TW202225412A - 用於擴增聚核苷酸之組合物及方法 - Google Patents

用於擴增聚核苷酸之組合物及方法 Download PDF

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TW202225412A
TW202225412A TW110133695A TW110133695A TW202225412A TW 202225412 A TW202225412 A TW 202225412A TW 110133695 A TW110133695 A TW 110133695A TW 110133695 A TW110133695 A TW 110133695A TW 202225412 A TW202225412 A TW 202225412A
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capture primers
orthogonal
primers
capture
region
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TW110133695A
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English (en)
Chinese (zh)
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傑森 貝特里
傑佛瑞 費雪
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美商宜曼達股份有限公司
英商伊路米納劍橋有限公司
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Publication of TW202225412A publication Critical patent/TW202225412A/zh

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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
TW110133695A 2020-09-14 2021-09-10 用於擴增聚核苷酸之組合物及方法 TW202225412A (zh)

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US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14

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US (2) US11913067B2 (enExample)
EP (1) EP4211269B1 (enExample)
JP (1) JP2023540422A (enExample)
KR (1) KR20230069047A (enExample)
CN (1) CN115777024A (enExample)
AU (1) AU2021341688B2 (enExample)
BR (1) BR112022026860A2 (enExample)
CA (1) CA3182296A1 (enExample)
ES (1) ES3007341T3 (enExample)
IL (1) IL299327A (enExample)
MX (1) MX2022014724A (enExample)
TW (1) TW202225412A (enExample)
WO (1) WO2022055729A1 (enExample)
ZA (1) ZA202213367B (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
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WO2022192083A1 (en) * 2021-03-09 2022-09-15 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
US20250236865A1 (en) * 2021-10-20 2025-07-24 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
EP4372101A1 (en) * 2022-11-02 2024-05-22 EsBiolab LLC Methods and devices of generating clusters of amplicons
WO2024216033A1 (en) * 2023-04-14 2024-10-17 Illumina, Inc. Capturing and amplifying polynucleotides

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
PT2669387T (pt) * 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2840148B1 (en) * 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
PT3218511T (pt) * 2014-11-11 2020-07-23 Illumina Cambridge Ltd Métodos e dispositivos para produção e sequenciamento de grupos monoclonais de ácido nucleico
DK3234187T3 (da) * 2014-12-15 2021-05-25 Illumina Inc Fremgangsmåde til enkeltmolekyleanbringelse på et substrat
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
AU2019368024B2 (en) * 2018-10-24 2026-02-12 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
DK3899037T3 (da) * 2018-12-19 2023-11-06 Illumina Inc Fremgangsmåder til forbedring af polynukleotidklyngeklonalitetsprioritet
WO2021173666A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
PL3969618T3 (pl) * 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów

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ES3007341T3 (en) 2025-03-19
AU2021341688B2 (en) 2025-09-18
MX2022014724A (es) 2023-03-06
EP4211269A1 (en) 2023-07-19
US20240240243A1 (en) 2024-07-18
US20220090187A1 (en) 2022-03-24
IL299327A (en) 2023-02-01
US11913067B2 (en) 2024-02-27
EP4211269B1 (en) 2024-11-06
ZA202213367B (en) 2025-03-26
AU2021341688A1 (en) 2023-01-05
WO2022055729A1 (en) 2022-03-17
BR112022026860A2 (pt) 2023-04-11
JP2023540422A (ja) 2023-09-25
CA3182296A1 (en) 2022-03-17
KR20230069047A (ko) 2023-05-18
CN115777024A (zh) 2023-03-10

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