JP2649608B2 - 磁性粒子を含有する乾式化学試薬を使用するフィブリノーゲン分析（すなわち、試料中の凝固し得るフィブリノーゲンの濃度測定）を正確に、迅速にかつ簡単に行うためのフィブリノーゲン分析方法 - Google Patents
磁性粒子を含有する乾式化学試薬を使用するフィブリノーゲン分析（すなわち、試料中の凝固し得るフィブリノーゲンの濃度測定）を正確に、迅速にかつ簡単に行うためのフィブリノーゲン分析方法Info
- Publication number
- JP2649608B2 JP2649608B2 JP3513307A JP51330791A JP2649608B2 JP 2649608 B2 JP2649608 B2 JP 2649608B2 JP 3513307 A JP3513307 A JP 3513307A JP 51330791 A JP51330791 A JP 51330791A JP 2649608 B2 JP2649608 B2 JP 2649608B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- fibrinogen
- reaction chamber
- reagent
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108010049003 Fibrinogen Proteins 0.000 title claims abstract description 153
- 102000008946 Fibrinogen Human genes 0.000 title claims abstract description 153
- 229940012952 fibrinogen Drugs 0.000 title claims abstract description 153
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 127
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 54
- 238000004458 analytical method Methods 0.000 title claims description 65
- 238000006243 chemical reaction Methods 0.000 claims abstract description 113
- 238000000034 method Methods 0.000 claims abstract description 65
- 210000004369 blood Anatomy 0.000 claims abstract description 63
- 239000008280 blood Substances 0.000 claims abstract description 63
- 239000011159 matrix material Substances 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000012530 fluid Substances 0.000 claims abstract description 7
- 238000004891 communication Methods 0.000 claims abstract description 3
- 239000000523 sample Substances 0.000 claims description 153
- 239000002245 particle Substances 0.000 claims description 42
- 238000005345 coagulation Methods 0.000 claims description 35
- 230000015271 coagulation Effects 0.000 claims description 35
- 108090000190 Thrombin Proteins 0.000 claims description 29
- 229960004072 thrombin Drugs 0.000 claims description 29
- 230000010355 oscillation Effects 0.000 claims description 20
- 239000000872 buffer Substances 0.000 claims description 19
- 239000003998 snake venom Substances 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 238000012544 monitoring process Methods 0.000 claims description 14
- PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical group CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 claims description 13
- 108010000499 Thromboplastin Proteins 0.000 claims description 13
- 102000002262 Thromboplastin Human genes 0.000 claims description 13
- 108010027612 Batroxobin Proteins 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 241001358279 Malaya Species 0.000 claims description 7
- 241000271897 Viperidae Species 0.000 claims description 7
- 230000009471 action Effects 0.000 claims description 7
- 229940057326 agkistrodon contortrix venom Drugs 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 5
- 230000004044 response Effects 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 208000007536 Thrombosis Diseases 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 229960003766 thrombin (human) Drugs 0.000 claims description 2
- 239000002435 venom Substances 0.000 claims description 2
- 231100000611 venom Toxicity 0.000 claims description 2
- 210000001048 venom Anatomy 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims 3
- 239000004365 Protease Substances 0.000 claims 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
- 238000006116 polymerization reaction Methods 0.000 claims 2
- 239000000538 analytical sample Substances 0.000 claims 1
- 230000005540 biological transmission Effects 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000003534 oscillatory effect Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 description 25
