JP2023540422A - ポリヌクレオチドを増幅するための組成物及び方法 - Google Patents

ポリヌクレオチドを増幅するための組成物及び方法 Download PDF

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JP2023540422A
JP2023540422A JP2022580750A JP2022580750A JP2023540422A JP 2023540422 A JP2023540422 A JP 2023540422A JP 2022580750 A JP2022580750 A JP 2022580750A JP 2022580750 A JP2022580750 A JP 2022580750A JP 2023540422 A JP2023540422 A JP 2023540422A
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capture primers
capture
orthogonal
primers
region
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JP2023540422A5 (enExample
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フィッシャー，ジェフリー
ベトリー，ジェイソン
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イルミナ インコーポレイテッド
イルミナ ケンブリッジ リミテッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
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  • Microbiology (AREA)
  • Biotechnology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
JP2022580750A 2020-09-14 2021-08-27 ポリヌクレオチドを増幅するための組成物及び方法 Pending JP2023540422A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14
PCT/US2021/048064 WO2022055729A1 (en) 2020-09-14 2021-08-27 Compositions and methods for amplifying polynucleotides

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JP2023540422A true JP2023540422A (ja) 2023-09-25
JP2023540422A5 JP2023540422A5 (enExample) 2024-09-03

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US (2) US11913067B2 (enExample)
EP (1) EP4211269B1 (enExample)
JP (1) JP2023540422A (enExample)
KR (1) KR20230069047A (enExample)
CN (1) CN115777024A (enExample)
AU (1) AU2021341688B2 (enExample)
BR (1) BR112022026860A2 (enExample)
CA (1) CA3182296A1 (enExample)
ES (1) ES3007341T3 (enExample)
IL (1) IL299327A (enExample)
MX (1) MX2022014724A (enExample)
TW (1) TW202225412A (enExample)
WO (1) WO2022055729A1 (enExample)
ZA (1) ZA202213367B (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022192083A1 (en) * 2021-03-09 2022-09-15 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
US20250236865A1 (en) * 2021-10-20 2025-07-24 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
EP4372101A1 (en) * 2022-11-02 2024-05-22 EsBiolab LLC Methods and devices of generating clusters of amplicons
WO2024216033A1 (en) * 2023-04-14 2024-10-17 Illumina, Inc. Capturing and amplifying polynucleotides

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011025477A1 (en) * 2009-08-25 2011-03-03 Illumina, Inc. Methods for selecting and amplifying polynucleotides
WO2012025250A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Ltd. Methods for paired - end sequencing of polynucleotides
WO2015189637A1 (en) * 2014-06-14 2015-12-17 Illumina Cambridge Limited Methods of increasing sequencing accuracy
JP2017533710A (ja) * 2014-11-11 2017-11-16 イルミナ ケンブリッジ リミテッド 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2840148B1 (en) * 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
DK3234187T3 (da) * 2014-12-15 2021-05-25 Illumina Inc Fremgangsmåde til enkeltmolekyleanbringelse på et substrat
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
AU2019368024B2 (en) * 2018-10-24 2026-02-12 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
WO2021173666A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
PL3969618T3 (pl) * 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011025477A1 (en) * 2009-08-25 2011-03-03 Illumina, Inc. Methods for selecting and amplifying polynucleotides
WO2012025250A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Ltd. Methods for paired - end sequencing of polynucleotides
WO2015189637A1 (en) * 2014-06-14 2015-12-17 Illumina Cambridge Limited Methods of increasing sequencing accuracy
JP2017533710A (ja) * 2014-11-11 2017-11-16 イルミナ ケンブリッジ リミテッド 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority

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ES3007341T3 (en) 2025-03-19
AU2021341688B2 (en) 2025-09-18
MX2022014724A (es) 2023-03-06
EP4211269A1 (en) 2023-07-19
US20240240243A1 (en) 2024-07-18
US20220090187A1 (en) 2022-03-24
IL299327A (en) 2023-02-01
TW202225412A (zh) 2022-07-01
US11913067B2 (en) 2024-02-27
EP4211269B1 (en) 2024-11-06
ZA202213367B (en) 2025-03-26
AU2021341688A1 (en) 2023-01-05
WO2022055729A1 (en) 2022-03-17
BR112022026860A2 (pt) 2023-04-11
CA3182296A1 (en) 2022-03-17
KR20230069047A (ko) 2023-05-18
CN115777024A (zh) 2023-03-10

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