JP3055826B2 - Skin cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic composition having an excellent whitening effect, comprising an extract of a crude drug derived from specific legumes and lilies.

[0002]

2. Description of the Related Art
BACKGROUND ART Among skin cosmetics, whitening cosmetics are known as ones for obtaining cosmetic effects such as prevention of dark and dark skin, spots and freckles. This whitening cosmetic mainly contains ascorbic acid, glutathione, colloidal sulfur and the like, and has the above-mentioned beauty effect.

[0003] However, ascorbic acid is easily oxidized, so that not only is it difficult to expect a certain effect, but also there is a problem that the cosmetic itself may be discolored. Glutathione and colloidal sulfur also have the disadvantage of having a peculiar off-odor and precipitation depending on the dosage form.

Recently, attempts have been made to blend natural products such as crude drugs into cosmetics to obtain a whitening effect (JP-A-53-88333, JP-A-54-2344, and JP-A-54-2344). No. 57-163307, JP-A-60-104005
No. 6, Fragrance Journal Extra Edition No. 6 (198
6) P164-166 etc.). Although these crude drugs are expected to be useful because of their high safety, their whitening effect has not been sufficient.

[0005]

DISCLOSURE OF THE INVENTION In view of such circumstances, the present inventors have conducted intensive studies on the whitening effect and the like of crude drug extracts. As a result, the extract of crude drugs derived from specific legumes and lilies has high tyrosinase. It has an activity inhibiting effect, and it has been found that cosmetics containing these have excellent whitening effects, and also excellent safety and stability, and have completed the present invention.

[0006] That is, the present invention provides a skin cosmetic comprising one or more extracts of crude drugs selected from sea pirates, chicken blood wisteria, shellfish and yellow spirit. .

[0007] Crude drug extracts (hereinafter, referred to as seaweed skin, chicken blood wisteria, shellfish and yellow sperm) used in the skin cosmetic of the present invention.
There is no particular limitation on the method of preparing the “herbal extract”. However, preferable methods for extracting crude drugs include, for example, a method of extracting from various room temperature to heating using a suitable solvent, and the extraction solvent used for this is water; methyl alcohol, ethyl Lower polyhydric alcohols such as alcohols; liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol; lower alkyl esters such as ethyl acetate;
Examples thereof include hydrocarbons such as hexane; ethers such as diethyl ether, and one or more of them can be used. In particular, it is preferable to use water or a water-soluble solvent, particularly one or a mixture of two or more of water, ethyl alcohol, glycerin, and 1,3-butylene glycol.

The extraction conditions are as follows: a solvent is used in a volume ratio of 1 to 1000 times, particularly 5 to 100 times the volume of the crude drug, and at a temperature of 4 ° C. or more, especially 15 to 30 ° C. for 1 hour or more,
In particular, it is preferable to carry out for 1 to 3 days.

The crude drug extract obtained under the above-mentioned conditions may be added to the skin cosmetic of the present invention as it is in the form of an extracted solution. It can also be used separately.

[0010] The crude drug extracts of the present invention all have a whitening effect, but the extract of sea paulownia skin and chicken blood wisteria additionally has an effect of preventing skin aging and improving skin roughness by removing active oxygen. Have. Therefore, each of the crude drug extracts is blended into a skin cosmetic which is expected to have its effect.

In the skin cosmetic of the present invention, the content of the crude drug extract is 0.0001 to 10.000 in terms of dry solid content.
It is preferably about 0% by weight, particularly 0.01 to 5.0%.
A range of 0% by weight is preferred. The crude drug extract content is 0.
If it is less than 0001% by weight, the effect is not sufficiently exhibited,
When the content exceeds 10% by weight, the effect becomes almost constant.

[0012] The skin cosmetic of the present invention includes, in addition to the crude drug extract as an essential component, aqueous components, powders, surfactants, oils, humectants, alcohols, which are usually used in cosmetics, quasi-drugs and pharmaceuticals. pH adjuster, preservative, thickener, pigment,
It is prepared by appropriately blending a fragrance and the like as needed.

[0013] The form of the skin cosmetic of the present invention is not particularly limited, and lotions, emulsions, creams, packs, ointments, dispersions,
Various dosage forms such as a cleaning agent can be used.