- 230000035602 clotting Effects 0.000 description 23
- 206010053567 Coagulopathies Diseases 0.000 description 21
- 238000012216 screening Methods 0.000 description 9
- 239000003146 anticoagulant agent Substances 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 102000009123 Fibrin Human genes 0.000 description 7
- 108010073385 Fibrin Proteins 0.000 description 7
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 7
- 239000002202 Polyethylene glycol Substances 0.000 description 7
- 229950003499 fibrin Drugs 0.000 description 7
- 229920001223 polyethylene glycol Polymers 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 208000032843 Hemorrhage Diseases 0.000 description 6
- 208000034158 bleeding Diseases 0.000 description 6
- 230000000740 bleeding effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 238000002560 therapeutic procedure Methods 0.000 description 5
- 230000002537 thrombolytic effect Effects 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 108010094028 Prothrombin Proteins 0.000 description 3
- 102100027378 Prothrombin Human genes 0.000 description 3
- 208000010378 Pulmonary Embolism Diseases 0.000 description 3
- 108010023197 Streptokinase Proteins 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000004520 agglutination Effects 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 229940127219 anticoagulant drug Drugs 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 239000012470 diluted sample Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229940012957 plasmin Drugs 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229940039716 prothrombin Drugs 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 229960005202 streptokinase Drugs 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000003527 fibrinolytic agent Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000002101 lytic effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 241000271510 Agkistrodon contortrix Species 0.000 description 1
- 108010058207 Anistreplase Proteins 0.000 description 1
- 206010003178 Arterial thrombosis Diseases 0.000 description 1
- 101000772006 Bombus ignitus Venom serine protease Bi-VSP Proteins 0.000 description 1
- 241000271511 Bothrops atrox Species 0.000 description 1
- 241000271064 Calloselasma rhodostoma Species 0.000 description 1
- 206010014513 Embolism arterial Diseases 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108010001014 Plasminogen Activators Proteins 0.000 description 1
- 102000001938 Plasminogen Activators Human genes 0.000 description 1
- 206010051077 Post procedural haemorrhage Diseases 0.000 description 1
- 238000012274 Preoperative evaluation Methods 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 229960000983 anistreplase Drugs 0.000 description 1
- 230000002744 anti-aggregatory effect Effects 0.000 description 1
- 230000004397 blinking Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007820 coagulation assay Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000282 fibrinogen degradation product Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229940127126 plasminogen activator Drugs 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000013432 robust analysis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000002345 thrombinlike Effects 0.000 description 1
- 229960000103 thrombolytic agent Drugs 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 229960005356 urokinase Drugs 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/745—Assays involving non-enzymic blood coagulation factors
