JP2024512463A5 - - Google Patents

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Publication number
JP2024512463A5
JP2024512463A5 JP2023556903A JP2023556903A JP2024512463A5 JP 2024512463 A5 JP2024512463 A5 JP 2024512463A5 JP 2023556903 A JP2023556903 A JP 2023556903A JP 2023556903 A JP2023556903 A JP 2023556903A JP 2024512463 A5 JP2024512463 A5 JP 2024512463A5
Authority
JP
Japan
Prior art keywords
blocking
pool
oligonucleotides
blocking oligonucleotides
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023556903A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024512463A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2022/022663 external-priority patent/WO2022212589A1/en
Publication of JP2024512463A publication Critical patent/JP2024512463A/ja
Publication of JP2024512463A5 publication Critical patent/JP2024512463A5/ja
Pending legal-status Critical Current

Links

JP2023556903A 2021-03-31 2022-03-30 増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド Pending JP2024512463A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163169185P 2021-03-31 2021-03-31
US63/169,185 2021-03-31
PCT/US2022/022663 WO2022212589A1 (en) 2021-03-31 2022-03-30 Blocking oligonucleotides for the selective depletion of non-desirable fragments from amplified libraries

Publications (2)

Publication Number Publication Date
JP2024512463A JP2024512463A (ja) 2024-03-19
JP2024512463A5 true JP2024512463A5 (https=) 2025-04-07

Family

ID=81346581

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023556903A Pending JP2024512463A (ja) 2021-03-31 2022-03-30 増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド

Country Status (11)

Country Link
US (1) US20240191288A1 (https=)
EP (1) EP4314335A1 (https=)
JP (1) JP2024512463A (https=)
KR (1) KR20230163386A (https=)
CN (1) CN117098855A (https=)
AU (1) AU2022252302A1 (https=)
BR (1) BR112023019999A2 (https=)
CA (1) CA3213037A1 (https=)
IL (1) IL306060A (https=)
MX (1) MX2023011523A (https=)
WO (1) WO2022212589A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
WO2024220771A1 (en) * 2023-04-21 2024-10-24 Ohio State Innovation Foundation Rapid enzyme-free release of single-stranded dna from unmodified dsdna templates
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency
CN120060439B (zh) * 2025-02-27 2026-03-10 江苏硕世生物科技股份有限公司 一种基于封闭引物去人源核酸的测序文库构建方法及应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
JP5073967B2 (ja) 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
CA2877094A1 (en) * 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20140274729A1 (en) * 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
WO2017142989A1 (en) * 2016-02-17 2017-08-24 Admera Health LLC Nucleic acid preparation and analysis
CN110382708A (zh) * 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
EP3622089B1 (en) * 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
WO2020068559A1 (en) * 2018-09-25 2020-04-02 Qiagen Sciences, Llc Depleting unwanted rna species

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