JP2023550459A - 抗体構築物の発現技術 - Google Patents

抗体構築物の発現技術 Download PDF

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Publication number
JP2023550459A
JP2023550459A JP2023530543A JP2023530543A JP2023550459A JP 2023550459 A JP2023550459 A JP 2023550459A JP 2023530543 A JP2023530543 A JP 2023530543A JP 2023530543 A JP2023530543 A JP 2023530543A JP 2023550459 A JP2023550459 A JP 2023550459A
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Japan
Prior art keywords
heavy chain
antibody
peptide
nucleic acid
variable region
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JP2023530543A
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English (en)
Japanese (ja)
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JP2023550459A5 (enExample
Inventor
チェラー，ダッタナンダ
ヘスリング，マーティン
リッター，アネット
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ノバルティス アーゲー
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Publication of JP2023550459A publication Critical patent/JP2023550459A/ja
Publication of JP2023550459A5 publication Critical patent/JP2023550459A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/524—CH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2023530543A 2020-11-23 2021-11-22 抗体構築物の発現技術 Pending JP2023550459A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063117379P 2020-11-23 2020-11-23
US63/117,379 2020-11-23
PCT/IB2021/060796 WO2022107090A1 (en) 2020-11-23 2021-11-22 Expression technology for antibody constructs

Publications (2)

Publication Number Publication Date
JP2023550459A true JP2023550459A (ja) 2023-12-01
JP2023550459A5 JP2023550459A5 (enExample) 2024-11-29

Family

ID=78819584

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023530543A Pending JP2023550459A (ja) 2020-11-23 2021-11-22 抗体構築物の発現技術

Country Status (5)

Country Link
US (1) US20250282894A1 (enExample)
EP (1) EP4247857A1 (enExample)
JP (1) JP2023550459A (enExample)
CN (1) CN116615462A (enExample)
WO (1) WO2022107090A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117402253B (zh) * 2023-08-02 2024-08-23 南京大学 一种抗哇巴因的纳米抗体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017527522A (ja) * 2014-03-03 2017-09-21 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 二重特異性抗体及びその使用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8003106B2 (en) * 2002-03-13 2011-08-23 Kyowa Hakko Kirin Co., Ltd. Human monoclonal antibodies to influenza M2 protein and methods of making and using same
ES2546086T3 (es) 2007-12-21 2015-09-18 Novartis Ag Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana
PT3027646T (pt) 2013-07-31 2018-10-16 Novartis Ag Novos vetores e métodos de seleção de células hospedeiras eucarióticas
CR20160519A (es) * 2014-05-13 2017-01-27 The Trustees Of The Univ Of Pennysylvania Composiciones que comprenden avv que expresa constructos de anticuerpos duales y sus usos
US10144768B2 (en) 2015-12-04 2018-12-04 Novartis Ag Antibody cytokine engrafted compositions and methods of use for immunoregulation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017527522A (ja) * 2014-03-03 2017-09-21 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 二重特異性抗体及びその使用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HSIN-YI TUNG ET AL.: ""Selective Delivery of PEGylated Compounds to Tumor Cells by Anti-PEG Hybrid Antibodies"", MOLECULAR CANCER THERAPEUTICS, vol. 14, JPN6025045706, 2015, pages 1317 - 1326, ISSN: 0005728327 *

Also Published As

Publication number Publication date
US20250282894A1 (en) 2025-09-11
CN116615462A (zh) 2023-08-18
WO2022107090A1 (en) 2022-05-27
EP4247857A1 (en) 2023-09-27

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