- G01N2333/75—Fibrin; Fibrinogen
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Centrifugal Separators (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55057090A | 1990-07-10 | 1990-07-10 | |
| US550,570 | 1990-07-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06500463A JPH06500463A (ja) | 1994-01-20 |
| JP2649608B2 true JP2649608B2 (ja) | 1997-09-03 |
Family
ID=24197737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3513307A Expired - Fee Related JP2649608B2 (ja) | 1990-07-10 | 1991-06-28 | 磁性粒子を含有する乾式化学試薬を使用するフィブリノーゲン分析（すなわち、試料中の凝固し得るフィブリノーゲンの濃度測定）を正確に、迅速にかつ簡単に行うためのフィブリノーゲン分析方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5350676A (enExample) |
| EP (2) | EP0867723A3 (enExample) |
| JP (1) | JP2649608B2 (enExample) |
| AT (1) | ATE174633T1 (enExample) |
| AU (1) | AU660624B2 (enExample) |
| CA (1) | CA2087033C (enExample) |
| DE (1) | DE69130644T2 (enExample) |
| ES (1) | ES2128321T3 (enExample) |
| TW (1) | TW221493B (enExample) |
| WO (1) | WO1992001065A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008224687A (ja) * | 1998-06-25 | 2008-09-25 | Cardiovascular Diagnostics Inc | エカリンと磁性粒子を含む乾式化学試薬を用いてフィブリノーゲンアッセイを行う方法 |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114135A (en) * | 1991-11-08 | 2000-09-05 | Goldstein; Sheldon | Multiple coagulation test system and method of using a multiple coagulation test system |
| DK203191D0 (da) * | 1991-12-19 | 1991-12-19 | Novo Nordisk As | Fremgangsmaade og apparat til bestemmelse af relevante blodparametre |
| US5443959A (en) * | 1992-09-09 | 1995-08-22 | Tokuyama Corporation | Method of assaying fibrinogen, dry reagent therefor, and process for the preparation thereof |
| EP0587398B1 (en) * | 1992-09-09 | 1998-01-14 | Tokuyama Corporation | Method of assaying fibrinogen, dry reagent therefor, and process for the preparation thereof |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| WO1994019690A1 (en) * | 1993-02-17 | 1994-09-01 | Cardiovascular Diagnostics, Inc. | Dry chemistry cascade immunoassay and affinity assay |
| US5670329A (en) * | 1993-05-28 | 1997-09-23 | Cardiovascular Diagnostics, Inc. | Method and analytical system for performing fibrinogen assays accurately, rapidly and simply using a rotating magnetic field |
| US5522255A (en) | 1993-08-31 | 1996-06-04 | Boehringer Mannheim Corporation | Fluid dose, flow and coagulation sensor for medical instrument |
| EP0720439A4 (en) * | 1993-08-31 | 1997-09-24 | Boehringer Mannheim Corp | POWER SUPPLY CONTROL AND CONTROL FOR MEDICAL INSTRUMENT |
| US5841023A (en) * | 1993-08-31 | 1998-11-24 | Boehringer Mannheim Corporation | Magnet for medical instrument |
| US5526111A (en) * | 1993-08-31 | 1996-06-11 | Boehringer Mannheim Corporation | Method and apparatus for calculating a coagulation characteristic of a sample of blood a blood fraction or a control |
| US5502651A (en) * | 1994-05-02 | 1996-03-26 | Jackson; R. David | Potentiophotometric fibrinogen determination |
| US5504011A (en) * | 1994-10-21 | 1996-04-02 | International Technidyne Corporation | Portable test apparatus and associated method of performing a blood coagulation test |
| AU689143B2 (en) * | 1994-12-02 | 1998-03-26 | Cardiovascular Diagnostics, Inc. | Improved method and analytical system for performing fibrinogen assays accurately, rapidly and simply |
| US5567596A (en) * | 1994-12-29 | 1996-10-22 | Research Foundation Of State University Of New York | Rapid assay of activators and inhibitors of clotting |
| US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US6051378A (en) | 1996-03-04 | 2000-04-18 | Genetrace Systems Inc. | Methods of screening nucleic acids using mass spectrometry |
