JP2933280B2 - λＰ▲Ｌの下つき▼プロモーターとリボソーム結合部位の置換に便利なように工学処理した制限部位とを含有する発現ベクター、このベクターを含有するプラスミド、このプラスミドを含有する宿主、および関連方法 - Google Patents

λＰ▲Ｌの下つき▼プロモーターとリボソーム結合部位の置換に便利なように工学処理した制限部位とを含有する発現ベクター、このベクターを含有するプラスミド、このプラスミドを含有する宿主、および関連方法

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Publication number
JP2933280B2
JP2933280B2 JP60188317A JP18831785A JP2933280B2 JP 2933280 B2 JP2933280 B2 JP 2933280B2 JP 60188317 A JP60188317 A JP 60188317A JP 18831785 A JP18831785 A JP 18831785A JP 2933280 B2 JP2933280 B2 JP 2933280B2
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JP
Japan
Prior art keywords
plasmid
vector
gene
site
analog
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP60188317A
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English (en)
Japanese (ja)
Other versions
JPS61111693A (ja
Inventor
ハイム・アヴイヴ
ダニエル・バートフエルド
マリアン・ゴレツキ
ヤコブ・アール・ハートマン
ドヴ・カナー
アヴイグドル・レヴアノン
ヒラ・ロツカー―ギラデイ
アモス・ビー・オツペンハイム
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BAIO TEKUNOROJII JENERARU CORP
Original Assignee
BAIO TEKUNOROJII JENERARU CORP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/644,105 external-priority patent/US4742004A/en
Application filed by BAIO TEKUNOROJII JENERARU CORP filed Critical BAIO TEKUNOROJII JENERARU CORP
Publication of JPS61111693A publication Critical patent/JPS61111693A/ja
Application granted granted Critical
Publication of JP2933280B2 publication Critical patent/JP2933280B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP60188317A 1984-08-27 1985-08-27 λＰ▲Ｌの下つき▼プロモーターとリボソーム結合部位の置換に便利なように工学処理した制限部位とを含有する発現ベクター、このベクターを含有するプラスミド、このプラスミドを含有する宿主、および関連方法 Expired - Lifetime JP2933280B2 (ja)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US64511984A 1984-08-27 1984-08-27
US64467184A 1984-08-27 1984-08-27
US64455184A 1984-08-27 1984-08-27
US64424584A 1984-08-27 1984-08-27
US644671 1984-08-27
US644551 1984-08-27
US644245 1984-08-27
US645119 1984-08-27
US06/644,105 US4742004A (en) 1984-08-27 1984-08-27 Method for producing enzymatically active eucaryotic sod in bacteria
US644105 1984-08-27

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP6330418A Division JP2688180B2 (ja) 1984-08-27 1994-11-24 スーパーオキシドジスムターゼアナログの産生方法
JP6330419A Division JP2777074B2 (ja) 1984-08-27 1994-11-24 ポリペプチドおよびその製造方法

Publications (2)

Publication Number Publication Date
JPS61111693A JPS61111693A (ja) 1986-05-29
JP2933280B2 true JP2933280B2 (ja) 1999-08-09

Family

ID=27542038

Family Applications (4)

Application Number Title Priority Date Filing Date
JP60188317A Expired - Lifetime JP2933280B2 (ja) 1984-08-27 1985-08-27 λＰ▲Ｌの下つき▼プロモーターとリボソーム結合部位の置換に便利なように工学処理した制限部位とを含有する発現ベクター、このベクターを含有するプラスミド、このプラスミドを含有する宿主、および関連方法
JP6330418A Expired - Fee Related JP2688180B2 (ja) 1984-08-27 1994-11-24 スーパーオキシドジスムターゼアナログの産生方法
JP6330419A Expired - Lifetime JP2777074B2 (ja) 1984-08-27 1994-11-24 ポリペプチドおよびその製造方法
JP8095694A Pending JPH08283296A (ja) 1984-08-27 1996-04-17 ヒトアポリポプロテインｅアナログ及びヒトスーパーオキシドジスムターゼアナログ

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP6330418A Expired - Fee Related JP2688180B2 (ja) 1984-08-27 1994-11-24 スーパーオキシドジスムターゼアナログの産生方法
JP6330419A Expired - Lifetime JP2777074B2 (ja) 1984-08-27 1994-11-24 ポリペプチドおよびその製造方法
JP8095694A Pending JPH08283296A (ja) 1984-08-27 1996-04-17 ヒトアポリポプロテインｅアナログ及びヒトスーパーオキシドジスムターゼアナログ

Country Status (15)

