CA3213800A1 - Spatial mapping by serial primer extension - Google Patents
Spatial mapping by serial primer extension Download PDFInfo
- Publication number
- CA3213800A1 CA3213800A1 CA3213800A CA3213800A CA3213800A1 CA 3213800 A1 CA3213800 A1 CA 3213800A1 CA 3213800 A CA3213800 A CA 3213800A CA 3213800 A CA3213800 A CA 3213800A CA 3213800 A1 CA3213800 A1 CA 3213800A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- barcoded
- nucleic acid
- particles
- extension products
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analogue/Digital Conversion (AREA)
- Arrangements For Transmission Of Measured Signals (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163168132P | 2021-03-30 | 2021-03-30 | |
| US63/168,132 | 2021-03-30 | ||
| PCT/IB2022/052862 WO2022208327A1 (en) | 2021-03-30 | 2022-03-29 | Spatial mapping by serial primer extension |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3213800A1 true CA3213800A1 (en) | 2022-10-06 |
Family
ID=81327110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3213800A Pending CA3213800A1 (en) | 2021-03-30 | 2022-03-29 | Spatial mapping by serial primer extension |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US12123050B2 (https=) |
| EP (2) | EP4303315B1 (https=) |
| JP (1) | JP2024512943A (https=) |
| CN (1) | CN117396612B (https=) |
| AU (1) | AU2022247468A1 (https=) |
| CA (1) | CA3213800A1 (https=) |
| WO (1) | WO2022208327A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4555322B1 (en) | 2022-09-27 | 2025-10-22 | Pixelgen Technologies Ab | Method for fixing primary antibodies to a biological sample |
| WO2024154075A1 (en) | 2023-01-18 | 2024-07-25 | Pixelgen Technologies Ab | Gap-fill ligation and primer extension reactions employing exonuclease-resistant or capture moiety means |
| WO2024241162A1 (en) | 2023-05-25 | 2024-11-28 | Pixelgen Technologies Ab | Peptide for site-specific antibody conjugation and method of use |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
| CN101410530B (zh) * | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| US8481698B2 (en) * | 2009-03-19 | 2013-07-09 | The President And Fellows Of Harvard College | Parallel proximity ligation event analysis |
| WO2012057689A1 (en) | 2010-10-29 | 2012-05-03 | Ge Healthcare Bio-Sciences Ab | Proximity ligation technology for western blot applications |
| WO2012106385A2 (en) | 2011-01-31 | 2012-08-09 | Apprise Bio, Inc. | Methods of identifying multiple epitopes in cells |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| GB201201547D0 (en) * | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| CN105745334B (zh) * | 2013-09-30 | 2019-12-10 | 吴迪 | 通过使用邻近条形编码分析分子复合物的概况的方法 |
| EP3058091B1 (en) | 2013-10-18 | 2020-03-25 | The Broad Institute, Inc. | Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing |
| US20150298091A1 (en) * | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US11639522B2 (en) | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| GB2539675B (en) * | 2015-06-23 | 2017-11-22 | Cs Genetics Ltd | Libraries of multimeric barcoding reagents and kits thereof for labelling nucleic acids for sequencing |
| CA3176469A1 (en) * | 2015-07-27 | 2017-02-02 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
| WO2017044893A1 (en) | 2015-09-11 | 2017-03-16 | The Broad Institute, Inc. | Dna microscopy |
| US11352667B2 (en) * | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US11549135B2 (en) * | 2018-09-14 | 2023-01-10 | The Broad Institute, Inc. | Oligonucleotide-coupled antibodies for single cell or single complex protein measurements |
| CN112867801B (zh) * | 2018-11-30 | 2024-07-12 | Illumina公司 | 使用单一测定分析多种分析物 |
| AU2020357801A1 (en) * | 2019-09-30 | 2022-04-14 | Yale University | Deterministic Barcoding for spatial omics sequencing |
| CN114616344B (zh) * | 2019-10-28 | 2026-03-31 | 普赛尔根技术股份公司 | 用于绘制滚环扩增产物图谱的方法 |
-
2022
- 2022-03-29 EP EP23201661.8A patent/EP4303315B1/en active Active
- 2022-03-29 JP JP2023557789A patent/JP2024512943A/ja active Pending
- 2022-03-29 CA CA3213800A patent/CA3213800A1/en active Pending
- 2022-03-29 US US18/283,172 patent/US12123050B2/en active Active
- 2022-03-29 WO PCT/IB2022/052862 patent/WO2022208327A1/en not_active Ceased
- 2022-03-29 CN CN202280038340.6A patent/CN117396612B/zh active Active
- 2022-03-29 EP EP22714926.7A patent/EP4298240B1/en active Active
- 2022-03-29 AU AU2022247468A patent/AU2022247468A1/en active Pending
-
2024
- 2024-09-17 US US18/888,001 patent/US12416040B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP4303315B1 (en) | 2024-11-06 |
| US12123050B2 (en) | 2024-10-22 |
| US12416040B2 (en) | 2025-09-16 |
| AU2022247468A9 (en) | 2024-02-22 |
| EP4298240A1 (en) | 2024-01-03 |
| US20240175078A1 (en) | 2024-05-30 |
| CN117396612A (zh) | 2024-01-12 |
| EP4298240B1 (en) | 2024-06-26 |
| WO2022208327A1 (en) | 2022-10-06 |
| US20250011846A1 (en) | 2025-01-09 |
| CN117396612B (zh) | 2025-02-28 |
| EP4303315A3 (en) | 2024-02-21 |
| EP4303315A2 (en) | 2024-01-10 |
| AU2022247468A1 (en) | 2023-10-05 |
| JP2024512943A (ja) | 2024-03-21 |
| EP4298240C0 (en) | 2024-06-26 |
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