| US5965363A (en) | 1996-09-19 | 1999-10-12 | Genetrace Systems Inc. | Methods of preparing nucleic acids for mass spectrometric analysis |
| CA2270132A1 (en) | 1996-11-06 | 1998-05-14 | Sequenom, Inc. | Dna diagnostics based on mass spectrometry |
| US7285422B1 (en) | 1997-01-23 | 2007-10-23 | Sequenom, Inc. | Systems and methods for preparing and analyzing low volume analyte array elements |
| DE19782096T1 (de) | 1996-11-06 | 2000-03-23 | Sequenom Inc | Immobiliserung von Nucleinsäuren in hoher Dichte |
| DE69735445T2 (de) | 1996-12-10 | 2006-08-10 | Sequenom, Inc., San Diego | Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung |
| US5998224A (en) * | 1997-05-16 | 1999-12-07 | Abbott Laboratories | Magnetically assisted binding assays utilizing a magnetically responsive reagent |
| US6046051A (en) * | 1997-06-27 | 2000-04-04 | Hemosense, Inc. | Method and device for measuring blood coagulation or lysis by viscosity changes |
| HU222809B1 (hu) * | 1997-10-03 | 2003-10-28 | 77 Elektronika Műszeripari Kft. | Eljárás és készülék kémiai összetevőnek anyagmintából, különösen vér glükóztartalmának vérmintából történő meghatározásához |
| US6417004B1 (en) * | 1999-04-29 | 2002-07-09 | Helena Laboratories Corporation | Enhancing clot detection in activated clotting time and other fibrin endpoint based tests |
| US6294342B1 (en) | 1999-09-29 | 2001-09-25 | Abbott Laboratories | Magnetically assisted binding assays utilizing a magnetically responsive reagent |
| AU2001261020A1 (en) * | 2000-05-09 | 2001-11-20 | Pharmanetics Incorporated | Platelet function assay and reagent therefor |
| WO2001096607A2 (en) | 2000-06-13 | 2001-12-20 | The Trustees Of Boston University | Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing |
| KR100649342B1 (ko) | 2000-10-30 | 2006-11-27 | 시쿼넘, 인코포레이티드 | 기판 상으로 서브마이크로리터 볼륨들을 전달하기 위한 방법 및 장치 |
| US6759009B2 (en) | 2001-05-04 | 2004-07-06 | Portascience Incorporated | Method and device for clotting time assay |
| DE10140699A1 (de) * | 2001-08-24 | 2003-03-13 | Roche Diagnostics Gmbh | Anordnung und Verfahren zur Untersuchung der Fließfähigkeit einer physiologischen Flüssigprobe |
| US6902904B2 (en) * | 2001-08-27 | 2005-06-07 | Pharmanetics Incorporated | Coagulation assay reagents containing lanthanides |
| US6680177B2 (en) * | 2001-12-07 | 2004-01-20 | Cardiovascular Diagnostics, Inc. | Low molecular weight heparin assay, system and reagent therefor |
| DE10362199B4 (de) * | 2003-06-04 | 2009-09-10 | Stief, Thomas, Dr.med. | Nachweisverfahren für Fibrinogen |
| GB0313015D0 (en) * | 2003-06-06 | 2003-07-09 | Hall Effect Technologies Ltd | Method and device for analysing a liquid |
| EP1544596B1 (de) * | 2003-12-17 | 2016-11-23 | Boehringer Ingelheim microParts GmbH | Verfahren und Vorrichtung zur Bestimmung der Viskosität |
| US7439069B2 (en) | 2004-02-27 | 2008-10-21 | Nippoldt Douglas D | Blood coagulation test cartridge, system, and method |
| US7422905B2 (en) | 2004-02-27 | 2008-09-09 | Medtronic, Inc. | Blood coagulation test cartridge, system, and method |
| US7399637B2 (en) | 2004-04-19 | 2008-07-15 | Medtronic, Inc. | Blood coagulation test cartridge, system, and method |
| US9267167B2 (en) | 2004-06-28 | 2016-02-23 | Becton, Dickinson And Company | Dissolvable films and methods including the same |
| US8152305B2 (en) * | 2004-07-16 | 2012-04-10 | The University Of North Carolina At Chapel Hill | Methods, systems, and computer program products for full spectrum projection |
| US20060024776A1 (en) * | 2004-08-02 | 2006-02-02 | Mcmillian Ray | Magnetic particle capture of whole intact organisms from clinical samples |
