TW202225412A - 用於擴增聚核苷酸之組合物及方法 - Google Patents

用於擴增聚核苷酸之組合物及方法 Download PDF

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TW202225412A
TW202225412A TW110133695A TW110133695A TW202225412A TW 202225412 A TW202225412 A TW 202225412A TW 110133695 A TW110133695 A TW 110133695A TW 110133695 A TW110133695 A TW 110133695A TW 202225412 A TW202225412 A TW 202225412A
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capture primers
orthogonal
primers
capture
region
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TW110133695A
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Chinese (zh)
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傑森 貝特里
傑佛瑞 費雪
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美商宜曼達股份有限公司
英商伊路米納劍橋有限公司
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Publication of TW202225412A publication Critical patent/TW202225412A/zh

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
TW110133695A 2020-09-14 2021-09-10 用於擴增聚核苷酸之組合物及方法 TW202225412A (zh)

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US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14

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US (2) US11913067B2 (enExample)
EP (1) EP4211269B1 (enExample)
JP (1) JP2023540422A (enExample)
KR (1) KR20230069047A (enExample)
CN (1) CN115777024A (enExample)
AU (1) AU2021341688B2 (enExample)
BR (1) BR112022026860A2 (enExample)
CA (1) CA3182296A1 (enExample)
ES (1) ES3007341T3 (enExample)
IL (1) IL299327A (enExample)
MX (1) MX2022014724A (enExample)
TW (1) TW202225412A (enExample)
WO (1) WO2022055729A1 (enExample)
ZA (1) ZA202213367B (enExample)

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EP4305193B1 (en) * 2021-03-09 2025-09-17 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
WO2023069927A1 (en) * 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
US20240167088A1 (en) * 2022-11-02 2024-05-23 Esbiolab Llc Methods and devices of generating clusters of amplicons
CN117987522A (zh) * 2023-02-08 2024-05-07 深圳市真迈生物科技有限公司 测序方法
EP4695420A1 (en) * 2023-04-14 2026-02-18 Illumina, Inc. Capturing and amplifying polynucleotides

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GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US8999642B2 (en) * 2008-03-10 2015-04-07 Illumina, Inc. Methods for selecting and amplifying polynucleotides
CN102597256B (zh) * 2009-08-25 2014-12-03 伊鲁米那股份有限公司 选择和扩增多核苷酸的方法
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2840148B1 (en) * 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
PL3218511T3 (pl) 2014-11-11 2020-10-19 Illumina Cambridge Limited Sposoby i macierze do wytwarzania i sekwencjonowania monoklonalnych klastrów kwasu nukleinowego
PT3234187T (pt) * 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CA3113808A1 (en) * 2018-10-24 2020-04-30 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
DK3969613T3 (da) * 2020-02-26 2024-01-08 Illumina Inc Kits til genotypebestemmelse
US20220316003A1 (en) * 2020-03-09 2022-10-06 Illumina, Inc. Methods for sequencing polynucleotides

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US20240240243A1 (en) 2024-07-18
BR112022026860A2 (pt) 2023-04-11
EP4211269A1 (en) 2023-07-19
CN115777024A (zh) 2023-03-10
MX2022014724A (es) 2023-03-06
AU2021341688B2 (en) 2025-09-18
EP4211269B1 (en) 2024-11-06
AU2021341688A1 (en) 2023-01-05
ES3007341T3 (en) 2025-03-19
JP2023540422A (ja) 2023-09-25
IL299327A (en) 2023-02-01
WO2022055729A1 (en) 2022-03-17
US11913067B2 (en) 2024-02-27
ZA202213367B (en) 2025-03-26
CA3182296A1 (en) 2022-03-17
KR20230069047A (ko) 2023-05-18
US20220090187A1 (en) 2022-03-24

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