Country Link
EP (2) EP0483113B1 (https=)
JP (4) JP2933280B2 (https=)
AT (2) ATE79407T1 (https=)
AU (1) AU603331B2 (https=)
CA (2) CA1340597C (https=)
DE (2) DE3588216T2 (https=)
DK (1) DK384185A (https=)
GR (1) GR852030B (https=)
HK (1) HK151695A (https=)
HU (2) HU215160B (https=)
IE (1) IE61141B1 (https=)
IL (3) IL110039A (https=)
MY (1) MY130321A (https=)
NZ (1) NZ213154A (https=)
PT (1) PT81025B (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU582536B2 (en) * 1983-04-29 1989-04-06 Yeda Research And Development Co. Ltd. Human superoxide dismutase cdna
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
EP0340805B1 (en) * 1983-10-03 1997-07-16 Chiron Corporation Superoxide dismutase and expression in microorganisms
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JP2594535B2 (ja) * 1984-11-30 1997-03-26 サントリー株式会社 高発現ベクターおよびその利用
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
EP0239631A4 (en) * 1985-10-04 1989-01-12 Biotech Res Partners Ltd RECOMBINANT APOLIPOPROTEINS AND METHODS.
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
IL96136A (en) * 1989-11-07 2000-12-06 Bio Technology General Corp Use of hydrophobic chromatography in a method for purification of copper-zinc superoxide dismutase from cells containing same
WO1991007496A1 (en) * 1989-11-09 1991-05-30 Allied-Signal Inc. Cloning and expression of genes encoding for polypeptides comprised of one or more repeating amino acid sequences
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
GB9303988D0 (en) * 1993-02-26 1993-04-14 Health Lab Service Board Bi-functional expression systems
US6030810A (en) * 1997-02-26 2000-02-29 Delgado; Stephen Gregory Cloned tetrodotoxin-sensitive sodium channel α-subunit and a splice variant thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK143412C (da) * 1979-05-17 1982-05-24 Forenede Bryggerier As Fremgangsmaade til udvinding af cu,zn-superoxid-dismutase fra gaer
BR8101972A (pt) * 1980-06-06 1982-08-17 Biogen Nv Vetor, processo para sua producao, processo para produzir uma molecula recombinante de dna, e processo para produzir um polipeptideo
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
DE3482840D1 (de) * 1983-01-21 1990-09-06 Transgene Sa Expressionsvektoren und deren verwendung zur herstellung eines proteins mit menschlicher alpha-antitrypsin-aktivitaet.
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
DE3485284D1 (de) * 1983-10-03 1992-01-02 Chiron Corp Klonieren von superoxid-dismutase und expression in mikroorganismen.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ＤＮＡ−ＲＥＣＯＭＢＩＮＡＴＩＯＮ ＩＮＴＥＲＡＣＴＩＯＮＳ ＡＮＤ ＲＥＰＡＩＲ，Ｐｅｒｇａｍｏｎ Ｐｒｅｓｓ（１９８０−５−３０）Ｐ．１９９−２１０

Also Published As

Publication number Publication date
DK384185D0 (da) 1985-08-23
DE3586473T2 (de) 1993-02-04
ATE79407T1 (de) 1992-08-15
HU214977B (hu) 1998-08-28
IE61141B1 (en) 1994-10-05
JPH08283296A (ja) 1996-10-29
DE3588216T2 (de) 2000-02-03
JPS61111693A (ja) 1986-05-29
GR852030B (https=) 1985-12-30
HK151695A (en) 1995-09-29
EP0483113A3 (en) 1992-05-13
IL110039A0 (en) 1994-10-07
CA1340597C (en) 1999-06-22
AU603331B2 (en) 1990-11-15
DK384185A (da) 1986-02-28
EP0483113B1 (en) 1999-09-22
DE3586473D1 (de) 1992-09-17
IL110039A (en) 1996-12-05
EP0483113A2 (en) 1992-04-29
EP0173280A1 (en) 1986-03-05
IL108976A0 (en) 1994-06-24
JPH07222596A (ja) 1995-08-22
AU4667785A (en) 1986-03-06
HU215160B (hu) 1998-10-28
JP2777074B2 (ja) 1998-07-16
CA1340464C (en) 1999-03-23
HUT40697A (en) 1987-01-28
MY130321A (en) 2007-06-29
DE3588216D1 (de) 1999-10-28
IL108976A (en) 1996-12-05
PT81025A (en) 1985-09-01
JPH07236490A (ja) 1995-09-12
JP2688180B2 (ja) 1997-12-08
NZ213154A (en) 1988-11-29
IL76192A0 (en) 1985-12-31
IL76192A (en) 1996-12-05
EP0173280B1 (en) 1992-08-12
ATE184916T1 (de) 1999-10-15
PT81025B (pt) 1988-01-22
IE852093L (en) 1986-02-27

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