| EP1845381A4 (en) * | 2005-06-23 | 2012-03-14 | Arkray Inc | ANALYTICAL TOOL |
| GB2438669A (en) * | 2006-03-07 | 2007-12-05 | Inverness Medical Switzerland | Sensing apparatus having a pair of coils and an oscillating member |
| US8490469B2 (en) * | 2007-02-22 | 2013-07-23 | The University Of North Carolina | Methods and systems for multiforce high throughput screening |
| US20080297169A1 (en) * | 2007-05-31 | 2008-12-04 | Greenquist Alfred C | Particle Fraction Determination of A Sample |
| WO2009039122A2 (en) | 2007-09-17 | 2009-03-26 | Sequenom, Inc. | Integrated robotic sample transfer device |
| EP2206063A1 (en) | 2007-10-16 | 2010-07-14 | Koninklijke Philips Electronics N.V. | Estimation of diagnostic markers |
| EP2215470B1 (en) * | 2007-11-02 | 2014-04-30 | Medplast S.A. | Method and apparatus for monitoring spatial fibrin clot formation |
| CN101983338B (zh) * | 2008-03-31 | 2015-03-04 | 希森美康株式会社 | 凝血分析仪、凝血分析方法及计算机程序 |
| US20110229498A1 (en) | 2008-05-08 | 2011-09-22 | The Johns Hopkins University | Compositions and methods for modulating an immune response |
| US9720003B2 (en) | 2008-12-22 | 2017-08-01 | Koninklijke Philips Electronics N.V. | Assay for Troponin I using magnetic labels |
| WO2010122158A1 (en) * | 2009-04-23 | 2010-10-28 | Dublin City University | A lateral flow assay device for coagulation monitoring and method thereof |
| JP5511949B2 (ja) | 2009-06-25 | 2014-06-04 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル | 体液レオロジーを評価するための、作動され表面に付着したポストを使用するための方法及びシステム |
| DE102011001952B8 (de) * | 2011-04-11 | 2012-12-20 | Johann-Wolfgang-Goethe Universität Frankfurt am Main | Vorrichtung und Verfahren zur Bestimmung der Koagulationszeit von Blut |
| JP6035329B2 (ja) * | 2011-05-26 | 2016-11-30 | ザ ジェネラル ホスピタル コーポレイション | 光学的トロンボエラストグラフィーシステム及び血液凝固基準の評価方法 |
| EP2926115B1 (en) | 2012-11-30 | 2021-05-26 | The University of North Carolina At Chapel Hill | Methods and systems for determining physical properties of a specimen in a portable point of care diagnostic device |
| US11172888B2 (en) | 2012-12-19 | 2021-11-16 | The General Hospital Corporation | Optical blood-coagulation sensor |
| TWI499779B (zh) | 2013-07-04 | 2015-09-11 | Ind Tech Res Inst | 檢測晶片及其使用方法 |
| US10823743B1 (en) | 2013-10-28 | 2020-11-03 | Ifirst Medical Technologies, Inc. | Methods of measuring coagulation of a biological sample |
| KR20160073016A (ko) * | 2014-12-16 | 2016-06-24 | 삼성전자주식회사 | 광학분석용 구조체 및 광학분석용 구조체 제조용 잉크 조성물 |
| KR102094241B1 (ko) * | 2018-04-17 | 2020-03-27 | 고려대학교산학협력단 | 혈액 샘플 내 피브리노겐 농도 측정 방법 및 이를 위한 나노입자 |
| WO2020051761A1 (zh) * | 2018-09-11 | 2020-03-19 | 深圳迈瑞生物医疗电子股份有限公司 | 凝血分析仪及其检测样本的方法、存储介质 |
| CN109856410A (zh) * | 2019-02-15 | 2019-06-07 | 王振华 | 一种血凝测试装置 |
| WO2021154995A1 (en) | 2020-01-29 | 2021-08-05 | Ifirst Medical Technologies, Inc. | Medical analyzer and diagnostic sample profiler |
| CN113976192B (zh) * | 2021-08-23 | 2023-08-15 | 上海汉原生物科技有限公司 | 微球标记微流控芯片以及微球蛋白标记方法 |
| US20230314405A1 (en) * | 2022-03-30 | 2023-10-05 | Instrumentation Laboratory Company | Clinical decision support system |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE792113A (fr) * | 1971-12-13 | 1973-05-30 | Technicon Instr | Melangeur de liquides contenant des particules magnetiques |
| US3861197A (en) * | 1973-06-29 | 1975-01-21 | Technicon Instr | Method and apparatus for determining the viscosity of a liquid sample |
| US4756884A (en) * | 1985-08-05 | 1988-07-12 | Biotrack, Inc. | Capillary flow device |
| US4849340A (en) * | 1987-04-03 | 1989-07-18 | Cardiovascular Diagnostics, Inc. | Reaction system element and method for performing prothrombin time assay |
