JP2023522234A - 末端デオキシヌクレオチジルトランスフェラーゼ変異体およびその使用 - Google Patents
末端デオキシヌクレオチジルトランスフェラーゼ変異体およびその使用 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
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- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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| EP20170405 | 2020-04-20 | ||
| EP20170405.3 | 2020-04-20 | ||
| PCT/EP2021/059861 WO2021213903A1 (en) | 2020-04-20 | 2021-04-16 | Terminal deoxynucleotidyl transferase variants and uses thereof |
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| WO2021058438A1 (en) | 2019-09-23 | 2021-04-01 | Dna Script | Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides |
| CA3234383A1 (en) | 2021-10-15 | 2023-04-27 | Codexis, Inc. | Engineered terminal deoxynucleotidyl transferase variants |
| WO2023187214A1 (en) | 2022-03-31 | 2023-10-05 | Ribbon Biolabs Gmbh | Biopolymer synthesis |
| EP4735621A1 (en) | 2023-06-30 | 2026-05-06 | DNA Script | Nucleic acid synthesis on reusable support |
| GB2635104A (en) * | 2023-09-08 | 2025-05-07 | Evonetix Ltd | Enzymes and methods |
| WO2025088024A1 (en) * | 2023-10-24 | 2025-05-01 | Dna Script | Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof |
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| WO2026008717A2 (en) | 2024-07-02 | 2026-01-08 | Dna Script | Method for enzymatic synthesis of polynucleotides |
| WO2026017834A1 (en) | 2024-07-19 | 2026-01-22 | Dna Script | Inkjet-assisted enzymatic nucleic acid synthesis and cleavage |
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| US5367066A (en) | 1984-10-16 | 1994-11-22 | Chiron Corporation | Oligonucleotides with selectably cleavable and/or abasic sites |
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5436143A (en) | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
| AU1436995A (en) | 1993-12-30 | 1995-07-17 | Chemgenes Corporation | Synthesis of propargyl modified nucleosides and phosphoramidites and their incorporation into defined sequence oligonucleotides |
| EP0776330B1 (en) | 1994-06-23 | 2003-08-20 | Affymax Technologies N.V. | Photolabile compounds and methods for their use |
| US5808045A (en) | 1994-09-02 | 1998-09-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US5763594A (en) | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
| US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| EP0967217B1 (en) | 1998-06-22 | 2005-12-21 | Affymetrix, Inc. (a California Corporation) | Reagents and methods for solid phase synthesis and display |
| ATE296310T1 (de) | 1999-03-08 | 2005-06-15 | Metrigen Inc | Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür |
| US6664097B2 (en) | 2000-05-23 | 2003-12-16 | North Carolina State University | Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US20060086538A1 (en) | 2002-07-08 | 2006-04-27 | Shell Oil Company | Choke for controlling the flow of drilling mud |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US8212020B2 (en) | 2005-03-11 | 2012-07-03 | Steven Albert Benner | Reagents for reversibly terminating primer extension |
| US7544794B1 (en) | 2005-03-11 | 2009-06-09 | Steven Albert Benner | Method for sequencing DNA and RNA by synthesis |
| WO2008042067A2 (en) | 2006-09-28 | 2008-04-10 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| US8034923B1 (en) | 2009-03-27 | 2011-10-11 | Steven Albert Benner | Reagents for reversibly terminating primer extension |
| EP2876166B1 (en) | 2013-11-20 | 2016-12-14 | Roche Diagnostics GmbH | New compound for sequencing by synthesis |
| FR3020071B1 (fr) * | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| GB201502152D0 (en) * | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| FR3052462A1 (fr) | 2016-06-14 | 2017-12-15 | Dna Script | Variants d'une adn polymerase de la famille polx |
| US10472383B2 (en) | 2017-03-16 | 2019-11-12 | Steven A Benner | Nucleoside triphosphates with stable aminoxy groups |
| AU2018272302A1 (en) * | 2017-05-26 | 2019-12-19 | Nuclera Nucleics Ltd | Use of terminal transferase enzyme in nucleic acid synthesis |
| US20190078065A1 (en) | 2017-09-08 | 2019-03-14 | Sigma-Aldrich Co. Llc | Modified dna polymerases |
| US10435676B2 (en) * | 2018-01-08 | 2019-10-08 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) * | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| CA3119845A1 (en) * | 2018-11-14 | 2020-05-22 | Dna Script | Terminal deoxynucleotidyl transferase variants and uses thereof |
| SG11202105441WA (en) * | 2018-12-13 | 2021-06-29 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| WO2020165137A1 (en) * | 2019-02-12 | 2020-08-20 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
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| CN115427559A (zh) | 2022-12-02 |
| AU2021260014A1 (en) | 2022-11-17 |
| CA3175780A1 (en) | 2021-10-28 |
| IL297425A (en) | 2022-12-01 |
| KR20230002825A (ko) | 2023-01-05 |
| WO2021213903A1 (en) | 2021-10-28 |
| US20230159903A1 (en) | 2023-05-25 |
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