CN115777024A - 用于扩增多核苷酸的组合物和方法 - Google Patents

用于扩增多核苷酸的组合物和方法 Download PDF

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Publication number
CN115777024A
CN115777024A CN202180046824.0A CN202180046824A CN115777024A CN 115777024 A CN115777024 A CN 115777024A CN 202180046824 A CN202180046824 A CN 202180046824A CN 115777024 A CN115777024 A CN 115777024A
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capture primers
orthogonal
capture
primers
region
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Chinese (zh)
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J·费希尔
J·贝特利
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Illumina Cambridge Ltd
Illumina Inc
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Illumina Cambridge Ltd
Illumina Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/6869—Methods for sequencing
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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CN202180046824.0A 2020-09-14 2021-08-27 用于扩增多核苷酸的组合物和方法 Pending CN115777024A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14
PCT/US2021/048064 WO2022055729A1 (en) 2020-09-14 2021-08-27 Compositions and methods for amplifying polynucleotides

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CN115777024A true CN115777024A (zh) 2023-03-10

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EP (1) EP4211269B1 (enExample)
JP (1) JP2023540422A (enExample)
KR (1) KR20230069047A (enExample)
CN (1) CN115777024A (enExample)
AU (1) AU2021341688B2 (enExample)
BR (1) BR112022026860A2 (enExample)
CA (1) CA3182296A1 (enExample)
ES (1) ES3007341T3 (enExample)
IL (1) IL299327A (enExample)
MX (1) MX2022014724A (enExample)
TW (1) TW202225412A (enExample)
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Families Citing this family (5)

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WO2022192083A1 (en) * 2021-03-09 2022-09-15 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
US20250236865A1 (en) * 2021-10-20 2025-07-24 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
EP4372101A1 (en) * 2022-11-02 2024-05-22 EsBiolab LLC Methods and devices of generating clusters of amplicons
WO2024216033A1 (en) * 2023-04-14 2024-10-17 Illumina, Inc. Capturing and amplifying polynucleotides

Citations (3)

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US20120053063A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Limited Methods for sequencing polynucleotides
US20150056662A1 (en) * 2013-08-23 2015-02-26 454 Life Sciences Corporation System and Method for Nucleic Acid Amplification
US20170137876A1 (en) * 2014-06-14 2017-05-18 Illumina Cambridge Limited Methods of increasing sequencing accuracy

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GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
PT2669387T (pt) * 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
PT3218511T (pt) * 2014-11-11 2020-07-23 Illumina Cambridge Ltd Métodos e dispositivos para produção e sequenciamento de grupos monoclonais de ácido nucleico
DK3234187T3 (da) * 2014-12-15 2021-05-25 Illumina Inc Fremgangsmåde til enkeltmolekyleanbringelse på et substrat
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
AU2019368024B2 (en) * 2018-10-24 2026-02-12 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
DK3899037T3 (da) * 2018-12-19 2023-11-06 Illumina Inc Fremgangsmåder til forbedring af polynukleotidklyngeklonalitetsprioritet
WO2021173666A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
PL3969618T3 (pl) * 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20120053063A1 (en) * 2010-08-27 2012-03-01 Illumina Cambridge Limited Methods for sequencing polynucleotides
US20150056662A1 (en) * 2013-08-23 2015-02-26 454 Life Sciences Corporation System and Method for Nucleic Acid Amplification
US20170137876A1 (en) * 2014-06-14 2017-05-18 Illumina Cambridge Limited Methods of increasing sequencing accuracy

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AU2021341688B2 (en) 2025-09-18
MX2022014724A (es) 2023-03-06
EP4211269A1 (en) 2023-07-19
US20240240243A1 (en) 2024-07-18
US20220090187A1 (en) 2022-03-24
IL299327A (en) 2023-02-01
TW202225412A (zh) 2022-07-01
US11913067B2 (en) 2024-02-27
EP4211269B1 (en) 2024-11-06
ZA202213367B (en) 2025-03-26
AU2021341688A1 (en) 2023-01-05
WO2022055729A1 (en) 2022-03-17
BR112022026860A2 (pt) 2023-04-11
JP2023540422A (ja) 2023-09-25
CA3182296A1 (en) 2022-03-17
KR20230069047A (ko) 2023-05-18

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