CA3182296A1 - Compositions and methods for amplifying polynucleotides - Google Patents
Compositions and methods for amplifying polynucleotides Download PDFInfo
- Publication number
- CA3182296A1 CA3182296A1 CA3182296A CA3182296A CA3182296A1 CA 3182296 A1 CA3182296 A1 CA 3182296A1 CA 3182296 A CA3182296 A CA 3182296A CA 3182296 A CA3182296 A CA 3182296A CA 3182296 A1 CA3182296 A1 CA 3182296A1
- Authority
- CA
- Canada
- Prior art keywords
- capture primers
- orthogonal
- capture
- primers
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063077857P | 2020-09-14 | 2020-09-14 | |
| US63/077,857 | 2020-09-14 | ||
| PCT/US2021/048064 WO2022055729A1 (en) | 2020-09-14 | 2021-08-27 | Compositions and methods for amplifying polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3182296A1 true CA3182296A1 (en) | 2022-03-17 |
Family
ID=78087470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3182296A Pending CA3182296A1 (en) | 2020-09-14 | 2021-08-27 | Compositions and methods for amplifying polynucleotides |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US11913067B2 (enExample) |
| EP (1) | EP4211269B1 (enExample) |
| JP (1) | JP2023540422A (enExample) |
| KR (1) | KR20230069047A (enExample) |
| CN (1) | CN115777024A (enExample) |
| AU (1) | AU2021341688B2 (enExample) |
| BR (1) | BR112022026860A2 (enExample) |
| CA (1) | CA3182296A1 (enExample) |
| ES (1) | ES3007341T3 (enExample) |
| IL (1) | IL299327A (enExample) |
| MX (1) | MX2022014724A (enExample) |
| TW (1) | TW202225412A (enExample) |
| WO (1) | WO2022055729A1 (enExample) |
| ZA (1) | ZA202213367B (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4305193B1 (en) * | 2021-03-09 | 2025-09-17 | Illumina, Inc. | Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers |
| WO2023069927A1 (en) * | 2021-10-20 | 2023-04-27 | Illumina, Inc. | Methods for capturing library dna for sequencing |
| US20240052406A1 (en) * | 2022-08-04 | 2024-02-15 | Singular Genomics Systems, Inc. | Competitive methods and compositions for amplifying polynucleotides |
| US20240167088A1 (en) * | 2022-11-02 | 2024-05-23 | Esbiolab Llc | Methods and devices of generating clusters of amplicons |
| CN117987522A (zh) * | 2023-02-08 | 2024-05-07 | 深圳市真迈生物科技有限公司 | 测序方法 |
| EP4695420A1 (en) * | 2023-04-14 | 2026-02-18 | Illumina, Inc. | Capturing and amplifying polynucleotides |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0514909D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Methods of nucleic acid amplification and sequencing |
| US8999642B2 (en) * | 2008-03-10 | 2015-04-07 | Illumina, Inc. | Methods for selecting and amplifying polynucleotides |
| CN102597256B (zh) * | 2009-08-25 | 2014-12-03 | 伊鲁米那股份有限公司 | 选择和扩增多核苷酸的方法 |
| US9029103B2 (en) * | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| EP2840148B1 (en) * | 2013-08-23 | 2019-04-03 | F. Hoffmann-La Roche AG | Methods for nucleic acid amplification |
| GB201410646D0 (en) | 2014-06-14 | 2014-07-30 | Illumina Cambridge Ltd | Methods of increasing sequencing accuracy |
| PL3218511T3 (pl) | 2014-11-11 | 2020-10-19 | Illumina Cambridge Limited | Sposoby i macierze do wytwarzania i sekwencjonowania monoklonalnych klastrów kwasu nukleinowego |
| PT3234187T (pt) * | 2014-12-15 | 2021-05-26 | Illumina Inc | Método para colocação de moleculas de alvo unico num substrato |
| ITUA20162640A1 (it) * | 2016-04-15 | 2017-10-15 | Menarini Silicon Biosystems Spa | Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo |
| WO2018071522A1 (en) * | 2016-10-11 | 2018-04-19 | Life Technologies Corporation | Rapid amplification of nucleic acids |
| GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
| CA3113808A1 (en) * | 2018-10-24 | 2020-04-30 | University Of Washington | Methods and kits for depletion and enrichment of nucleic acid sequences |
| BR112020026667A2 (pt) * | 2018-12-19 | 2021-07-27 | Illumina, Inc. | métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo |
| DK3969613T3 (da) * | 2020-02-26 | 2024-01-08 | Illumina Inc | Kits til genotypebestemmelse |
| US20220316003A1 (en) * | 2020-03-09 | 2022-10-06 | Illumina, Inc. | Methods for sequencing polynucleotides |
-
2021
- 2021-08-27 MX MX2022014724A patent/MX2022014724A/es unknown
- 2021-08-27 ES ES21790611T patent/ES3007341T3/es active Active
- 2021-08-27 AU AU2021341688A patent/AU2021341688B2/en active Active
- 2021-08-27 WO PCT/US2021/048064 patent/WO2022055729A1/en not_active Ceased
- 2021-08-27 BR BR112022026860A patent/BR112022026860A2/pt not_active Application Discontinuation
- 2021-08-27 JP JP2022580750A patent/JP2023540422A/ja active Pending
- 2021-08-27 IL IL299327A patent/IL299327A/en unknown
- 2021-08-27 KR KR1020227045843A patent/KR20230069047A/ko active Pending
- 2021-08-27 US US17/459,966 patent/US11913067B2/en active Active
- 2021-08-27 EP EP21790611.4A patent/EP4211269B1/en active Active
- 2021-08-27 CA CA3182296A patent/CA3182296A1/en active Pending
- 2021-08-27 CN CN202180046824.0A patent/CN115777024A/zh active Pending
- 2021-09-10 TW TW110133695A patent/TW202225412A/zh unknown
-
2022
- 2022-12-09 ZA ZA2022/13367A patent/ZA202213367B/en unknown
-
2024
- 2024-01-22 US US18/419,082 patent/US20240240243A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20240240243A1 (en) | 2024-07-18 |
| BR112022026860A2 (pt) | 2023-04-11 |
| EP4211269A1 (en) | 2023-07-19 |
| CN115777024A (zh) | 2023-03-10 |
| MX2022014724A (es) | 2023-03-06 |
| AU2021341688B2 (en) | 2025-09-18 |
| EP4211269B1 (en) | 2024-11-06 |
| AU2021341688A1 (en) | 2023-01-05 |
| ES3007341T3 (en) | 2025-03-19 |
| JP2023540422A (ja) | 2023-09-25 |
| IL299327A (en) | 2023-02-01 |
| WO2022055729A1 (en) | 2022-03-17 |
| US11913067B2 (en) | 2024-02-27 |
| ZA202213367B (en) | 2025-03-26 |
| TW202225412A (zh) | 2022-07-01 |
| KR20230069047A (ko) | 2023-05-18 |
| US20220090187A1 (en) | 2022-03-24 |
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