JP2024512943A - 連続的なプライマー伸長による空間的なマッピング - Google Patents

連続的なプライマー伸長による空間的なマッピング Download PDF

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Publication number
JP2024512943A
JP2024512943A JP2023557789A JP2023557789A JP2024512943A JP 2024512943 A JP2024512943 A JP 2024512943A JP 2023557789 A JP2023557789 A JP 2023557789A JP 2023557789 A JP2023557789 A JP 2023557789A JP 2024512943 A JP2024512943 A JP 2024512943A
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sequence
barcoded
particles
nucleic acid
extension product
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JP2024512943A5 (https=
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フレドリクソン，サイモン
カールソン，フィリップ
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ピクセルゲン テクノロジーズ エービー
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Application filed by ピクセルゲン テクノロジーズ エービー filed Critical ピクセルゲン テクノロジーズ エービー
Publication of JP2024512943A publication Critical patent/JP2024512943A/ja
Publication of JP2024512943A5 publication Critical patent/JP2024512943A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Analogue/Digital Conversion (AREA)
  • Arrangements For Transmission Of Measured Signals (AREA)
JP2023557789A 2021-03-30 2022-03-29 連続的なプライマー伸長による空間的なマッピング Pending JP2024512943A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163168132P 2021-03-30 2021-03-30
US63/168,132 2021-03-30
PCT/IB2022/052862 WO2022208327A1 (en) 2021-03-30 2022-03-29 Spatial mapping by serial primer extension

Publications (2)

Publication Number Publication Date
JP2024512943A true JP2024512943A (ja) 2024-03-21
JP2024512943A5 JP2024512943A5 (https=) 2025-04-04

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ID=81327110

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JP2023557789A Pending JP2024512943A (ja) 2021-03-30 2022-03-29 連続的なプライマー伸長による空間的なマッピング

Country Status (7)

Country Link
US (2) US12123050B2 (https=)
EP (2) EP4303315B1 (https=)
JP (1) JP2024512943A (https=)
CN (1) CN117396612B (https=)
AU (1) AU2022247468A1 (https=)
CA (1) CA3213800A1 (https=)
WO (1) WO2022208327A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4555322B1 (en) 2022-09-27 2025-10-22 Pixelgen Technologies Ab Method for fixing primary antibodies to a biological sample
WO2024154075A1 (en) 2023-01-18 2024-07-25 Pixelgen Technologies Ab Gap-fill ligation and primer extension reactions employing exonuclease-resistant or capture moiety means
WO2024241162A1 (en) 2023-05-25 2024-11-28 Pixelgen Technologies Ab Peptide for site-specific antibody conjugation and method of use

Citations (4)

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JP2007525174A (ja) * 2003-04-18 2007-09-06 ベクトン・ディキンソン・アンド・カンパニー 免疫増幅
WO2015047186A1 (en) * 2013-09-30 2015-04-02 Di Wu Methods to profile molecular complexes by using proximity bar-coding
JP2022513561A (ja) * 2018-11-30 2022-02-09 イルミナ インコーポレイテッド 単一アッセイを使用した複数の分析物の分析
JP2022554421A (ja) * 2019-10-28 2022-12-28 ピクセルゲン テクノロジーズ エービー ローリングサークル増幅産物をマッピングするための方法

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US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
WO2012057689A1 (en) 2010-10-29 2012-05-03 Ge Healthcare Bio-Sciences Ab Proximity ligation technology for western blot applications
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
GB201201547D0 (en) * 2012-01-30 2012-03-14 Olink Ab Method and product
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
GB2539675B (en) * 2015-06-23 2017-11-22 Cs Genetics Ltd Libraries of multimeric barcoding reagents and kits thereof for labelling nucleic acids for sequencing
CA3176469A1 (en) * 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
WO2017044893A1 (en) 2015-09-11 2017-03-16 The Broad Institute, Inc. Dna microscopy
US11352667B2 (en) * 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US11549135B2 (en) * 2018-09-14 2023-01-10 The Broad Institute, Inc. Oligonucleotide-coupled antibodies for single cell or single complex protein measurements
AU2020357801A1 (en) * 2019-09-30 2022-04-14 Yale University Deterministic Barcoding for spatial omics sequencing

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JP2007525174A (ja) * 2003-04-18 2007-09-06 ベクトン・ディキンソン・アンド・カンパニー 免疫増幅
WO2015047186A1 (en) * 2013-09-30 2015-04-02 Di Wu Methods to profile molecular complexes by using proximity bar-coding
JP2022513561A (ja) * 2018-11-30 2022-02-09 イルミナ インコーポレイテッド 単一アッセイを使用した複数の分析物の分析
JP2022554421A (ja) * 2019-10-28 2022-12-28 ピクセルゲン テクノロジーズ エービー ローリングサークル増幅産物をマッピングするための方法

Non-Patent Citations (2)

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Title
GREENWOOD, CHRISTINA ET AL.: ""Proximity assays for sensitive quantification of proteins"", BIOMOLECULAR DETECTION AND QUANTIFICATION, vol. 4, JPN6024044069, 2015, pages 10 - 16, ISSN: 0005822551 *
WU, DI ET AL.: ""Profiling surface proteins on individual exosomes using a proximity barcoding assay"", NATURE COMMUNICATIONS, vol. Vol. 10, Article 3854, JPN6024044068, 26 August 2019 (2019-08-26), ISSN: 0005822552 *

Also Published As

Publication number Publication date
EP4303315B1 (en) 2024-11-06
US12123050B2 (en) 2024-10-22
US12416040B2 (en) 2025-09-16
AU2022247468A9 (en) 2024-02-22
EP4298240A1 (en) 2024-01-03
US20240175078A1 (en) 2024-05-30
CN117396612A (zh) 2024-01-12
EP4298240B1 (en) 2024-06-26
WO2022208327A1 (en) 2022-10-06
US20250011846A1 (en) 2025-01-09
CN117396612B (zh) 2025-02-28
EP4303315A3 (en) 2024-02-21
EP4303315A2 (en) 2024-01-10
AU2022247468A1 (en) 2023-10-05
CA3213800A1 (en) 2022-10-06
EP4298240C0 (en) 2024-06-26

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