JP2961118B2 - ヒルジン変異体とその用途及び製造方法 - Google Patents

ヒルジン変異体とその用途及び製造方法

Info

Publication number
JP2961118B2
JP2961118B2 JP1057693A JP5769389A JP2961118B2 JP 2961118 B2 JP2961118 B2 JP 2961118B2 JP 1057693 A JP1057693 A JP 1057693A JP 5769389 A JP5769389 A JP 5769389A JP 2961118 B2 JP2961118 B2 JP 2961118B2
Authority
JP
Japan
Prior art keywords
hirudin
lys
mutant
yeast
hirudin mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP1057693A
Other languages
English (en)
Japanese (ja)
Other versions
JPH029390A (ja
Inventor
コトニー マイケル
デグリズ エリク
ロワソン ジェラール
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of JPH029390A publication Critical patent/JPH029390A/ja
Application granted granted Critical
Publication of JP2961118B2 publication Critical patent/JP2961118B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/855—Proteins from animals other than mammals or birds

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Removal Of Specific Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • External Artificial Organs (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
JP1057693A 1988-03-08 1989-03-08 ヒルジン変異体とその用途及び製造方法 Expired - Fee Related JP2961118B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8802925A FR2628429B1 (fr) 1988-03-08 1988-03-08 Variants de l'hirudine, leurs utilisations et les procedes pour les obtenir
FR8802925 1988-03-08

Publications (2)

Publication Number Publication Date
JPH029390A JPH029390A (ja) 1990-01-12
JP2961118B2 true JP2961118B2 (ja) 1999-10-12

Family

ID=9364012

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1057693A Expired - Fee Related JP2961118B2 (ja) 1988-03-08 1989-03-08 ヒルジン変異体とその用途及び製造方法

Country Status (10)

Country Link
US (1) US5087613A (enExample)
EP (1) EP0332523B1 (enExample)
JP (1) JP2961118B2 (enExample)
AT (1) ATE84571T1 (enExample)
CA (1) CA1341429C (enExample)
DE (1) DE68904330T2 (enExample)
ES (1) ES2036809T3 (enExample)
FR (1) FR2628429B1 (enExample)
GR (1) GR3007254T3 (enExample)
PT (1) PT89923B (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
US5268296A (en) * 1988-06-11 1993-12-07 Ciba-Geigy Corporation DNA vector and recombinant host cell for production of hirullin P6 and P18
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
FR2646437B1 (fr) * 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
JP3187044B2 (ja) * 1989-12-01 2001-07-11 ビーエーエスエフ アクチェンゲゼルシャフト ヒルジン突然変異蛋白質及びヒルジンポリアルキレングリコール複合体
US5672585A (en) * 1990-04-06 1997-09-30 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US5780303A (en) * 1990-04-06 1998-07-14 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US5612311A (en) 1990-04-06 1997-03-18 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US6521594B1 (en) 1990-04-06 2003-02-18 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
ZA915658B (en) * 1990-07-24 1992-05-27 Merrell Dow Pharma Analogs of hirudin having antiplatelet activity
CA2086243C (en) * 1990-07-24 2001-10-02 John L. Krstenansky Anticoagulant peptides
US5574012A (en) * 1990-07-24 1996-11-12 Merrell Pharmaceuticals Inc. Analogs of hirudin having anti-platelet activity
GB9023149D0 (en) * 1990-10-24 1990-12-05 British Bio Technology Proteins and nucleic acids
WO1993004082A1 (en) * 1991-08-20 1993-03-04 Baxter International Inc. Analogs of hirudin
US5837808A (en) * 1991-08-20 1998-11-17 Baxter International Inc. Analogs of hirudin
GB9209032D0 (en) * 1992-04-25 1992-06-10 Ciba Geigy Ag New peptide derivatives
ES2199963T3 (es) * 1993-06-11 2004-03-01 Merrell Pharmaceuticals Inc. Peptidos trifuncionales antitrombina y antiplaquetarios.
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
US8377863B2 (en) 2007-05-29 2013-02-19 Unigene Laboratories Inc. Peptide pharmaceutical for oral delivery
US9833411B2 (en) 2015-01-12 2017-12-05 Enteris Biopharma, Inc. Solid oral dosage forms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2567263B2 (ja) 1986-12-01 1996-12-25 トランスジーン ソシエテ アノニム ヒルジンの変異体、その使用及びその製造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3586333T2 (de) * 1984-03-27 1992-12-10 Transgene Sa Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin.
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2567263B2 (ja) 1986-12-01 1996-12-25 トランスジーン ソシエテ アノニム ヒルジンの変異体、その使用及びその製造方法

Also Published As

Publication number Publication date
US5087613A (en) 1992-02-11
CA1341429C (fr) 2003-05-27
DE68904330D1 (de) 1993-02-25
EP0332523A1 (fr) 1989-09-13
EP0332523B1 (fr) 1993-01-13
FR2628429B1 (fr) 1990-12-28
FR2628429A1 (fr) 1989-09-15
ATE84571T1 (de) 1993-01-15
JPH029390A (ja) 1990-01-12
ES2036809T3 (es) 1993-06-01
GR3007254T3 (enExample) 1993-07-30
DE68904330T2 (de) 1993-05-19
PT89923A (pt) 1989-11-10
PT89923B (pt) 1994-05-31

Similar Documents

Publication Publication Date Title
JP2961118B2 (ja) ヒルジン変異体とその用途及び製造方法
JP2515468B2 (ja) ヒト抗トロンビンｉｉｉ
Bock et al. Cloning and expression of the cDNA for human antithrom, bin III
US5824505A (en) Vectors, transformed cells and process for the preparation of hirudin
JP2610783B2 (ja) ポリクリングルプラスミノーゲン活性化因子をコードする遺伝子およびそれを含有するベクター
FI96966C (fi) Ilmentämiskasetti hirudiinivariantin tuottamiseksi hiivassa, sitä sisältävä plasmidi, sillä transformoitu hiivasolu ja menetelmä hirudiinivariantin valmistamiseksi hiivasolun avulla
US4517294A (en) Human antithrombin III
EP0138948B1 (en) Dna sequence encoding an exo-signal
JP2567263B2 (ja) ヒルジンの変異体、その使用及びその製造方法
US5705355A (en) Hirudin, pharmaceutical compositions comprising it and their use
EP0352089B1 (en) A modified human PSTI
AU685835B2 (en) High molecular weight desulphatohirudin
US5399684A (en) DNA sequences expressing mammalian alpha-1-antitrypsin
JPH0698004B2 (ja) Ｔｎｆ発現用新規プラスミド
AU603145B2 (en) Aminoglycoside phosphotransferase-proteinfusions
JPH01501996A (ja) 脱―表皮細胞成長因子プラスミノーゲンアクチベーター
EP0412526A2 (en) Expression vector for hirudin, hirudin fusion protein, transformed microorganism, and method for production of hirudin
JPH0246289A (ja) ４ｇ１２モノクローナル抗体をコードするｄｎａ塩基配列
HUT52559A (en) Process for production of proteins with trombolitic effect
JPH05304962A (ja) 腫瘍細胞増殖阻害因子の製造法
JPS6356290A (ja) 二本鎖ｄｎａフラグメントの製造方法
JPS62248498A (ja) 新規生理活性ポリペプチド

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees