IL306060A - Blocking oligonucleotides for selective depletion of unwanted fragments from amplified libraries - Google Patents
Blocking oligonucleotides for selective depletion of unwanted fragments from amplified librariesInfo
- Publication number
- IL306060A IL306060A IL306060A IL30606023A IL306060A IL 306060 A IL306060 A IL 306060A IL 306060 A IL306060 A IL 306060A IL 30606023 A IL30606023 A IL 30606023A IL 306060 A IL306060 A IL 306060A
- Authority
- IL
- Israel
- Prior art keywords
- blocking oligonucleotides
- selective depletion
- amplified libraries
- desirable fragments
- fragments
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 230000000903 blocking effect Effects 0.000 title 1
- 239000012634 fragment Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/117—Modifications characterised by incorporating modified base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163169185P | 2021-03-31 | 2021-03-31 | |
| PCT/US2022/022663 WO2022212589A1 (en) | 2021-03-31 | 2022-03-30 | Blocking oligonucleotides for the selective depletion of non-desirable fragments from amplified libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL306060A true IL306060A (en) | 2023-11-01 |
Family
ID=81346581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL306060A IL306060A (en) | 2021-03-31 | 2022-03-30 | Blocking oligonucleotides for selective depletion of unwanted fragments from amplified libraries |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20240191288A1 (https=) |
| EP (1) | EP4314335A1 (https=) |
| JP (1) | JP2024512463A (https=) |
| KR (1) | KR20230163386A (https=) |
| CN (1) | CN117098855A (https=) |
| AU (1) | AU2022252302A1 (https=) |
| BR (1) | BR112023019999A2 (https=) |
| CA (1) | CA3213037A1 (https=) |
| IL (1) | IL306060A (https=) |
| MX (1) | MX2023011523A (https=) |
| WO (1) | WO2022212589A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024137316A1 (en) * | 2022-12-23 | 2024-06-27 | Foundation Medicine, Inc. | Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs |
| WO2024220771A1 (en) * | 2023-04-21 | 2024-10-24 | Ohio State Innovation Foundation | Rapid enzyme-free release of single-stranded dna from unmodified dsdna templates |
| WO2025244841A1 (en) | 2024-05-23 | 2025-11-27 | Illumina, Inc. | Methods and systems for quantifying targeted enrichment efficiency |
| CN120060439B (zh) * | 2025-02-27 | 2026-03-10 | 江苏硕世生物科技股份有限公司 | 一种基于封闭引物去人源核酸的测序文库构建方法及应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| US6391592B1 (en) * | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| JP5073967B2 (ja) | 2006-05-30 | 2012-11-14 | 株式会社日立製作所 | 単一細胞の遺伝子発現定量方法 |
| CA2877094A1 (en) * | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20140274729A1 (en) * | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
| WO2017142989A1 (en) * | 2016-02-17 | 2017-08-24 | Admera Health LLC | Nucleic acid preparation and analysis |
| CN110382708A (zh) * | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| EP3622089B1 (en) * | 2017-05-08 | 2024-07-17 | Illumina, Inc. | Method for sequencing using universal short adapters for indexing of polynucleotide samples |
| WO2020068559A1 (en) * | 2018-09-25 | 2020-04-02 | Qiagen Sciences, Llc | Depleting unwanted rna species |
-
2022
- 2022-03-30 JP JP2023556903A patent/JP2024512463A/ja active Pending
- 2022-03-30 CA CA3213037A patent/CA3213037A1/en active Pending
- 2022-03-30 EP EP22718007.2A patent/EP4314335A1/en active Pending
- 2022-03-30 IL IL306060A patent/IL306060A/en unknown
- 2022-03-30 CN CN202280025253.7A patent/CN117098855A/zh active Pending
- 2022-03-30 US US18/285,222 patent/US20240191288A1/en active Pending
- 2022-03-30 AU AU2022252302A patent/AU2022252302A1/en not_active Abandoned
- 2022-03-30 BR BR112023019999A patent/BR112023019999A2/pt not_active Application Discontinuation
- 2022-03-30 WO PCT/US2022/022663 patent/WO2022212589A1/en not_active Ceased
- 2022-03-30 KR KR1020237032007A patent/KR20230163386A/ko active Pending
- 2022-03-30 MX MX2023011523A patent/MX2023011523A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN117098855A (zh) | 2023-11-21 |
| MX2023011523A (es) | 2023-10-06 |
| BR112023019999A2 (pt) | 2023-11-14 |
| KR20230163386A (ko) | 2023-11-30 |
| WO2022212589A1 (en) | 2022-10-06 |
| EP4314335A1 (en) | 2024-02-07 |
| AU2022252302A1 (en) | 2023-09-14 |
| JP2024512463A (ja) | 2024-03-19 |
| CA3213037A1 (en) | 2022-10-06 |
| US20240191288A1 (en) | 2024-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL306060A (en) | Blocking oligonucleotides for selective depletion of unwanted fragments from amplified libraries | |
| IL289290A (en) | Substituted benzyltriazole compounds for cbl-b inhibition, and other uses thereof | |
| SG11202103317XA (en) | Combinatorial cancer immunotherapy | |
| EP3781564A4 (en) | Compounds for the treatment of cancer | |
| SG11202100076RA (en) | Pyrimidine compounds and pharmaceutical compositions for preventing or treating cancers including the same | |
| SG10202007511WA (en) | Enclosure for gas detector | |
| IL316870A (en) | Compounds for targeted unloading of SMARCA2 | |
| WO2017001926A3 (en) | Therapeutic inhibitory compounds | |
| WO2017001936A3 (en) | Therapeutic inhibitory compounds | |
| IL285807A (en) | Immunotherapy for cancer treatment | |
| IL290177A (en) | History of 4,2,1-oxadiazol-5-one for cancer therapy | |
| WO2012051207A3 (en) | FRAGMENT OF SECRETED HEAT SHOCK PROTEIN-90ALPHA (Hsp90ALPHA) AS VACCINES OR EPITOPE FOR MONOCLONAL ANTIBODY DRUGS OR TARGET FOR SMALL MOLECULE DRUGS AGAINST A RANGE OF SOLID HUMAN TUMORS | |
| IL286872A (en) | Antisense oligonucleotides for the treatment of usher syndrome | |
| IL284266A (en) | History of (pyridin-2-yl)amine as an inhibitor of TGF-β in cell R1 (ALK5) for cancer treatment | |
| IL287901A (en) | A process for making clc-1 chloride channel inhibitors | |
| SG11202107906UA (en) | Metal chelator combination therapy for the treatment of cancer | |
| IL304751A (en) | A process for the detritylation of oligonucleotides | |
| SG11202104119PA (en) | Liner assembly, reaction chamber and semiconductor processing apparatus | |
| ZA202201166B (en) | Car t-cells against bcma for the treatment of multiple myeloma | |
| IL289201A (en) | Compounds for the treatment of cancer | |
| GB201909468D0 (en) | Compounds for treating cancer | |
| EP3794911A4 (en) | DISCHARGE CHAMBERS AND IONIZATION DEVICES, METHODS AND SYSTEMS USING THEM | |
| HK40106194A (en) | Blocking oligonucleotides for the selective depletion of non-desirable fragments from amplified libraries | |
| SG11202000515TA (en) | Faraday shield and reaction chamber | |
| PL3630754T3 (pl) | Związki izoindolinoacetylenowe do leczenia nowotworu |