JP2024512463A - 増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド - Google Patents

増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド Download PDF

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JP2024512463A
JP2024512463A JP2023556903A JP2023556903A JP2024512463A JP 2024512463 A JP2024512463 A JP 2024512463A JP 2023556903 A JP2023556903 A JP 2023556903A JP 2023556903 A JP2023556903 A JP 2023556903A JP 2024512463 A JP2024512463 A JP 2024512463A
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rna
blocking oligonucleotides
blocking
pcr
cdna
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JP2024512463A5 (https=
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ブラウン、コリン
シュルツァバーガー、サラ
エム グロス、スティーブン
バー、アンジェリカ
スノー、サマンサ
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イルミナ インコーポレイティッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

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  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2023556903A 2021-03-31 2022-03-30 増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド Pending JP2024512463A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163169185P 2021-03-31 2021-03-31
US63/169,185 2021-03-31
PCT/US2022/022663 WO2022212589A1 (en) 2021-03-31 2022-03-30 Blocking oligonucleotides for the selective depletion of non-desirable fragments from amplified libraries

Publications (2)

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JP2024512463A true JP2024512463A (ja) 2024-03-19
JP2024512463A5 JP2024512463A5 (https=) 2025-04-07

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JP2023556903A Pending JP2024512463A (ja) 2021-03-31 2022-03-30 増幅されたライブラリからの望ましくない断片の選択的枯渇のためのブロッキングオリゴヌクレオチド

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Country Link
US (1) US20240191288A1 (https=)
EP (1) EP4314335A1 (https=)
JP (1) JP2024512463A (https=)
KR (1) KR20230163386A (https=)
CN (1) CN117098855A (https=)
AU (1) AU2022252302A1 (https=)
BR (1) BR112023019999A2 (https=)
CA (1) CA3213037A1 (https=)
IL (1) IL306060A (https=)
MX (1) MX2023011523A (https=)
WO (1) WO2022212589A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
WO2024220771A1 (en) * 2023-04-21 2024-10-24 Ohio State Innovation Foundation Rapid enzyme-free release of single-stranded dna from unmodified dsdna templates
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency
CN120060439B (zh) * 2025-02-27 2026-03-10 江苏硕世生物科技股份有限公司 一种基于封闭引物去人源核酸的测序文库构建方法及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017142989A1 (en) * 2016-02-17 2017-08-24 Admera Health LLC Nucleic acid preparation and analysis
WO2020068559A1 (en) * 2018-09-25 2020-04-02 Qiagen Sciences, Llc Depleting unwanted rna species

Family Cites Families (12)

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
JP5073967B2 (ja) 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
CA2877094A1 (en) * 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20140274729A1 (en) * 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
CN110382708A (zh) * 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
EP3622089B1 (en) * 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017142989A1 (en) * 2016-02-17 2017-08-24 Admera Health LLC Nucleic acid preparation and analysis
WO2020068559A1 (en) * 2018-09-25 2020-04-02 Qiagen Sciences, Llc Depleting unwanted rna species

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Publication number Publication date
CN117098855A (zh) 2023-11-21
MX2023011523A (es) 2023-10-06
BR112023019999A2 (pt) 2023-11-14
KR20230163386A (ko) 2023-11-30
WO2022212589A1 (en) 2022-10-06
EP4314335A1 (en) 2024-02-07
AU2022252302A1 (en) 2023-09-14
CA3213037A1 (en) 2022-10-06
IL306060A (en) 2023-11-01
US20240191288A1 (en) 2024-06-13

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