CN115777024A - 用于扩增多核苷酸的组合物和方法 - Google Patents

用于扩增多核苷酸的组合物和方法 Download PDF

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Publication number
CN115777024A
CN115777024A CN202180046824.0A CN202180046824A CN115777024A CN 115777024 A CN115777024 A CN 115777024A CN 202180046824 A CN202180046824 A CN 202180046824A CN 115777024 A CN115777024 A CN 115777024A
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capture primers
orthogonal
capture
primers
region
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Chinese (zh)
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J·费希尔
J·贝特利
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Illumina Cambridge Ltd
Illumina Inc
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Illumina Cambridge Ltd
Illumina Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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CN202180046824.0A 2020-09-14 2021-08-27 用于扩增多核苷酸的组合物和方法 Pending CN115777024A (zh)

Applications Claiming Priority (3)

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US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14
PCT/US2021/048064 WO2022055729A1 (en) 2020-09-14 2021-08-27 Compositions and methods for amplifying polynucleotides

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CN115777024A true CN115777024A (zh) 2023-03-10

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US (2) US11913067B2 (enExample)
EP (1) EP4211269B1 (enExample)
JP (1) JP2023540422A (enExample)
KR (1) KR20230069047A (enExample)
CN (1) CN115777024A (enExample)
AU (1) AU2021341688B2 (enExample)
BR (1) BR112022026860A2 (enExample)
CA (1) CA3182296A1 (enExample)
ES (1) ES3007341T3 (enExample)
IL (1) IL299327A (enExample)
MX (1) MX2022014724A (enExample)
TW (1) TW202225412A (enExample)
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EP4305193B1 (en) * 2021-03-09 2025-09-17 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
WO2023069927A1 (en) * 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
US20240167088A1 (en) * 2022-11-02 2024-05-23 Esbiolab Llc Methods and devices of generating clusters of amplicons
CN117987522A (zh) * 2023-02-08 2024-05-07 深圳市真迈生物科技有限公司 测序方法
EP4695420A1 (en) * 2023-04-14 2026-02-18 Illumina, Inc. Capturing and amplifying polynucleotides

Citations (3)

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US20120053063A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Limited Methods for sequencing polynucleotides
US20150056662A1 (en) * 2013-08-23 2015-02-26 454 Life Sciences Corporation System and Method for Nucleic Acid Amplification
US20170137876A1 (en) * 2014-06-14 2017-05-18 Illumina Cambridge Limited Methods of increasing sequencing accuracy

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GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US8999642B2 (en) * 2008-03-10 2015-04-07 Illumina, Inc. Methods for selecting and amplifying polynucleotides
CN102597256B (zh) * 2009-08-25 2014-12-03 伊鲁米那股份有限公司 选择和扩增多核苷酸的方法
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
PL3218511T3 (pl) 2014-11-11 2020-10-19 Illumina Cambridge Limited Sposoby i macierze do wytwarzania i sekwencjonowania monoklonalnych klastrów kwasu nukleinowego
PT3234187T (pt) * 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CA3113808A1 (en) * 2018-10-24 2020-04-30 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
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US20220316003A1 (en) * 2020-03-09 2022-10-06 Illumina, Inc. Methods for sequencing polynucleotides

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US20120053063A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Limited Methods for sequencing polynucleotides
US20150056662A1 (en) * 2013-08-23 2015-02-26 454 Life Sciences Corporation System and Method for Nucleic Acid Amplification
US20170137876A1 (en) * 2014-06-14 2017-05-18 Illumina Cambridge Limited Methods of increasing sequencing accuracy

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US20240240243A1 (en) 2024-07-18
BR112022026860A2 (pt) 2023-04-11
EP4211269A1 (en) 2023-07-19
MX2022014724A (es) 2023-03-06
AU2021341688B2 (en) 2025-09-18
EP4211269B1 (en) 2024-11-06
AU2021341688A1 (en) 2023-01-05
ES3007341T3 (en) 2025-03-19
JP2023540422A (ja) 2023-09-25
IL299327A (en) 2023-02-01
WO2022055729A1 (en) 2022-03-17
US11913067B2 (en) 2024-02-27
ZA202213367B (en) 2025-03-26
TW202225412A (zh) 2022-07-01
CA3182296A1 (en) 2022-03-17
KR20230069047A (ko) 2023-05-18
US20220090187A1 (en) 2022-03-24

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