| US5110727A (en) * | 1987-04-03 | 1992-05-05 | Cardiovascular Diagnostics, Inc. | Method for performing coagulation assays accurately, rapidly and simply, using dry chemical reagents and paramagnetic particles |
| US5156974A (en) * | 1988-05-27 | 1992-10-20 | Biodata Corporation | Method for determining the fibrinogen level of a blood sample |
-
1990
- 1990-07-31 TW TW079106328A patent/TW221493B/zh active
-
1991
- 1991-06-28 JP JP3513307A patent/JP2649608B2/ja not_active Expired - Fee Related
- 1991-06-28 DE DE69130644T patent/DE69130644T2/de not_active Expired - Fee Related
- 1991-06-28 AT AT91914171T patent/ATE174633T1/de not_active IP Right Cessation
- 1991-06-28 AU AU83119/91A patent/AU660624B2/en not_active Ceased
- 1991-06-28 ES ES91914171T patent/ES2128321T3/es not_active Expired - Lifetime
- 1991-06-28 CA CA002087033A patent/CA2087033C/en not_active Expired - Fee Related
- 1991-06-28 WO PCT/US1991/004472 patent/WO1992001065A1/en not_active Ceased
- 1991-06-28 EP EP98201760A patent/EP0867723A3/en not_active Withdrawn
- 1991-06-28 EP EP91914171A patent/EP0538393B1/en not_active Expired - Lifetime
-
1993
- 1993-05-17 US US08/062,334 patent/US5350676A/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008224687A (ja) * | 1998-06-25 | 2008-09-25 | Cardiovascular Diagnostics Inc | エカリンと磁性粒子を含む乾式化学試薬を用いてフィブリノーゲンアッセイを行う方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0538393A4 (enExample) | 1994-01-26 |
| CA2087033A1 (en) | 1992-01-11 |
| ES2128321T3 (es) | 1999-05-16 |
| EP0867723A2 (en) | 1998-09-30 |
| TW221493B (enExample) | 1994-03-01 |
| EP0538393A1 (en) | 1993-04-28 |
| DE69130644D1 (de) | 1999-01-28 |
| ATE174633T1 (de) | 1999-01-15 |
| WO1992001065A1 (en) | 1992-01-23 |
| DE69130644T2 (de) | 1999-07-29 |
| JPH06500463A (ja) | 1994-01-20 |
| US5350676A (en) | 1994-09-27 |
| CA2087033C (en) | 2003-06-24 |
| EP0538393B1 (en) | 1998-12-16 |
| EP0867723A3 (en) | 2003-10-08 |
| AU660624B2 (en) | 1995-07-06 |
| AU8311991A (en) | 1992-02-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2649608B2 (ja) | 磁性粒子を含有する乾式化学試薬を使用するフィブリノーゲン分析（すなわち、試料中の凝固し得るフィブリノーゲンの濃度測定）を正確に、迅速にかつ簡単に行うためのフィブリノーゲン分析方法 | |
| KR100351602B1 (ko) | 정확,신속및간결한피브리노겐분석을위한개선된방법및분석시스템 | |
| AU763074B2 (en) | Methods for performing fibrinogen assays using dry chemical reagents containing ecarin and magnetic particles | |
| EP0418235B1 (en) | Coagulation assay systems which utilize paramagnetic particles | |
| JP3392871B2 (ja) | 流体試料の粘性変化を分析するための装置およびその分析方法 | |
| CA2492486A1 (en) | Photometric determination of coagulation time in undiluted whole blood | |
| US20030044871A1 (en) | Coagulation assay reagents containing lanthanides and a protein C assay using such a lanthanide-containing reagent | |
| CN106999932A (zh) | 用于流体样本中的凝结测定的具有流体结的盒设备 | |
| US6759009B2 (en) | Method and device for clotting time assay | |
| JPS58172537A (ja) | 光散乱測定装置 | |
| JPH06209794A (ja) | フィブリノゲンの定量方法 | |
| KR0135782B1 (ko) | 건성 시약 및 상자성 입자를 사용하는 정확, 신속 및 간결한 응집 분석법 | |
| Olson | Beverly J. Pennell | |
| MXPA00012813A (en) | Methods for performing fibrinogen assays using dry chemical reagents containing ecarin and magnetic particles | |
| JPS58173455A (ja) | 光散乱測定方法 | |
| Laffan et al. | 16 Investigation of haemostasis | |
| IL92191A (en) | Coagulation assay using dry chemical reagents and paramagnetic particles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TRDD | Decision of grant or rejection written | ||
| R150 | Certificate of patent or registration of utility model |
Ref document number: 2649608 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |