KR20230002825A - 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도 - Google Patents

말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도 Download PDF

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KR20230002825A
KR20230002825A KR1020227040201A KR20227040201A KR20230002825A KR 20230002825 A KR20230002825 A KR 20230002825A KR 1020227040201 A KR1020227040201 A KR 1020227040201A KR 20227040201 A KR20227040201 A KR 20227040201A KR 20230002825 A KR20230002825 A KR 20230002825A
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Korean (ko)
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미카엘 소스킨
엘리스 챰피온
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디엔에이 스크립트
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1264DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07031DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/32Chemical structure of the sugar
    • C12N2310/3212'-O-R Modification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020227040201A 2020-04-20 2021-04-16 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도 Withdrawn KR20230002825A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170405 2020-04-20
EP20170405.3 2020-04-20
PCT/EP2021/059861 WO2021213903A1 (en) 2020-04-20 2021-04-16 Terminal deoxynucleotidyl transferase variants and uses thereof

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KR20230002825A true KR20230002825A (ko) 2023-01-05

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KR1020227040201A Withdrawn KR20230002825A (ko) 2020-04-20 2021-04-16 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도

Country Status (9)

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US (1) US20230159903A1 (https=)
EP (1) EP4139446A1 (https=)
JP (1) JP2023522234A (https=)
KR (1) KR20230002825A (https=)
CN (1) CN115427559A (https=)
AU (1) AU2021260014A1 (https=)
CA (1) CA3175780A1 (https=)
IL (1) IL297425A (https=)
WO (1) WO2021213903A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021058438A1 (en) 2019-09-23 2021-04-01 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023187214A1 (en) 2022-03-31 2023-10-05 Ribbon Biolabs Gmbh Biopolymer synthesis
EP4735621A1 (en) 2023-06-30 2026-05-06 DNA Script Nucleic acid synthesis on reusable support
GB2635104A (en) * 2023-09-08 2025-05-07 Evonetix Ltd Enzymes and methods
WO2025088024A1 (en) * 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
US20250313876A1 (en) 2024-04-06 2025-10-09 Ribbon Bio GmbH Isolation of dna fragments
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

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US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
AU1436995A (en) 1993-12-30 1995-07-17 Chemgenes Corporation Synthesis of propargyl modified nucleosides and phosphoramidites and their incorporation into defined sequence oligonucleotides
EP0776330B1 (en) 1994-06-23 2003-08-20 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
EP0967217B1 (en) 1998-06-22 2005-12-21 Affymetrix, Inc. (a California Corporation) Reagents and methods for solid phase synthesis and display
ATE296310T1 (de) 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
US6664097B2 (en) 2000-05-23 2003-12-16 North Carolina State University Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20060086538A1 (en) 2002-07-08 2006-04-27 Shell Oil Company Choke for controlling the flow of drilling mud
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
US7544794B1 (en) 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8034923B1 (en) 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
EP2876166B1 (en) 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
FR3020071B1 (fr) * 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
GB201502152D0 (en) * 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
FR3052462A1 (fr) 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
US10472383B2 (en) 2017-03-16 2019-11-12 Steven A Benner Nucleoside triphosphates with stable aminoxy groups
AU2018272302A1 (en) * 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
US20190078065A1 (en) 2017-09-08 2019-03-14 Sigma-Aldrich Co. Llc Modified dna polymerases
US10435676B2 (en) * 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) * 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
CA3119845A1 (en) * 2018-11-14 2020-05-22 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
SG11202105441WA (en) * 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
WO2020165137A1 (en) * 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Also Published As

Publication number Publication date
EP4139446A1 (en) 2023-03-01
JP2023522234A (ja) 2023-05-29
CN115427559A (zh) 2022-12-02
AU2021260014A1 (en) 2022-11-17
CA3175780A1 (en) 2021-10-28
IL297425A (en) 2022-12-01
WO2021213903A1 (en) 2021-10-28
US20230159903A1 (en) 2023-05-25

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Patent event date: 20221117

Patent event code: PA01051R01D

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Patent event code: PC12021R01D

Patent event date: 20241126

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