CN115427559A - 末端脱氧核苷酸转移酶变体及其用途 - Google Patents

末端脱氧核苷酸转移酶变体及其用途 Download PDF

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Publication number
CN115427559A
CN115427559A CN202180029834.3A CN202180029834A CN115427559A CN 115427559 A CN115427559 A CN 115427559A CN 202180029834 A CN202180029834 A CN 202180029834A CN 115427559 A CN115427559 A CN 115427559A
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glu
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ala
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Chinese (zh)
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米哈伊尔·索斯金
伊莉斯·钱皮恩
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DNA Script SAS
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DNA Script SAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180029834.3A 2020-04-20 2021-04-16 末端脱氧核苷酸转移酶变体及其用途 Pending CN115427559A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170405 2020-04-20
EP20170405.3 2020-04-20
PCT/EP2021/059861 WO2021213903A1 (en) 2020-04-20 2021-04-16 Terminal deoxynucleotidyl transferase variants and uses thereof

Publications (1)

Publication Number Publication Date
CN115427559A true CN115427559A (zh) 2022-12-02

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Family Applications (1)

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CN202180029834.3A Pending CN115427559A (zh) 2020-04-20 2021-04-16 末端脱氧核苷酸转移酶变体及其用途

Country Status (9)

Country Link
US (1) US20230159903A1 (https=)
EP (1) EP4139446A1 (https=)
JP (1) JP2023522234A (https=)
KR (1) KR20230002825A (https=)
CN (1) CN115427559A (https=)
AU (1) AU2021260014A1 (https=)
CA (1) CA3175780A1 (https=)
IL (1) IL297425A (https=)
WO (1) WO2021213903A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021058438A1 (en) 2019-09-23 2021-04-01 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023187214A1 (en) 2022-03-31 2023-10-05 Ribbon Biolabs Gmbh Biopolymer synthesis
EP4735621A1 (en) 2023-06-30 2026-05-06 DNA Script Nucleic acid synthesis on reusable support
GB2635104A (en) * 2023-09-08 2025-05-07 Evonetix Ltd Enzymes and methods
WO2025088024A1 (en) * 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
US20250313876A1 (en) 2024-04-06 2025-10-09 Ribbon Bio GmbH Isolation of dna fragments
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

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CN107567501A (zh) * 2015-02-10 2018-01-09 核酸有限公司 新用途
US20190211315A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of Terminal Deoxynucleotidyl Transferase and Uses Thereof
US20190264248A1 (en) * 2014-04-17 2019-08-29 Dna Script Method for Synthesizing Nucleic Acids, in particular Long Nucleic Acids, Use of Said Method and Kit for Implementing Said Method
CN110997907A (zh) * 2017-05-26 2020-04-10 核酸有限公司 末端转移酶在核酸合成中的用途
WO2020120442A2 (en) * 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules

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AU1436995A (en) 1993-12-30 1995-07-17 Chemgenes Corporation Synthesis of propargyl modified nucleosides and phosphoramidites and their incorporation into defined sequence oligonucleotides
EP0776330B1 (en) 1994-06-23 2003-08-20 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
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CN107567501A (zh) * 2015-02-10 2018-01-09 核酸有限公司 新用途
CN110997907A (zh) * 2017-05-26 2020-04-10 核酸有限公司 末端转移酶在核酸合成中的用途
US20190211315A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of Terminal Deoxynucleotidyl Transferase and Uses Thereof
WO2020120442A2 (en) * 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules

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Title
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EP4139446A1 (en) 2023-03-01
JP2023522234A (ja) 2023-05-29
AU2021260014A1 (en) 2022-11-17
CA3175780A1 (en) 2021-10-28
IL297425A (en) 2022-12-01
KR20230002825A (ko) 2023-01-05
WO2021213903A1 (en) 2021-10-28
US20230159903A1 (en) 2023-05-25

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Application publication date: 20221202