellation because of completion of term
FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120414

Year of fee payment: 12