JP2532788C - - Google Patents
Info
- Publication number
- JP2532788C JP2532788C JP2532788C JP 2532788 C JP2532788 C JP 2532788C JP 2532788 C JP2532788 C JP 2532788C
- Authority
- JP
- Japan
- Prior art keywords
- particles
- immunoreactive
- species
- kit
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002245 particle Substances 0.000 claims description 72
- 239000000463 material Substances 0.000 claims description 57
- 239000000126 substance Substances 0.000 claims description 45
- 238000001514 detection method Methods 0.000 claims description 43
- 241000894007 species Species 0.000 claims description 43
- 108090001123 antibodies Proteins 0.000 claims description 40
- 102000004965 antibodies Human genes 0.000 claims description 40
- 239000012491 analyte Substances 0.000 claims description 28
- 239000000969 carrier Substances 0.000 claims description 27
- 239000000047 product Substances 0.000 claims description 23
- 239000007795 chemical reaction product Substances 0.000 claims description 20
- 239000002184 metal Substances 0.000 claims description 18
- 229910052751 metal Inorganic materials 0.000 claims description 18
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 17
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 15
- 229960002685 biotin Drugs 0.000 claims description 15
- 235000020958 biotin Nutrition 0.000 claims description 15
- 239000011616 biotin Substances 0.000 claims description 15
- 230000001809 detectable Effects 0.000 claims description 13
- 108090001008 Avidin Proteins 0.000 claims description 12
- 210000002700 Urine Anatomy 0.000 claims description 11
- 239000000427 antigen Substances 0.000 claims description 10
- 102000038129 antigens Human genes 0.000 claims description 10
- 108091007172 antigens Proteins 0.000 claims description 10
- 239000000084 colloidal system Substances 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 9
- 210000001736 Capillaries Anatomy 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000011358 absorbing material Substances 0.000 claims description 6
- 238000003018 immunoassay Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 230000002829 reduced Effects 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims 9
- 238000003541 multi-stage reaction Methods 0.000 claims 5
- 239000002250 absorbent Substances 0.000 claims 4
- 230000002745 absorbent Effects 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 238000005755 formation reaction Methods 0.000 claims 2
- 239000012141 concentrate Substances 0.000 claims 1
- 238000009792 diffusion process Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 229920000126 Latex Polymers 0.000 description 21
- 239000004816 latex Substances 0.000 description 21
- 238000002372 labelling Methods 0.000 description 16
- 239000000523 sample Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- 108010090804 Streptavidin Proteins 0.000 description 13
- 238000004166 bioassay Methods 0.000 description 11
- 239000011162 core material Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 239000000376 reactant Substances 0.000 description 6
- 230000035935 pregnancy Effects 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 229940098773 Bovine Serum Albumin Drugs 0.000 description 3
- 108091003117 Bovine Serum Albumin Proteins 0.000 description 3
- 229940033663 Thimerosal Drugs 0.000 description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L Thiomersal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 3
- FPKOPBFLPLFWAD-UHFFFAOYSA-N Trinitrotoluene Chemical compound CC1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1[N+]([O-])=O FPKOPBFLPLFWAD-UHFFFAOYSA-N 0.000 description 3
- 150000007942 carboxylates Chemical class 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M isothiocyanate Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 3
- LBCZOTMMGHGTPH-UHFFFAOYSA-N 2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCO)C=C1 LBCZOTMMGHGTPH-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L disodium;2-[2-[carboxylatomethyl(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- -1 for example Substances 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- UVGHPGOONBRLCX-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoate Chemical compound S1CC2NC(=O)NC2C1CCCCC(=O)NCCCCCC(=O)ON1C(=O)CCC1=O UVGHPGOONBRLCX-UHFFFAOYSA-N 0.000 description 1
- JYCQQPHGFMYQCF-UHFFFAOYSA-N 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1 JYCQQPHGFMYQCF-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 210000004369 Blood Anatomy 0.000 description 1
- 229920002301 Cellulose acetate Polymers 0.000 description 1
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 229940088597 Hormone Drugs 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 206010061372 Streptococcal infection Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000890 antigenic Effects 0.000 description 1
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide Chemical compound [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007374 clinical diagnostic method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012612 commercial material Substances 0.000 description 1
- 230000002860 competitive Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 201000009910 diseases by infectious agent Diseases 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- ZBKIUFWVEIBQRT-UHFFFAOYSA-N gold(1+) Chemical compound [Au+] ZBKIUFWVEIBQRT-UHFFFAOYSA-N 0.000 description 1
- 230000003054 hormonal Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 108010045030 monoclonal antibodies Proteins 0.000 description 1
- 102000005614 monoclonal antibodies Human genes 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 230000000149 penetrating Effects 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 108091008117 polyclonal antibodies Proteins 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002285 radioactive Effects 0.000 description 1
- 230000000630 rising Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- BZKBCQXYZZXSCO-UHFFFAOYSA-N sodium hydride Inorganic materials [H-].[Na+] BZKBCQXYZZXSCO-UHFFFAOYSA-N 0.000 description 1
- 230000004936 stimulating Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
Family
ID=
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2532788B2 (ja) | 免疫学的検定を行うための装置と方法 | |
| JP2537873B2 (ja) | 金ゾル試薬の調整方法 | |
| EP0823972B1 (de) | Diagnostische vorrichtung und verfahren | |
| EP2126573B1 (de) | Diagnostische nachweisvorrichtung | |
| JP3125788B2 (ja) | 着色粒子イムノアッセイ用の試験装置および方法 | |
| EP0306772B1 (de) | Gerät für die laterale immunochromatographische Bestimmung | |
| CA1334931C (en) | Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite | |
| US4956302A (en) | Lateral flow chromatographic binding assay device | |
| JP5855572B2 (ja) | サンプル圧縮機を用いた複数面の側方流動アッセイ | |
| JP5173808B2 (ja) | 横向き移動でのイムノクロマトの手段による検体アッセイ | |
| JP3005303B2 (ja) | 測定装置 | |
| JPH04501760A (ja) | 流体中の反応性リガンドを定性的ないし定量的に迅速測定する装置及び方法 | |
| EP0258963A2 (de) | Immunoassay mit Verwendung von kolloidalem Gold | |
| JP2009517632A (ja) | 改善された標的リガンドの検出 | |
| EP0660932A1 (de) | Verfahren zur herstellung von testelementen | |
| CA2570383C (en) | Filter device, the method, kit and use thereof | |
| US20050277203A1 (en) | Immunochemical filter device and methods for use thereof | |
| JP2000055918A (ja) | 抗体アッセイ装置 | |
| JP2532788C (de) | ||
| WO2008071340A1 (en) | Antigen detection method involving an oligonucleotide enhanced colloidal gold signal |