Further, the skin cosmetic of the present invention may further contain a known drug if necessary. As this drug,
For example, agents having a whitening effect such as ascorbic acid, placenta extract, toki extract, mulberry bark extract, and aloe extract; anti-inflammatory agents such as glycyrrhetinic acid and its derivatives, indomethacin; Ultraviolet absorbers such as acids and their respective derivatives; antioxidants such as vitamin E, rosemary extract and tea extract; These drugs may be used alone or in combination of two or more.

[0015]

EXAMPLES The present invention will be described in more detail with reference to Test Examples and Examples, but the present invention is not limited thereto.

TEST EXAMPLE 1 Tyrosinase activity inhibition test: 20 parts by weight of dried crude drugs shown in Table 1 were mixed with ethyl alcohol or 5 parts by weight.
80 parts by weight of a 0% ethyl alcohol aqueous solution was added, and the mixture was extracted at room temperature with occasional stirring for 3 days and filtered to obtain each crude drug extract. Using each of these crude drug extracts as samples, the tyrosinase activity inhibition rate was measured by the following measurement method. Table 1 shows the results.

[Measurement method] Each sample 0.1 to 0.2 m
l of an enzyme solution [Sigma, 28,000 units of tyrosinase 10 mg in 0.1 M phosphate buffer (pH 6.8)
Dissolved in 20 ml], added 0.1 ml, and added 0.1
M phosphate buffer (pH 6.8) was added to make 4 ml, which was incubated at 25 ° C. for 10 minutes. The substrate solution [LD] previously kept at 25 ° C.
198.0 mg of OPA (Tokyo Kasei) dissolved in 100 ml of 0.1 M phosphate buffer (pH 6.8)] 1.0 m
1 was added and reacted for 10 minutes. Then 475 nm
Was measured for absorbance (OD _S ). Further, the absorbance (OD
_HE ) and absorbance (OD _B ) when no sample was added, and the inhibition rate of tyrosinase activity was calculated from the following equation. OD _B -OD _S tyrosinase activity inhibition rate (%) = ──────── × 100 OD B -OD HE

Table 1 添加 Sample solution addition amount Crude drug name Extraction dissolution Medium ──────────── 0.2 ml 0.1 ml ───────────────────────────────桐 Kai Tung Peel Ethyl Alcohol 51.4 (%) 20.2 (%) Chicken Blood Fuji Ethyl Alcohol 56.1 51.5 Shellfish Mother 50% Ethyl Al 62.5 41.6 Coal Aqueous Solution 143.2 〃 50% ethyl alcohol 55.5 45.4 coal aqueous solution ──────────────────────────────────

As is clear from the results in Table 1, the extracts of sea paulownia, chicken blood wisteria, shellfish, and yellow sperm used in the present invention suppress tyrosinase, reduce dopachrome production, and exhibit high whitening. Had an effect.

Test Example 2 Active oxygen removal effect test: Ethyl alcohol, 50% (V / V) aqueous solution of ethyl alcohol or 80 parts by weight of water was added to 20 parts by weight of each dried crude drug shown in Table 2 and Extraction was performed for 3 days at room temperature with occasional stirring, followed by filtration to obtain each crude drug extract. Each of these crude drug extracts was used as a sample, and the active oxygen removal rate was measured by the following measurement method. Table 2 shows the results.

[Measurement method] A substrate solution [3.0] was added to 2.4 ml of 0.05 M sodium carbonate buffer (pH 10.2).
mM xanthine (dissolved in 0.05M sodium carbonate buffer)], 0.1 ml, 0.1 mM 3.0 mM EDTA, 0.1 ml.
15% (W / V) bovine serum albumin 0.1 ml, 0.75
0.1 ml of mM Nitroble-tetrazolium and 0.1 ml of each sample were mixed and left at 25 ° C. for 10 minutes.
The reaction is started by adding 0.1 ml of an enzyme solution [xanthine oxidase solution (diluted with purified water to about 0.04 units / ml)]. After incubation at 25 ° C for 20 minutes, 6m
The reaction is stopped by adding 0.1 ml of M CuCl ₂ . Next, the absorbance (A) at 560 nm is measured. For the control, the absorbance (B) obtained by adding purified water instead of the sample,
The blank for each sample was 0.1 mM 6 mM CuCl _2.
After stopping the reaction by the addition of l, xanthine oxidase was added to the mixture.
The absorbance (C) to which 1 ml was added was measured, and the active oxygen removal rate was calculated from the following equation. B- (AC) Active oxygen removal rate (%) = ──────── × 100 B

Table 2 ───────────────────────────────── Sample solution Crude drug name Extracted solvent ──────────────── 5% solution 2% solution 1% solution ───────────────────────── Kai Tung Peel 50% Ethyl Al 61.5 38.5 26.9 Cole Aqueous Solution Chicken Blood Fuji 50% Ethyl Al 93.0 66.9 43.2 Chole Aqueous Solution Chicken Blood Fujimi Water 67.136 .2 19.4 ─────────────────────────────────

As is evident from the results in Table 2, the extract of sea paulownia skin and chicken blood wister used in the present invention removed active oxygen and had a high SOD-like antioxidant activity.

Example 1 Cosmetic water: <Composition> (% by weight) (1) Glycerin 5.0 (2) 1,3-butylene glycol 4.0 (3) Oleyl alcohol 0.1 (4) Polyoxyethylene sorbitan mono laurate ester (20EO) 1.5 (5) Polyoxyethylene lauryl ether (20EO) 0.5 (6) Ethyl alcohol 10.0 (7) Sorbitol 1.0 ( 8) Sea rind ethyl alcohol extract ^{* 1} 0.5 (9) Vitamin E 0.1 (10) Oxybenzone 0.2 (11) Preservatives qs (12) Flavor qs (13) Purified water balance ─ ──────────────────────────── Total 100.0 * 1: Sea-tung extract (Test Example 1) is dried solid About 8%

<Production Method> A. (3) to (6) and (9) to (12) are mixed and dissolved. B. Mix and dissolve (1), (2), (7), (8) and (13). C. Mix A and B to make them uniform.

Example 2 Cream: <Composition> (% by weight) (1) Beeswax 6.0 (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 37.0 (5) Glycerin monostearate 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene sorbitan mono laurate (20EO) 2.0 (8) Shellfish water extraction Liquid ^{* 2} 5.0 (9) Preservative appropriate amount (10) Flavor appropriate amount (11) 1,3-butylene glycol 5.0 (12) Purified water balance ─────────── ────────────────── Total 100.0 * 1: Contains about 8% dry solid content of shellfish extract (obtained in the same manner as in Test Example 1) What

<Production Method> A. Mix (1)-(7) and (9)-(10), heat to 70 ° C.
To keep. B. Mix (8), (11) and (12) and heat to maintain 70 ° C. C. Add A to B, emulsify uniformly, and cool to 30 ° C.

Example 3 Pack: <Composition> (% by weight) (1) Polyvinyl alcohol 15.0 (2) Sodium carboxymethylcellulose 5.0 (3) Propylene glycol 3.0 (4) Yellow spirit 1 , 3-butylene glycol extract ^{* 3} 2.5 (5) Ethyl alcohol 10.0 (6) Urocanic acid 0.1 (7) Preservative appropriate amount (8) Flavor appropriate amount (9) Purified water balance ─計 Total 100.0 * 3: Yellow sperm extract (obtained in the same manner as in Test Example 1) Containing about 1.0% as dry solids

<Production Method> A. (1) to (4), (6) and (9) are mixed, heated to 70 ° C. and dissolved with stirring. B. Mix (5), (7) and (8). C. Add B to A, mix and cool.

Example 4 Emulsion: <Composition> (% by weight) (1) Squalane 5.0 (2) Vaseline 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.5 8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) 1,3-butylene glycol 5.0 (7) Chicken blood wort ether extract ^{* 4} (as dry solid content) 0.1 (8) Ethyl alcohol 5.0 (9) Preservative appropriate amount (10) Flavor appropriate amount (11) Carboxyvinyl polymer (1.0% aqueous solution) 20.0 (12) Potassium hydroxide 0.1 (13) Purified water residue ───────────────────────────── Total 100.0 * 4: After extraction as in Test Example 1, What was obtained by drying.

<Production Method> A. Heat and mix (6) to (8) and (13), and maintain at 70 ° C. B. Heat and mix (1)-(5), (9) and (10) and keep at 70 ° C. C. Add A to B, mix, add (11) and mix uniformly, then add (12), emulsify uniformly and cool to 30 ° C.

Test Example 3 Use Effect Test: The whitening effect of the skin cosmetic of the present invention was tested by a use test. The usage test is
A panel of 20 women aged 30 to 40 years each, twice daily in the morning and evening, after washing the face, apply an appropriate amount of the test cosmetic on the face.
This was done by application over a week. The cream of Example 2 and the emulsion of Example 4 were used as test cosmetics, and the extract of shellfish from the cream of Example 2 was used as a control.
Except for (8), those corrected with purified water were used. Table 3 shows the results. The evaluation was performed according to the following criteria.

[Whitening effect] Effective: The spots and freckles are almost inconspicuous. Slightly effective: Simi Sobacas are less noticeable. Ineffective: No change.

Table 3 美 Whitening effect sample ──────── ────── Effective somewhat ineffective ク リ ー ム Cream of Example 2 Emulsion of Example 4 13 34 Reference item 0 4 16 ──────────────────────────────

As is clear from the results in Table 3, the use of the cream of Example 2 and the emulsion of Example 4 caused stains,
The freckles became less noticeable and an excellent whitening effect was obtained. In addition, as to the lotion of Example 1 and the pack of Example 3, substantially the same use test was carried out, and as a result, the same effect was obtained.

EXAMPLE 5 Except that 0.5% of a 50% ethyl alcohol aqueous solution extract of sea rind (containing about 7% dry solids of sea umbrella extract) was used as component (8). A lotion was obtained in the same manner as in Example 1.

EXAMPLE 6 Same as Example 2 except that 3.0% of a water extract of sea iris skin (containing about 6% dry solids of the sea umbrella extract) was used as the component (8). To give a cream.

Example 7 The procedure was carried out except that 3.0% of chicken blood wort 1,3-butylene glycol extract (containing 6% of chicken blood wort extract as dry solids) was used as component (4). A pack was obtained in the same manner as in Example 3.

Example 8 An emulsion was obtained in the same manner as in Example 4 except that 0.1% (as dry solids) chicken ether extract was used as the component (7).

Test Example 4 Use Effect Test: The skin cosmetic preparation of the present invention was tested for the effects of preventing skin aging and improving skin roughness by use tests. The use test was performed by applying the test cosmetics to the face appropriately for 12 weeks after washing the face twice daily in the morning and evening, using 20 women aged 30 to 55 as panels. As the test cosmetics, a control product prepared by removing the component (8) rind water extract from the cream of Example 6, the emulsion of Example 8, and the cream of Example 6 and correcting with purified water was used. Table 4 shows the results. The evaluation was performed according to the following criteria.

Skin aging prevention effect Effective: Skin abrasion and gloss were improved. Slightly effective: Slightly improved skin shine and gloss. Ineffective: No change from before use. Skin roughness improvement effect Efficacy: Skin roughness and roughness are improved. Slightly effective: Skin roughness and roughness are slightly improved. Ineffective: No change from before use.

Table 4 防止 Skin aging prevention effect Skin roughness improvement effect Sample ─────────── 有 Effective somewhat effective Ineffective Effective somewhat effective invalid 無ク リ ー ム Cream of Example 6 18 20 13 6 1 Emulsion of Example 8 14 4 2 15 5 0 Reference product 0 3 17 0 4 16 ──────────────────────────────────

As is clear from the results shown in Table 4, the cream of Example 6 and the emulsion of Example 8 were effective in preventing aging of the skin and for preventing rough skin.

[0044]

As described in detail above, the skin cosmetic of the present invention is excellent in whitening effect and can be widely applied to blackening of skin due to sunburn, prevention and improvement of spots and freckles. In particular, the skin cosmetic composition of the present invention containing the extract of sea paulownia and chicken blood wisteria exhibits excellent effects in preventing skin aging and skin roughness in addition to the above effects. Furthermore, since the skin cosmetic of the present invention is stable and safe, it can be used with confidence. that's all

Continuation of the front page (56) References JP-A-3-206019 (JP, A) JP-A-2-17118 (JP, A) JP-A-1-121210 (JP, A) JP-A-1-193211 (JP) , A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/48 A61K 7/00

Claims (1)

(57) [Claims] 1. A skin cosmetic comprising one or two or more extracts of crude drugs selected from sea pirates, chicken blood wisteria, shellfish and yellow spirit.